The relationship between the trimethylation of lysine 77 and cytochrome c metabolism in Saccharomyces cerevisiae

International Journal of Biochemistry Pub Date : 1994-05-01 Epub Date: 2003-02-05 DOI:10.1016/0020-711X(94)90172-4

Karamba J. Ceesay, Lynn R. Rider, Lawrence W. Bergman , Martin T. Tuck

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引用次数: 4

Abstract

1.
1. Site directed mutations were constructed in the yeast iso-1-cytochrome c gene adjacent to the lysine 77 (methylation site) codon.
2.
2. These mutant genes were then cloned and transformed into the S. cerevisiae strain B-6642 which contains a deficiency in the iso-1-cytochrome c gene.
3.
3. The resulting transformants were screened for cytochrome c production using gel electrophoresis.
4.
4. Amino acid analysis of the mutated cytochromes c demonstrated varying levels of trimethyllysine formation, depending on the nature of the site directed mutation.
5.
5. The resulting transformants were then used as tools in order to investigate the relationship between trimethyllysine formation and various aspects of cytochrome c metabolism including protein stability and heme conjugation.

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酿酒酵母赖氨酸77三甲基化与细胞色素c代谢的关系

1.1. 在赖氨酸77(甲基化位点)密码子附近的酵母iso-1细胞色素c基因中构建了位点定向突变。这些突变基因随后被克隆并转化到含有iso1 -细胞色素c基因缺失的酿酒葡萄球菌菌株B-6642中。利用凝胶电泳技术对转化子进行筛选，以产生细胞色素c。突变细胞色素c的氨基酸分析表明，根据位点定向突变的性质，三甲基赖氨酸的形成水平不同。然后将所得转化子用作工具，以研究三甲基赖氨酸形成与细胞色素c代谢的各个方面(包括蛋白质稳定性和血红素偶联)之间的关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International Journal of Biochemistry

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