Human sciatic nerve phospholipid profiles from non-diabetes mellitus, non-insulin-dependent diabetes mellitus and insulin-dependent diabetes mellitus individuals. A 31P NMR spectroscopy study

Abstract

• 1.

  1. Human sciatic nerve phospholipids obtained from non-diabetes mellitus (NDM), non-insulin-dependent diabetes mellitus (NIDDM), and insulin-dependent diabetes mellitus (IDDM) patients, after lower extremity amputation, were studied by ³¹P NMR spectrometry.

• 2.

  2. Nine phospholipids resonances in NDM and NIDDM groups were identified as followed: Ethanolamine plasmalogen (Epias, Chemical shift = 0.07δ); phosphatidylethanolamine (PE, 0.03δ); phosphatidylserine (PS, −0.05δ); sphingomyelin (SM, −0.09δ); lysophosphatidylcholine (LPC, −0.28δ); phosphatidylinositol (PI, −0.30δ); alkylacylphosphorylcholine (A1.PC, -0.78δ); phosphatidylcholine (PC −0.84δ), and an unknown resonance (U, 0.13δ).

• 3.

  3. In the IDDM group a resonance of lysophosphatidylinositol (LPI, 0.01δ) was detected in addition to the nine phospholipids listed above.

• 4.

  4. IDDM showed that PI and Al.PC were elevated and U was lower when compared with NDM; also, Eplas was lower when compared with NIDDM. PC was elevated and PS was lower when compared with both NDM and NIDDM.

• 5.

  5. Indices calculated from this data, showed that the choline ratio and choline/ ethanolamine ratio were elevated; while ethanolamine ratio, and myelin ratio were lower in IDDM group, when compared with both NDM and NIDDM groups.

• 6.

  6. Inactivation of the cholineacethyltransferase enzyme (ChAT) and enhancement of the phospholipidmethyltransferase enzyme (PLMT), secondary to an insulin deficiency, are proposed as an interpretation of these findings.