Infectious Agents and Cancer最新文献

{"title":"Correction: Role of gut microbiota in cancer modulation: molecular mechanisms and emerging therapeutic strategies.","authors":"Subham Preetam, Jutishna Bora, Sthiti Porna Dutta, Nayan Talukdar, Sarvesh Rustagi, Shailendra Thapliyal, Sumira Malik, Neeraj Choudhary, Dinesh Kumar, Suresh Babu Kondaveeti","doi":"10.1186/s13027-026-00807-x","DOIUrl":"10.1186/s13027-026-00807-x","url":null,"abstract":"","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"21 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13673624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149206909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sampling constraints, sparse-cell estimates, and cross-sectional inference: considerations on hr-HPV prevalence and associated factors among HIV-positive women in Mekelle, Ethiopia.","authors":"Shweta Thusoo","doi":"10.1186/s13027-026-00801-3","DOIUrl":"10.1186/s13027-026-00801-3","url":null,"abstract":"<p><p>Shfare et al. report a high-risk human papillomavirus (hr-HPV) prevalence of 39.7% among HIV-positive women attending public health facilities in Mekelle, Ethiopia, and identify several clinical and behavioural predictors using multivariable logistic regression. The study addresses an important evidence gap in a high-burden setting and employs rigorous molecular detection methods. Nevertheless, three methodological considerations affect the interpretation of the findings. First, facility-based recruitment from selected antiretroviral therapy (ART) clinics may limit the generalizability of the reported prevalence estimate and observed associations to the broader population of women living with HIV. Second, several adjusted odds ratios are accompanied by wide confidence intervals, indicating limited precision and warranting cautious interpretation. Finally, the cross-sectional design precludes causal inference regarding the association between oral contraceptive use and hr-HPV infection because residual confounding and reverse causation cannot be excluded. Collectively, these issues suggest that the findings should be interpreted cautiously and highlight the need for prospective population-based studies to validate the reported risk factors before they inform screening or prevention strategies.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"21 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13617853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149074472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HPV knowledge, awareness, and vaccine beliefs among women attending gynecological care in Türkiye: implications for cervical cancer prevention.","authors":"Figen Efe Camili, Sevde Aksu","doi":"10.1186/s13027-026-00803-1","DOIUrl":"https://doi.org/10.1186/s13027-026-00803-1","url":null,"abstract":"<p><strong>Background: </strong>Despite the availability of effective HPV vaccines, vaccination rates remain low in many countries, including Türkiye. Cervical cancer prevention continues to rely largely on opportunistic screening. Although HPV knowledge, awareness, and vaccine beliefs are recognized as important determinants of vaccination behavior, how these factors interact among women attending gynecological care has not been fully elucidated.</p><p><strong>Methods: </strong>This cross-sectional study included 300 women aged 18-49 years attending the obstetrics and gynecology outpatient clinic of a university hospital in the Marmara region of Türkiye for gynecological examination. Data were collected using a sociodemographic questionnaire, the HPV Knowledge Scale (HPV-KS), the Human Papilloma Virus Awareness and Concern Scale (HPV-ACS), and the Carolina HPV Vaccination Attitudes and Beliefs Scale (CHPV-VABS). Group comparisons, correlation analyses, and multivariate analysis of variance were performed to examine associations between participant characteristics and HPV knowledge, awareness and concern, and vaccine beliefs.</p><p><strong>Results: </strong>Participants showed limited HPV knowledge (11.01 ± 8.77 out of 33), moderate HPV-related awareness and concern (43.57 ± 9.95), and moderate HPV vaccine attitudes and beliefs (37.18 ± 7.42). HPV vaccination uptake was low, with only 12.3% of participants reporting prior vaccination. Higher educational attainment was associated with greater HPV knowledge scores (p < .001), and vaccinated participants had significantly higher knowledge scores than unvaccinated participants (p < .001). Education level was also significantly associated with the combined outcome of HPV knowledge, awareness and concern, and vaccine beliefs (F(3, 293) = 2.76, p=.044, η²p = .05). Correlation analyses showed strong positive associations between HPV knowledge and HPV-related awareness and concern (r = .60, 95% CI [0.52, 0.67], p < .001) and between HPV-related awareness and concern and vaccine beliefs (r = .61, 95% CI [0.53, 0.68], p < .001). In contrast, HPV knowledge showed a weak inverse correlation with vaccine beliefs (r = - .16, 95% CI [- 0.27, - 0.05], p = .005).