International Blood Research & Reviews最新文献

{"title":"Determining the Genotype of the RH Blood Group System in Maltese Blood Donors","authors":"Antonella Giordmaina Powney, Joseph Borg, Jesmond Debono","doi":"10.9734/ibrr/2023/v14i4325","DOIUrl":"https://doi.org/10.9734/ibrr/2023/v14i4325","url":null,"abstract":"Aims: The Rh blood group system is a complex protein based system having the D, C, c, E and e as the most immunogenic antigens. This blood group system has a vital role in transfusion medicine thus by DNA typing, important medical issues in transfusion practice can be resolved when serological techniques fail. The aim of the study was to determine the frequency of the RH genotype in the Maltese blood donors using molecular techniques.
 Methodology: 797 blood donor samples were enrolled in this study. An allele-specific polymerase chain reaction (AS-PCR) method was used to determine the presence of RHD, RHCE*E and RHCE*e, while multiplex PCR was used to test for RHCE*C/c. 
 Results: Out of 797 samples studied, the most common allele was RHCE*e with a percentage of 98. The most common genotype in RhD positive samples was DCcee and in RhD negative samples, the most common was dccee. The most frequent haplotype was DCe.
 Conclusion: Like in previous studies, this research also concludes that the distribution of the RH genotype varies in different geographical areas. Further studies working with larger sample size and better techniques would share better light on the molecular function of these loci.","PeriodicalId":13659,"journal":{"name":"International Blood Research & Reviews","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136211454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic Review of Genetic-Related Risk Factor and Inhibitor Epidemiology in People with Severe Hemophilia a from Africa: A 2023 Update","authors":"Tatiana Baglo, Alban Zohoun, Simon Azonbakin, Bienvenu Houssou, Romaric Massi, Charlotte Orou Guiwa, Ludovic Anani, Dorothée Kindé Gazard, Awa Omar Touré Fall","doi":"10.9734/ibrr/2023/v14i4324","DOIUrl":"https://doi.org/10.9734/ibrr/2023/v14i4324","url":null,"abstract":"Background and Aims: Prevalence of factor VIII inhibitors in patients with hemophilia A varies from study to study, ranging from 15% to 30%. The important risk of inhibitor development is factor VIII mutation responsible for hemophilia A. Few studies have reported factor VIII mutations in Africa. The aim of this study was to review on FVIII gène mutations of severe hemophilia A in Africa and those associated with inhibitor development.
 Study Design and Methodology: A systematic review was carried out using the electronic databases Pubmed, Science Direct, Index Medicus Global and African Journals online and the key words \"hemophilia A\", \"inhibitor\", \"genetic\" and \"Africa\". Studies written in French or English on the African continent and published between 2012 and 2023 were included. Publications relating to acquired hemophilia and duplicates were excluded. In the end, 17 articles were selected.
 Results: The factor VIII mutations involved in severe hemophilia A in Africa are variable, consisting of intron 22 inversion, large or point deletions, nonsense and missense mutations and splicing abnormalities. Among the latter, numerous previously unrecorded mutations have been identified, and a single case of intron 1 inversion has been found in Algeria. Prevalence of factor VIII inhibitors in severe hemophilia A in Africa varies between 7,8% and 30%. Genetic abnormalities associated with inhibitors include intron 22 inversion, large deletions such as exon 1-13 deletion, nonsense mutations and c.1010-2A>G mutation.
 Conclusion: A better knowledge of the factor VIII mutations involved in severe hemophilia A in Africa will help improve patient management.","PeriodicalId":13659,"journal":{"name":"International Blood Research & Reviews","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135142092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood Transfusion Request and Utilization: The Trend in a Tertiary Health Care Centre in North Central Nigeria","authors":"None Jatau E. D., None Ma’an V. T., None Ofakunrin A. O. D., None Ntuhun B. D., None Damulak O. D.","doi":"10.9734/ibrr/2023/v14i4323","DOIUrl":"https://doi.org/10.9734/ibrr/2023/v14i4323","url":null,"abstract":"Background: An effective blood transfusion service is anchored on an adequate supply of safe blood and its components as well as its accessibility and appropriate clinical use. There is a contrast in blood supplies and utilization between the high, middle and low-income countries necessitating the formulation of appropriate modalities to ensure judicious use of blood while minimizing wastage.
 Aims: This study was aimed at assessing the blood transfusion request trend in our institution and its utilization with the intent of curtailing the wastage of safe blood for transfusion.
 Study Design: It is a retrospective study.
 Place and Duration of Study: Jos University Teaching Hospital Blood Bank from January 2022 to December 2022.
