Infectious Agents and Cancer最新文献

{"title":"From pathogen to cure: exploring the antitumor potential of Toxoplasma gondii.","authors":"Parisa Alipanahi, Arezou Khosrojerdi, Abdol Satar Pagheh, Kareem Hatam-Nahavandi, Ehsan Ahmadpour","doi":"10.1186/s13027-025-00673-z","DOIUrl":"10.1186/s13027-025-00673-z","url":null,"abstract":"","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"39"},"PeriodicalIF":3.1,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12175340/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skin biopsy processing for rapid molecular diagnosis and histopathologic interpretation: application to Kaposi sarcoma in East Africa.","authors":"Jason C Manning, Xinying Chu, Juan Boza, Racheal Ayanga, Hilda Muwando, Robert Lukande, Marcelo Horenstein, Toby Maurer, Ethel Cesarman, Aggrey Semeere, Jeffrey Martin, David Erickson","doi":"10.1186/s13027-025-00671-1","DOIUrl":"10.1186/s13027-025-00671-1","url":null,"abstract":"<p><strong>Background: </strong>Kaposi sarcoma (KS) is a cancer of viral origin (Kaposi sarcoma-associated herpesvirus; KSHV) for which the detection of KSHV DNA is an attractive target for a rapid, automatable diagnostic test. We previously demonstrated favorable diagnostic accuracy using loop-mediated isothermal amplification (LAMP) to quantitate KSHV DNA in lesional skin biopsies, though extracting DNA from the punch biopsies was the time-limiting step. Herein, we describe the development of a biopsy processing tool called Slicer to enable rapid nucleic acid testing in addition to traditional histopathological interpretation.</p><p><strong>Methods: </strong>Slicer divides skin punch biopsies into two ½-cylinders and a thin, cross-sectional slice. The thin slice enables a previously demonstrated, equipment-free alkaline extraction termed ColdSHOT while the remaining ½-cylinders are available for histopathological diagnosis and additional molecular testing as needed. Slicer prototypes were used on skin punch biopsies collected from patients in Uganda who were referred for clinical suspicion of KS.</p><p><strong>Results: </strong>For 27 patient samples, the combination of Slicer and ColdSHOT sample processing with LAMP testing resulted in qualitative KSHV DNA detection that was fully concordant with US-based histopathological diagnoses. Additional analysis demonstrated compatibility of Slicer and ColdSHOT with qPCR for KSHV DNA quantitation.</p><p><strong>Conclusions: </strong>These results warrant further investigation using a larger set of skin biopsies and indicate that the Slicer and ColdSHOT could enable accurate KS diagnosis within a few hours of biopsy collection with minimal equipment.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"38"},"PeriodicalIF":3.1,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12175338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144316835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mendelian randomization in cancer research: opportunities and challenges.","authors":"Mengyao Tang, Lanlan Chen","doi":"10.1186/s13027-025-00672-0","DOIUrl":"10.1186/s13027-025-00672-0","url":null,"abstract":"<p><p>Mendelian Randomization (MR) is increasingly used in cancer research to infer causal relationships by leveraging genetic variants as instrumental variables. While the growth of genome-wide association studies and biobank data has expanded the utility of MR, this surge-particularly pronounced in China-raises concerns about methodological rigor. The widespread adoption may be partly driven by the Chinese translation of key MR literature. Recent advances such as multivariable MR, mediation analysis, and integration with AI and omics data have enhanced the robustness and biological interpretability of MR studies. However, challenges persist, including horizontal pleiotropy, weak instrument bias, and misinterpretation of biomarkers as causal exposures. To improve MR study credibility, frameworks like STROBE-MR and MR-GRADE are being adopted. This article reviews methodological improvements and persistent pitfalls in MR, especially within cancer epidemiology, and highlights strategies for ensuring validity in this rapidly evolving field.