Insects最新文献

{"title":"Occurrence and Genetic Variation of <i>Monolepta hieroglyphica</i> (Motschulsky, 1858) (Coleoptera: Chrysomelidae), a Newly Emerging Pest, Among Hosts in Northeast China.","authors":"Wei Sun, Xiuhua Zhang, Jiachun Zhou, Yuebo Gao","doi":"10.3390/insects16060605","DOIUrl":"10.3390/insects16060605","url":null,"abstract":"<p><p>The northeast region of China plays a crucial role in crop production. The leaf beetle <i>Monolepta hieroglyphica</i> (Motschulsky, 1858) (Coleoptera: Chrysomelidae) has emerged as a potential threat to food security in the region. With a wide distribution spanning Asia and Russia, this beetle affects various crops. However, limited information is available regarding its occurrence patterns and genetic diversity among major crops in the region. Based on systematic observations across various hosts, coupled with genetic variation analysis using mitochondrial DNA markers, the main results were as follows. Leaf beetle occurrence varied among hosts, peaking from late July to mid-August, with maize and soybean fields exhibiting higher infestation rates compared with other crops. Notably, late-cultivated maize fields harbored the highest beetle numbers due to the species' preference for young leaves. The host transfer trajectory may have originated in soybean and weeds, with subsequent alternation between host plants and other crops, before the final migration to cabbage and late-cultivated maize fields. Genetic analysis revealed nine COI haplotypes, four COII haplotypes, eleven Cytb haplotypes, and twenty-one combined haplotypes. No clear relationship existed between genetic diversity and occurrence, and no distinct host-based genetic patterns emerged from neighbor-joining tree and haplotype network analyses. High gene flow rates were observed, likely contributing to decreased genetic variation. An analysis of molecular variance results indicated major genetic variation within populations, although genetic distance and haplotype distribution indicated divergence among host populations. These results provide foundational data for developing effective <i>M. hieroglyphica</i> pest management strategies.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytogenetic and Molecular Characterization of <i>Sphaerophoria rueppellii</i> (Diptera, Syrphidae).","authors":"Pedro Lorite, José M Rico-Porras, Teresa Palomeque, Mª Ángeles Marcos-García, Diogo C Cabral-de-Mello, Pablo Mora","doi":"10.3390/insects16060604","DOIUrl":"10.3390/insects16060604","url":null,"abstract":"<p><p><i>Sphaerophoria rueppellii</i> is a Palearctic hoverfly widely used as a native biocontrol agent against aphid pests in Mediterranean agroecosystems. In this study, we present a cytogenetic analysis and characterization of the mitochondrial genome of this species. Chromosomal preparations, obtained from third-instar larvae, were used for conventional staining, DAPI staining and C-banding techniques, and major ribosomal DNA (rDNA) location by fluorescence in situ hybridization (FISH). Karyotype analysis revealed a diploid number of 2n = 10, with heterochromatic blocks in the pericentromeric regions of all autosomes and rDNA clusters on both sex chromosomes. The complete mitochondrial genome (16,605 bp) was sequenced and annotated using next-generation sequencing and assembly pipelines. It contains the typical 37 mitochondrial genes and a highly A + T-rich control region with tandem repeats. Gene order and codon usage were conserved compared with other Syrphidae. Phylogenetic reconstruction based on mitochondrial protein-coding genes clarifies the species' placement within the Syrphini tribe. Our results contribute valuable genomic and cytogenetic information that supports comparative analyses and may aid in taxonomic clarification within the genus. These findings also offer key data that could guide the genetic optimization of <i>S. rueppellii</i> as an efficient, environmentally safe biological control agent in sustainable agriculture.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diverse Sublethal Effects of a Common Fungicide Impact the Behavior and Physiology of Honey Bees.","authors":"Xufeng Zhang, Qian Cao, Feng Wang, Yinyin Du, Wen Zhao, Yuan Guo, Olav Rueppell","doi":"10.3390/insects16060603","DOIUrl":"10.3390/insects16060603","url":null,"abstract":"<p><p>Honey bees and other pollinators are key to functioning natural and managed ecosystems. However, their health is threatened by many factors, including pesticides. Spraying fungicides during flowering of fruit trees is widespread even though it directly exposes pollinators to these fungicides. Here, we report a series of experiments designed to understand how the combination of propiconazole and carbendazim, marketed in China as Chunmanchun^®, affects honey bee health. With an acute oral toxicity of 23.8 μg a.i./bee over 24 