{"title":"前列腺素E1和前列腺素类似物影响人中性粒细胞nadph氧化酶系统产生超氧化物","authors":"Shigenobu Umeki","doi":"10.1016/0020-711X(94)90071-X","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. The effects of prostaglandin (PG) E<sub>1</sub>, and I<sub>2</sub> analogs (OP-41483 and OP-2507) on the Superoxide generation of human neutrophil NADPH oxidase (EC 1.6.99.6) in both whole-cell and cell-free systems were investigated.</p></span></li><li><span>2.</span><span><p>2. In a whole-cell system, OP-2507 inhibited the Superoxide generation by neutrophils exposed to phorbol myristate acetate concentration-dependently through its superoxide-scavenging action.</p></span></li><li><span>3.</span><span><p>3. The concentration of the drug required for 50% inhibition of the oxidase (IC<sub>50</sub>) was 21 μM.</p></span></li><li><span>4.</span><span><p>4. In a cell-free system, however, the drug in concentrations of < 100 μM did not inhibit the activation of NADPH oxidase by sodium dodecyl sulfate because of its inactivation by the detergent.</p></span></li><li><span>5.</span><span><p>5. Although PGE<sub>1</sub> and OP-41483 did not inhibit the Superoxide production by stimulated neutrophils in a whole-cell system, they both inhibited the activation of NADPH oxidase in a cell-free system concentration-dependently, with IC<sub>50</sub> values of 44 and 170 μM, respectively.</p></span></li><li><span>6.</span><span><p>6. In addition, in the cell-free system, the <em>K</em><sub><em>m</em></sub> value for NADPH of the oxidase was unchanged by PGE<sub>1</sub>.</p></span></li><li><span>7.</span><span><p>7. The results suggest that the PGI<sub>2</sub> analog, OP-2507, is a possible superoxide-scavenger and that PGE<sub>1</sub> inhibits the NADPH oxidase activation by sodium dodecyl sulfate in a cell-free system concentration-dependently.</p></span></li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 8","pages":"Pages 1003-1008"},"PeriodicalIF":0.0000,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90071-X","citationCount":"7","resultStr":"{\"title\":\"Prostaglandin E1 and analogs of prostacyclin influencing superoxide production by the human neutrophil nadph oxidase system\",\"authors\":\"Shigenobu Umeki\",\"doi\":\"10.1016/0020-711X(94)90071-X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. The effects of prostaglandin (PG) E<sub>1</sub>, and I<sub>2</sub> analogs (OP-41483 and OP-2507) on the Superoxide generation of human neutrophil NADPH oxidase (EC 1.6.99.6) in both whole-cell and cell-free systems were investigated.</p></span></li><li><span>2.</span><span><p>2. In a whole-cell system, OP-2507 inhibited the Superoxide generation by neutrophils exposed to phorbol myristate acetate concentration-dependently through its superoxide-scavenging action.</p></span></li><li><span>3.</span><span><p>3. The concentration of the drug required for 50% inhibition of the oxidase (IC<sub>50</sub>) was 21 μM.</p></span></li><li><span>4.</span><span><p>4. In a cell-free system, however, the drug in concentrations of < 100 μM did not inhibit the activation of NADPH oxidase by sodium dodecyl sulfate because of its inactivation by the detergent.</p></span></li><li><span>5.</span><span><p>5. Although PGE<sub>1</sub> and OP-41483 did not inhibit the Superoxide production by stimulated neutrophils in a whole-cell system, they both inhibited the activation of NADPH oxidase in a cell-free system concentration-dependently, with IC<sub>50</sub> values of 44 and 170 μM, respectively.</p></span></li><li><span>6.</span><span><p>6. In addition, in the cell-free system, the <em>K</em><sub><em>m</em></sub> value for NADPH of the oxidase was unchanged by PGE<sub>1</sub>.</p></span></li><li><span>7.</span><span><p>7. The results suggest that the PGI<sub>2</sub> analog, OP-2507, is a possible superoxide-scavenger and that PGE<sub>1</sub> inhibits the NADPH oxidase activation by sodium dodecyl sulfate in a cell-free system concentration-dependently.</p></span></li></ul></div>\",\"PeriodicalId\":13733,\"journal\":{\"name\":\"International Journal of Biochemistry\",\"volume\":\"26 8\",\"pages\":\"Pages 1003-1008\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1994-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0020-711X(94)90071-X\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Biochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0020711X9490071X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0020711X9490071X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Prostaglandin E1 and analogs of prostacyclin influencing superoxide production by the human neutrophil nadph oxidase system
1.
1. The effects of prostaglandin (PG) E1, and I2 analogs (OP-41483 and OP-2507) on the Superoxide generation of human neutrophil NADPH oxidase (EC 1.6.99.6) in both whole-cell and cell-free systems were investigated.
2.
2. In a whole-cell system, OP-2507 inhibited the Superoxide generation by neutrophils exposed to phorbol myristate acetate concentration-dependently through its superoxide-scavenging action.
3.
3. The concentration of the drug required for 50% inhibition of the oxidase (IC50) was 21 μM.
4.
4. In a cell-free system, however, the drug in concentrations of < 100 μM did not inhibit the activation of NADPH oxidase by sodium dodecyl sulfate because of its inactivation by the detergent.
5.
5. Although PGE1 and OP-41483 did not inhibit the Superoxide production by stimulated neutrophils in a whole-cell system, they both inhibited the activation of NADPH oxidase in a cell-free system concentration-dependently, with IC50 values of 44 and 170 μM, respectively.
6.
6. In addition, in the cell-free system, the Km value for NADPH of the oxidase was unchanged by PGE1.
7.
7. The results suggest that the PGI2 analog, OP-2507, is a possible superoxide-scavenger and that PGE1 inhibits the NADPH oxidase activation by sodium dodecyl sulfate in a cell-free system concentration-dependently.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
