Insects最新文献

{"title":"Species Delimitation and Cryptic Diversity in <i>Rheotanytarsus</i> Thienemann & Bause, 1913 (Diptera: Chironomidae) Based on DNA Barcoding.","authors":"Yuan Yao, Jia-Yu Chen, Xiao-Ling Gong, Chen-Hong Li, Zheng Liu, Xiao-Long Lin","doi":"10.3390/insects16040370","DOIUrl":"https://doi.org/10.3390/insects16040370","url":null,"abstract":"<p><p>The genus <i>Rheotanytarsus</i> Thienemann & Bause, 1913 (Diptera: Chironomidae) currently includes more than 120 recognized species worldwide, but precise species-level identification based solely on morphology remains challenging. Pronounced morphological differences among life stages and the time-consuming inefficiency of rearing larvae further complicate life-stage matching in this genus. In this study, we assessed species diversity by integrating morphological examination and DNA barcoding, analyzing 911 DNA barcodes from newly collected samples and a public database. Based on these results, we further constructed a relatively complete life-history framework. Our results show that 911 <i>Rheotanytarsus</i> DNA barcodes belong to 69 putative species. The maximum intraspecific divergence reached 7.35% in <i>R. pentapoda</i>, and the average minimal interspecific distance was 11.44%. Substantial intraspecific divergence in certain species complexes suggests the presence of cryptic species. Therefore, to resolve these potential cryptic species issues, more extensive sampling and morphological examination of specimens from geographically distant regions-supplemented by nuclear and ecological data-are required.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12028281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing Electrostatic Forces: A Review of Bees as Bioindicators for Particulate Matter Detection.","authors":"Simone Meacci, Lorenzo Corsi, Eleonora Santecchia, Sara Ruschioni","doi":"10.3390/insects16040373","DOIUrl":"https://doi.org/10.3390/insects16040373","url":null,"abstract":"<p><p>Bees (Hymenoptera, Anthophila) are widely recognized for their essential ecological roles, including pollination and biodiversity maintenance. Recently, their ability to collect environmental particulate matter through electrostatic forces has been explored for biomonitoring purposes. This review integrates knowledge on electrostatic pollen adhesion with emerging insights into particulate matter adhesion to bees, emphasizing their potential as bioindicators. The mechanisms of electrostatic adhesion, influenced by factors such as the physicochemical properties of particulate matter and bee morphology, are discussed in detail. Additionally, the study evaluates the adhesion efficiency of pollutants, including heavy metals, microplastics, nanoplastics, pathogens, pesticides, radionuclides, and volatile organic compounds. This multidisciplinary approach underscores the role of bees in advancing environmental monitoring methodologies and offers innovative tools for assessing ecosystem health while addressing the drivers of bee decline.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12027818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of <i>Rachiplusia nu</i> and <i>Chrysodeixis includens</i> in <i>Bt</i> and Conventional Soybean Fields in Brazil.","authors":"Carolina T D Godói, Tamylin K Ishizuka, Guilherme A Gotardi, Natália R F Batista, Luiz H Marques, Antônio César S Santos, Mário H Dal Pogetto, Timothy Nowatzki, Amit Sethi, Mark L Dahmer","doi":"10.3390/insects16040365","DOIUrl":"https://doi.org/10.3390/insects16040365","url":null,"abstract":"<p><p><i>Chrysodeixis includens</i> (Walker, 1858) and <i>Rachiplusia nu</i> (Guenée, 1852) are important defoliating pests belonging to the Plusiinae subfamily in the Western hemisphere. <i>C. includens</i> is a major lepidopteran pest of soybean in the Americas, whereas, until 2021, <i>R. nu</i> was more restricted to the temperate regions of South America. Recently, reports of <i>R. nu</i> feeding on Cry1Ac soybean and occurring in tropical regions of Brazil have raised questions regarding the distribution of this species. The morphological similarity of the larvae from the two species makes it difficult to correctly identify the species in the field, which may lead to an underestimation of <i>R. nu</i> in Brazilian territory. This study aimed to address these questions by using a molecular approach to identify Plusiinae caterpillars throughout three seasons in non-<i>Bt</i>, Cry1Ac, and Cry1Ac × Cry1F soybean fields. Here, we carried out a comprehensive spatial sampling of the primary soybean-producing regions in Brazil. The results showed that <i>R. nu</i> has been the main Plusiinae occurring in soybean over the last three years, and it was present in all sampled regions. For Cry1Ac and Cry1Ac × Cry1F soybeans, up to 99% of the samples collected in 2023/24 were identified as <i>R. nu</i>. Non-<i>Bt</i> soybeans had higher variations in the proportion of the two species among the regions and across seasons, indicating that populations of <i>C. includens</i> and <i>R. nu</i> are co-occurring throughout the country. This is, to our knowledge, the most robust report assessing the distribution of <i>C. includens</i> and <i>R. nu</i> in Brazil using a molecular tool. This study provides clarification of <i>R. nu</i> occurrence and highlights the importance of pest monitoring from an integrated pest management perspective.