Insects最新文献

{"title":"Role of Gut Bacteria in Enhancing Host Adaptation of <i>Tuta absoluta</i> to Different Host Plants.","authors":"Luo-Hua Shang, Xiang-Yun Cai, Xiu-Jie Li, Yu-Zhou Wang, Jin-Da Wang, You-Ming Hou","doi":"10.3390/insects15100795","DOIUrl":"https://doi.org/10.3390/insects15100795","url":null,"abstract":"<p><p>The insect gut bacteria play important roles in insect development and growth, such as immune defense, nutrient metabolism, regulating insect adaptations for plants, etc. The <i>Tuta absoluta</i> (Meyrick) is a destructive invasive pest that mainly feeds on solanaceae plants. However, the relationship between gut microflora and host adaption of <i>T. absoluta</i> remains to be known. In this study, we first compared the survival adaptability of <i>T. absoluta</i> feeding with two host plants (tomatoes and potatoes). The <i>T. absoluta</i> completed the generation cycle by feeding on the leaves of both plants. However, the larvae feeding on tomato leaves have shorter larvae durations, longer adult durations, and a greater number of egg production per female. After Single Molecular Real-Time (SMRT) sequencing, according to the LDA Effect Size (LEfSe) analysis, the gut bacterial biomarker of <i>T. absoluta</i> fed on tomato was <i>Enterobacter cloacae</i> and the gut bacterial biomarker of <i>T. absoluta</i> fed on potatoes was <i>Staphylococcus gallinarum</i> and <i>Enterococcus gallinarum</i>. Furthermore, a total of 6 and 7 culturable bacteria were isolated from the guts of tomato- and potato-treated <i>T. absoluta</i>, respectively. However, the isolated strains included bacterial biomarkers <i>E. cloacae</i> and <i>S. gallinarum</i> but not <i>E. gallinarum</i>. In addition, different stains bacterial biomarkers on <i>T. absoluta</i> feeding selection were also studied. <i>E. cloacae</i> enhanced the host preference of the SLTA (<i>T. absoluta</i> of tomato strain) for tomato but had no impact on STTA (<i>T. absoluta</i> of potato strain). <i>S. gallinarum</i> improved the host preference of STTA to a potato but did not affect SLTA. The results showed that the gut bacteria of <i>T. absoluta</i> were affected by exposure to different host plants, and the bacterial biomarkers played an important role in host adaptability. This study not only deepens our understanding of gut bacteria-mediated insect-plant interactions but also provides theoretical support for the development of environmentally friendly and effective agricultural pest control methods.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"15 10","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11508330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-Annual Study of <i>Eriogaster catax</i> (Linnaeus, 1758) (Lepidoptera, Lasiocampidae) Oviposition Strategy in Transylvania's Largest Population: Key Insights for Species Conservation and Local Land Management.","authors":"Cristian Sitar, Geanina Magdalena Sitar, Angela Monica Ionică, Vladimír Hula, Lukáš Spitzer, Alina Simona Rusu, László Rakosy","doi":"10.3390/insects15100794","DOIUrl":"https://doi.org/10.3390/insects15100794","url":null,"abstract":"<p><p>This study provides new insights into the oviposition strategy of <i>Eriogaster catax</i> (Linnaeus, 1758) (Lepidoptera, Lasiocampidae), an endangered species of moth found in semi-natural habitats within agricultural landscapes. Protected under various European directives and listed as Data Deficient by the IUCN, <i>E. catax</i> inhabits warmer regions of the Western Palearctic. Despite noted geographic variations in its ecological preferences, few studies have statistically significant data on its ecology. Our six-year study, conducted within the largest known population of <i>E. catax</i>. in Romania, reveals critical data on its oviposition preferences, including the species' tendency to utilize <i>Prunus spinosa</i> L. and <i>Crataegus monogyna</i> Jacq. shrubs at an average height of 80.48 ± 34.3 cm, with most nests placed within the 41-80 cm range and containing an average of 186 ± 22 eggs. The study also addresses the species' vulnerability to human activities such as bush trimming, agricultural burning, and uncontrolled grazing, particularly due to its low oviposition height. These findings underscore the negative impact of overgrazing and burning practices, particularly when conducted on a large scale, on the conservation of <i>E. catax.</i> The detailed ecological requirements identified in this study are essential for developing effective conservation strategies and habitat management practices. Furthermore, the study highlights the importance of local community involvement and public education in raising awareness about biodiversity and the conservation of endangered species.