Insects最新文献

{"title":"Expression Characteristics of Gustatory Receptor Genes in <i>Galeruca daurica</i> (Coleoptera: Chrysomelidae) and Adult Behavioral and Electrophysiological Responses to Host Metabolites.","authors":"Jing Gao, Jinwei Li, Haichao Wang, Jinghang Zhang, Xiaomin An, Yanyan Li, Jun Zhao, Baoping Pang, Ling Li","doi":"10.3390/insects17040442","DOIUrl":"https://doi.org/10.3390/insects17040442","url":null,"abstract":"<p><p><i>Galeruca daurica</i> (Joannis) (Coleoptera: Chrysomelidae) is an oligophagous pest in which both adults and larvae prefer to feed on <i>Allium</i> forage grasses of the Liliaceae family. In this study, we identified gustatory receptor (GR) genes based on the transcriptome data of <i>G. daurica</i>; analyzed the expression profiles of these GR genes across different larval instars and various tissues of male and female adults using quantitative real-time PCR (qRT-PCR); detected the electrophysiological responses of the mouthparts of male and female <i>G. daurica</i> adults to flavonoids and carbohydrates using single sensillum recording (SSR); and recorded the changes in food consumption of <i>G. daurica</i> adults after feeding on six host plant-derived metabolites. A total of 26 GR genes were identified from the transcriptome data of adult and larval of <i>G. daurica</i>. Phylogenetic analysis was performed to screen candidate functional gustatory receptor genes, including four sugar receptors (<i>GdauGR7</i>, <i>GdauGR10</i>, <i>GdauGR14</i> and <i>GdauGR28</i>), seven bitter receptors (<i>GdauGR11</i>, <i>GdauGR16~17</i>, <i>GdauGR22</i>, <i>GdauGR25~26</i> and <i>GdauGR30</i>), and two CO₂ receptors (<i>GdauGR15</i> and <i>GdauGR20</i>). Larval expression profiling of <i>GdauGRs</i> in <i>G. daurica</i> revealed that the relative expression levels of 17 genes exhibited dynamic changes during larval growth and development. <i>GdauGRs</i> were expressed to varying degrees in the antennae, mouthparts, brain, gut, and forelegs of adult <i>G. daurica</i>, with sex-specific differences. Notably, the expression levels of <i>GdauGR4</i>, <i>GdauGR9</i> and <i>GdauGR16</i> in the gut were extremely significantly higher than those in other tissues. In the SSR test, the six tested flavonoids and one carbohydrate were able to induce robust electrophysiological responses in the gustatory sensilla on the antennae and mouthparts of adult <i>G. daurica</i> at specific concentrations. In addition, the supplementation of several host-derived metabolites altered the food consumption of adult <i>G. daurica</i>. These findings lay a solid foundation for elucidating the molecular mechanisms underlying gustatory recognition and host adaptation in <i>G. daurica</i>.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"17 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13116256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Pest Management Strategies for Controlling <i>Phthorimaea</i> (<i>Tuta</i>) <i>absoluta</i>: Advances in Biological, Pheromone, and Cultural Control Methods.","authors":"Chen Zhang, Yu-Xin Wang, Xu-Dong Liu, Asim Iqbal, Qing Wang, Yu Wang","doi":"10.3390/insects17040441","DOIUrl":"https://doi.org/10.3390/insects17040441","url":null,"abstract":"<p><p>The tomato leaf miner, <i>Phthorimaea</i> (<i>Tuta</i>) <i>absoluta</i>, Meyrick 1917 is recognized as a highly destructive pest, causing significant economic losses to crops in both greenhouse and open field environments across four continents: Asia, Africa, Europe, and South America. High genetic homogeneity among populations from various regions and countries indicates significant gene flow between <i>P. absoluta</i> populations, suggesting a lack of geographical barriers to dispersion. Furthermore, <i>P. absoluta</i> has developed resistance to insecticides due to target-site mutations or metabolic resistance, which enable the insect to withstand lethal doses of insecticides. To control this insect pest, the plant-mediated RNA interference (RNAi) is most promising host-induced gene silencing technique, utilized the plant's machinery to express double-stranded (dsRNA), which triggers the RNAi pathway in <i>P. absoluta</i>. Due to thermal tolerance, the <i>P. absoluta</i> has increased its area of invasion by 600 km per year over 9 years. Female <i>P. absoluta</i> releases pheromones that are recognized by males with a sophisticated olfactory circuit on their antenna. Pheromone binding proteins (PBPs) play a crucial role in mate recognition and