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"37"},"PeriodicalIF":3.1,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12168377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144301987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA hypomethylation modification promotes BST2 expression in cervical cancer by facilitating STAT1 binding to the promoter of BST2.","authors":"Reziwanguli Wubuli, Zumurelaiti Ainiwaer, Mayinuer Niyazi, Lili Han","doi":"10.1186/s13027-025-00670-2","DOIUrl":"10.1186/s13027-025-00670-2","url":null,"abstract":"<p><p>Cervical cancer (CC) is a common cancer that causes considerable morbidity and mortality, especially in developing countries. Bone marrow stromal cell antigen 2 (BST2) is a transmembrane glycoprotein, and its promoter methylation has been extensively documented in numerous human cancers. Nevertheless, the specific role of BST2 in CC remains unclear. This research utilized methylation-specific PCR (MSP), Western blotting, and RT-qPCR to evaluate the expression and DNA methylation levels of BST2 in CC tissues and cells. The role of STAT1 in regulating BST2 transcription was confirmed through dual-luciferase reporter assays and chromatin immunoprecipitation (ChIP) assays. Furthermore, we conducted experiments on cell proliferation, apoptosis, epithelial-mesenchymal transition (EMT), and xenograft tumor models to investigate the functional role and regulatory mechanisms of BST2 in CC, both in vitro and in vivo. We found that BST2 was increased in CC tissues and cells, promoting cell proliferation and EMT while inhibiting apoptosis. Mechanistically, BST2 upregulation was associated with hypomethylation of its promoter, potentially regulated by DNMT3a and DNMT3b. Furthermore, the transcription factor STAT1 was found to bind to the BST2 promoter, positively regulating its expression and thereby accelerating tumorigenesis in CC. Silencing BST2 significantly reduced tumor growth in vivo. Our findings highlight BST2 as a potential biomarker and therapeutic target in CC, with its expression regulated by DNA methylation and STAT1 binding.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"36"},"PeriodicalIF":3.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12166601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic analysis of inconsistent combinations of HPV and p16 in a Chinese/Asian oropharyngeal squamous cell carcinoma population.","authors":"Jieying Li, Kai Zhou, Xiaohong Zhan, Haijun Lu, Dapeng Hao, Kai Song, Shuangyi Wang, Yuanyong Feng, Haoyue Xu, Zongxuan He, Xiaochen Yang, Wei Shang, Lin Wang","doi":"10.1186/s13027-025-00657-z","DOIUrl":"10.1186/s13027-025-00657-z","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study was to investigate the effect of inconsistent expression of p16 and HPV on the prognosis of patients with Oropharyngeal squamous cell carcinoma (OPSCC) in Chinese/Asian populations.</p><p><strong>Methods: </strong>The study included 130 patients. Inclusion criteria were primary OPSCC. The primary outcome was the proportion of cohort patients showing different combinations of p16 and HPV outcomes, as well as overall survival (OS) and progression-free survival (PFS). Patients with relapsed or metastatic disease or palliative care were excluded from the survival analysis. A multivariate analysis model was used to calculate the adjusted hazard ratio for overall survival for different p16 and HPV tests, adjusted for pre-specified confounders.</p><p><strong>Results: </strong>Among the 130 patients, 25 (19.2%) demonstrated inconsistency between HPV and p16 expressions. The inconsistency in HPV/p16 status was significantly associated with patient age, smoking history, and alcohol consumption, leading to significant differences in tumor site, TNM staging, and differentiation, thereby influencing treatment decisions. There were significant differences in OS (P = 0.04) and PFS (P = 0.011) among the three groups, with the inconsistent group falling between the HPV+/p16 + group and the HPV-/p16- group but closer to the latter. Out of 54 p16-positive patients, only 33 (61.1%) were HPV-positive, indicating a lower predictive value of p16 for HPV positivity in OPSCC than observed in Western populations. Moreover, the study suggested a potential positive correlation between p16 expression intensity and improved patient prognosis.</p><p><strong>Conclusion: </strong>In the China/Asia region, where HPV infection rates are relatively low, the predictive power of p16 for HPV-related OPSCC is low. We recommend additional HPV testing in patients with p16 + OPSCC to improve diagnostic accuracy, thereby enabling the selection of the best de-escalation treatment strategy, increasing treatment response rates, and ultimately improving the overall prognosis in these patients.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"35"},"PeriodicalIF":3.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12150553/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-risk HPV genotypes in women with abnormal cytology: a 12-year retrospective study.","authors":"Masoumeh Aslanimehr, Shabnam Nemati, Hamid Sadeghi, Fatemeh Samiee-Rad, Sahand Ghafari, Taghi Naserpour-Farivar","doi":"10.1186/s13027-025-00664-0","DOIUrl":"10.1186/s13027-025-00664-0","url":null,"abstract":"<p><strong>Background and aim: </strong>Persistent infections with high-risk human papillomavirus (HR-HPV) are linked to cervical cancer progression. The prevalence and distribution of HPV genotypes vary across regions and lesion severity. Comprehensive data on HPV genotype distribution among Iranian women is limited. This study investigates the distribution of HR-HPV genotypes in women with abnormal cytology in Qazvin province, northwest Iran, from 2007 to 2019.