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Formulation of Entomopathogenic Nematodes for Above-Ground Use Against Tomato Leaf Miner, Phthorimaea absoluta.
IF 2.7 2区 农林科学
Insects Pub Date : 2025-02-10 DOI: 10.3390/insects16020189
Bancy W Waweru, Joelle N Kajuga, Athanase Hategekimana, Assinapol Ndereyimana, Lydie Kankundiye, Christine Umulisa, Alphonse Nyombayire, Marie Mutumwinka, Primitive M Ishimwe, Didace Bazagwira, Grace C Mukundiyabo, Vincent de Paul Bigirimana, Xun Yan, Jozsef Kiss, Stefan Toepfer
{"title":"Formulation of Entomopathogenic Nematodes for Above-Ground Use Against Tomato Leaf Miner, <i>Phthorimaea absoluta</i>.","authors":"Bancy W Waweru, Joelle N Kajuga, Athanase Hategekimana, Assinapol Ndereyimana, Lydie Kankundiye, Christine Umulisa, Alphonse Nyombayire, Marie Mutumwinka, Primitive M Ishimwe, Didace Bazagwira, Grace C Mukundiyabo, Vincent de Paul Bigirimana, Xun Yan, Jozsef Kiss, Stefan Toepfer","doi":"10.3390/insects16020189","DOIUrl":"10.3390/insects16020189","url":null,"abstract":"<p><p>The tomato leaf miner <i>Phthorimaea</i> (syn. <i>Tuta</i>) <i>absoluta</i> Meyrick (Lepidoptera: Gelechiidae) is invasive in many agricultural regions. Its larvae feed inside leaf mines or tomato fruits and are difficult to reach with plant protection products. In contrast, entomopathogenic nematodes (EPNs) are highly virulent and can search for larvae. The challenge is to formulate EPNs to remain protected on a sprayed leaf surface until they enter the mines. We tested 11 formulations, including 2 oils, 4 gels or thickeners, 2 surfactants, 2 UV protectants, water, and combinations with and without the EPN <i>Steinernema carpocapsae</i> (Weiser) RW14-G-R3a-2 (Rhabditida: Steinernematidae), under non-closed low-humidity conditions. Six tomato leaf experiments with 1000 EPNs sprayed per leaf showed that EPNs in 0.25 to 0.5% canola oil or in 5% alkyl polyglycoside surfactant were the most effective (26 to 37%). Other formulations and an insecticide had less or no effect. Seven other bioassays showed that most formulations did not adversely affect EPN survival or pathogenicity. We assume that formulations of EPNs can be further improved, such as with other flowable gels or combinations, and encourage investment in the development of practical and economic protective sprayable formulations of EPNs against leaf miners to reduce reliance on chemical insecticides.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First Report of Polydrusus tibialis Gyllenhal (Coleoptera: Curculionidae) Infesting Peaches in Northern Greece.
IF 2.7 2区 农林科学
Insects Pub Date : 2025-02-10 DOI: 10.3390/insects16020192
Stefanos S Andreadis, Eleni I Koutsogeorgiou, Emmanouel I Navrozidis, Achilleas Kaltsidis, Dimitrios N Avtzis
{"title":"First Report of <i>Polydrusus tibialis</i> Gyllenhal (Coleoptera: Curculionidae) Infesting Peaches in Northern Greece.","authors":"Stefanos S Andreadis, Eleni I Koutsogeorgiou, Emmanouel I Navrozidis, Achilleas Kaltsidis, Dimitrios N Avtzis","doi":"10.3390/insects16020192","DOIUrl":"10.3390/insects16020192","url":null,"abstract":"<p><p>Peach [<i>Prunus persica</i> (L [...].</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856216/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pattern of Diversity and Prediction of Suitable Areas of Grasshoppers from the Qinghai-Tibet Plateau in China (Orthoptera: Acridoidea).