 Methodology: Appropriately filled blood transfusion request forms of the Jos University Teaching Hospital compared with the blood bank In-house records from January 2022 to December 2022 were reviewed. Request dates, requesting Department, Unit or Specialty, indications for request, Number of units requested, and number eventually utilized were used to determine blood transfusion indices which are indicative of efficient and significant blood use.
 Results: Eight thousand, five hundred forty-eight (8,548) blood transfusion request forms from the blood bank and patients' folders were reviewed and compared with the Blood Bank's in-house records. Requests for blood transfusion for over 460 indications were received from twenty-six (26) departments and units. There was a general Crossmatch: Transfusion ratio (C: T) of 1.7 with a transfusion index (TI) of 1.2 and transfusion probability (TP) of 48.0%.
 Conclusion: Transfusion requests were characterized by low utilization across departments and units. This calls for the establishment of a transfusion protocol for the determination of blood volume required for every indication so that this limited commodity will be made available and accessible when needed.","PeriodicalId":13659,"journal":{"name":"International Blood Research & Reviews","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134977171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction of Red Cell Indices and Blood Group in Diabetic Patients above 50 Years of Age","authors":"None Moore-Igwe Beatrice W., None Ken-Ezihuo Stella U., Baribefe Banavule Daniel Koate","doi":"10.9734/ibrr/2023/v14i4322","DOIUrl":"https://doi.org/10.9734/ibrr/2023/v14i4322","url":null,"abstract":"Introduction: A group of metabolic diseases with a hyperglycemic phenotype known as diabetes mellitus impact both industrialized and developing nations. It is brought on by deviations in insulin synthesis, action, or both.&#x0D; Research Objective: This study seeks to associate the interaction of red cell indices and blood group in diabetic patients above 50 years of age. This is a case control study in which a convenient sample size of 100 individuals were recruited from a consenting population at a private hospital in Port Harcourt, Rivers State, 50 of whom were diabetic, and the remaining 50 were not.&#x0D; Methods: Using the automated 3-part differential hematology analyzer Sysmex KX-21N, a Full blood count (FBC) and differential was obtained using (Sysmex Corporation Kobe, Japan) Standardization, instrument calibration, and analysis of sample was achieved in line with accordance with the manufacturer's manual to determine the red cell indices and grouping of cells using the tile approach to determine the ABO Blood Groups. Blood samples were taken from the veins using a vacutainer tube and needle according to Chesbrough's instructions, and then put in EDTA anticoagulated bottles with clear labelling. These samples were transported right away using the triple packing procedure to the lab and stored until testing at 4°C. Inferential statistics utilizing the students' statistical t-test were employed to compare the results.&#x0D; Results: Blood group O, with a total of 25 (50%), had the highest prevalence of diabetes mellitus, followed by blood group A with a total of 12 subjects (24%) and blood group B with a total of 8 subjects (16%) and blood group AB came in last with a total of 5 participants (10%), respectively. In this study, blood group O predominated over blood groups A and B (47% against 25% versus 17%, respectively). The least common blood type in this study was AB (11%). When the Packed Cell Volume, Haemoglobin, Red Blood Cell, Mean Cell volume, Mean Cell Haemoglobin, Mean Cell Haemoglobin Concentration, and Red cell Distribution Width were examined between diabetic and non-diabetic participants, no statistically significant (p<0.05) difference was found.&#x0D; Conclusion: The findings of this study suggest that blood types O and AB are more prevalent in diabetics. We can deduce that a person's blood type may be a risk factor for developing this condition and may be useful in diagnosing it.","PeriodicalId":13659,"journal":{"name":"International Blood Research & Reviews","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135199773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of Severe Acute Respiratory Syndrome Corona Virus-2 (SARS-CoV-2) Immunoglobulins G and M among Frontline Health Workers in Eleme Local Government of Rivers State, Nigeria","authors":"Sotonye L. Gillis-Harry, Beauty E. Echonwere-Uwikor, Evelyn M. Eze","doi":"10.9734/ibrr/2023/v14i4320","DOIUrl":"https://doi.org/10.9734/ibrr/2023/v14i4320","url":null,"abstract":"Background: The fight against Corona Virus Disease (COVID-19) globally among front line health workers has been a challenging task, it encompasses working lengthy hours at the isolation Centre’s, intensive care units, emergency departments and molecular laboratories, and also been exposed to pathogens, fatigue, and psychological distress. This has led to high morbidity and mortality rate among front line health workers all over the globe.