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"15 10","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11508962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Aggregation Pheromone Traps on Spatial Distribution of <i>Halyomorpha halys</i> Damage in Apple Orchards.","authors":"Veronica Carnio, Riccardo Favaro, Michele Preti, Sergio Angeli","doi":"10.3390/insects15100791","DOIUrl":"https://doi.org/10.3390/insects15100791","url":null,"abstract":"<p><p><i>Halyomorpha halys</i> (Stål) (Hemiptera: Pentatomidae) is an invasive pest causing significant damage to tree crops. Our study examined the impact of newly designed aggregation pheromone-baited 'mini-sailboat' (MSB) traps for controlling <i>H. halys</i> and its effect on the spatial distribution of fruit damage. Four replicates of four traps, with a total of 16 MSB traps, were placed along a 1.3 km border of apple orchards, concentrating the traps on one side of the orchards. A fruit damage assessment for incidence and severity was conducted at increasing distances from the orchard border where the traps were placed, encompassing 107 assessment points. Our study showed that deploying MSB traps along the orchard border significantly increased fruit damage within the first 45 m compared to control plots without traps. However, beyond the first 45 m from the border, there was a significant reduction in damage incidence. In the treated plots, 50% of the damage occurred within 26 m of the traps, while in control plots, within 85 m. Shifting the fruit damage pattern means restricting the pests lingering in a narrow strip near the MSB traps, which paves the way for improved techniques to restructure the crop perimeter.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"15 10","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11509157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Molecular Phylogenetic Evidence Confirms Independent Origin of Coxal Combs in the Families of the 'Cydnoid' Complex (Hemiptera: Heteroptera: Pentatomoidea).","authors":"Jerzy A Lis, Paweł J Domagała, Barbara Lis","doi":"10.3390/insects15100792","DOIUrl":"https://doi.org/10.3390/insects15100792","url":null,"abstract":"<p><p>Coxal combs, found only in members of the 'cydnoid' complex (comprising four families: Cydnidae, Parastrachiidae, Thaumastellidae, and Thyreocoridae) within the superfamily Pentatomoidea, have long been regarded as a character confirming their close evolutionary relationship. However, many studies have demonstrated that these four families are phylogenetically distant. Others have been treated as subfamilies of the broadly defined Cydnidae, with the coxal combs as the only character linking them. This is the first study on the origin of coxal combs in species of all families and subfamilies that represent the broadly conceived Cydnidae (69 species in 39 genera). Moreover, this study presents the first 16S rDNA gene sequences providing a basis for such analyses. The analyses included DNA sequences of 62 species in 34 genera of Cydnidae <i>sensu stricto</i>, three species in two genera of Thyreocoridae, two species in two genera of Parastrachiidae, and two species in one genus of Thaumastellidae. The sequence analysis in the family Cydnidae covered 35 species representing 19 genera of the subfamily Cydninae, 16 species in eight genera of the subfamily Sehirinae, five species in two genera of Amnestinae, three species in three genera of Garsauriinae, two species in one genus of Cephalocteinae, and one species of Amaurocorinae. The results of our study demonstrate the independent origin of coxal combs in taxa of the 'cydnoid' complex within the superfamily Pentatomoidea. They confirm the polyphyly not only of the entire 'cydnoid' complex but also of the family Cydnidae itself.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"15 10","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11509079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Transcriptome Analysis of Cold Tolerance Mechanism in Honeybees (<i>Apis mellifera sinisxinyuan</i>).","authors":"Jinqiong Shan, Ruiyi Cheng, Tuohudasheng Magaoya, Yujie Duan, Chao Chen","doi":"10.3390/insects15100790","DOIUrl":"https://doi.org/10.3390/insects15100790","url":null,"abstract":"<p><p>Honeybees are important pollinators worldwide that are closely related to agricultural production and ecological balance. The biological activities and geographical distribution of honeybees are strongly influenced by temperature. However, there is not much research on the cold tolerance of honeybees. The <i>Apis mellifera sinisxinyuan</i>, a kind of western honeybee, exhibits strong cold hardiness. Here, we determined that short-term temperature treatment could regulate the honeybee's cold tolerance ability by measuring the supercooling point of <i>A. m. sinisxinyuan</i> treated with different temperatures. Transcriptome data were analyzed between the treated and untreated honeybees. A total of 189 differentially expressed genes were identified. Among them, <i>Abra</i>, <i>Pla1</i>, <i>rGC</i>, <i>Hr38</i>, and <i>Maf</i> were differentially expressed in all comparisons. GO and KEGG analysis showed that the DEGs were enriched in molecular functions related to disease, signal transduction, metabolism, and the endocrine system's function. The main components involved were ribosomes, nucleosomes, proteases, and phosphokinases, among others. This study explored the formation and regulation mechanism of cold tolerance in honeybees, not only providing a theoretical basis for cultivating honeybees with excellent traits but also promoting research and practice on insect stress tolerance.