IF 2.7 2区 农林科学
Insects Pub Date : 2025-02-10 DOI: 10.3390/insects16020191
Bowen Bao, Xicheng Wang, Zhenrui Peng, Qingyao Zhu, Xinjiang Li, Daochuan Zhang
{"title":"Pattern of Diversity and Prediction of Suitable Areas of Grasshoppers from the Qinghai-Tibet Plateau in China (Orthoptera: Acridoidea).","authors":"Bowen Bao, Xicheng Wang, Zhenrui Peng, Qingyao Zhu, Xinjiang Li, Daochuan Zhang","doi":"10.3390/insects16020191","DOIUrl":"10.3390/insects16020191","url":null,"abstract":"<p><p>The Qinghai-Tibet Plateau is recognized as a biodiversity hotspot, with a wide variety of grasshopper species, including several endemic to the region, which play significant roles in both agricultural and forestry ecosystems. The purpose of this study was to analyze the species diversity and distribution pattern of grasshoppers on the Qinghai-Tibet Plateau. A comprehensive database comprising 390 grasshopper species was established through specimen collection, a literature review, and a geographical distribution data analysis. Diversity analysis showed that the diversity of species under the five vegetation types was relatively average. However, the alpine cold vegetation of Qinghai-Tibet and subtropical evergreen broad-leaved forest still showed a relatively high Shannon index and Simpson index. Grasshopper species are mainly concentrated in the eastern and southern parts of the Qinghai-Tibet Plateau. The richness pattern showed that grasshopper species diversity was particularly high in certain mountain areas, with Bayankala Mountain and Hengduan Mountain being endemic hotspots. The MaxEnt models were used to assess the potential habitats for four dominant genera of grasshoppers under projected climate change scenarios for 2050 and 2070. Altitude was the factor affecting the distribution of <i>Locusta</i>, <i>Chorthippus</i>, and <i>Kingdonella</i>, while precipitation and temperature were the factors affecting the distribution of <i>Leuconemacris</i>. These findings improve our understanding of the distribution patterns of different grasshopper species across various habitat types on the Qinghai-Tibet Plateau and provide valuable insights for developing targeted ecological protection strategies in response to environmental changes.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative Feeding and Defecation Behaviors of Trypanosoma cruzi-Infected and Uninfected Triatomines (Hemiptera: Reduviidae) from the Americas.
IF 2.7 2区 农林科学
Insects Pub Date : 2025-02-10 DOI: 10.3390/insects16020188
Keswick C Killets, Jillian Wormington, Italo Zecca, Luis Fernando Chaves, Gabriel L Hamer, Sarah A Hamer
{"title":"Comparative Feeding and Defecation Behaviors of <i>Trypanosoma cruzi</i>-Infected and Uninfected Triatomines (Hemiptera: Reduviidae) from the Americas.","authors":"Keswick C Killets, Jillian Wormington, Italo Zecca, Luis Fernando Chaves, Gabriel L Hamer, Sarah A Hamer","doi":"10.3390/insects16020188","DOIUrl":"10.3390/insects16020188","url":null,"abstract":"<p><p>Triatomines are vectors of <i>Trypanosoma cruzi</i> (Kinetoplastida: Trypanosomatidae), the agent of Chagas disease. Stercorarian transmission occurs when infectious parasites are passed in insect feces to vertebrates through the biting wound or mucosa. Defecating on hosts during or shortly after blood feeding is, therefore, critical for transmission, and delayed triatomine defecation behavior has been posited to contribute to a low incidence of human Chagas disease in the U.S. We allowed nymphal <i>T. cruzi</i>-infected and uninfected <i>Triatoma gerstaeckeri</i> (Stål, 1859) and <i>Triatoma sanguisuga</i> (LeConte, 1855) to interact with restrained guinea pigs and measured insect feeding and defecation events; South American <i>Rhodnius prolixus</i> (Stål, 1859; Latin America) served as a comparison group. In 148 trials, 40.0% of insects fed, of which 71.2% defecated. Compared to <i>R. prolixus</i>, <i>T. gerstaeckeri</i> had >9 times higher odds of feeding, and <i>T. sanguisuga</i> fed longer. Observations of defecation while feeding occurred across all three species. The post-feeding defecation interval (PFDI) of <i>R. prolixus</i> was significantly shorter (4.54 ± 2.46 min) than that of <i>T. gerstaeckeri</i> (9.75 ± 2.52 min) and <i>T. sanguisuga</i> (20.69 ± 8.98 min). Furthermore, the PFDI was shorter for TcI-infected insects compared to uninfected insects. <i>Triatoma gerstaeckeri</i> and <i>T. sanguisuga</i> are capable of stercorarian transmission, although the calculated metrics suggest they are less efficient vectors than <i>R. prolixus</i>.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ultrastructure and Transcriptome Analysis of the Larval Integument in Solitary and Gregarious Phases of Mythimna separata.