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12027875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptability of Yuanjiang River Valley <i>Danaus genutia</i> to Different Host Plants in Yunan.","authors":"Jun Yao, Ting Du, Yangyang Li, Chengli Zhou, Lei Shi","doi":"10.3390/insects16040368","DOIUrl":"https://doi.org/10.3390/insects16040368","url":null,"abstract":"<p><p>This study aims to determine the local host plant range for Yuanjiang River Valley <i>Danaus genutia</i> (Cramer, 1779) and evaluate the effects of different host plants on its growth, development, and reproductive success. This research assesses adult oviposition preferences and larval feeding tendencies on various host plants. An age-stage, two-sex life table was constructed to analyze the butterfly's growth, development, reproductive success, survival rates, and other physiological aspects on different host plants. Population dynamics over the next 60 days were simulated to offer a comprehensive evaluation of the host plant suitability for <i>D. genutia</i>. The results indicated that <i>D. genutia</i> adults preferred laying eggs on <i>Cynanchum corymbosum</i>, followed by <i>C. annularium</i>, <i>C. rostellatum</i>, and <i>Asclepias curassavica</i>, with very few eggs laid on <i>Calotropis gigantea</i> and <i>Dregea volubilis</i>. Larval feeding preferences were observed in the order of <i>C. annularium</i> > <i>C. corymbosum</i> > <i>C. rostellatum</i>, with <i>A. curassavica</i> consumed only by first-instar larvae, while <i>C. gigantea</i> and <i>D. volubilis</i> were avoided. Under identical room temperature conditions, larvae feeding on <i>C. annularium</i> and <i>C. corymbosum</i> completed a full generation. Larvae feeding on <i>C. annularium</i> exhibited shorter developmental periods and higher early survival rates compared to those feeding on <i>C. corymbosum</i>, with no significant differences in adult longevity, sex ratio, or number of eggs per female. Female adults did not die immediately after laying eggs. No significant differences in the intrinsic rate of increase (<i>r</i>), finite rate of increase (<i>λ</i>), net reproduction rate (<i>R</i>₀), or doubling time (<i>T_d</i>) were found between populations feeding on the two host plants. However, the gross reproductive rate (<i>GRR</i>) and mean generation time (<i>T</i>) were significantly lower in the population feeding on <i>C. corymbosum</i>. These results confirm <i>C. annularium</i> and <i>C. corymbosum</i> as host plants for <i>D. genutia</i> larvae and provide a theoretical and scientific basis for the conservation of the Yuanjiang River Valley <i>D. genutia</i> population.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12027793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microhabitat Selectivity of Mites (Acari) in a Natural Lowland Beech Forest (<i>Melico</i>-<i>Fagetum</i>) in Wronie Reserve (Poland).","authors":"Radomir Graczyk, Sławomir Kaczmarek, Tomasz Marquardt, Krzysztof Gęsiński, Dariusz J Gwiazdowicz","doi":"10.3390/insects16040364","DOIUrl":"https://doi.org/10.3390/insects16040364","url":null,"abstract":"<p><p>The European beech (<i>Fagus sylvatica</i> L.) is a tree species common throughout Europe, with the eastern boundary of its range extending across Poland. Samples were collected in several microhabitats of beech stands (e.g., leaf litter, rotting wood, moss growing on tree stumps) in the Wronie Forest Reserve. The aim of this study was to determine the effect of microhabitat conditions found in beech forests on the diversity of mite assemblages and their species richness. The collected samples comprised a total of 144 identified species (78 species of Oribatida, 66 species of Mesostigmata), represented by 74,433 mite individuals (71,124 Oribatida and 3309 Mesostigmata). All the analyzed microhabitats varied in terms of their mite assemblages. The highest number of species was identified in moss on beech stumps (72 species-53 Oribatida and 19 Mesostigmata) and in samples collected from beech litter (68 species-48 Oribatida and 20 Mesostigmata). The most numerously represented species in the analyzed material was <i>Parachipteria willmanni</i>, which was classified to superdominants in moss on beech stumps and moss on beech trunks (0.5 and 2.0 m), which were the most similar microhabitats. In contrast, mite assemblages in rotting wood and marsh litter differed greatly.