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"15 10","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11508713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Buzzing towards Resilience: Investigating the Spatial Alignment of the Desert Pallid Bee, <i>Centris pallida</i>, and Its Host Plants in Response to Climate Change.","authors":"Terese Maxine P Cruz, Stephen L Buchmann, Kathleen L Prudic","doi":"10.3390/insects15100793","DOIUrl":"https://doi.org/10.3390/insects15100793","url":null,"abstract":"<p><p>Wild bees are vital for the pollination of native plants and crops, providing essential ecosystem services. Climate change is known to impact biodiversity and species distributions, but insects adapted to desert ecosystems may exhibit unique physiological, behavioral, and evolutionary responses. The desert pallid bee (<i>C. pallida</i>), a solitary bee native to the arid southwestern United States and northern Mexico, primarily forages on yellow palo verde (<i>P. microphylla</i>), blue palo verde (<i>P. florida</i>), and desert ironwood (<i>O. tesota</i>). This study used MaxEnt to estimate the current and projected geographical overlap of suitable habitats for <i>C. pallida</i> and its host plants. Here, we used MaxEnt to estimate the current and forecasted overlapping geographically suitable habitat of <i>C. pallida</i> with all three host plants. We forecasted potential environmentally suitable areas for each species to the year 2040 using the current distribution model and climate projections with moderate CO₂ levels. We found a continued spatial alignment in the suitable area of the bee and its host plants with a 70% increase in the range overlap area, though shifted to higher average altitudes and a slight northern expansion. These findings may provide insight to stakeholders on the conservation needs of desert-dwelling pollinators.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"15 10","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11508805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feeding and Growth Response of Fall Armyworm <b><i>Spodoptera frugiperda</i> (Lepidoptera: Noctuidae)</b> towards Different Host Plants.","authors":"Muhammad Saqib Ajmal, Sajjad Ali, Aftab Jamal, Muhammad Farhan Saeed, Emanuele Radicetti, Stefano Civolani","doi":"10.3390/insects15100789","DOIUrl":"https://doi.org/10.3390/insects15100789","url":null,"abstract":"<p><p>The fall armyworm, <i>Spodoptera frugiperda</i>, is a major migratory polyphagous insect pest of various crops. The essential nutrient and mineral profile of the host plants determines the feeding fitness of herbivorous insects. As a result, the growth and development of insects is affected. To determine the effect of the nutrient and mineral profile of different host plants (maize, castor bean, cotton, cabbage, okra, and sugarcane) on the growth and development of <i>S. frugiperda</i>, biological parameters like larval weight, pupal weight (male/female), and feeding and growth indices were calculated. The proximate compositions such as crude protein, crude fat, crude fibre, and ash and mineral contents of the tested host plants showed significant differences (<i>p</i> < 0.05). The feeding indices on these host plants also differed significantly (<i>p</i> < 0.05). The maximum relative growth rate (RGR), relative consumption rate (RCR), and consumption index (CI) were recorded in <i>S. frugiperda</i> larvae that fed on maize and castor bean leaves. The crude protein, dry matter, and ash contents in maize and castor bean were significantly higher and positively correlated with the RGR and RCR of <i>S. frugiperda</i> larvae. The larval, male and female pupal weights were the maximum in the larvae feeding on the castor bean host plant. These findings provide novel information based on nutritional ecology to develop sustainable integrated pest management strategies using selective crop rotation.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"15 10","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11508452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Diversity of Arachnid Assemblages on the Endemic Tree <i>Zelkova abelicea</i> (Ulmaceae): An Evaluation of Fragmentation and Connectivity in Crete (Greece).","authors":"Dariusz J Gwiazdowicz, Laurence Fazan, Giulio Gardini, Dany Ghosn, Sławomir Kaczmarek, Alireza Nemati, Ilektra Remoundou, Tomasz Rutkowski, Piotr Skubała, Bogna Zawieja, Gregor Kozlowski","doi":"10.3390/insects15100788","DOIUrl":"https://doi.org/10.3390/insects15100788","url":null,"abstract":"<p><p><i>Zelkova abelicea</i> is an endemic tree growing only on eight mountain stands on the Greek island of Crete. The aim of this study was to determine the structure of the assemblages and analyze the diversity of the arachnid assemblages living on <i>Zelkova abelicea</i>, an endemic tree species in Crete. Material for the analyses was collected from tree trunks, oftentimes covered by bryophytes or lichens. In the examined material, 85 taxa were recorded. The most numerous groups represented in the analyzed material were Acari, including representatives of the orders Mesostigmata (78 ind. of 18 spp.) and Oribatida (1056 ind. of 51 spp.). In the order Mesostigmata the species represented by the highest numbers of specimens were <i>Onchodellus karawaiewi</i> (15 individuals) and <i>Hypoaspisella</i> sp. (13), which is probably a species new to science. In turn, representatives of the order Oribatida were much more numerous, with <i>Zygoribatula exilis</i> (284) and <i>Eremaeus tuberosus</i> (210) being identified in the largest numbers. Among the eight sampled localities, Gerakari (646 ind. and 50 spp.) and Omalos (409 ind. and 43 spp., respectively) had by far the richest assemblages. Statistical analyses confirmed the highly diverse character of the arachnid assemblages at the individual sites, which is a consequence not only of the varied numbers of arachnids found, but also of the presence of very rare species, such as <i>Androlaelaps shealsi</i>, <i>Cosmolaelaps lutegiensis</i> or <i>Hoploseius oblongus</i>. These results highlight the high species diversity of the arachnids found on <i>Z. abelicea</i> but also suggest the lack of connectivity between the isolated and fragmented forest stands on Crete.