IF 2.7 2区 农林科学
Insects Pub Date : 2025-02-10 DOI: 10.3390/insects16020190
Lingling Li, Wenmeng Li, Jing Liao, Junhong Fu, Changgeng Dai, Yang Hu, Hongbo Li
{"title":"Ultrastructure and Transcriptome Analysis of the Larval Integument in Solitary and Gregarious Phases of <i>Mythimna separata</i>.","authors":"Lingling Li, Wenmeng Li, Jing Liao, Junhong Fu, Changgeng Dai, Yang Hu, Hongbo Li","doi":"10.3390/insects16020190","DOIUrl":"10.3390/insects16020190","url":null,"abstract":"<p><p><i>Mythimna separata</i> larvae exhibit both solitary and gregarious phases under low and high population density, respectively; furthermore, differences in morphology, behavior and physiology have been observed in the two phases. The integument plays an essential role in the fitness, general metabolism, communication, and survival of insects; however, differences in the integument ultrastructure and gene expression in the solitary and gregarious phases are largely unknown. In this study, the integument ultrastructure of larvae in the solitary and gregarious phases was compared, and transcriptome analysis was conducted to identify which genes were differentially expressed in the two phases. The results showed that the gregarious larvae had thicker integuments and more polygonal particles on the cuticle surface than solitary larvae. Using the Illumina HiSeq™ sequencing platform, 2774 differentially expressed genes (DEGs) were generated. Among these, many transcripts were identified with roles in the synthesis of fatty acids; structural components of the integument and the insecticide detoxification were differentially expressed in the integument of the two larval phases. qRT-PCR was used to validate expression patterns of the selected transcripts. This study provides a valuable resource for understanding the molecular basis of behavioral and physiological differences in the two phases of <i>M. separata</i>.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Primary Efficacy of Co-Fumigation with Methyl Bromide and Phosphine Against Tribolium castaneum and Sitophilus zeamais in Wood Pellets as a Quarantine Treatment.
IF 2.7 2区 农林科学
Insects Pub Date : 2025-02-09 DOI: 10.3390/insects16020186
Donghun Cho, Min-Goo Park
{"title":"Primary Efficacy of Co-Fumigation with Methyl Bromide and Phosphine Against <i>Tribolium castaneum</i> and <i>Sitophilus zeamais</i> in Wood Pellets as a Quarantine Treatment.","authors":"Donghun Cho, Min-Goo Park","doi":"10.3390/insects16020186","DOIUrl":"10.3390/insects16020186","url":null,"abstract":"<p><p>Pests in wood pellets are treated using methyl bromide (MB) fumigation, which has low efficacy against pests because of its high adsorption to wood pellets. In this study, we assessed the feasibility of combined MB and phosphine (PH<sub>3</sub>) fumigation of adults and pupae of <i>Tribolium castaneum</i> and <i>Sitophilus zeamais</i>. The LC<sub>99</sub> of MB for <i>T. castaneum</i> pupae were 31.49, 39.21, and 53.48 mg/L for 24 h treatment at 20, 10, and 5 °C, respectively. For <i>S. zeamais</i>, the LC<sub>99</sub> were 24.32, 36.06, and 51.49 mg/L under the same conditions. The addition of PH<sub>3</sub> (0.04 mg/L) to MB against <i>T. castaneum</i> pupae significantly decreased the LC<sub>99</sub> of MB to 4.05 mg/L at 20 °C. MB was strongly adsorbed to wood pellets, which prevented complete extermination of <i>T. castaneum</i> at loading ratios between 20 and 60%. However, at the highest filling ratio (60%), the addition of 0.7 mg/L PH<sub>3</sub> with 70 mg/L MB achieved 100% mortality for <i>T. castaneum</i>. When pupae of <i>S. zeamais</i> were targeted, MB + PH<sub>3</sub> effectively disinfected both stages of both pests after 24 h at 5, 15, and 20 °C. Combined MB + PH<sub>3</sub> treatment is an alternative to the currently approved MB treatment for controlling exotic beetles in imported wood pellets in Korea.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Modeling the Potential Climatic Suitability and Expansion Risk of Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae) Under Future Climate Scenarios.
