International Journal of Biochemistry最新文献_第10页

The relationship between the trimethylation of lysine 77 and cytochrome c metabolism in Saccharomyces cerevisiae 酿酒酵母赖氨酸77三甲基化与细胞色素c代谢的关系

International Journal of Biochemistry Pub Date : 1994-05-01 DOI: 10.1016/0020-711X(94)90172-4

Karamba J. Ceesay, Lynn R. Rider, Lawrence W. Bergman , Martin T. Tuck

{"title":"The relationship between the trimethylation of lysine 77 and cytochrome c metabolism in Saccharomyces cerevisiae","authors":"Karamba J. Ceesay, Lynn R. Rider, Lawrence W. Bergman , Martin T. Tuck","doi":"10.1016/0020-711X(94)90172-4","DOIUrl":"10.1016/0020-711X(94)90172-4","url":null,"abstract":"<div><ul><li>1.1. Site directed mutations were constructed in the yeast iso-1-cytochrome c gene adjacent to the lysine 77 (methylation site) codon.</li><li>2.2. These mutant genes were then cloned and transformed into the S. cerevisiae strain B-6642 which contains a deficiency in the iso-1-cytochrome c gene.</li><li>3.3. The resulting transformants were screened for cytochrome c production using gel electrophoresis.</li><li>4.4. Amino acid analysis of the mutated cytochromes c demonstrated varying levels of trimethyllysine formation, depending on the nature of the site directed mutation.</li><li>5.5. The resulting transformants were then used as tools in order to investigate the relationship between trimethyllysine formation and various aspects of cytochrome c metabolism including protein stability and heme conjugation.</li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 5","pages":"Pages 721-734"},"PeriodicalIF":0.0,"publicationDate":"1994-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90172-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19004218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Ependymins and their potential role in neuroplasticity and regeneration: Calcium-binding meningeal glycoproteins of the cerebrospinal fluid and extracellular matrix 室管素及其在神经可塑性和再生中的潜在作用:脑脊液和细胞外基质中钙结合的脑膜糖蛋白

International Journal of Biochemistry Pub Date : 1994-05-01 DOI: 10.1016/0020-711X(94)90160-0

Werner Hoffmann

引用次数: 14

Kinetics of manganese reconstitution and thiol group exposition in dialyzed rat mammary gland arginase 大鼠乳腺精氨酸酶透析中锰重构和巯基暴露动力学

International Journal of Biochemistry Pub Date : 1994-05-01 DOI: 10.1016/0020-711X(94)90165-1

J.M. Fuentes, M.L. Campo, G. Soler

引用次数: 19

Binding of meso-tetra(4-sulfonatophenyl)porphine to haemopexin and albumin studied by spectroscopy methods 用光谱学方法研究了中四(4-磺酰基)卟啉与血红素和白蛋白的结合

International Journal of Biochemistry Pub Date : 1994-05-01 DOI: 10.1016/0020-711X(94)90162-7

J. Bartošová, I. Kalousek, Z. Hrkal

{"title":"Binding of meso-tetra(4-sulfonatophenyl)porphine to haemopexin and albumin studied by spectroscopy methods","authors":"J. Bartošová, I. Kalousek, Z. Hrkal","doi":"10.1016/0020-711X(94)90162-7","DOIUrl":"10.1016/0020-711X(94)90162-7","url":null,"abstract":"<div><ul><li>1.1. The interaction of haemopexin and albumin with TPPS4 was studied by measuring the absorption and fluorescence spectra. Haemopexin was found to have one strong TPPS4 binding center (Ka = 3 × 107M−1).</li><li>2.2. Haem-haemopexin complex appears to have no specific binding site for TPPS4. Occupation of the specific binding center of haemopexin molecule by a haem abolishes TPPS4 binding.</li><li>3.3. Albumin was found to possess one strong TPPS4 binding center (Ka = 3 × 106M−1) besides two or three weak binding sites (Ka = 2 × 105M−1).</li><li>4.4. Haern-albumin complex possesses only one weak TPPS4 binding site (Ka = 7 × lO5M−1). These observations suggest identity of primary binding sites of TPPS4 and haem on albumin molecule.</li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 5","pages":"Pages 631-637"},"PeriodicalIF":0.0,"publicationDate":"1994-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90162-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18998523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Evidence that the 5' end of rabbit globin mRNA is hybridized with its 3' end 证明兔珠蛋白mRNA的5'端与其3'端杂交

