Infectious Agents and Cancer最新文献

{"title":"GYPC methylation performance in detecting cervical (pre)cancer in high-risk HPV-positive women using liquid-based cervical scrapes.","authors":"Xing Fan, Zhengjiao Tong, Saiping Mao, Fang Yu, Rong Wang","doi":"10.1186/s13027-025-00699-3","DOIUrl":"https://doi.org/10.1186/s13027-025-00699-3","url":null,"abstract":"<p><strong>Background: </strong>Methylation of host genes is promising for the triage of women with high-risk human papillomavirus (HR-HPV) infections. This study aimed to validate the potential value of Glycophorin C (GYPC) methylation (GYPC^m) in the early detection of cervical cancer.</p><p><strong>Methods: </strong>We recruited HR-HPV-positive women at the hospital outpatient clinic between August and December 2023, using cytology triage. The remaining exfoliated cervical cells were subjected to GYPC^m testing. A total of 549 cases were finally included for analysis: 156 cervicitis, 303 cervical intraepithelial neoplasia (CIN)1, 49 CIN2, 37 CIN3, and 4 CC.</p><p><strong>Results: </strong>The difference of GYPC^m ΔCp values between CIN1 and CIN2 was statistically significant in the pathology results of 549 participants (P < 0.001). The optimal threshold value of GYPC^m for detecting CIN2 + was 6.35. The GYPC^m adjusted ORs for CIN2 + and CIN3 + were 31.23 (95%CI: 16.53-58.99) and 34.68 (95%CI: 11.90-101.11), respectively. In all individuals, the sensitivity of GYPC^m for CIN2 + and CIN3 + was consistent with that of cytology (CIN2 + 83.3%; CIN3 + 90.2%), with higher specificity than that of cytology (CIN2 + 85.8 vs. 29.4%; CIN3 + 79.7 vs. 28.7%, all P < 0.001). The sensitivity and negative predictive value (NPV) of HPV16/18 combined GYPC^m for CIN3 + were not statistically different from those of HPV combined with cytology (sensitivity 95.1 vs. 97.6%, P = 0.317; NPV 99.4 vs. 99.0%, P = 0.613). The net reclassification improvement for HPV16/18 combined GYPC^m and its combined cytology to classify CIN2 + vs. < CIN2 was 48.8% (95%CI: 39.7-57.9%, P < 0.001), with a relative colposcopy referral rate of 50.1% (95%CI: 45.5-54.7%).</p><p><strong>Conclusion: </strong>GYPC^m has a higher CIN2 + OR and higher specificity and positive predictive value than cytology in HR-HPV-positive women and is a potential molecular biomarker for triaging HR-HPV (+) to detect cervical (pre)cancer.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"69"},"PeriodicalIF":2.8,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and genotype distribution of HPV infection among women in Chengdu from 2019 to 2024: a retrospective single-center study.","authors":"Xiaolin Zhou, Jian Ma, Liping He, Hepei Li","doi":"10.1186/s13027-025-00698-4","DOIUrl":"10.1186/s13027-025-00698-4","url":null,"abstract":"<p><strong>Objective: </strong>Human papillomavirus (HPV) infection is closely associated with the occurrence and development of cervical cancer. This study comprehensively investigates HPV infection and subtype distribution among women in Chengdu from 2019 to 2024, aiming to provide scientific evidence for screening, prevention, and optimization of HPV vaccination strategies against cervical cancer and related diseases.</p><p><strong>Methods: </strong>Cervical exfoliated cell specimens from 65,130 female patients attended Sichuan Jinxin Xinan Women & Children Hospital from 2019 to 2024 were collected and detected 26 HPV gene subtypes using gene chip technology.</p><p><strong>Results: </strong>Among the 65,130 women included in the study, 13,463 were HPV positive, with an overall detection rate of 20.67%. The single infection rate was 14.80%, and the multiple infection rate was 5.88%; the infection rates for pure HR-HPV, pure LR-HPV, and mixed infections were 13.86%, 3.90%, and 2.92%, respectively. The HPV detection rate was highest in those aged ≤ 20 years (46.01%) and among those aged > 60 years (35.37%), showing a bimodal distribution across ages. The top five HR-HPV subtypes detected were HPV52, 58, 16, 51, and 39, with infection rates of 3.71%, 2.81%, 2.56%, 1.83%, and 1.64%, respectively. The top three LR-HPV subtypes were HPV54, 42, and 40, with detection rates of 1.85%, 0.99%, and 0.93%, respectively. From 2019 to 2024, HPV detection showed a U-shaped trend, with a significant decrease in HPV16 detection rate and an increase in HPV42. Among other subtypes co-infected with the top five HR-HPV subtypes, HPV52 and HPV58 accounted for the highest proportion. After 2023, co-infections with LR-HPV increased.