attraction, and their expression peaks during courtship, specifically around 6:00 a.m. Given its potential to significantly alter the insect genome, clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) offer a revolutionary strategy to control <i>P. absoluta</i>. Furthermore, this pest has developed remarkable adaptations to survive on unfavorable hosts by secreting specific proteins from its salivary glands that detoxify plant defenses. Insecticide resistance is likely the cause of field control failures of <i>P. absoluta</i>. Biological control, sex pheromone traps, and cultural control are the most promising approaches to address insecticide resistance resulting from these failures. Therefore, the implementation of integrated control programs and appropriate resistance management strategies is necessary to keep <i>P. absoluta</i> infestations under economic damage thresholds.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"17 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13116861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the Effects of Thymol and Oxalic Acid on Honey Bee Colony Condition Using Ratiometric Spectral Indicators in Honey and Beeswax.","authors":"Mira Stanković, Miroslav Nikčević, Sladjana Z Spasić, Ksenija Radotić","doi":"10.3390/insects17040440","DOIUrl":"https://doi.org/10.3390/insects17040440","url":null,"abstract":"<p><p>Over the past 20 years, honey bee colony declines have been driven by multiple factors, notably diseases and parasites. The parasitic mite <i>Varroa destructor</i>, which weakens the bees' immune systems, has been particularly harmful. While various synthetic acaricides are used, the chemicals may accumulate in the beeswax, endangering colony health and allowing <i>Varroa</i> populations to develop resistance to these acaricides. These problems have prompted interest in organic alternatives like thymol and oxalic acid. In this study, colony health was assessed through the proteins-to-phenolics spectral ratio in honey and beeswax, determined by fluorescence spectroscopy, as a ratiometric indicator of infection level in treated hives. Over two months, hives were treated with either oxalic acid, thymol, or remained untreated as controls. Neither treatment significantly affected the proteins-to-phenolics ratios in honey, ranging from 0.30 to 0.83, or in beeswax, ranging from 1.40 to 1.83, suggesting that the incorporation of these vital constituents remains stable despite acaricide application. While thymol demonstrates potential adverse effects on bee health, careful management of treatment concentrations is essential to ensure both the efficacy of <i>Varroa</i> control and the preservation of honey quality. These findings provide valuable insights for beekeepers regarding the safe application of organic acaricides.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"17 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13117002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insect SVWC Proteins: A Diverse Cytokine-like Family Orchestrating Multilayered Antiviral and Antibacterial Immunity.","authors":"Yangyang Chen, Gaoying Xu, Jingao Wang, Cong Zhang, Aliyu Yusuf Abubakar, Hengchuan Xia","doi":"10.3390/insects17040438","DOIUrl":"https://doi.org/10.3390/insects17040438","url":null,"abstract":"<p><p>The single von Willebrand factor C (SVWC) domain-containing protein family represents a crucial class of immune molecules recently identified in insects and crustaceans. Initially regarded as functional analogs of vertebrate interferons (IFNs) due to their virus-induced expression and activation of the Janus kinase-signal transducer and activator of the transcription (JAK-STAT) pathway, recent studies have revealed that SVWC proteins possess far more complex functions. Many SVWC members are themselves a novel class of pattern recognition receptors (PRRs) that can directly bind to viruses and bacteria. Importantly, SVWCs are not a single entity but a highly diverse family-multiple subtypes exist in <i>Drosophila</i>, <i>Bombyx mori</i>, and shrimp-a gene expansion that implies functional differentiation. This review systematically examines the multifunctionality of SVWC proteins in insects and crustaceans, with a particular focus on the functional specialization driven by subtype diversity. We delve into the complex regulatory networks governing SVWC expression, including the differential activation by nuclear factor kappa B (NF-κB) pathways (Dorsal, Rel-2, Relish) and interferon regulatory