</p><p><strong>Materials and methods: </strong>A total of 103 samples, including benign cases, Low-grade Squamous Intraepithelial Lesions (LSIL), High-grade Squamous Intraepithelial Lesions (HSIL), and Invasive Cervical Cancer (ICC), were analyzed using real-time PCR to detect HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 58, and 59.</p><p><strong>Results: </strong>The study revealed a high HPV prevalence (92.23%), with HPV-16 being the most common genotype (66.31%), followed by HPV-45 (49.47%), HPV-33 (41.05%), HPV-31(30.52%) and HPV-52 (23.15%). HPV-18 was detected only in 3 (3.15%) of cases. Of the HPV-positive samples, 82.11% had multiple infections, with HPV-16, HPV-33, and HPV-45 more prevalent in these cases. HPV-16 was significantly associated with severe lesions, particularly in ICC cases (92%, P = 0.007).</p><p><strong>Conclusion: </strong>These findings emphasize the role of HPV genotyping in assessing cervical lesion severity and oncogenic risk, highlighting HPV-16 as the dominant genotype across various lesion grades. The study suggests that HPV-33 and HPV-45 may also contribute significantly to cervical lesion progression.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"34"},"PeriodicalIF":3.1,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12105159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HTLV-1 infection and microRNAs: unraveling the complex crosstalk.","authors":"Fahime Edalat, Arash Letafati, Tanin Kaghazchi, Mahdieh Sadeghi, Ali Taheri, Alireza Shikki, Samira Hossein Garkani, Mehdi Afrozi, Mehdi Norouzi, Sayed-Hamidreza Mozhgani","doi":"10.1186/s13027-025-00658-y","DOIUrl":"10.1186/s13027-025-00658-y","url":null,"abstract":"<p><p>Human T-cell leukemia virus type 1 (HTLV-1) retrovirus that infects millions of individuals worldwide, have caused severe diseases like adult T-cell leukemia/lymphoma (ATLL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Despite extensive research efforts, the underlying mechanisms leading to HTLV-1 pathogenesis remain incompletely understood. New research has revealed that microRNAs (miRNAs) play a crucial role in the complex interplay between HTLV-1 infection and host cellular responses. This review highlights the multifaceted interactions between HTLV-1 and miRNAs, encompassing both viral manipulation of cellular miRNA networks and host miRNA-mediated responses. Gaining a comprehensive understanding of the complex interconnection between HTLV-1 infection and miRNAs provides a significant opportunity for discovering innovative therapeutic approaches and creating advanced diagnostic tools aimed in HTLV-1 treatment.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"33"},"PeriodicalIF":3.1,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12100943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiota and metabolite profiles of saliva, oral swab and cancer tissue from patients with oral squamous cell carcinoma (OSCC).","authors":"Kailiu Wu, Beihui Xu, Xinyu Zhou, Haiyan Guo, Guanhuan Du, Chenping Zhang, Fuxiang Chen, Xu Chen","doi":"10.1186/s13027-025-00662-2","DOIUrl":"10.1186/s13027-025-00662-2","url":null,"abstract":"<p><p>Oral squamous cell carcinoma (OSCC) was the most common malignant type of head and neck squamous cell carcinoma (HNSCC) with a low survival rate. The microbiota in oral cavity or tumor tissues may play a critical role in the OSCC. In this study, we characterized the microbiota from oral cancer tissues, oral swabs and saliva of patients with OSCC using 16S rRNA sequencing. We found differential profiles and amounts of microbiota in oral cancer tissues compared with adjacent tissues, as well as in oral swabs and saliva from OSCC patients compared with healthy individuals. Fusobacterium nucleatum and Porphyromonas endodontalis were found increased in cancer tissues and saliva from OSCC patients. Prevotella melaninogenica was found increased in the saliva and oral swabs from OSCC patients. These data suggested that microbiota varied according to different samples. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated an important role of metabolic pathways in the interaction between microbiota and cancers. Then we analyzed the metabolites from cancer tissues and saliva of OSCC patients by liquid chromatograph-mass spectrometry/mass spectrometry (LC-MS/MS) and gas chromatograph-mass spectrometry (GC-MS). Differential profiles of metabolites were also observed in the cancer tissues compared with adjacent tissues and in the saliva from OSCC patients compared with healthy individuals. It showed that denticulaflavonol was significantly increased while D-mannose was significantly decreased in both cancer tissues and saliva of OSCC patients. Taken together, these results suggested an association between microbiota/metabolites (such as Fusobacterium and mannose) and OSCC, in which the molecular mechanism need further investigated.