</p><p><strong>Conclusion: </strong>Women attending gynecological examinations demonstrated limited HPV knowledge and very low HPV vaccination uptake despite moderate levels of HPV-related awareness and concern. These findings suggest that awareness alone may be insufficient to promote vaccination and highlight the importance of improving HPV knowledge through targeted educational interventions. As higher educational attainment was associated with greater HPV knowledge, integrating structured counseling and evidence-based communication into routine gynecological care may help address knowledge gaps and strengthen cervical cancer prevention efforts in Türkiye.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"21 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13629121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149109045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatitis B virus pregenomic RNA is associated with malignant phenotypes and postoperative survival in HBV-related intrahepatic cholangiocarcinoma.","authors":"Qiyu Tian, Wen Li, Pengpeng Li, Yuanyuan Wang, Judian Yu, Jian Yu, Gang Huang","doi":"10.1186/s13027-026-00792-1","DOIUrl":"10.1186/s13027-026-00792-1","url":null,"abstract":"<p><strong>Background: </strong>Chronic hepatitis B virus (HBV) infection contributes to infection-associated liver carcinogenesis. However, the biological and clinical relevance of HBV pregenomic RNA (pgRNA), a marker of residual viral transcriptional activity, in HBV-related intrahepatic cholangiocarcinoma (iCCA) remains insufficiently defined.</p><p><strong>Methods: </strong>This single-center retrospective exploratory translational study included 45 patients with HBV-related iCCA who underwent curative resection after long-term nucleos(t)ide analogue therapy and had suppressed preoperative serum HBV DNA. Tissue pgRNA expression was measured by quantitative reverse-transcription polymerase chain reaction (qRT-PCR), serum pgRNA was quantified by simultaneous amplification and testing (SAT), and spatial localization was assessed by immunofluorescence-fluorescence in situ hybridization (IF-FISH). Stable pgRNA-overexpressing HuCCT1 and RBE models were used to evaluate cell viability, migration, invasion, colony formation and stemness-related protein expression. Associations between serum pgRNA status and postoperative outcomes were examined using Kaplan-Meier, Cox regression and restricted mean survival time analyses.</p><p><strong>Results: </strong>Tumor tissues showed lower overall pgRNA expression than paired adjacent non-tumor liver tissues, whereas pgRNA signals were detectable in a subset of CK19-positive tumor epithelial cells. In HuCCT1 and RBE cells, pgRNA overexpression was associated with increased cell viability, migration, invasion, colony formation and upregulation of multiple stemness-related proteins. In the clinical analysis, the serum pgRNA HIGH group had poorer overall survival (adjusted HR = 5.22, 95% CI 1.82-14.95; P = 0.002) and recurrence-free survival (adjusted HR = 3.51, 95% CI 1.26-9.79; P = 0.017) than the BLQ group after adjustment for tumor size.</p><p><strong>Conclusions: </strong>HBV pgRNA may be linked to malignant and stemness-related phenotypes in HBV-related iCCA. High preoperative serum pgRNA was associated with poorer postoperative overall and recurrence-free survival in this exploratory cohort. These findings support validation of pgRNA as a candidate infection-related biomarker in larger prospective multicenter studies.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"21 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13573472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148968070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bladder cancer in Africa, 2010-2023: burden, geographic heterogeneity, demographic drivers, pandemic-era temporal changes, and projections to 2030.","authors":"Yongli Jin, Yan Jin, Yan Cui","doi":"10.1186/s13027-026-00782-3","DOIUrl":"https://doi.org/10.1186/s13027-026-00782-3","url":null,"abstract":"<p><strong>Purpose: </strong>To characterize the burden, temporal trends, geographic heterogeneity, demographic components, pandemic-era deviations, and projected age-standardized rates of bladder cancer across Africa.