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"15 10","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11508438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Megaselia scalaris</i> and <i>Senotainia tricuspis</i> Infesting <i>Apis mellifera</i>: Detection by Quantitative PCR, Genotyping, and Involvement in the Transmission of Microbial Pathogens.","authors":"Franca Rossi, Martina Iannitto, Beqe Hulaj, Paola Manocchio, Francesca Gentile, Ilaria Del Matto, Massimiliano Paoletti, Lucio Marino, Luciano Ricchiuti","doi":"10.3390/insects15100786","DOIUrl":"https://doi.org/10.3390/insects15100786","url":null,"abstract":"<p><p>The <i>Megaselia scalaris</i> and <i>Senotainia tricuspis</i> parasitoid flies of the honeybee <i>Apis mellifera</i> were found to infest apiaries of different European and Mediterranean countries but their prevalence and impact on apiary health are little known. Therefore, in this study, quantitative PCR (qPCR)-based methods were developed for their rapid detection directly in hive matrices. The newly developed qPCR assays were targeted at the mitochondrial cytochrome oxidase subunit I (<i>COI</i>) gene for the <i>M. scalaris</i> and the cytochrome B (<i>cyt</i>B) gene for the <i>S. tricuspis.</i> The tests were preliminarily applied to 64 samples of adult honeybees and hive debris collected in the Abruzzo and Molise regions, Central Italy, and the Republic of Kosovo showing that both flies occur in the two countries and more frequently in Italy. The positive apiaries in Italy were re-sampled by capturing viable forager bees and isolating emerging flies to carry out the genotyping and analyses aimed at defining if these flies can transmit honeybee pathogens. Genotyping based on the <i>COI</i> and <i>cyt</i>B gene sequencing for <i>M. scalaris</i> and <i>S. tricuspis</i>, respectively, identified one <i>S. tricuspis</i> genotype and diverse genotypes of <i>M. scalaris</i> highly similar to those from distant countries. Some fly isolates harbored the DNA or RNA of honeybee microbial pathogens <i>Paenibacillus larvae</i>, deformed wing viruses A and B (DWVA and B), black queen cell virus (BQCV), chronic paralysis virus (CBPV), and <i>Nosema ceranae</i>. The results indicated that these parasites should be efficiently controlled in apiaries by using rapid detection methods to facilitate the large screening studies and early detection.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"15 10","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11508623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142499662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"No Evidence for <i>Wolbachia</i> Effects on the Thermal Preference of the Invasive Pest <i>Liriomyza huidobrensis</i>.","authors":"Yuxi Zhu, Xinyu Wang, Sibo Wang, Zhangrong Song, Yuzhou Du","doi":"10.3390/insects15100784","DOIUrl":"https://doi.org/10.3390/insects15100784","url":null,"abstract":"<p><p>Heritable endosymbiont <i>Wolbachia</i> is prevalent among arthropods, serving multiple functions for their hosts. However, the role of <i>Wolbachia</i> in mediating thermal preference selection remains largely unexplored. In this study, we utilized a custom-built thermal gradient to evaluate the thermal preference (Tp) of 1367 individuals of the invasive leaf-miner <i>Liriomyza huidobrensis</i> with or without <i>Wolbachia</i> wLhui from Yunnan and Xinjiang populations. Under meticulously controlled conditions and with a vast sample size, we found no significant difference in the mean Tp between wLhui-infected and uninfected leaf miners from either population when host age and sex were not considered. Furthermore, generalized linear model (GLM) analysis revealed no significant correlation between average Tp and age, sex, or <i>Wolbachia</i> infection, nor interactions among these factors, except in the Xinjiang population, where Tp was strongly associated with host age. Finally, we discuss the ecological implications of these findings and propose future research directions on <i>Wolbachia</i>-mediated host Tp in the leaf miner. Overall, our findings do not provide evidence that <i>Wolbachia</i> significantly affects the thermal preference of <i>L. huidobrensis</i>. Further studies across different systems are needed to investigate the complex interactions between <i>Wolbachia</i> and insect thermal behavior.</p>","PeriodicalId":13642,"journal":{"name":"Insects","volume":"15 10","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11508833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142516383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}