International Journal of Biochemistry Pub Date : 1994-05-01 DOI: 10.1016/0020-711X(94)90170-8

Harvey N. Rubin, Randy S. Stefanko , Mostafa N. Halim

引用次数: 0

Carnitine and derivatives in the central nervous system of chick embryo 鸡胚中枢神经系统中的肉毒碱及其衍生物

International Journal of Biochemistry Pub Date : 1994-05-01 DOI: 10.1016/0020-711X(94)90171-6

P. Chiodi , B. Clani , S. Kentroti , F. Maccari , A. Vernadakis , L. Angelucci , M.T. Ramacci

引用次数: 20

Prostaglandin E2/parathyroid hormone-induced suppression of alkaline phosphatase activity is mediated by protein kinase C 前列腺素E2/甲状旁腺激素诱导的碱性磷酸酶活性抑制是由蛋白激酶C介导的

International Journal of Biochemistry Pub Date : 1994-05-01 DOI: 10.1016/0020-711X(94)90163-5

Tsuneo Miya , Masatoshi Tagawa , Noriko Kato , Kikuo Takahashi , Kenichi Sato , Shinji Fujimura

引用次数: 0

Thrombin induces S-d-lactoylglutathione accumulation by enhancing platelet glycolytic pathway 凝血酶通过增强血小板糖酵解途径诱导s -d-乳酸谷胱甘肽积累

International Journal of Biochemistry Pub Date : 1994-05-01 DOI: 10.1016/0020-711X(94)90166-X

Giuliana Leoncini, Enrica Buzzi

{"title":"Thrombin induces S-d-lactoylglutathione accumulation by enhancing platelet glycolytic pathway","authors":"Giuliana Leoncini, Enrica Buzzi","doi":"10.1016/0020-711X(94)90166-X","DOIUrl":"10.1016/0020-711X(94)90166-X","url":null,"abstract":"<div><ul><li>1.1. Thrombin addition to human platelets stimulates l(+)lactate formation and S-D-lactoylglutathione (SDL) accumulation.</li><li>2.2. Monoiodoacetamide decreases lactate formation and potentiates SDL accumulation through a significant increase of dihydroxyacetone phosphate and fructose 1,6bisphosphate intracellular levels both in resting and in activated platelets.</li><li>3.3. A similar effect is produced by exogenous methylglyoxal on L(+)lactate formation and SDL accumulation.</li><li>4.4. Resting platelets completely transform (1 hr at 37°C) the ketoaldehyde into d(-)lactate:</li><li>5.5. When platelets are incubated in the presence of thrombin only 60% of the ketoaldehyde is found as d(-)lactate and the accumulated S-d-lactoylglutathione represents about the 0.7% of the initial substrate.</li><li>6.6. During platelet stimulation with thrombin the hemithioacetal adduct, formed as a by-product of glycolytic pathway, can be rapidly removed for important steps of cellular activation.</li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 5","pages":"Pages 661-665"},"PeriodicalIF":0.0,"publicationDate":"1994-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90166-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19004212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Generation of C3- and C2-deuterated l-lactic acid by human erythrocytes exposed to d-[1-13C]glucose, d-[2-13c]glucose and d-[6-13C]glucose in the presence of D2O 暴露于d-[1-13C]葡萄糖、d-[2-13c]葡萄糖和d-[6-13C]葡萄糖的人红细胞在D2O存在下产生C3-和c2 -氘化l-乳酸

