High-risk HPV genotypes in women with abnormal cytology: a 12-year retrospective study.

IF 2.8 2区医学 Q3 IMMUNOLOGY

Infectious Agents and Cancer Pub Date : 2025-05-26 DOI:10.1186/s13027-025-00664-0

Masoumeh Aslanimehr, Shabnam Nemati, Hamid Sadeghi, Fatemeh Samiee-Rad, Sahand Ghafari, Taghi Naserpour-Farivar

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引用次数: 0

Abstract

Background and aim: Persistent infections with high-risk human papillomavirus (HR-HPV) are linked to cervical cancer progression. The prevalence and distribution of HPV genotypes vary across regions and lesion severity. Comprehensive data on HPV genotype distribution among Iranian women is limited. This study investigates the distribution of HR-HPV genotypes in women with abnormal cytology in Qazvin province, northwest Iran, from 2007 to 2019.

Materials and methods: A total of 103 samples, including benign cases, Low-grade Squamous Intraepithelial Lesions (LSIL), High-grade Squamous Intraepithelial Lesions (HSIL), and Invasive Cervical Cancer (ICC), were analyzed using real-time PCR to detect HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 58, and 59.

Results: The study revealed a high HPV prevalence (92.23%), with HPV-16 being the most common genotype (66.31%), followed by HPV-45 (49.47%), HPV-33 (41.05%), HPV-31(30.52%) and HPV-52 (23.15%). HPV-18 was detected only in 3 (3.15%) of cases. Of the HPV-positive samples, 82.11% had multiple infections, with HPV-16, HPV-33, and HPV-45 more prevalent in these cases. HPV-16 was significantly associated with severe lesions, particularly in ICC cases (92%, P = 0.007).

Conclusion: These findings emphasize the role of HPV genotyping in assessing cervical lesion severity and oncogenic risk, highlighting HPV-16 as the dominant genotype across various lesion grades. The study suggests that HPV-33 and HPV-45 may also contribute significantly to cervical lesion progression.

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细胞学异常女性的高危HPV基因型：一项12年回顾性研究

背景和目的：持续感染高危人乳头瘤病毒（HR-HPV）与宫颈癌进展有关。HPV基因型的患病率和分布因地区和病变严重程度而异。伊朗妇女中HPV基因型分布的综合数据有限。本研究调查了2007 - 2019年伊朗西北部加兹温省细胞学异常妇女中HR-HPV基因型的分布。材料与方法：采用实时荧光定量PCR检测良性病例、低级别鳞状上皮内病变（Low-grade Squamous Intraepithelial lesion， LSIL）、高级别鳞状上皮内病变（High-grade Squamous Intraepithelial lesion， HSIL）和侵袭性宫颈癌（Invasive Cervical Cancer， ICC）共103例，检测HPV 16、18、31、33、35、39、45、51、52、58和59型。结果：该地区HPV患病率较高（92.23%），以HPV-16型最常见（66.31%），其次为HPV-45型（49.47%）、HPV-33型（41.05%）、HPV-31型（30.52%）和HPV-52型（23.15%）。HPV-18仅检出3例（3.15%）。在hpv阳性样本中，82.11%有多重感染，HPV-16、HPV-33和HPV-45在这些病例中更为普遍。HPV-16与严重病变显著相关，特别是在ICC病例中（92%,P = 0.007）。结论：这些发现强调了HPV基因分型在评估宫颈病变严重程度和致癌风险中的作用，强调HPV-16是各种病变等级的优势基因型。该研究表明HPV-33和HPV-45也可能对宫颈病变的进展起重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Infectious Agents and Cancer ONCOLOGY-IMMUNOLOGY

CiteScore

5.80

自引率

2.70%

发文量

期刊介绍： Infectious Agents and Cancer is an open access, peer-reviewed online journal that encompasses all aspects of basic, clinical, epidemiological and translational research providing an insight into the association between chronic infections and cancer. The journal welcomes submissions in the pathogen-related cancer areas and other related topics, in particular: • HPV and anogenital cancers, as well as head and neck cancers; • EBV and Burkitt lymphoma; • HCV/HBV and hepatocellular carcinoma as well as lymphoproliferative diseases; • HHV8 and Kaposi sarcoma; • HTLV and leukemia; • Cancers in Low- and Middle-income countries. The link between infection and cancer has become well established over the past 50 years, and infection-associated cancer contribute up to 16% of cancers in developed countries and 33% in less developed countries. Preventive vaccines have been developed for only two cancer-causing viruses, highlighting both the opportunity to prevent infection-associated cancers by vaccination and the gaps that remain before vaccines can be developed for other cancer-causing agents. These gaps are due to incomplete understanding of the basic biology, natural history, epidemiology of many of the pathogens that cause cancer, the mechanisms they exploit to cause cancer, and how to interrupt progression to cancer in human populations. Early diagnosis or identification of lesions at high risk of progression represent the current most critical research area of the field supported by recent advances in genomics and proteomics technologies.