</p><p><strong>Methods: </strong>We conducted an ecological secondary analysis of modeled Global Burden of Disease Study 2023 estimates for the GBD aggregate labeled \"African Union\" and 54 African country locations from 2010 to 2023. Incidence, prevalence, deaths, and disability-adjusted life-years (DALYs) were assessed as counts and age-standardized rates (ASRs) per 100,000 population for both sexes combined. Trends were summarized using estimated annual percentage changes. Incidence trajectories were descriptively grouped using dynamic time warping, and changes in counts were partitioned into population-size, age-structure, and age-specific rate components using Das Gupta decomposition. Exploratory autoregressive integrated moving average models compared GBD-estimated rates in 2020-2023 with trajectories extrapolated from 2010 to 2019. Country-specific ASRs were projected through 2030 using log-linear models.</p><p><strong>Results: </strong>For the GBD aggregate labeled \"African Union,\" incident cases increased from 16,357 in 2010 to 31,503 in 2023, prevalent cases from 73,806 to 147,599, deaths from 9,261 to 16,467, and DALYs from 232,414 to 415,746. Corresponding ASRs increased from 3.5 to 4.4 for incidence, 13.9 to 18.3 for prevalence, 2.2 to 2.6 for deaths, and 48.1 to 56.1 for DALYs. The highest estimated incidence ASRs in 2023 occurred in Tunisia, Malawi, Libya, Egypt, and Mali, while several countries with low estimated baseline rates had rapid relative increases. Population-size change was the largest decomposition component, accounting for 39.8%-58.8% of the increase across outcomes, with additional contributions from age structure and age-specific rates. GBD-estimated incidence, mortality, and DALY rates during 2020-2023 were above the extrapolated point forecasts; however, this exploratory analysis did not establish a causal pandemic effect. The primary projection models yielded higher fitted 2030 ASRs in a majority of countries, although results varied across training windows.</p><p><strong>Conclusion: </strong>GBD 2023 estimates indicate an increasing but geographically heterogeneous bladder cancer burden across Africa. Population growth was the largest contributor to increasing counts, while age-structure and age-specific rate changes also contributed. Regionally tailored prevention, diagnostic and treatment capacity, and improved cancer surveillance are needed. Pandemic-era deviations and 2030 projections should be interpreted as model-dependent rather than causal or definitive forecasts.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":"21 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149223947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic interactions between Plasmodium falciparum and Epstein-Barr virus in endemic Burkitt Lymphoma pathogenesis: a narrative review (2010-2026).","authors":"Natalia A Msami","doi":"10.1186/s13027-026-00780-5","DOIUrl":"10.1186/s13027-026-00780-5","url":null,"abstract":"<p><p>Endemic Burkitt Lymphoma, characterized by an MYC translocation during B-cell activation, is an aggressive, fast-proliferating cancer that predominantly accounts for more than half of all childhood cancers in sub-Saharan Africa. Although a well-established epidemiologic association exists between Epstein-Barr virus infection and Plasmodium falciparum malaria, disentangling their mechanistic interactions in the pathogenesis of endemic Burkitt Lymphoma remains difficult. To address this challenge, a comprehensive narrative review of studies published between 2010 and 2026, was conducted. Evidence from mouse models, in vitro and ex vivo studies was synthesized to identify potential key effector cells, cytokine groups, and molecular pathways likely involved in fostering an immune environment that supports lymphomagenesis. The synthesized evidence informed the development of a speculative multi-hit model of endemic Burkitt Lymphoma, in which chronic Plasmodium falciparum exposure reconfigures the immune landscape through sustained inflammation, altered effector cell function, and impaired immune surveillance. Within this framework, these changes may subsequently facilitate Epstein-Barr virus persistence, intermittent B-cell reactivation, and cumulative genomic instability, ultimately increasing the likelihood of MYC translocations and the development of endemic Burkitt Lymphoma. The review also proposes incorporating emerging immune effector populations into this conceptual multi-hit model. Although several of the mechanistic relationships explored remain inferential, the framework identifies potential therapeutic and preventive immune targets that warrant further investigation in experimental and longitudinal human studies in high-burden regions.