International Journal of Biochemistry Pub Date : 1994-05-01 DOI: 10.1016/0020-711X(94)90169-4

Willy J. Malaisse , Monique Blesemans , Rudolph Willem

{"title":"Generation of C3- and C2-deuterated l-lactic acid by human erythrocytes exposed to d-[1-13C]glucose, d-[2-13c]glucose and d-[6-13C]glucose in the presence of D2O","authors":"Willy J. Malaisse , Monique Blesemans , Rudolph Willem","doi":"10.1016/0020-711X(94)90169-4","DOIUrl":"10.1016/0020-711X(94)90169-4","url":null,"abstract":"<div><ul><li>1.1. The generation of C2- and C3-deuterated l-lactate was monitored by 13C NMR in human erythrocytes exposed to d-[1-13glucose, d-[2-13C]glucose or d-te-13C]glucose and incubated in a medium prepared in D2O.</li><li>2.2. The results suggested that the deuteration of the C1 of d-fructose 6-phosphate in the phosphoglucoisomerase reaction, the deuteration of the C1 of d-glyceraldehyde-3-phosphate in the sequence of reactions catalyzed by triose phosphate isomerase and aldolase and the deuteration of the C3 of pyruvate in the reaction catalyzed by pyruvate kinase were all lower than expected from equilibration with D2O.</li><li>3.3. Moreover, about 40% of the molecules of pyruvate generated by glycolysis apparently underwent deuteration on their C3 during interconversion of the 2-keto acid and l-alanine in the reaction catalyzed by glutamate-pyruvate transaminase.</li><li>4.4. The occurrence of the latter process was also documented in cells exposed to exogenous [3-13C]pyruvate.</li><li>5.5. This methodological approach is proposed to provide a new tool to assess in intact cells the extent of back-and-forth interconversion of selected metabolic intermediates.</li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 5","pages":"Pages 697-702"},"PeriodicalIF":0.0,"publicationDate":"1994-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90169-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19004215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Multiplicity of dog kidney high-Km aldose reductase and conversion mechanism into aldose reductase 狗肾高km醛糖还原酶的多样性及其转化为醛糖还原酶的机制

International Journal of Biochemistry Pub Date : 1994-04-01 DOI: 10.1016/0020-711X(94)90015-9

Miyako Ohta, Tsuyoshi Tanimoto, Takao Hayakawa

{"title":"Multiplicity of dog kidney high-Km aldose reductase and conversion mechanism into aldose reductase","authors":"Miyako Ohta, Tsuyoshi Tanimoto, Takao Hayakawa","doi":"10.1016/0020-711X(94)90015-9","DOIUrl":"10.1016/0020-711X(94)90015-9","url":null,"abstract":"<div><ul><li>1.1. High-Km, aldose reductase purified from dog kidney inner medulla was easily converted into aldose reductase by incubation in the neutral buffer solution.</li><li>2.2. High-Km, aldose reductase was found to be in multiple forms, and was separated into three kinds of species designated as a-, b- and c-forms by HPLC.</li><li>3.3. The a-form observed as a single peak by HPLC was assumed to be present in three forms (al-, a2- and a3-forms), one was aldose reductase (a 1-form) and the others were the precursors of aldose reductase (a2- and a3-form).</li><li>4.4. The b-form was rapidly converted into the a3-form, followed slowly by the a2-form and finally into the a 1-form.</li><li>5.5. The c-form was either directly converted into the al-form, or indirectly into the a2-form followed by the al-form.</li><li>6.6. Four kinds of species (a2-, a3-, b- and c-forms) of high-Ap, aldose reductase were finally converted into aldose reductase (al-form).</li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 4","pages":"Pages 565-573"},"PeriodicalIF":0.0,"publicationDate":"1994-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90015-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19011692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2