</p><p><strong>Conclusion: </strong>During 2019-2024, the HPV infection rate among women in Chengdu was high with an increase in detection rates after 2023. The co-infection patterns of HR-HPV are complex. Infection rates are highest among women aged ≤ 20 years and > 60 years. Priority should be given to young women for vaccination. HPV screening should be strengthened for women across different age groups. Developing vaccines targeting locally prevalent HPV subtypes is crucial for reducing infection rates and preventing cervical cancer and other HPV-related diseases.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"68"},"PeriodicalIF":2.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiologists and treatment response assessment: conventional and functional radiological criteria for predicting therapeutic response in virus-related and non-virus-related cancers.","authors":"Vincenza Granata, Roberta Fusco, Davide Pupo, Alessandra Alfieri, Ferdinando Caranci, Maria Giovanna Riga, Mariadora De Feo, Luisa Sgariglia, Gianpaolo Carrafiello, Michele A Karaboue, Antonella Petrillo, Francesco Izzo","doi":"10.1186/s13027-025-00695-7","DOIUrl":"10.1186/s13027-025-00695-7","url":null,"abstract":"<p><p>Precision medicine, which emphasizes individualized patient care rather than targeting a specific type of neoplasm, is becoming increasingly prevalent in oncology. Furthermore, the ability to combine multiple treatment modalities either concurrently or at different stages of the disease is enhancing both the safety and effectiveness of therapies. This, in turn, leads to improved patient outcomes, not only in terms of overall survival (OS) but also in physical well-being. Nevertheless, oncologic therapies can induce both local and systemic changes, as well as complications that are closely related to the type of treatment administered. Accurate interpretation of post-treatment imaging is therefore essential for timely and appropriate patient management.In this context, it is evident that radiologists specializing in oncology must be well-versed not only in the effects of various therapies but also in the different assessment criteria used to evaluate treatment efficacy accurately.The purpose of this article is to provide an overview of the various response evaluation criteria, beginning with conventional, size-based methods and progressing to functional approaches that rely on metabolic activity and Magnetic Resonance Imaging (MRI), while highlighting the respective advantages and limitations of each.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"67"},"PeriodicalIF":2.8,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486614/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell analysis reveals the regulatory role of ECT2 in HPV-driven cervical carcinogenesis process.","authors":"Shuyi Huang, Xiaoning Wei, Yong Wang, Yudi Tan, Shasha Yang, Jinkong Wei, Yuying Wei, Yuxuan Jin, Linqing Li, Junying Chen","doi":"10.1186/s13027-025-00696-6","DOIUrl":"10.1186/s13027-025-00696-6","url":null,"abstract":"","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"66"},"PeriodicalIF":2.8,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotyping HPV 16 and 18 in oral and oropharyngeal cancer: uncovering HIV as a key risk factor in a Ugandan cohort.","authors":"Annah Margret Biira, Christopher Kintu, Eriab Nkamba, Adriane Kamulegeya, Muwazi Louis, Michael Odida","doi":"10.1186/s13027-025-00691-x","DOIUrl":"10.1186/s13027-025-00691-x","url":null,"abstract":"<p><strong>Background: </strong>Human Papillomavirus has been associated with oral squamous cell carcinoma (OSCC) and oropharyngeal squamous cell carcinoma (OPSCC), involving several risk factors in different parts of the world. This study aimed to determine the prevalence of high-risk HPV 16 and HPV 18, and factors associated with this prevalence, among patients with oral and oropharyngeal squamous cell carcinoma who attended Mulago Hospital, Uganda, from 2010 to 2015.