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"32"},"PeriodicalIF":3.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12100927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of divergent gene expression between HPV + and HPV- head and neck squamous cell carcinoma patients.","authors":"Kasturika Shankar, Sarah E Walker","doi":"10.1186/s13027-025-00663-1","DOIUrl":"10.1186/s13027-025-00663-1","url":null,"abstract":"<p><p>Human Papillomavirus (HPV) is a non-enveloped virus with a circular double-stranded DNA genome. It is one of the most common sexually transmitted infections, with high-risk types such as HPV-16 and HPV-18 linked to anogenital and head and neck squamous cell carcinomas (HNSCC). HNSCC includes cancers of the oral cavity, pharynx, larynx, and related regions, caused by carcinogens or persistent viral infections. HPV-positive (HPV+) HNSCC cases are more prevalent in Western countries and exhibit better prognosis and treatment response compared to HPV-negative (HPV-) cases. These differences suggest distinct fundamental differences between each subtype. This study analyzed RNA-seq data from the PanCancer Atlas 2018 dataset to investigate molecular distinctions between HPV + and HPV- HNSCC. Using dimensionality reduction techniques such as Principal Component Analysis (PCA) and Uniform Manifold Approximation and Projection (UMAP), a clear clustering of HPV + cases was observed, suggesting a unique gene expression profile. HPV + tumors exhibited upregulation of genes involved in nucleic acid processing and downregulation of genes associated with apoptosis and epidermis development. These findings underscore the biological differences between HPV + and HPV- HNSCC, offering insights into HPV-driven oncogenesis. Understanding these distinctions may improve patient stratification and inform targeted therapeutic strategies for HNSCC.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"31"},"PeriodicalIF":3.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma Epstein-Barr virus DNA for the prediction of treatment response and disease progression in non-keratinizing differentiated nasopharyngeal carcinoma.","authors":"Guan-Zhong Lu, Yun-Xia Huang, Lin-Feng Guo, Yi-Feng Yu, Zhen-Zhen Lu, Qin Lin, San-Gang Wu","doi":"10.1186/s13027-025-00661-3","DOIUrl":"10.1186/s13027-025-00661-3","url":null,"abstract":"<p><strong>Purpose: </strong>To explore the failure patterns, outcomes, and treatment response of differentiated non-keratinizing nasopharyngeal carcinoma (DNKC) and to further investigate the role of plasma Epstein-Barr virus (EBV)-DNA in follow-up monitoring, prognostic prediction, and assessment of treatment efficacy in DNKC.</p><p><strong>Methods: </strong>We retrospectively collected data from patients diagnosed with DNKC from January 2015 to February 2022. The life-table method, Kaplan-Meier survival, and Cox proportional hazards analysis were used for statistical analyses.</p><p><strong>Results: </strong>A total of 102 patients were included. Of the 77 patients with available EBV-DNA levels, 61 patients (79.2%) had EBV-DNA detectable before treatment. Twenty-seven patients (26.5%) experienced disease recurrence, and 88.9% (24/27) relapsed in the first three years. There were 20 patients who experienced disease recurrence and had pre-treatment EBV-DNA status records. At the time of disease progression, 4 patients initially had undetectable EBV-DNA remained undetectable. Among the 16 patients with initially detectable EBV-DNA, 15 (93.8%) had detectable EBV-DNA. Nodal stage and EBV-DNA levels before treatment were found to be independent prognostic factors for distant metastasis-free survival (DMFS) and disease-free survival (DFS). Those with residual EBV-DNA after induction chemotherapy had significantly inferior DMFS (P = 0.003), DFS (P = 0.006), and overall survival (OS) (P = 0.006) than those without residual EBV-DNA after IC. Those with residual EBV-DNA after radiotherapy had significantly inferior local recurrence-free survival (P = 0.003), DMFS (P < 0.001), DFS (P < 0.001), OS (P < 0.006) than those without residual EBV-DNA after radiotherapy.</p><p><strong>Conclusion: </strong>Our study highlights the aggressive nature of DNKC, characterized by early recurrence. EBV-DNA levels may serve as a biomarker to monitor treatment response, prognostic prediction, and recurrence surveillance.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"30"},"PeriodicalIF":3.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12090694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}