h in the laboratory, we considered the acute mortality risk from normal Chunmanchun^® applications as relatively low. However, our comprehensive studies revealed other diverse effects: Chunmanchun^® reduced memory after classic conditioning by approximately 25% and altered the activity of protective enzymes and the composition of the honey bees' gut microbiota. Specifically, the genus <i>Lactobacillus</i> was decreased by ~13%, and <i>Bartonella</i> and <i>Snodgrassella</i> were increased by ~10% and ~7.5%, respectively. The gut metabolome was also disrupted in diverse ways, possibly as a functional consequence of the microbiome changes. Thus, we demonstrated numerous sublethal effects of the combination of propiconazole and carbendazim, which adds to the growing evidence that agrochemicals and fungicides in particular can harm pollinator health in subtle ways that are not captured in simple mortality assays.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the Conservation Priority of Alpine Carabid Beetle Communities by Mapping the Index of Natural Value (INV) in Natura 2000 Habitats in the Brenta Dolomites (Italian Alps).","authors":"Emiliano Peretti, Marco Armanini, Roberta Chirichella, Andrea Mustoni, Mauro Gobbi","doi":"10.3390/insects16060602","DOIUrl":"10.3390/insects16060602","url":null,"abstract":"<p><p>The Dolomites (European Alps) are a UNESCO World Heritage Site known to harbor distinctive communities of carabid beetles adapted to high-altitude environments, whose composition is shaped mainly by landform and habitat type. We aimed to assess the conservation priority of carabid beetle communities in the Brenta mountain group (Italy). We used the Index of Natural Value (INV), based on the relative frequencies and abundances of highly specialized Alpine species, as a proxy of the sensitivity to disturbance, and, thus, to the vulnerability of the carabid beetle communities to extinction. We used information on Natura 2000 habitat cover to produce a map for identifying areas of the Brenta Dolomites inhabited by the carabid beetle communities most relevant for conservation. We also report on the main differences in terms of species composition between the investigated communities. We found a positive correlation between vulnerability and altitude. Specifically, the most vulnerable communities were recorded in high-altitude habitat types (limestone cliffs and pavements, calcareous and calcschist screes, and alpine calcareous grasslands). Alkaline fens resulted in having the least vulnerable community, while those in all other habitats (bush and forests) were found to have intermediate levels of vulnerability.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Analysis of the <i>UGT</i> Gene Superfamily in <i>Spodoptera frugiperda</i>.","authors":"Yang Liu, Minghui Guan, Kunliang Zou, Tonghan Wang, Haiyang Wang, Lu Sun, Bo Feng, Jiali Ding, Xiang Gao, Yongfu Wang, Degong Wu, Junli Du","doi":"10.3390/insects16060601","DOIUrl":"10.3390/insects16060601","url":null,"abstract":"<p><p>UDP-glycosyltransferases (UGTs) are widely distributed enzymes in living organisms that catalyze the transfer of glycosyl groups from donor molecules to acceptor molecules' glycoside ligands. These enzymes are pivotal for detoxifying and eliminating both endogenous and exogenous toxic substances in insects. In this study, bioinformatics methods were used to analyze the <i>UGT</i> gene superfamily in the fall armyworm (<i>Spodoptera frugiperda</i>), resulting in the identification of 48 <i>UGT</i> genes located across 10 chromosomes, including 23 tandem duplication pairs. The predicted SfUGT proteins mainly exhibit α-helical secondary structures. Intron numbers varied significantly, with high diversity observed in amino acid sequences. Phylogenetic analysis grouped <i>UGT</i> genes from three insect species into three distinct subfamilies, revealing a closer evolutionary relationship between <i>S. frugiperda</i> and <i>Spodoptera litura</i>, supported by a greater number of orthologous genes. Expression profiling showed that <i>SfUGT16</i> and <i>SfUGT21</i> are highly expressed in the first and fourth larval instars, respectively; <i>SfUGT16</i> is predominantly expressed in the Malpighian tubules and midgut, implying roles in digestion, metabolism, and detoxification. Meanwhile, <i>SfUGT21</i>, <i>SfUGT30</i>, and <i>SfUGT48</i> exhibited elevated expression in the hemolymph, suggesting functions in metabolism and transport, whereas <i>SfUGT40</i> showed high expression in both the midgut and hemolymph, indicating involvement in detoxification and metabolic processes. These findings provide a foundation for further exploration of the biological functions of the <i>UGT</i> gene family.