</p><p><strong>Methods: </strong>This was a retrospective study in which 174 tissue blocks from individual patients, with confirmed oral and oropharyngeal squamous cell carcinoma, were retrieved from the archives. The corresponding medical charts of the patients were reviewed for demographic and clinical data. HE stained sections of the tissue blocks were reviewed for reconfirmation of OSCC& OPSCC. The samples were genotyped for HPV 16, 18 using Multiplex PCR techniques. The data was analysed using R.</p><p><strong>Results: </strong>All 174 samples were confirmed positive for OSCC & OPSCC. HPV DNA was positive in 128 individuals (74%), 46 tested negative. HPV 16 alone occurred in 55 (32%) subjects and HPV 18 alone occurred in 37 (21%). Double infection was present in 36 individuals (21%). HIV was the only risk factor significantly associated with HPV 16 on OSCC& OPSCC (p = 0.018).</p><p><strong>Conclusions: </strong>Overall, HPV 16 and 18 are key etiological factors in oral and oropharyngeal squamous cell carcinoma pathogenesis in the Ugandan population, with a high prevalence. The results suggest that HIV positive individuals are at a higher risk of acquiring HPV 16 associated OPSCC. Alcohol consumption and cigarette smoking are not factors associated with HPV associated OPSCC in this study population.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"65"},"PeriodicalIF":2.8,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12465536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metronomic chemotherapy in locoregionally advanced nasopharyngeal carcinoma with residual EBV-DNA after induction chemotherapy.","authors":"Lin-Feng Guo, Liu-Yun Gong, Qin Lin, San-Gang Wu","doi":"10.1186/s13027-025-00694-8","DOIUrl":"10.1186/s13027-025-00694-8","url":null,"abstract":"<p><strong>Purpose: </strong>To analyze outcomes and evaluate the survival benefits of metronomic chemotherapy (MC) with S-1 in locoregionally advanced nasopharyngeal carcinoma (LANPC) patients with residual EBV-DNA after induction chemotherapy (IC).</p><p><strong>Methods: </strong>We retrospectively included patients diagnosed with LANPC between October 2015 and August 2021. All patients were treated with IC and had residual EBV-DNA after IC. Chi-square test, Kaplan-Meier methods, and Cox proportional hazards model were used for statistical analyses.</p><p><strong>Results: </strong>A total of 103 patients were identified, including 20 (19.4%) who received MC using S-1 for one year. Among these patients, 40 experienced disease progression, including 12 with locoregional recurrence (30.0%), 24 with distant metastasis (60.0%), and 4 with both locoregional recurrence and distant metastasis (10.0%). The 5-year locoregional recurrence-free survival, distant metastasis-free survival (DMFS), progression-free survival (PFS), and overall survival (OS) rates were 81.3%, 70.7%, 52.8%, and 72.1%, respectively. Multivariate prognostic analysis showed that post-IC residual EBV-DNA levels were independent prognostic factors for DMFS and PFS. Patients with EBV-DNA levels > 90 copies/mL had worse DMFS (hazard ratio [HR] 4.978, 95% confidence interval [CI] 1.413-17.535, P = 0.012) and PFS (HR 3.679, 95% CI 1.592-8.499, P = 0.002). Additionally, receiving MC was an independent prognostic factor for PFS and OS. Patients who received MC had better PFS (HR 0.310, 95% CI 0.120-0.802, P = 0.016) and OS (HR 0.100, 95% CI 0.013-0.745, P = 0.025).</p><p><strong>Conclusions: </strong>Our study highlights the poorer survival outcomes observed in LANPC patients with residual EBV-DNA levels following IC, as well as the potential survival advantages of MC in this subgroup.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"64"},"PeriodicalIF":2.8,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12461949/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic and transcriptional insights reveal hepatitis C virus as a driver of kidney cancer.","authors":"Qiankun Wang, Yi Wang, Tongxin Yin, Yuting Feng, Huihao Ren, Xiao Xiao, Jiaoyuan Li, Liming Cheng","doi":"10.1186/s13027-025-00693-9","DOIUrl":"10.1186/s13027-025-00693-9","url":null,"abstract":"<p><strong>Objectives: </strong>Observational studies suggest a potential link between Hepatitis C virus (HCV) infection and extrahepatic cancers, but the causal relationship remains unclear.