International Journal of Biochemistry最新文献_第5页

Prostaglandin E1 and analogs of prostacyclin influencing superoxide production by the human neutrophil nadph oxidase system 前列腺素E1和前列腺素类似物影响人中性粒细胞nadph氧化酶系统产生超氧化物

International Journal of Biochemistry Pub Date : 1994-08-01 DOI: 10.1016/0020-711X(94)90071-X

Shigenobu Umeki

{"title":"Prostaglandin E1 and analogs of prostacyclin influencing superoxide production by the human neutrophil nadph oxidase system","authors":"Shigenobu Umeki","doi":"10.1016/0020-711X(94)90071-X","DOIUrl":"https://doi.org/10.1016/0020-711X(94)90071-X","url":null,"abstract":"<div><ul><li>1.1. The effects of prostaglandin (PG) E1, and I2 analogs (OP-41483 and OP-2507) on the Superoxide generation of human neutrophil NADPH oxidase (EC 1.6.99.6) in both whole-cell and cell-free systems were investigated.</li><li>2.2. In a whole-cell system, OP-2507 inhibited the Superoxide generation by neutrophils exposed to phorbol myristate acetate concentration-dependently through its superoxide-scavenging action.</li><li>3.3. The concentration of the drug required for 50% inhibition of the oxidase (IC50) was 21 μM.</li><li>4.4. In a cell-free system, however, the drug in concentrations of < 100 μM did not inhibit the activation of NADPH oxidase by sodium dodecyl sulfate because of its inactivation by the detergent.</li><li>5.5. Although PGE1 and OP-41483 did not inhibit the Superoxide production by stimulated neutrophils in a whole-cell system, they both inhibited the activation of NADPH oxidase in a cell-free system concentration-dependently, with IC50 values of 44 and 170 μM, respectively.</li><li>6.6. In addition, in the cell-free system, the Km value for NADPH of the oxidase was unchanged by PGE1.</li><li>7.7. The results suggest that the PGI2 analog, OP-2507, is a possible superoxide-scavenger and that PGE1 inhibits the NADPH oxidase activation by sodium dodecyl sulfate in a cell-free system concentration-dependently.</li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 8","pages":"Pages 1003-1008"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90071-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91656519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Interactions between adenosine A1- and histamine H1-receptors 腺苷A1和组胺h1受体之间的相互作用

International Journal of Biochemistry Pub Date : 1994-08-01 DOI: 10.1016/0020-711X(94)90066-3

John M. Dickenson, Stephen J. Hill

{"title":"Interactions between adenosine A1- and histamine H1-receptors","authors":"John M. Dickenson, Stephen J. Hill","doi":"10.1016/0020-711X(94)90066-3","DOIUrl":"10.1016/0020-711X(94)90066-3","url":null,"abstract":"<div><ul><li>1.1. The interactions or “cross-talk” between adenosine A1-receptors and receptors coupled to phospholipase C (leading to the hydrolysis of inositol phospholipids) have been well documented in the literature. For example, activating the A1-receptor selectively potentiates the histamine H1-receptor stimulated hydrolysis of inositol phospholipids in guinea-pig cerebral slices. In contrast, when the adenosine receptor is activated in the cerebral cortex of mouse or man the histamine response is selectively inhibited.</li><li>2.2. Our studies have focused on the smooth muscle cell line, DDT1MF-2, derived from hamster vas deferens. These cells express A1-receptors which, in addition to the expected negative coupling to adenylate cyclase, also stimulate inositol phospholipid hydrolysis and Ca2+ mobilization. These A1-receptors also potentiate histamine H1-receptor responses, i.e. inositol phospholipid hydrolysis and Ca2+ mobilization.</li><li>3.3. The mechanism(s) underlying the potentiation or inhibition of histamine H1-receptor responses by the adenosine A1-receptor remain to be unravelled. One mechanism may involve intracellular “cross-talk” at the G-protein level. This review will discuss how βγ subunits from G1 proteins could be involved in augmenting responses to calcium mobilizing receptors.</li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 8","pages":"Pages 959-969"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90066-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19081571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36

Quantitative assessment of the association of the α-I fragment of spectrin with oligomers of intact spectrin spectrin α- 1片段与完整spectrin低聚物关联的定量评价