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Report of a New Sand Fly (Diptera: Psychodidae) Species, <i>Sergentomyia</i> (<i>Neophlebotomus</i>) <i>pradeepii</i> n. sp. from Madhya Pradesh, India.","authors":"Harish Kumar Shah, Pananchikkaparambil Abdu Fathima, Manju Rahi, Prasanta Saini","doi":"10.3390/insects16060598","DOIUrl":"10.3390/insects16060598","url":null,"abstract":"<p><p>Madhya Pradesh, a biodiversity-rich state in central India, reports sporadic non-indigenous leishmaniasis cases. Systematic entomological surveillance as part of molecular xenomonitoring in sand flies led to the discovery of a new species, <i>Sergentomyia (Neophlebotomus</i>) <i>pradeepii</i> n. sp. (Diptera: Psychodidae), from Johariya village in Sagar district, Madhya Pradesh, India. A systematic cross-sectional survey of sand flies was conducted in Bhopal, Sagar, and Hoshangabad districts of Madhya Pradesh. Standard collection methods were employed for two months, i.e., from July to August 2023. DNA barcoding targeting the mitochondrial Cytochrome c oxidase subunit I (COI) gene was performed, and the generated sequences were phylogenetically analyzed. <i>Se.</i> (<i>Neo.</i>) <i>pradeepii</i>, a newly recorded sand fly species, is reported in this study. Its taxonomic relationship to other congeners of subgenus <i>Neophlebotomus</i> is discussed. COI barcoding and phylogenetic analysis established that the specimens fit into the same taxonomic group, exhibiting negligible gene flow within the population, while a 13.4% genetic distance from congeners establishes it as a separate species. Madhya Pradesh, with its rich biodiversity and favorable conditions for sand fly proliferation, lacks systematic entomological surveillance. This study enhances the knowledge of the state's sand fly fauna by reporting and providing a detailed morphological and molecular description of the new species.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Essential Oil from Cumin (<i>Cuminum cyminum</i>) Seeds and Selected Terpenes as Repellents Against Adult Female <i>Phlebotomus papatasi</i> (Scopoli) (Diptera: Psychodidae) Sand Flies.","authors":"Maia Tsikolia, Panagiota Tsafrakidou, Michael Miaoulis, Andrew Y Li, Dawn Gundersen-Rindal, Alexandra Chaskopoulou","doi":"10.3390/insects16060599","DOIUrl":"10.3390/insects16060599","url":null,"abstract":"<p><p>Leishmaniasis, a parasitic disease transmitted by sand flies, poses a significant global health threat. Chemical repellents and insecticides are widely used for protection, but prolonged use has led to resistance, reduced efficacy, and environmental concerns, emphasizing the need for new repellent compounds, ideally from sustainable sources. This study investigated the chemical composition and repellent properties of cumin seed essential oil (EO) from Greece against <i>Phlebotomus papatasi</i>. Gas chromatography-mass spectrometry (GC-MS) identified five major constituents, including cumin aldehyde (27.0%), <i>β</i>-pinene (11.4%), and <i>γ</i>-terpinene (10.8%). In addition to cumin seed EO and its major constituents, octanol, and 1-octen-3-ol were tested for comparison, along with transfluthrin and DEET as standard repellents. Using a static air repellency bioassay, cumin seed EO, cumin aldehyde, and octanol exhibited strong spatial repellency (EC₅₀ of 0.34, 0.07, and 0.60 μg/cm² respectively) comparable to transfluthrin (EC₅₀ of 0.04 μg/cm²) at 1 h, and contact repellency, both lasting up to 3 h. This is the first study to evaluate cumin seed EO and cumin aldehyde against sand flies, highlighting their potential as alternatives to conventional repellents. Further research is needed to explore their applicability in vector control strategies.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth Performance and Nutritional Content of Tropical House Cricket (<i>Gryllodes sigillatus</i> (Walker, 1969)) Reared on Diets Formulated from Weeds and Agro By-Products.","authors":"Henlay J O Magara, Sylvain Hugel, Brian L Fisher","doi":"10.3390/insects16060600","DOIUrl":"10.3390/insects16060600","url":null,"abstract":"<p><p>The tropical house cricket (<i>Gryllodes sigillatus</i>) can convert organic diets formulated from weeds and agro by-products into high-quality biomass. This study assessed the potential of diets developed from weeds and agro by-products as a feed source for <i>G. sigillatus</i>. We compared the development and nutritional value of crickets fed these alternative diets with control crickets fed chicken feed. Ten different diets with varying protein contents were used, including chicken feed (Control) with a protein content of 215 g/Kg dry matter (DM) basis), Cassava-Sugar Diet (250 g/Kg DM protein) Desmodium-Bran Diet (245 g/Kg DM protein), Morning Glory-Bean Diet (240 g/Kg DM protein), Morning Glory-Cassava Diet (235 g/Kg DM protein), Morning Glory-Cowpea Diet (225 g/Kg DM protein), Mixed Weed-Bran Diet (Optimal) (215 g/Kg DM protein) Cassava-Gallant Soldier Diet (200 g/Kg