factor (IRF) pathways. We detail the unique signaling mechanism by which SVWCs activate the JAK-STAT pathway via integrins, rather than the canonical Domeless receptor. Furthermore, we extend the discussion to the emerging roles of SVWCs as PRRs in humoral immunity (activating Toll/IMD pathways to induce antimicrobial peptides) and cellular immunity (mediating hemocyte phagocytosis). Based on current evidence, We propose that diverse SVWC subtypes may recognize distinct pathogens, bind to different integrin receptors, and activate specific STAT variants via disparate upstream induction pathways, thereby establishing a systematic and hierarchical immunoregulatory network. This understanding positions the SVWC protein family as a central hub in the insect immune network and offers a novel perspective on the complexity and evolution of invertebrate immunity.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"17 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13115929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNAi-Mediated Silencing of vATPase Subunit E Impairs Larval Development in <i>Plutella xylostella</i>, and Virtual Screening Identifies a Potential Inhibitor.","authors":"Xuetao Yu, Jinhua Luo, Jiayi Xue, Lin Lu, Pan Deng, Li Zhu, Kang Yang, Xia Wan, Yuhua Wu, Akmal Boboev, Gang Wu, Xiaohong Yan, Chenhui Shen","doi":"10.3390/insects17040439","DOIUrl":"https://doi.org/10.3390/insects17040439","url":null,"abstract":"<p><p>The diamondback moth (<i>Plutella xylostella</i>) is a devastating global pest of cruciferous crops. This study explores the potential of targeting the vacuolar ATPase subunit E (<i>PxvATPaseE</i>) for its control. We demonstrate that <i>PxvATPaseE</i> is essential for larval development, showing high expression levels in the midgut. RNA interference (RNAi)-mediated silencing of <i>PxvATPaseE</i> resulted in severe growth retardation and dose-dependent mortality, with higher dsRNA doses inducing more sustained effects. Furthermore, computational virtual screening of natural compound libraries identified a high-affinity binder of <i>Px</i>vATPaseE, such as periplocoside D. Our results demonstrate that <i>PxvATPaseE</i> is a promising molecular target for controlling <i>P. xylostella</i>, supporting a dual-strategy approach combining RNAi and targeted chemical inhibition for future pest management solutions.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"17 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13116757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contrasting Toxicity Classes Differentially Affect Gut Microbiota Composition in Honey Bees.","authors":"Yunchao Kan, Ruoke Wang, Bing Zhang, Yu Liu, Runqiang Liu, Zhongyin Zhang, Zhaonan Zhang, Camilo Ayra-Pardo, Dandan Li","doi":"10.3390/insects17040437","DOIUrl":"https://doi.org/10.3390/insects17040437","url":null,"abstract":"<p><p>Honey bees rely on a specialized gut microbiota for nutrition, detoxification, and immune function, yet the effects of emerging insecticides on this symbiotic system remain poorly understood. We compared the acute toxicity and short-term gut microbiota responses of <i>Apis mellifera ligustica</i> workers exposed to two insecticides with contrasting toxicity classes: the highly toxic emamectin benzoate-lufenuron (EB-LFR) and the low-toxicity ecdysone agonist RH-5849. EB-LFR was associated with observed reductions in core gut symbionts (<i>Gilliamella</i>, <i>Snodgrassella</i>, <i>Lactobacillus</i>), a transient increase in <i>Bifidobacterium</i>, and the detection of opportunistic taxa such as <i>Serratia marcescens</i> and <i>Enterobacter hormaechei</i>. In contrast, RH-5849 was associated with broad reductions in beneficial bacteria without detectable pathogen emergence, suggesting a more moderate alteration of microbiota composition. Because microbiota analyses were based on single pooled samples per treatment, these results represent exploratory, qualitative insights into early microbial responses. Together with acute toxicity data, the findings suggest that insecticides with contrasting toxicity classes may differentially affect gut microbiota composition in honey bees and highlight the value of incorporating gut microbiota endpoints into pesticide risk-assessment frameworks to better anticipate sublethal effects on pollinator health.