International Journal of Biochemistry Pub Date : 1994-08-01 DOI: 10.1016/0020-711X(94)90067-1

Nerida Cole, Gregory B. Ralston

{"title":"Quantitative assessment of the association of the α-I fragment of spectrin with oligomers of intact spectrin","authors":"Nerida Cole, Gregory B. Ralston","doi":"10.1016/0020-711X(94)90067-1","DOIUrl":"10.1016/0020-711X(94)90067-1","url":null,"abstract":"<div><ul><li>1.1. The α-I fragment of human spectrin that carries the binding site on the α-chain of spectrin for the β -chain has been purified from limited trypsin digests of spectrin by means of FPLC.</li><li>2.2. The self-association of spectrin and the binding of the α-I fragment to spectrin heterodimers and to tetramers have been quantified through the use of gel electrophoresis, staining with Coomassie Blue, and quantification of the bound dye following elution with pyridine.</li><li>3.3. The parameters of self-association were found to be consistent with those estimated from sedimentation equilibrium analysis.</li><li>4.4. The data were consistent with a model in which both self-association and the binding of the α-I fragment are considered to occur through an intermediate in which the α-β interface is initially dissociated. The α-β interface in the heterodimer was found to be less stable than that of higher oligomers by approx. 3 kJ/mol.</li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 8","pages":"Pages 971-976"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90067-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19081572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Phosphoinositide hydrolysis in Ki-ras-transformed fibroblasts stimulated by platelet-derived growth factor and bradykinin 血小板源性生长因子和缓激素刺激ki -ras转化成纤维细胞的磷酸肌肽水解

International Journal of Biochemistry Pub Date : 1994-08-01 DOI: 10.1016/0020-711X(94)90077-9

Takahiko Kumada , Yoshiko Banno , Hideo Miyata , Yoshinori Nozawa

{"title":"Phosphoinositide hydrolysis in Ki-ras-transformed fibroblasts stimulated by platelet-derived growth factor and bradykinin","authors":"Takahiko Kumada , Yoshiko Banno , Hideo Miyata , Yoshinori Nozawa","doi":"10.1016/0020-711X(94)90077-9","DOIUrl":"10.1016/0020-711X(94)90077-9","url":null,"abstract":"<div><ul><li>1.1. Signal transduction in response to platelet-derived growth factor (PDGF)-BB and bradykinin (BK) have been examined by measuring inositol polyphosphate formation in NIH3T3 fibrobalst and v-Ki-ras -transformed NIH3T3 fibroblast (DT).</li><li>2.2. The PDGF-induced inositol polyphosphate formation in NIH3T3 was greater than that in DT cells, in which autophosphorylation of PDGF receptor and tyrosine phosphorylation of phospholipase C (PLC)-γ 1 were suppressed when examined by immunoblotting with anti-phosphotyrosine antibody.</li><li>3.3. On the other hand, BK-stimulation produced a much higher level of inositol polyphosphate in DT cells which have a greater number of BK receptors.</li><li>4.4. These results indicate that in Ki-ras transformed cells the decrease (caused by PDGF) and the increase (caused by BK) in phosphoinositide hydrolysis are due to the defective autophosphorylation of PDGF receptors leading to a reduction in PLC-γ 1 tyrosine phosphorylation and the overexpression of BK receptors, respectively.</li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 8","pages":"Pages 1049-1054"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90077-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19081681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Methylglyoxal and cell viability 甲基乙二醛和细胞活力

International Journal of Biochemistry Pub Date : 1994-08-01 DOI: 10.1016/0020-711X(94)90069-8

László Braun, Tamás Garzó, Pál Riba , József Mandl, Miklós Péter Kalapos

引用次数: 13

Stimulatory effects of lung cytochrome b5 on benzphetamine N-demethylation in a reconstituted system containing lung cytochrome P450 LgM2 肺细胞色素b5在含肺细胞色素P450 LgM2重组体系中对苯丙胺n -去甲基化的刺激作用

International Journal of Biochemistry Pub Date : 1994-08-01 DOI: 10.1016/0020-711X(94)90075-2