DM protein), Wheat-Bran Diet (145 g/Kg DM protein), and Maize-Cassava Diet (135 g/Kg DM protein). The weight and length of the crickets were measured for 9 weeks from day 1 after hatching to day 56. Then, the crickets were harvested and analyzed for dry matter, crude protein, fat, ash, fiber, minerals, and fatty acid composition. Cricket developmental time, survival rate, weight and length, yield, proximate components, and mineral and fatty acids differed depending on the diet provided. The Mixed Weed-Bran Diet (Optimal) resulted in the crickets developing faster (48.8 days), with a higher survival rate (88.1%), greater adult length (19.2 cm) and weight (0.44 g), and a nutrition content richer in minerals and unsaturated fatty acids when compared to other treatments. Oleic, linoleic, and palmitic acids were the major fatty acids. The highest protein content (64.4 g/100 g) was observed in the Mixed Weed-Bran Diet (Optimal) and Morning Glory-Cassava Diet treatments, while the Maize-Cassava Diet treatment crickets possessed the highest quantities of fats (19.1 g/100 g) and ash (15.4 g/100 g). The fatty acid profile of <i>G. sigillatus</i> revealed the cricket to have high unsaturated fatty acids except in crickets fed Morning Glory-Cowpea Diet and Wheat-Bran Diet. Generally, <i>G. sigillatus</i> grew best and had the most nutritious body composition on the Mixed Weed-Bran Diet (Optimal). The findings indicate that diets developed from weeds and agro by-products have great potential to be used as an alternative feed source for crickets and are capable of replacing expensive chicken feed, enhancing the circular farming potential of insect farming.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune Gene Expression and Locomotor Activity in Response to <i>Vairimorpha ceranae</i> Infection Across Five Honey Bee Subspecies.","authors":"Cansu Özge Tozkar, Jay D Evans","doi":"10.3390/insects16060593","DOIUrl":"10.3390/insects16060593","url":null,"abstract":"<p><p>This study evaluated immune gene expression and locomotor behavior across five <i>Apis mellifera</i> subspecies (Carniolan, Caucasian, Syrian, Muğla ecotype, and Yığılca ecotype) following controlled <i>Vairimorpha ceranae</i> infection. Six days post-infection, Caucasian, Carniolan, and Yığılca bees exhibited a significant upregulation of antimicrobial peptide (AMP) transcripts-hymenoptaecin, abaecin, defensin, and apidaecin-indicating a robust humoral response. Conversely, Syrian and Muğla bees showed weaker AMP expression and higher <i>V. ceranae</i> mRNA levels, indicating lower immunity and higher susceptibility. Positive correlations among AMP transcripts, especially in Caucasian, Carniolan, and Yığılca bees, suggested a coordinated response. <i>Eater</i> gene expression, critical for cellular immunity, decreased in infected Caucasian and Yığılca bees, coinciding with AMP upregulation. Vitellogenin expression, linked to immunity and longevity, increased in Carniolan and Syrian bees, correlating with higher early locomotor activity. Locomotor analysis revealed subspecies-specific behavioral responses. Syrian bees maintained the highest activity despite elevated <i>V. ceranae</i> mRNA and minimal AMP expression, suggesting unique resilience possibly mediated by vitellogenin. Muğla bees, despite high pathogen loads, exhibited decreased activity. Caucasian bees showed strong immune responses but reduced activity post-infection, reflecting potential physiological trade-offs. Overall, these findings underscore the role of genetic variability in shaping honey bee immune and behavioral responses to <i>Vairimorpha</i> and support subspecies-targeted breeding and disease management strategies to enhance resilience.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beneath the Bark and Beyond the Known: The First Record of <i>Tineobius</i> Ashmead (Hymenoptera: Chalcidoidea: Eupelmidae) in China with a Description of Two New Species.","authors":"Zixuan Li, Haoran Liao, Shirui Xu, Haitian Song, Lingfei Peng","doi":"10.3390/insects16060597","DOIUrl":"10.3390/insects16060597","url":null,"abstract":"<p><p>The genus <i>Tineobius</i> Ashmead (Hymenoptera: Eupelmidae) is recorded for the first time in China based on two new species, <i>Tineobius</i> (<i>Tineobius</i>) <i>elpisios</i> Li & Peng sp. nov. and <i>Tineobius</i> (<i>Tineobius</i>) <i>victor</i> Li & Peng sp. nov., along with two newly recorded species, <i>Tineobius (Tineobius) brachartonae</i> (Gahan, 1927) and <i>Tineobius (Tineobius) longicauda</i> (Ferrière, 1938). Descriptions and illustrations are provided for all female specimens of the four species. Additionally, the males of <i>T. victor</i> sp. nov. and <i>T. brachartonae</i> are described and illustrated in detail, and a key to Asian species based on females is provided.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}