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"17 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13116194/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable Rearing of <i>Tenebrio molitor</i> Larvae Using Peatland Biomass.","authors":"Asma Akaichi, Nazanin Fazel Dehkordi, Jan Berend Lingens, Alexandra Rath, Florian Lohkamp, Amr Abd El-Wahab, Marwa F E Ahmed, Nils Th Grabowski, Kashif Ur Rehman, Madeleine Plötz, Christian Visscher, Cornelia Schwennen","doi":"10.3390/insects17040436","DOIUrl":"https://doi.org/10.3390/insects17040436","url":null,"abstract":"<p><p>To promote sustainable biomass recycling and support food security, <i>Tenebrio molitor</i> (TM) larvae can serve as an eco-friendly source of food and feed. This study compared the survival, growth performance, and nutritional composition of TM larvae fed five diets. The control (CON) diet contained distillers' dried grains with solubles (DDGS) and wheat bran (WB), while the experimental diets included 10-40% lignocellulose-rich organic products from rewetted peatlands (LPRP) replacing WB, with DDGS adjusted to maintain equivalent protein levels (about 21%). A total of 2500 larvae were divided into five replicates per treatment (100 larvae each). Survival exceeded 90% across all groups. Larvae fed the CON diet had a higher final body weight than those on the 30% and 40% LPRP diets (<i>p</i> < 0.05), with no significant differences among the CON and 10% and 20% LPRP groups. The feed conversion ratio (fresh matter) was significantly lower in the CON and 10% LPRP groups than in the other groups (<i>p</i> < 0.05). Larvae fed the 10% LPRP diet showed slightly higher crude protein content (55.8%) compared to the control group (54.8%) and the other treatment groups, whereas those fed the 30% LPRP diet had the highest numerical total amino acid content. Taken together, these results indicate that incorporating 10% LPRP with DDGS and WB provides the best overall balance between growth performance and nutritional quality for TM larvae, supporting sustainable production and circular economy goals.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"17 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13116621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A True Bug with a True but Unique Chela in 100 Million-Year-Old Amber.","authors":"Carolin Haug, Fenja I Haug, Marie K Hörnig, Florian Braig, Joachim T Haug","doi":"10.3390/insects17040431","DOIUrl":"https://doi.org/10.3390/insects17040431","url":null,"abstract":"<p><p>Grasping appendages in representatives of the group Insecta are usually of the sub-chelate (jackknifing) type. Only rarely do chelate appendages occur: <i>Carcinothrips</i> (Thysanoptera), Dryinidae (Hymenoptera), and Carcinocorini (Heteroptera). Here, we report a new find from about 100 million-year-old Kachin amber, representing, to our knowledge, the first case of a fossil of the group Insecta with a chelate type of appendage. This find is only the fourth case of an independent or convergent evolution of such a structure. A quantitative morphological comparison of over 2000 grasping structures reveals that the proximal part of the chela (in this case, the femur) has a unique shape in the fossil, unparalleled in the fossil and modern fauna. Moreover, some other new fossils reported do not possess such a shape. The mouthparts, forming a distinct beak, allow us to identify the new fossil with the distinct chela as a representative of Heteroptera. Its short beak, together with some other characteristics, indicates that it most likely is a representative of Nepomorpha. Many other details of the fossil are not well preserved, but the overall appearance shows similarities to extant and fossil representatives of Gelastocoridae, a group of terrestrial predators.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"17 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13116665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Edible Insects as Human Food: Perceptions of Individuals from Six Countries.","authors":"Raquel P F Guiné, Sofia G Florença, Anayansi Escalante-Aburto, Rosa María Mariscal-Moreno, César Ozuna, Lucio Rodríguez-Sifuentes, Cristina Chuck-Hernández, Marijana Matek Sarić, Nada M Boustani, Elena Bartkiene, Cristina Filip, Simona Pârvu, Monica Tarcea","doi":"10.3390/insects17040434","DOIUrl":"https://doi.org/10.3390/insects17040434","url":null,"abstract":"<p><p>Edible insects (EIs) have been suggested as a sustainable alternative to meat from traditional sources. However, despite EIs being consumed by humans since time immemorial, it is also true that, in many areas, especially in Western countries, there