&#x0D; Aim: This study aimed at determining the distribution of SARS CoV -2 IgG and IgM among frontline health workers in Eleme Local Government of Rivers State, Nigeria.&#x0D; Materials and Methods: A total of 100 frontline Covid-19 health workers were recruited for this study comprising 48 males and 52 females with ages between 23 and 49 years and included janitors, hygienist, nurses, data Officers, logistics and ambulance drivers, physicians, and medical laboratory scientists, working at the molecular laboratories, sample collection booths and isolation center. The bio-data of the subjects were obtained using a well-structured questionnaire. Only subjects who gave informed consent were recruited for this study. 60ul of capillary blood was collected from each participant using an aseptic technique and immediately followed by the analysis of SAR-CoV-2 IgM and IgG using a lateral flow immunochromatographic assay technique.&#x0D; Results: Results from this study showed a total of forty-two (42) 42% subjects were reactive to IgG antibodies while fifty-eight (58) 58% subjects were non-reactive to IgG antibodies also a total of Twenty-One (21) 21% subject were reactive to IgM antibodies while a total of seventy-nine (79) 79% subjects were none reactive to IgM antibodies. A total of nine (9) subjects who were exposed to SAR-CoV-2 for more than one year were reactive to SAR-Cov-2 IgM and IgG antibodies, while a total of two (2) subjects who were exposed to SAR-CoV-2 for less than one year were reactive to SAR-Cov-2 IgM and IgG antibodies and the difference (p ₌ 0.013) was statistically significant. Also, a higher number of subjects within the 20-29 age bracket were reactive to SAR-CoV-2 IgM antibodies while subjects with in the age bracket of 30-39 were more reactive to IgG antibodies.&#x0D; Conclusion: This study reveals that serological testing is an ideal approach in assessing the proportion of frontline health workers who might have been exposed to SARS-CoV-2 as part of effort in combating COVID-19 disease globally.","PeriodicalId":13659,"journal":{"name":"International Blood Research & Reviews","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134913363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Umbilical Cord Blood Processing Techniques and Their Comparative Advantages: A Review","authors":"Eze Evelyn Mgbeoma, Christian Serekara Gideon","doi":"10.9734/ibrr/2023/v14i4321","DOIUrl":"https://doi.org/10.9734/ibrr/2023/v14i4321","url":null,"abstract":"Background: Umbilical Cord Blood (UCB) has steadily gained prominence in haematopoietic stem cell transplantation (HSCT). Despite UCB advantages, the main disadvantage of UCB in haematopoietic stem cell transplantation (HSCT) is its limited cell dose. Initially, UCB used to be processed and then made to undergo cryopreservation as whole cord blood banking leading to the problem of storing sufficiently large number of cryoprotected UCB units which requires vast amounts of costly storage space in liquid nitrogen. The sole purpose of processing is to concentrates the stem cells and reduce the volume for storage. Different UCB processing methods have been developed. &#x0D; Aim: This review is aimed at bringing together the literature on the different processing methods and highlighting the underlying principles of each method, the relative efficiency and advantages of the methods.&#x0D; Methodology: The work involved mainly the critical review of all available academic, professional and industry documents on cord blood processing. The relevant information was obtained from textbooks, academic journals, conference proceedings, the internet among others. The major UCB processing methods include Plasma Depletion, Density Gradient Centrifugation (DGC), Hetastarch, PrepaCyte-CB and Sepax. A study of the potential impact of Hetastarch and PrepaCyte processing methods on transplantation outcomes revealed no difference that was significant was observed between patients receiving cells after the processing regimens were compared.