Detection of deoxyribonucleases I and II (dnases I and II) activities in reproductive organs of male rabbits

H. Takeshita, T. Yasuda, D. Nadano, E. Tenjo, K. Sawazaki, R. Iida, K. Kishi
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引用次数: 28

Abstract

  • 1.

    1. Deoxyribonucleases (DNases) I and II activities in 13 different organs and body fluids from healthy male rabbits were measured using the single radial enzyme diffusion method.

  • 2.

    2. We now show that testis, epididymis, ampulla, seminal vesicle, vesicular gland, prostate, and semen have both of the DNases I and II activities, whereas spermatozoa do not.

  • 3.

    3. DNase I activities were highest in epididymis and seminal vesicle, whereas DNase II activities were highest in epididymis and prostate among the reproductive organs.

  • 4.

    4. The presence of these two enzyme activities outside the digestive system suggests that they have another biological function in addition to their digestive roles.

雄性家兔生殖器官脱氧核糖核酸酶I和II (dna酶I和II)活性的检测
1.1. 采用单径向酶扩散法测定了健康雄性家兔13种不同器官和体液中脱氧核糖核酸酶(dnase) I和II的活性。我们现在表明,睾丸、附睾、壶腹、精囊、囊泡腺、前列腺和精液都具有dnase I和dnase II的活性,而精子则没有。在生殖器官中,DNase I活性以附睾和精囊最高,而DNase II活性以附睾和前列腺最高。这两种酶在消化系统外的活性表明,除了消化作用外,它们还有另一种生物学功能。
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