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12028256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Honey Bees Can Use Sequence Learning to Predict Rewards from a Prior Unrewarded Visual Stimulus.","authors":"Bahram Kheradmand, Ian Richardson-Ramos, Sarah Chan, Claudia Nelson, James C Nieh","doi":"10.3390/insects16040358","DOIUrl":"https://doi.org/10.3390/insects16040358","url":null,"abstract":"<p><p>Learning to anticipate upcoming events can increase fitness by allowing animals to choose the best course of action, and many species can learn sequences of events and anticipate rewards. To date, most studies have focused on sequences over short time scales such as a few seconds. Whereas events separated by a few seconds are easily learned, events separated by longer delays are typically more difficult to learn. Here, we show that honey bees (<i>Apis mellifera</i>) can learn a sequence of two visually distinct food sources alternating in profitability every few minutes. Bees were challenged to learn that the rewarded pattern was the one that was non-rewarded on the prior visit. We show that bees can predict and choose the feeder that will be rewarding upon their next approach more frequently than predicted by chance, and they improve with experience, with 64% correct choices made in the second half of their visit sequence (<i>N</i> = 320 visits by 20 different bees). These results increase our understanding of honey bee visual sequential learning and further demonstrate the flexibility of foragers' learning strategies.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12027691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunotranscriptomic Profiling of <i>Spodoptera frugiperda</i> Challenged by Different Pathogenic Microorganisms.","authors":"Yan Tang, Qi Zou, Guojie Yu, Feng Liu, Yu Wu, Xueyan Zhao, Wensheng Wang, Xinchang Liu, Fei Hu, Zengxia Wang","doi":"10.3390/insects16040360","DOIUrl":"https://doi.org/10.3390/insects16040360","url":null,"abstract":"<p><p><i>Spodoptera frugiperda</i> is a globally significant migratory agricultural pest that requires proactive monitoring. Understanding the molecular mechanisms underlying the interactions between pathogenic microorganisms and <i>S. frugiperda</i> is crucial for enhancing the effectiveness of microbial control agents against this pest. This study used transcriptome sequencing and molecular biology techniques on <i>S. frugiperda</i> larvae infected by bacteria and fungi to investigate the composition and molecular regulatory mechanisms of its immune system. A total of 598 immune-related genes were identified. Upon microbial infection, most immune-related genes showed an upregulated expression trend. Phylogenetic analysis revealed that the immune gene repertoire of <i>S. frugiperda</i> is relatively conserved. The expression of the genes of <i>peptidoglycan recognition proteins</i> in different tissues of <i>S. frugiperda</i> induced by microorganisms at different times was verified using qPCR, and the results confirmed that these genes were significantly upregulated under specific pathogenic infections. This study elucidates the immune transcriptome of <i>S. frugiperda</i> in response to various pathogenic microorganisms, providing valuable insights for improving the effectiveness of existing microbial agents and developing new, highly efficient, and specific biopesticides.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12028137/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MPF Regulates Oocyte and Embryo Development During Parthenogenesis Induction in Silkworm, <i>Bombyx mori</i>.","authors":"Chenkai Ma, Fang Xu, Chengjie Hu, Chunguang Cui, Xin Du, Jine Chen, Linbao Zhu, Shaofang Yu, Xingjian He, Wei Yu, Yongqiang Wang, Xia Xu","doi":"10.3390/insects16040361","DOIUrl":"https://doi.org/10.3390/insects16040361","url":null,"abstract":"<p><p>In most species, oocytes are arrested at the prophase or metaphase of meiosis I and require sperm-derived or external stimuli to resume meiosis. Maturation-promoting factor (MPF) is an oocyte maturation factor composing the catalytic subunit Cdc2 and the regulatory subunit CycB that can restart stalled meiosis. In this study, we demonstrated that MPF activity affected parthenogenesis induction in the model lepidopteran insect <i>Bombyx mori</i> using activator and inhibitor interference. We found that the upregulation of MPF activity significantly increased the parthenogenesis induction rate, whereas downregulation significantly reduced it. Furthermore, the inhibition of MPF activity also led to a delay in embryonic development. Given its evolutionary conservation, MPF emerges as a potential universal target for manipulating reproductive outcomes, offering broad applications in genetics and selective breeding.