is some resistance and even neophobia to their acceptance as food. This work aimed to investigate the perceptions about EIs and validate a corresponding scale. This descriptive cross-sectional study was carried out by means of a questionnaire survey on a sample of 3711 participants in six countries (Croatia, Lebanon, Lithuania, Mexico, Portugal and Romania). Data were analysed by factor and cluster analyses, as well as other statistical tools. The results showed that, out of the initial 36 items in the questionnaire, 24 were validated in the scale of perceptions, distributed across six factors. Cluster analysis showed that the participants could be grouped according to three clusters (Traditionalists, Shoppers and Innovators). Statistical tests revealed significant differences (<i>p</i> < 0.05) between the clusters in practically all sociodemographic and consumption variables studied. Finally, a word analysis of the 2263 words indicated by the participants when thinking about EIs revealed that the most frequent words were 'disgusting', 'protein', and 'nutritive' in all three clusters. However, differences were observed after the fourth position in the list of most frequent words. In conclusion, these results indicate that perceptions of EIs were highly variable across countries of origin and other sociodemographic groups, and that, globally, participants exhibited some neophobia towards EIs. However, they recognised their value in terms of nutrition, particularly protein. This work evidences some relevant aspects that can be useful to adapt and define targeted policies to demystify the negative perceptions about EIs and improve consumer acceptance, thus contributing to more sustainable food systems.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"17 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13116201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Faunistic Contributions to the Superfamilies Oestroidea and Muscoidea (Insecta: Diptera) of Greece and Cyprus: New Records from Five Calyptrate Families.","authors":"Gabriella Dimitra Rakopoulou, Savvas Zafeiriou, Nikoleta-Nefeli Kofou, Theodora Petanidou, Georgios Agapakis","doi":"10.3390/insects17040433","DOIUrl":"https://doi.org/10.3390/insects17040433","url":null,"abstract":"<p><p>Knowledge of the Oestroidea and Muscoidea fauna of Greece and Cyprus remains fragmentary, with substantial parts of the two countries having never been systematically surveyed. The present study verifies the presence of <i>Scathophaga stercoraria</i> (Linnaeus, 1758) in Cyprus and records 16 new species from Greece, belonging to five calyptrate families: [<i>Anthomyia illocata</i> Walker, 1856 (Muscoidea: Anthomyiidae); <i>Scathophaga lutaria</i> (Fabricius, 1794) (Muscoidea: Anthomyiidae); <i>Fannia pallitibia</i> (Rondani, 1866); <i>Fannia pusio</i> (Wiedemann, 1830) (Muscoidea: Fanniidae); and <i>Coenosia</i> sp. nov. 1, <i>Coenosia</i> sp. nov. 2, <i>Lispe flavicincta</i> Loew, 1847, <i>Lispe nuba</i> Wiedemann, 1830, <i>Lispe orientalis</i> Wiedemann, 1824, <i>Lispe</i> cf. <i>sericipalpis</i> (Stein, 1904), <i>Potamia littoralis</i> Robineau-Desvoidy, 1830 (Muscoidea: Muscidae); <i>Apodacra radchenkoi</i> Verves and Khrokalo, 2015, <i>Craticulina tabaniformis</i> (Fabricius, 1805), <i>Miltogramma rutilans</i> Meigen, 1824, <i>Nyctia lugubris</i> (Macquart, 1843) (Oestroidea: Sarcophagidae), and <i>Linnaemya lithosiophaga</i> (Rondani, 1859) (Oestroidea: Tachinidae)]. These records are based on the examination of 152 dry-pinned specimens from 58 localities, collected between 1978 and 2026 across Greece and Cyprus using a combination of passive (animal-baited traps, UV-bright pan traps) and active (hand collecting, net sweeping) sampling methods, together with insect material from the entomological collections of the National Museum of Natural History Goulandris and the Melissotheque of the Aegean. In addition, the first checklists of the family Fanniidae and the subfamily Scathophaginae for Greece and Cyprus are presented. Collectively, the findings presented expand the documented diversity of Greek and Cypriot Calyptratae and refine the current understanding of their biogeographic patterns, providing an updated framework for taxonomic, ecological, forensic, and other applied entomological research within the two countries.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"17 4","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13115656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}