Emel Arinç, Roya P.K. Pasha , Orhan Adali, Nilay Başaran
{"title":"肺细胞色素b5在含肺细胞色素P450 LgM2重组体系中对苯丙胺n -去甲基化的刺激作用","authors":"Emel Arinç, Roya P.K. Pasha , Orhan Adali, Nilay Başaran","doi":"10.1016/0020-711X(94)90075-2","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Cytochrome <em>b</em>5 was partially purified from sheep lung microsomes in the presence of detergents Emuigen 913 and cholate by three consecutive DEAE-cellulose and Sephadex G-100 gel filtration chromatographies.</p></span></li><li><span>2.</span><span><p>2. The specific content ofcytochrome <em>b</em>5 was 16.5 nmol/mg protein and purified cytochrome <em>b</em>5 fractions were free of cytochrome <em>P</em>450, NADPH-cytochrome <em>P</em>450 reductase and NADH-cytochrome <em>b</em>5 reductase activities.</p></span></li><li><span>3.</span><span><p>3. The influences of increasing concentrations of lung cytochrome <em>b</em>5 on benzphetamine <em>N</em>-demethylation reactions were examined in four different reconstitution systems containing lung cytochrome <em>P</em> 450 LgM2, lung cytochrome P450 reductase and lipid. In each system concentration of reductase was doubled with respect to former system.</p></span></li><li><span>4.</span><span><p>4. In all systems cytochrome <em>b</em> 5 stimulated benzphetamine <em>N</em>demethylase activity especially when cytochrome <em>b</em>5 was present at 0.5:1 molar ratio with respect to cytochrome /<em>P</em>450 LgM2.</p></span></li><li><span>5.</span><span><p>5. Besides, the greatest fold of increase in benzphetamine <em>N</em>-demethylation activity due to addition of cytochrome <em>b</em>5 was observed in System 1 with the lowest concentration of reductase.</p></span></li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 8","pages":"Pages 1033-1042"},"PeriodicalIF":0.0000,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90075-2","citationCount":"9","resultStr":"{\"title\":\"Stimulatory effects of lung cytochrome b5 on benzphetamine N-demethylation in a reconstituted system containing lung cytochrome P450 LgM2\",\"authors\":\"Emel Arinç, Roya P.K. Pasha , Orhan Adali, Nilay Başaran\",\"doi\":\"10.1016/0020-711X(94)90075-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. Cytochrome <em>b</em>5 was partially purified from sheep lung microsomes in the presence of detergents Emuigen 913 and cholate by three consecutive DEAE-cellulose and Sephadex G-100 gel filtration chromatographies.</p></span></li><li><span>2.</span><span><p>2. The specific content ofcytochrome <em>b</em>5 was 16.5 nmol/mg protein and purified cytochrome <em>b</em>5 fractions were free of cytochrome <em>P</em>450, NADPH-cytochrome <em>P</em>450 reductase and NADH-cytochrome <em>b</em>5 reductase activities.</p></span></li><li><span>3.</span><span><p>3. The influences of increasing concentrations of lung cytochrome <em>b</em>5 on benzphetamine <em>N</em>-demethylation reactions were examined in four different reconstitution systems containing lung cytochrome <em>P</em> 450 LgM2, lung cytochrome P450 reductase and lipid. In each system concentration of reductase was doubled with respect to former system.</p></span></li><li><span>4.</span><span><p>4. In all systems cytochrome <em>b</em> 5 stimulated benzphetamine <em>N</em>demethylase activity especially when cytochrome <em>b</em>5 was present at 0.5:1 molar ratio with respect to cytochrome /<em>P</em>450 LgM2.</p></span></li><li><span>5.</span><span><p>5. Besides, the greatest fold of increase in benzphetamine <em>N</em>-demethylation activity due to addition of cytochrome <em>b</em>5 was observed in System 1 with the lowest concentration of reductase.</p></span></li></ul></div>\",\"PeriodicalId\":13733,\"journal\":{\"name\":\"International Journal of Biochemistry\",\"volume\":\"26 8\",\"pages\":\"Pages 1033-1042\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1994-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0020-711X(94)90075-2\",\"citationCount\":\"9\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Biochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0020711X94900752\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0020711X94900752","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Stimulatory effects of lung cytochrome b5 on benzphetamine N-demethylation in a reconstituted system containing lung cytochrome P450 LgM2
1.
1. Cytochrome b5 was partially purified from sheep lung microsomes in the presence of detergents Emuigen 913 and cholate by three consecutive DEAE-cellulose and Sephadex G-100 gel filtration chromatographies.
2.
2. The specific content ofcytochrome b5 was 16.5 nmol/mg protein and purified cytochrome b5 fractions were free of cytochrome P450, NADPH-cytochrome P450 reductase and NADH-cytochrome b5 reductase activities.
3.
3. The influences of increasing concentrations of lung cytochrome b5 on benzphetamine N-demethylation reactions were examined in four different reconstitution systems containing lung cytochrome P 450 LgM2, lung cytochrome P450 reductase and lipid. In each system concentration of reductase was doubled with respect to former system.
4.
4. In all systems cytochrome b 5 stimulated benzphetamine Ndemethylase activity especially when cytochrome b5 was present at 0.5:1 molar ratio with respect to cytochrome /P450 LgM2.
5.
5. Besides, the greatest fold of increase in benzphetamine N-demethylation activity due to addition of cytochrome b5 was observed in System 1 with the lowest concentration of reductase.
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