Emel Arinç, Roya P.K. Pasha , Orhan Adali, Nilay Başaran

{"title":"Stimulatory effects of lung cytochrome b5 on benzphetamine N-demethylation in a reconstituted system containing lung cytochrome P450 LgM2","authors":"Emel Arinç, Roya P.K. Pasha , Orhan Adali, Nilay Başaran","doi":"10.1016/0020-711X(94)90075-2","DOIUrl":"10.1016/0020-711X(94)90075-2","url":null,"abstract":"<div><ul><li>1.1. Cytochrome b5 was partially purified from sheep lung microsomes in the presence of detergents Emuigen 913 and cholate by three consecutive DEAE-cellulose and Sephadex G-100 gel filtration chromatographies.</li><li>2.2. The specific content ofcytochrome b5 was 16.5 nmol/mg protein and purified cytochrome b5 fractions were free of cytochrome P450, NADPH-cytochrome P450 reductase and NADH-cytochrome b5 reductase activities.</li><li>3.3. The influences of increasing concentrations of lung cytochrome b5 on benzphetamine N-demethylation reactions were examined in four different reconstitution systems containing lung cytochrome P 450 LgM2, lung cytochrome P450 reductase and lipid. In each system concentration of reductase was doubled with respect to former system.</li><li>4.4. In all systems cytochrome b 5 stimulated benzphetamine Ndemethylase activity especially when cytochrome b5 was present at 0.5:1 molar ratio with respect to cytochrome /P450 LgM2.</li><li>5.5. Besides, the greatest fold of increase in benzphetamine N-demethylation activity due to addition of cytochrome b5 was observed in System 1 with the lowest concentration of reductase.</li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 8","pages":"Pages 1033-1042"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90075-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19081679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Detection of deoxyribonucleases I and II (dnases I and II) activities in reproductive organs of male rabbits 雄性家兔生殖器官脱氧核糖核酸酶I和II (dna酶I和II)活性的检测

International Journal of Biochemistry Pub Date : 1994-08-01 DOI: 10.1016/0020-711X(94)90074-4

H. Takeshita, T. Yasuda, D. Nadano, E. Tenjo, K. Sawazaki, R. Iida, K. Kishi

引用次数: 28

Purification of glycosylphosphatidylinositol-anchoring aminopeptidase in from the plasma membrane of larval midgut epithelial cells of the silkworm, Bombyx mori 家蚕幼虫中肠上皮细胞质膜中糖基磷脂酰肌醇锚定氨基肽酶的纯化

International Journal of Biochemistry Pub Date : 1994-08-01 DOI: 10.1016/0020-711X(94)90068-X

Masahiro Tomita , Hiroshi Obara , Yoshiki Takesue , Hiro-Omi Tamura , Shigetoshi Miyajima , Ryo Taguchi , Hiroh Ikezawa

{"title":"Purification of glycosylphosphatidylinositol-anchoring aminopeptidase in from the plasma membrane of larval midgut epithelial cells of the silkworm, Bombyx mori","authors":"Masahiro Tomita , Hiroshi Obara , Yoshiki Takesue , Hiro-Omi Tamura , Shigetoshi Miyajima , Ryo Taguchi , Hiroh Ikezawa","doi":"10.1016/0020-711X(94)90068-X","DOIUrl":"10.1016/0020-711X(94)90068-X","url":null,"abstract":"<div><ul><li>1.1. Aminopeptidase N was selectively released from larval midgut of silkworm, Bombyx mori, by phosphatidylinositol-specific phospholipase C, and purified to a homogeneous state by ion exchange, gel filtration. Con A-Sepharose and 4-aminobenzyl phosphonic acid-agarose column chromatographies.</li><li>2.2. The purified aminopeptidase N preparation showed 190.8 U/mg of specific activity. Its molecular weight was estimated to be around 100 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.</li><li>3.3. Purified aminopeptidase N molecule preferentially hydrolyzed Leu-, Ala- and Met-p-nitroanilide as substrates. Especially, Leu-p-nitroanilide proved to be the best substrate for aminopeptidase N from larval midgut of silkworm.</li><li>4.4. By treatment with phosphatidylinositol-specific phospholipase C, two other hydrolases, alkaline phosphatase and alkaline phosphodiesterase I, were also solubilized from silkworm midgut.</li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 8","pages":"Pages 977-986"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90068-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84982884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Purification and principal properties of the casein kinase ii purified from the yeast Yarrowia lipolytica 脂肪化耶氏酵母酪蛋白激酶ii的纯化及其主要性质