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13651022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148455723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HIV-associated malignancies in Tunisia: a 30-year retrospective cohort study from a North African perspective.","authors":"Sarra Yacoub, Manel Ben Selma, Souha Ghachem, Mariem Ben Ticha, Hela Knani, Mariem Garma, Taghrid Tlili, Safouene Frini, Badreddine Sriha, Wissem Hachfi","doi":"10.1186/s13027-026-00779-y","DOIUrl":"10.1186/s13027-026-00779-y","url":null,"abstract":"<p><strong>Background: </strong>Despite major advances in antiretroviral therapy (ART), HIV-associated malignancies remain a significant cause of morbidity and mortality in low- and middle-income countries. In Tunisia, data on AIDS-defining cancers (ADCs) and non-AIDS-defining cancers (NADCs) among people living with HIV (PLWH) remain scarce.</p><p><strong>Methods: </strong>We conducted a descriptive retrospective cohort study over a 30-year period (1994-2024) at Farhat Hached University Hospital, Sousse, Tunisia. All PLWH who developed at least one malignant neoplasm were included. Epidemiological, clinical, immunovirological, pathological, and outcome data were retrospectively collected and analyzed.</p><p><strong>Results: </strong>Among 655 PLWH followed during the study period, 25 patients with a median age of 36 years developed malignancies, representing 3.8% of all PLWH. 27 malignancies were identified with 2 patients developing 2 distinct malignancies: a Kaposi sarcoma (KS) and a Non-AIDS-defining cancer (NADC). AIDS-defining cancers (ADC) predominated (81.5%), mainly KS (44.4%) and non-Hodgkin lymphoma (33.3%), while NADCs accounted for 18.5%. Cancer and HIV diagnoses were concomitant in 56% of patients (n = 14) and 92% of malignancies occurred within the first year following HIV diagnosis. Most patients presented with advanced immunodeficiency, with median CD4 counts at tumor diagnosis of 73 cells/mm³ for ADCs and 57 cells/mm³ for NADCs. Median overall survival was 1.9 years for ADCs and 3.7 years for NADCs.</p><p><strong>Conclusions: </strong>In this North African cohort, HIV-associated malignancies were predominantly AIDS-defining cancer and affected young adults with advanced immunosuppression, reflecting late HIV diagnosis for PLWH developing malignancies. Beyond strengthening early HIV testing and timely ART initiation, targeted patient education focusing on high-risk behaviors, along with efforts to reduce HIV-related stigma, are essential components to improve cancer prevention, early diagnosis, and outcomes among PLWH in resource-limited settings.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13660005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148446045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diverse non-vaccine high-risk HPV genotypes in rural Kerala: implications for cervical cancer prevention in an underserved population.","authors":"Anusha M Sharma, Sachin David, Parasmal Suresh, Sivadas Swathi Krishna, Neethu Puthalon Kunnath, Beena Karimbuvayalil Vasudevan, Lalitha Biswas, Keechilat Pavithran","doi":"10.1186/s13027-026-00778-z","DOIUrl":"10.1186/s13027-026-00778-z","url":null,"abstract":"<p><strong>Background: </strong>The distribution of high-risk human papillomavirus (hrHPV) genotypes varies across populations and directly influences cervical cancer prevention strategies. Data from underserved rural populations remain limited.</p><p><strong>Methods: </strong>A community-based screening study was conducted among 358 women from rural and tribal regions of Kerala, India. High-risk HPV was detected using real-time PCR, and non-16/18 genotypes were identified through L1 gene sequencing and phylogenetic analysis.</p><p><strong>Results: </strong>The prevalence of hrHPV infection was 2.23% (8/358). Detected genotypes included HPV16, HPV31, HPV35, HPV45, HPV66, and HPV70. All detected infections were due to high-risk HPV genotypes, of which three were non-vaccine high-risk types. Phylogenetic analysis demonstrated close similarity with globally circulating strains.</p><p><strong>Conclusions: </strong>Despite low prevalence, the presence of diverse non-vaccine hrHPV genotypes highlights a potential gap between circulating strains and current vaccine coverage. These findings highlight the importance of continued region-specific HPV surveillance in underserved populations and provide baseline epidemiological data for future studies evaluating cervical cancer prevention strategies.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13632320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148411825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic, transition and variation of cervicovaginal microbiome and HPV infection and cervical dysplasia and cancer: a systematic review.","authors":"Gbenankpon Mathias Houvessou, Nerys Wendy Antonieta Alfane, Manuel Mahoche","doi":"10.1186/s13027-026-00777-0","DOIUrl":"10.1186/s13027-026-00777-0","url":null,"abstract":"<p><strong>Background: </strong>Cervical cancer is the fourth most common malignancy in women worldwide, with approximately 660,000 new cases and 350,000 deaths annually. The burden falls disproportionately on low- and middle-income countries. Although persistent infection with high-risk HPV (hrHPV) is the necessary cause, most infected women clear the virus spontaneously, implicating additional cofactors, including the cervicovaginal microbiome in determining oncogenic outcomes.