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12027660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contribution to the Diversity of the Genus <i>Sarcophaga</i> (Diptera: Sarcophagidae): Checklist, Species Distribution, and New Records for Greece.","authors":"Savvas Zafeiriou, Gabriella Dimitra Rakopoulou, Georgios Agapakis, Theodora Petanidou, Sotiris Alexiou","doi":"10.3390/insects16040359","DOIUrl":"https://doi.org/10.3390/insects16040359","url":null,"abstract":"<p><p>The current study presents new records and an updated checklist of the genus <i>Sarcophaga</i> (Diptera: Sarcophagidae) for Greece. The records were compiled from published literature, identification of newly collected material obtained through passive (trapping) and active (hand collecting, net sweeping) sampling conducted between 2018 and 2024, and the examination of material from two museum collections: Entomological Collection of the Goulandris Natural History Museum (1983-1987) and the Melissotheque of the Aegean (2004-2024). After the examination of 927 male specimens from 108 localities and the synthesis of the available literature, a total of 72 species distributed across 18 subgenera were documented. Among these, <i>Sarcophaga ferox</i> (Villeneuve, 1908) and <i>Sarcophaga anaces</i> (Walker, 1849) are newly records for Greece, while <i>Sarcophaga beckiana</i> (Lehrer, 1996) represents the first documented occurrence of the species in Europe. The findings represent the most contemporary contribution to the understanding of the species diversity and distribution of the genus <i>Sarcophaga</i> in Greece.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12027789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fly in the Ointment: Host-Specificity Challenges for <i>Botanophila turcica,</i> a Candidate Agent for the Biological Control of Saffron Thistle in Australia.","authors":"Vincent Lesieur, Thierry Thomann, Mireille Jourdan, Javid Kashefi, Marie-Claude Bon","doi":"10.3390/insects16040357","DOIUrl":"https://doi.org/10.3390/insects16040357","url":null,"abstract":"<p><p>In classical biological control of weeds, the risk posed by a candidate agent to close relatives of the target weed in the intended area of release is a key criterion (i.e., candidate agents that demonstrate a high degree of host specificity). In this study, we investigated if the rosette crown-feeding fly <i>Botanophila turcica</i> Hennig (Diptera: Anthomyiidae) could meet this criterion and thus be considered a good candidate to control saffron thistle <i>Carthamus lanatus</i> L. (Asteraceae: Cardueae) in Australia. Previous studies indicated that <i>B. turcica</i> is specific to <i>Ca. lanatus</i> and did not infest the closely related crop, safflower (<i>Carthamus tinctorius</i> L.). However, more recent field observations made in Greece reported that <i>B. turcica</i> infested safflower in cultivated fields. To determine if <i>B. turcica</i> is safe for release as a biocontrol agent, we re-examined the host range of <i>B. turcica</i> by performing new host-specificity testing combined with field surveys carried out in the south of France during two consecutive years. We also investigated the species identity of the flies by comparing DNA sequences (<i>COI</i> barcode region) of specimens collected in France from <i>Ca. lanatus</i> and <i>Centaurea solstitialis</i> L. with those from Greece collected from <i>Ce. solstitialis</i> and <i>Centaurea diffusa</i> Lam. Our <i>COI</i> analyses confirmed that French and Greek samples identified as <i>B. turcica</i> belonged to the same species, while a second group of Greek samples matched <i>B. brunneilinea</i>, indicating two distinct species. Our results also demonstrated that <i>B. turcica</i> has a wider host range than previously suggested. Laboratory testing indicated that <i>Ca. lanatus</i>, <i>Ca. tinctorius,</i> and <i>Ce. solstitialis</i> are suitable for the development of <i>B. turcica</i>. Field surveys also revealed that <i>Ce. diffusa</i> is part of the host range of the fly. Based on the results reported here, <i>B. turcica</i> may have the potential to control both the target weed, <i>Ca. lanatus,</i> and <i>Ce. Solstitialis,</i> but it may also be a threat to safflower, <i>Ca. tinctorius</i>. Further investigations to assess under what conditions <i>B. turcica</i> attacks <i>Ca. tinctorius</i> may help clarify the level of risk to Australian growers.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12028163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}