&#x0D; Results: A comparison of the engraftment time of PrepaCyte-CB with five other processing methods revealed a quicker engraftment time for PrepaCyte-CB processed cord blood units compared to other processing methods. PrepaCyte-CB also recovers significantly more viable stem cells than AutoXpress (AXP) and hydroxyethyl starch (HES) processing methods. Other workers demonstrated that Sepax depletion produces higher recovery of cells that are nucleated. The effect initial volume of cord blood had on the recovery of nucleated cells for the different method of processing were also compared. Recovery when using Sepax is reduced as the unit size processed increases. Hetastarch, which is a density gradient, and plasma depletion separation is also affected in like manner, however, processing done using PrepaCyte-CB was not affected by the initial volume of the collected unit. The advantage of Sepax is that it is fully automated and this allows for mass processing of samples, suitable for bigger cord blood banks. For erythrocyte removal, density gradient separation is a better method that is effective. PrepaCyte-CB is the second most efficient method for removing RBC. The result of Total Nucleated Cells (TNC) and Mono Nucleated Cells (MNC) recovery rate of Hespan and Sepax against AXP processing methods shows that both Hespan and Sepax reproducibly recover greater than 95% of the cord blood stem cells in a typical collection and result in ","PeriodicalId":13659,"journal":{"name":"International Blood Research & Reviews","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134913722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What a Clinical Hematologist should Know about B Cells?","authors":"S. Sharma","doi":"10.9734/ibrr/2022/v13i130166","DOIUrl":"https://doi.org/10.9734/ibrr/2022/v13i130166","url":null,"abstract":"B cells are very crucial part of our immune system. They protect us from various infections by secreting antigen-specific antibodies, which neutralize the infectious agents. It is important for a clinical hematologist to know about the B cell development, function and the diseases developing from the quantitative or qualitative defects in B cells. This simple and short review is focused on the basic understanding and clinical hematologist’s perspective of B cells.","PeriodicalId":13659,"journal":{"name":"International Blood Research & Reviews","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73580943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Coagulation and Haematological Parameters among Pregnant Women in Port Harcourt","authors":"B. Echonwere-uwikor, F. K. Uwikor, O. Chukuigwe-Igbere, P. H. Chukwu, Gift Worlu","doi":"10.9734/ibrr/2022/v13i130165","DOIUrl":"https://doi.org/10.9734/ibrr/2022/v13i130165","url":null,"abstract":"Background: Pregnancy is the fertilization and development of an embryo or fetus in a woman's uterus. It is a critical stage of development during which maternal nutrition can strongly influence obstetric and neonatal outcomes. The aim of this study was to determine the effect of pregnancy on some coagulation and haematological parameters of pregnant women residing in Port Harcourt, Nigeria. \u0000Method: This case-control study investigated 80 pregnant subjects and 20 non-pregnant controls. Haematological parameters were determined using a fully automated The SysmexXP-300, while the coagulation parameters (PT and INR) were determined with the automated method. \u0000Results: The mean PT (s), HB (g/dl), PCV (%), PLT (x109/L), RBC (mcL), LYMPH (%), BASO (%) and EOSIN (%) counts were significantly lower among the pregnant subjects (5.02±5.82, 11.00±1.13 g/dl and 33.81±3.89%, 189.6±52.93 × 109/L, 3.95±0.50, 43.93±10.10 %, 0.9385±1.08 % and 1.12±1.32 %) compared to the non-pregnant controls (1.68±2.37, 12.01±1.29 g/dl, 37.31±3.39 %, 235.6±72.37 × 109/L, 4.45±0.35, 43.24±9.06, 2.11±0.94 and 2.15±1.47) respectively. There were no significant differences in the INR, MONO (%), MPV (fl), MCV (L/C), and MCH (g/c) between the pregnant subjects (0.92±0.11, 5.41±2.59 %, 11.67±4.848, 84.26±3.77 and 28.06±3.54) and non-pregnant controls (0.88±0.11, 5.58±2.65, 11.14±5.45, 82.52±10.45 and 26.96±1.85). The NEUT (%), PDW (%) and WBC (x109/L) were significantly higher among the pregnant subjects (44.90±11.24 %, 13.26±2.56 % and 8.35±2.982 x109/L) compared to the non-pregnant controls (46.63±9.96 %, 10.78±1.80 % and 4.71±0.81 x109/L). \u0000Conclusion: This study has shown that pregnancy has a significant effect on some haematological and coagulation parameters of pregnant women in Port Harcourt. The result of this research work indicates the need to routinely monitor the complete blood count, thrombocytopenia and hyper-coagulative activity among pregnant women of African descendant.","PeriodicalId":13659,"journal":{"name":"International Blood Research & Reviews","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84756803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Some Haematological, Coagulation and Immune Parameters among Male Oil Refinery Workers in Port Harcourt, Nigeria","authors":"Ehiaghe-Shepherd, Bridget Obofoni, Christian, Serekara Gideon, Eze, Evelyn Mgbeoma","doi":"10.9734/ibrr/2021/v13i130164","DOIUrl":"https://doi.org/10.9734/ibrr/2021/v13i130164","url":null,"abstract":"Aim: The aim of this study was to assess the levels of some hematological, coagulation and immune parameters among male oil refinery workers in Port Harcourt, Nigeria. \u0000Study Design: This study is a cross-sectional study. \u0000Place and Duration of Study: Rivers State University Teaching Hospital (RSUTH), Port