</p><p><strong>Methods: </strong>PubMed was searched through September 10, 2024, to identify longitudinal studies assessing cervicovaginal microbiota in relation to HPV infection or cervical lesion outcomes at two or more time points. Methodological quality was evaluated using the Newcastle-Ottawa Scale (NOS). Given the substantial heterogeneity, a structured thematic synthesis was performed across three predefined domains: (a) baseline microbiome composition and clinical outcomes; (b) community state type (CST) dynamics and temporal stability; and (c) microbiome changes following treatment.</p><p><strong>Results: </strong>Twelve studies enrolling 1,663 women across 11 countries met inclusion criteria. NOS scores ranged from 4 to 9. Lactobacillus-dominated CSTs at baseline were consistently associated with HPV clearance and CIN regression, while Lactobacillus-depleted states showed higher transition rates and unfavourable outcomes. Prior L.iners (CST III) dominance was repeatedly linked to favourable outcomes, although evidence on this species remains conflicting. Cervicovaginal dysbiosis frequently preceded HPV persistence or lesion progression.</p><p><strong>Conclusion: </strong>Sustained Lactobacillus-dominated CST stability, rather than dominance by any single species, is the most consistent microbiome factor associated with favourable HPV and cervical lesion outcomes. Standardized longitudinal designs incorporating metagenomic sequencing, frequent sampling intervals, and rigorous confounder adjustment are needed to advance mechanistic understanding.</p><p><strong>Clinical trial registration number: </strong>Not applicable.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13628985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148404509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interoperability of two commercial kits in detecting high oncogenic human papilloma virus and genetic diversity in Cameroon: implications for cervical cancer screening strategies in resource-limited settings.","authors":"Michel Carlos Tommo Tchouaket, Jeremiah Efakika Gabisa, Ezechiel Ngoufack Jagni Semengue, Larissa Gaelle Moko Fotso, Valentine Marie Ferré, Bernadette Fokou Bomgning, Keriane Diane Kambou Kountchou, Alex Durand Nka, Nadine Nguendjoung Fainguem, Yagai Bouba, Désiré Takou, Rachel Kamgaing, Aude Christelle Ka'e, Collins Ambe Chenwi, Samuel Martin Sosso, Charlotte Charpentier, Carlo-Federico Perno, Vittorio Colizzi, Fon Wilfred Mbacham, Joseph Fokam, Alexis Ndjolo","doi":"10.1186/s13027-026-00775-2","DOIUrl":"10.1186/s13027-026-00775-2","url":null,"abstract":"<p><strong>Background: </strong>Cameroon lacks a national screening algorithm for Human papilloma virus (HPV) infection. In order to standardize HPV screening using molecular detection tools in Cameroon, this study aimed to evaluate the concordance of HPV detection via two multiplex systems and profile the epidemiology of High-risk oncogenic HPV (HR-HPV) circulating locally.</p><p><strong>Methods: </strong>A cross-sectional study was conducted on endocervical samples collected among women in Cameroon. HR-HPV detection and genotyping was done using Abbott and Sacace kits simultaneously. Discordant results were retested using Anyplex II HPV28 kit.</p><p><strong>Results: </strong>Of 364 participants enrolled (median-age: 42 [IQR:34-50] years), overall HR-HPV positivity was 21.4% (78/364) versus 15.4% (56/364) using Abbott and Sacace, respectively. Detection concordance between the test kits was strong (kappa-value = 0.8). Of 22 samples with discordant results, 86.36% (19/22) were further confirmed with Anyplex II HPV28; yielding an overall HR-HPV positivity prevalence of 20.6% (75/364), with 12 genotypes circulating. The most prevalent genotypes were HR-HPV_16 (18.8%), HR-HPV_18 (16.0%), HR-HPV_39 (16.0%), and HR-HPV_58 (13.0%). Discrepancy in the detection of different and/or same genotype was more prominent for HPV 18 (6/9). Though Sacace is suitable to further discriminate among genotypes, cost-analysis showed that Abbott was 50.6% cheaper in reagents per test.</p><p><strong>Conclusions: </strong>This study revealed a strong concordance between Abbott and Sacace kits for HR-HPV detection, demonstrating interoperability of these two multiplex systems available locally.</p>","PeriodicalId":13568,"journal":{"name":"Infectious Agents and Cancer","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13343902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148396710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}