IF 2.7 2区 农林科学
Insects Pub Date : 2025-02-09 DOI: 10.3390/insects16020185
Tai Gao, Rui Feng, Zibo Liu, Zengrong Zhu
{"title":"Modeling the Potential Climatic Suitability and Expansion Risk of <i>Tuta absoluta</i> (Meyrick, 1917) (Lepidoptera: Gelechiidae) Under Future Climate Scenarios.","authors":"Tai Gao, Rui Feng, Zibo Liu, Zengrong Zhu","doi":"10.3390/insects16020185","DOIUrl":"10.3390/insects16020185","url":null,"abstract":"<p><p>In this study, we predicted the global climatic suitability of <i>Tuta absoluta</i>, using maximum entropy (MaxEnt) modeling. We used species' natural occurrence records in 1981-2024 and environmental variables in 1981-2010, reflecting near-current climate conditions, for modeling. The occurrence records used for modeling excluded the data from greenhouses and summer-only presence. The optimized MaxEnt models demonstrated an excellent predictive performance; Jaccard's and Sørensen's indices were greater than 0.8. Temperature, particularly the mean daily air temperature in February (tas2), was identified as the primary influencing factor. Projections based on five global climate models (GCMs) and four shared socioeconomic pathways (SSPs) indicated an increasing risk of <i>T. absoluta</i> expansion. Under SSP126, the lowest-risk period (2011-2040) exhibited a 7.08% increase in suitable areas, while SSP370 during the highest-risk period (2071-2100) projected an 18.13% increase relative to near-current conditions. Model outputs underestimated the pest's actual distribution, underscoring its invasive potential. We recommend stringent quarantine measures, particularly for artificial facilities that support overwintering, to mitigate future invasions. These findings provide critical insights for policymakers and agricultural stakeholders to safeguard global tomato production against this invasive threat.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bombyx mori RPL12 Participates in UV-Induced DNA Damage Repair and Interacts with BmNPV Bm65 Protein Only After Ultraviolet Radiation.
IF 2.7 2区 农林科学
Insects Pub Date : 2025-02-09 DOI: 10.3390/insects16020187
Qi Tang, Ceru Chen, Jiaying Huang, Guohui Li, Feifei Zhu, Qian Yu, Lindan Sun, Huiqing Chen, Liang Chen, Shangshang Ma, Xiaoyong Liu, Keping Chen
{"title":"<i>Bombyx mori</i> RPL12 Participates in UV-Induced DNA Damage Repair and Interacts with BmNPV Bm65 Protein Only After Ultraviolet Radiation.","authors":"Qi Tang, Ceru Chen, Jiaying Huang, Guohui Li, Feifei Zhu, Qian Yu, Lindan Sun, Huiqing Chen, Liang Chen, Shangshang Ma, Xiaoyong Liu, Keping Chen","doi":"10.3390/insects16020187","DOIUrl":"10.3390/insects16020187","url":null,"abstract":"<p><p>Solar ultraviolet radiation (UV) may cause DNA damage. We first report in this study that the large subunit ribosome protein RPL12, from <i>Bomby mori</i> (BmRPL12), participated in UV-induced DNA damage repair. BmRPL12 enhanced the resistance of <i>Escherichia coli</i> (<i>E. coli</i>) to UV radiation and facilitated faster repair of UV-induced DNA damage in silkworm cells. BmRPL12 mainly existed in the cytoplasm in the dimer forms, and the N-terminal nuclear export signal was crucial for the localization of BmRPL12. After UV radiation, BmRPL12 was unable to localize at the UV-induced DNA damage sites to participate in damage repair directly and might indirectly regulate UV-induced DNA damage repair. Our previous research found that BmNPV Bm65 was an important UV damage-induced endonuclease. Here, it was further found that in BmNPV-infected silkworm cells, BmRPL12 in monomeric forms interacted with the virus Bm65 protein only after UV radiation, and BmRPL12 specifically localized at the UV-induced DNA damage sites only in the presence of Bm65. We speculate that after viral infection in cells subjected to UV-induced DNA damage, viral protein Bm65 interacts with BmRPL12 and localizes BmRPL12 to sites of UV-damaged DNA.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ontogenetic Analysis of Chelonus formosanus and Diversity of Its Internal Microbiota.