Harcourt, Shell Petroleum Development Company of Nigeria Limited and Modular Oil Refinery, Rivers State, between January 2021 and September 2021. \u0000Methodology: A total of one hundred (100) subjects (50 oil and gas workers as test subjects and 50 non-oil and gas workers as control subjects), were enrolled in the study. The convenient sampling technique was employed in the study. Venous blood samples were collected from all subjects and tested for Prothrombin Time (PT), Activated Partial Thromboplastin Time (APTT), Fibrinogen concentration, Full Blood Count (FBC) (Haemoglobin (Hb), Packed Cell Volume (PCV), Total White Blood Cell (WBC) count, Lymphocyte, Neutrophils, monocytes and Platelet Count), CD4 cell count, CD3 cell count and CD8 cell count. Data generated were analyzed statistically using Graph-Pad Prism, Version 8.0.2. \u0000Results: There was statistically significant decrease (P<.001) in Prothrombin time (PT) (11.55±0.73s), International Normalized Ratio (INR) (0.85±0.05), Fibrinogen (202.4±27.4mg/dl), Platelet count (185.6±37.1(103/μl)) and Neutrophils (46.6±6.4%) in oil refinery workers exposed to gas flare, while there was a statistical significant increase in APTT (31.8±4.15s), Hb (13.7±1.0g/dl), PCV (41.1±3.2%), Monocytes (8.4±3.0%) in subjects exposed to gas flare over control subjects with Prothrombin time(12.23±0.82s), INR(0.90±0.06), Fibrinogen(252.0±57.0mg/dl), platelet count(213.3±49.5(103/μl)) and Neutrophils(52.6±11.7%). Other parameters showed no statistical significant difference at P<.05 in both test and control subjects. Comparison of the mean ± standard deviation of the studied parameters in test subjects based on age using Analysis of Variance showed no statistically significant difference in all parameters at P<.05. Also, Comparison of the mean ± standard deviation of the studied parameters in test subjects based on duration of Exposure using Analysis of Variance showed a significant decrease in CD8 cells as the years of exposure increase (2-5years exposure = 865±319, 6-10years exposure = 579±288, 11-20 years exposure = 591± 286, F- Value = 3.869, P- Value = 0.0278). \u0000Conclusion: In conclusion, based on the findings, some haematogical, coagulation and immune parameters increased while others decreased in male oil refinery workers and duration of exposure can also be considered as a risk factor and age was considered not a risk factor as to cause any aberrations in the studied parameters.","PeriodicalId":13659,"journal":{"name":"International Blood Research & Reviews","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82125768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of FMS-Like Tyrosine Kinase 3 Mutation Frequency in Myelodysplastic Syndrome","authors":"Nesim Akın, A. Turgutkaya, S. I. Yavaşoğlu, Esra Örenlili Yaylagül, Celal Ülger, A. Bolaman, I. Yavaşoğlu","doi":"10.9734/ibrr/2021/v13i130163","DOIUrl":"https://doi.org/10.9734/ibrr/2021/v13i130163","url":null,"abstract":"Introduction: FMS-Like Tyrosine Kinase Class 3 (FLT3) mutations harbor poor prognosis, high relapse, and decreased overall survival in acute myeloblastic leukemia (AML). This mutation is also known to be demonstrated in myelodysplastic syndrome (MDS), chronic myelomonocytic leukemia and acute lymphoblastic leukemia. This study included 94 MDS-diagnosed patients and we tried to investigate FLT3 mutation frequency (as tyrosine kinase domain-TKD and internal tandem duplication-ITD). \u0000Materials and Methods: Polymerase chain reaction (PCR), restriction fragment length polymorphism, and agarose-gel electrophoresis methods were used to analyze the mutation. The blood samples were collected in K3-EDTA tubes, and total DNA was isolated using genomic DNA isolation kits (GeneMark, Cat No: DP023P). For the detection of FLT3-ITD mutation, PCR was performed to amplify a 330- base pair fragment of exons 11 and 12 of FLT3 using FAM (Carboxyfluorescein)-labeled ITD-11F and HEX (Hexachloro-Fluorescein )-labeled  ITD-12R primers in a thermal cycler (Eppendorf). Similarly, to detect D835 mutation, a 115- bp region of exon 17 of the FLT3 gene region was amplified using primers. \u0000Results: One patient was found FLT3-ITD positive (1.1%). The patient was 64-year-old and diagnosed with MDS-excess blast type 2 according to the World Health Organisation 2016 myeloid neoplasm classification. He transformed to AML within 19 months and subsequently died after 1 month. No patient with tyrosine kinase domain mutation was detected. \u0000Conclusion: FLT3 mutation is considered a significant parameter to define prognosis in AML. The routine workup of FLT3 screening and the potential of targeting FLT3 inhibition for high-risk MDS may be taken into consideration in the future.","PeriodicalId":13659,"journal":{"name":"International Blood Research & Reviews","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83911963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}