International Journal of Biochemistry Pub Date : 1994-08-01 DOI: 10.1016/0020-711X(94)90073-6

Thierry Chardot, Jean-Claude Meunier

{"title":"Purification and principal properties of the casein kinase ii purified from the yeast Yarrowia lipolytica","authors":"Thierry Chardot, Jean-Claude Meunier","doi":"10.1016/0020-711X(94)90073-6","DOIUrl":"https://doi.org/10.1016/0020-711X(94)90073-6","url":null,"abstract":"<div><ul><li>1.1. A protein kinase from the lipolytic yeast Yarrowia lipolytica has been purified to nearby homogeneity.</li><li>2.2. The enzyme is able to phosphorylate casein and soybean purified storage proteins (β-conglycinin and glycinin) in the absence of cAMP.</li><li>3.3. Its activity and autophosphorylation are inhibited by very low concentrations of heparin.</li><li>4.4. Its native structure which is a tetramer of 170 kDa composed of two kinds of subunits, α and β (42 and 35 kDa, respectively), β being autophosphorylated. Through ordered aggregation phenomena 1800 and 580 kDa species can also be observed.</li><li>5.5. This enzyme belongs to the casein kinase II family.</li><li>6.6. We describe the use of a protein kinase to phosphorylate easily available reserve proteins. This system is a promising tool to study the specificity of the enzyme, and to find out the link between the phosphorylation, conformational changes, and functional properties.</li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 8","pages":"Pages 1017-1024"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90073-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90008337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Diacylglycerol and alkylacylglycerol stimulate ram sperm phospholipase A2 二酰基甘油和烷基酰甘油刺激公羊精子磷脂酶A2

International Journal of Biochemistry Pub Date : 1994-07-01 DOI: 10.1016/0020-711X(94)90089-2

E.R.S. Roldaim , R. Martinez-Dalmau , F. Mollinedo

{"title":"Diacylglycerol and alkylacylglycerol stimulate ram sperm phospholipase A2","authors":"E.R.S. Roldaim , R. Martinez-Dalmau , F. Mollinedo","doi":"10.1016/0020-711X(94)90089-2","DOIUrl":"10.1016/0020-711X(94)90089-2","url":null,"abstract":"<div><ul><li>1.1. We have investigated the susceptibility of ram sperm phospholipase A2 (PLA2) to stimulation by diacyi- and alkylacylglycerols and by monoacyi- and monoalkylglycerols.</li><li>2.2. PLA2 activity in sonicates from ram spermatozoa was enhanced when l-stearoyl-2-arachidonoyl-sn-glycerol, the diacylglycerol usually generated by polyphosphoinositide breakdown, was added to a radioactive phosphatidylcholine substrate; the effect was time- and Ca2+-dependent.</li><li>3.3. Both diacyi- and alkylacylglycerol considerably enhanced PLA2 activity; 1-O-hexadecyl-2-O-methyl-rac-glycerol, however, only showed slight stimulatory ability.</li><li>4.4. The monoradylglycerols 1-monohexadecanoyl-rac-glycerol, 2-monohexadecanoylglycerol, and 1-O-hexadecyl-.sn-glycerol had very little effect on the enzyme's activity.</li><li>5.5. Exposure of spermatozoa to 1-oleoyl-2-acetyl-.sn-glycerol (OAG) or 1-O-hexadecyl-2-acetyl-rac-glycerol (1-O-C16/2-C2), when cells were stimulated with the ionophore A23187 and Ca2+, resulted in higher PLA2 activity in sperm sonicates. Furthermore, parallel experiments showed that exocytosis was enhanced if spermatozoa were treated with A23187/Ca2+ and either OAG or 1-O-C16/2-C2. Since both diacyi- and alkylacylglycerols increased PLAi activity and exocytosis, stimulation of PLA2 activity by these diglycerides may take place independently from protein kinase C activation.</li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 7","pages":"Pages 951-958"},"PeriodicalIF":0.0,"publicationDate":"1994-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90089-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19056588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13