</p><p><strong>Methods: </strong>We applied a two-sample Mendelian randomization (MR) approach to evaluate the causal relationships between HCV infection and various extrahepatic cancers. A two-step MR was used to identify potential mediators, followed by colocalization analysis to identify HCV-associated susceptibility genes (HSGs). A pan-cancer analysis using TCGA data was conducted, and a prognostic model based on HSGs was developed using least absolute shrinkage and selection operator (LASSO) regression and Cox models. Genetic risk score (GRS) analysis from the UK Biobank validated our findings.</p><p><strong>Results: </strong>We identified a causal link between genetic susceptibility to HCV infection and kidney cancer, both in univariable and multivariable MR analyses. The two-step MR identified five mediators in the causal pathway. IRF5 was highlighted as a key HSG in both the colocalization and pan-cancer analyses. Our prognostic model incorporating three HSGs predicted overall survival (OS) in kidney cancer patients. GRS analysis confirmed the association.</p><p><strong>Conclusions: </strong>The present study provides evidence supporting a causal link between HCV infection and the development of kidney cancer.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"63"},"PeriodicalIF":2.8,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12406469/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and genotype distribution of human papillomavirus (HPV) infections among West African populations: a systematic review and meta-analysis.","authors":"Larwanou Harouna Magagi, Adamou Lagare, Arnol Bowo-Ngandji, Fatimata Hassane, Abdoulaye Zeidou Maiga, Moussa Issa, Alkassoum Ibrahim, Adehossi Eric, Alzouma Maiyaki Zoubeirou","doi":"10.1186/s13027-025-00659-x","DOIUrl":"10.1186/s13027-025-00659-x","url":null,"abstract":"<p><strong>Background: </strong>Human papillomavirus (HPV) is one of the most common sexually transmitted infections worldwide. Several studies have examined HPV prevalence in West Africa, but comprehensive regional estimates remain unavailable. This study estimates the overall prevalence and genotype distribution of HPV in West African populations.</p><p><strong>Methods: </strong>We conducted a systematic search in Medline, Embase, Global Health, Web of Science, and Africa Index Medicus for articles published up to March 5, 2024. We estimated pooled HPV prevalence using a random-effects model. The Hoy et al. tool assessed the risk of bias, while the I² test measured between-study heterogeneity. Egger's regression test evaluated publication bias. The study was registered with PROSPERO (CRD42023463042).</p><p><strong>Results: </strong>The analysis included 145 studies from 12 West African countries, comprising 71,324 participants. The pooled prevalence of HPV was 42.3% (95% CI: 38.5-46.1%), with significant heterogeneity (I² = 99.0%; P < 0.001). HPV prevalence varied across population subgroups, with a significantly higher pooled prevalence of 81.4% (95% CI: 68.2-91.8%) among women with cervical cancer. Egger's test did not indicate significant publication bias (P > 0.05). Among high-risk HPV genotypes, HPV-16 (19.9%, 95% CI: 15.9-24.1%) and HPV-82 (18.9%, 95% CI: 7.5-33.7%) were the most prevalent. Among low-risk genotypes, HPV-81 was the most common, with a prevalence of 12.9% (95% CI: 7.1-20.0%).</p><p><strong>Conclusion: </strong>Human papillomavirus prevalence is high in West Africa, with diverse genotypes, including several with oncogenic potential. Targeted health interventions, including vaccination programs and enhanced screening efforts, are recommended to reduce HPV burden in this region.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"62"},"PeriodicalIF":2.