IF 2.7 2区 农林科学
Insects Pub Date : 2025-02-08 DOI: 10.3390/insects16020180
Jingjing Jia, Qing Feng, Weikang Huang, Zhufeng Lin, Xuncong Ji
{"title":"Ontogenetic Analysis of <i>Chelonus formosanus</i> and Diversity of Its Internal Microbiota.","authors":"Jingjing Jia, Qing Feng, Weikang Huang, Zhufeng Lin, Xuncong Ji","doi":"10.3390/insects16020180","DOIUrl":"10.3390/insects16020180","url":null,"abstract":"<p><p><i>Chelonus formosanus</i> is a parasitic wasp capable of parasitizing various Noctuidae pests, including the highly invasive <i>Spodoptera frugiperda</i>, and it demonstrates strong pest control potential. Both egg and larval stages primarily occur within the host organism, and the total developmental time from egg to adult is approximately 19.62 days. To investigate the microbial communities at different stages, we performed 16S rDNA sequencing (V1-V9 region) using PacBio sequencing and identified 404 bacterial species belonging to 61 classes, 116 orders, 182 families, and 308 genera across larval, pupal, female, and male adult stages. Bacterial diversity and richness varied across the stages, with <i>Enterobacter</i> and <i>Enterococcus</i> dominating in larvae and pupae and <i>Pseudomonas</i> emerging as dominant in female adults. In contrast, male adults predominantly resided with <i>Ralstonia</i> and <i>Achromobacter</i>. The predicted functions of bacteria within <i>C. formosanus</i> at different developmental stages are predominantly marked by the high abundance of metabolic pathways. This study provides a comprehensive understanding of the morphology of <i>C. formosanus</i> and contributes to the practical control of host pests. Additionally, our findings preliminarily characterized the microbial community of various developmental stages, laying the groundwork for its functional study.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efficacy of Chlorobenzene as a New Fumigant for Control of Confused Flour Beetle (Coleoptera: Tenebrionidae) and Rice Weevil (Coleoptera: Curculionidae).
IF 2.7 2区 农林科学
Insects Pub Date : 2025-02-08 DOI: 10.3390/insects16020183
Yong-Biao Liu
{"title":"Efficacy of Chlorobenzene as a New Fumigant for Control of Confused Flour Beetle (Coleoptera: Tenebrionidae) and Rice Weevil (Coleoptera: Curculionidae).","authors":"Yong-Biao Liu","doi":"10.3390/insects16020183","DOIUrl":"10.3390/insects16020183","url":null,"abstract":"<p><p>Chlorobenzene is an industrial chemical with relatively high vapor pressure and has been used in the past to produce pesticide DDT (Dichlorodiphenyltrichloroethane). In this study, chlorobenzene was demonstrated to be an effective fumigant against two stored product insects: the confused flour beetle (<i>Tribolium confusum</i>) and rice weevil (<i>Sitophilus oryzae</i>). In small-scale fumigations in 1.9 L glass jars, the complete control of adults of both the confused flour beetle and rice weevil was achieved in 24 h at a dose of 150 μL/L at 21 °C. LC<sub>95</sub> values of chlorobenzene vapor concentration for adults of the confused flour beetle and rice weevil were estimated to be 1121 and 1114 ppm, respectively. In large-scale fumigations in a 60 L chamber, all life stages of the confused flour beetle and rice weevil in 20 kg of corn were fumigated for 24 h with 30 mL (500 μL/L) chlorobenzene at 21 °C. The complete control of adults and immature stages of the confused flour beetle was achieved. For the rice weevil, adults had 100% mortality, and immature life stages had 97.8% mortality. These results demonstrated that chlorobenzene is effective as a fumigant against stored product insects, and it is technically feasible to conduct large-scale fumigations for postharvest pest control on stored products.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"16 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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