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12403902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Helicobacter pylori, peptic ulcer disease, and colorectal cancer: a prospective study with genome-wide interaction analysis and Mendelian randomization.","authors":"Ziqi Wan, Jiarui Mi, Xiaoyin Bai, Dong Wu, Sunny Hei Wong","doi":"10.1186/s13027-025-00686-8","DOIUrl":"10.1186/s13027-025-00686-8","url":null,"abstract":"<p><strong>Introduction: </strong>Helicobacter pylori (HP) is a well-established gastric carcinogen. But it shows inconsistent association with colorectal cancer (CRC) across diverse study populations.</p><p><strong>Methods: </strong>We investigated participants from the UK biobank. HP-seropositive cases and peptic ulcer diseases (PUDs) were identified. The primary outcome was CRC. We estimated the cumulative incidence using a competing risk model measured by the hazard ratios (HRs) with 95% confidence intervals (CIs) with adjustment. We did a genome-wide interaction analysis to identify genetic variants that modify HP-serology-CRC associations with exploratory gene-based and gene-set analysis. We further did a bidirectional two-sample Mendelian randomization (MR) to investigate the causal relation of gene-proxied PUD on CRC of the European ancestry and of the East Asian ancestry.</p><p><strong>Results: </strong>We included 492,490 participants with a median follow-up of 14.7 years. HP sero-positivity did not significantly increase the incidence of CRC (adjusted HR = 0.76, 95% CI 0.53 - 1.10, p = 0.15). No SNP was identified to be significantly interacted with HP serology to modify CRC risk. The risk of CRC for PUD cases was not significantly different from non-PUD cases after adjusting (adjusted HR = 0.88, 95% CI 0.76 - 1.02, p = 0.09). The gene-proxied PUD causally increased the incidence of colon cancer of the East Asian ancestry (OR = 1.47, 95% CI = 1.00 - 2.15, p = 0.047), not of the European ancestry (OR = 1.03, 95% CI = 0.79 - 1.34, p = 0.82).</p><p><strong>Conclusions: </strong>Neither seropositivity for HP nor PUD showed a robust increase on the risk of CRC in a 15-year follow-up. The causal relation of PUD on CRC was significant of East Asian ancestry, not of European ancestry.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"61"},"PeriodicalIF":2.8,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12398068/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porphyromonas gingivalis and Fusobacterium nucleatum synergistically strengthen the effect of promoting oral squamous cell carcinoma progression.","authors":"Xiao Song, Jingfei Wang, Zhen Gu, Xinyi Qiu, Meng Yuan, Huiji Ke, Runzhi Deng","doi":"10.1186/s13027-025-00689-5","DOIUrl":"10.1186/s13027-025-00689-5","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the mechanism of action of Porphyromonas gingivalis (P. gingivalis) and Fusobacterium nucleatum (F. nucleatum) individually and synergistically on Cal-27 cells through transcriptome analyses to evaluate the mechanism evidence of periodontal pathogen involvement in oral squamous cell carcinoma.</p><p><strong>Methods: </strong>Cal-27 cells were treated with P. gingivalis and F. nucleatum individually or in combination. Cell proliferation was assessed via CCK-8 assay and EdU staining, while migration was evaluated using scratch assays. Transcriptomic sequencing analyzed molecular mechanisms underlying single and co-infections.</p><p><strong>Results: </strong>Synergistic treatment with P. gingivalis and F. nucleatum significantly enhanced Cal-27 cell proliferation and migration compared to either pathogen alone. Transcriptomics revealed that co-infection accelerated tumor cell cycle progression and amplified pro-inflammatory pathways, indicating stronger pro-tumorigenic effects.</p><p><strong>Conclusion: </strong>This study clarifies the cooperative tumor-promoting role of multiple bacterial species, providing potential therapeutic targets for oral squamous cell carcinoma in bacterial infection contexts and highlighting the importance of controlling oral microbiota.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"20 1","pages":"60"},"PeriodicalIF":2.8,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12398151/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144952771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}