Inflammopharmacology最新文献

{"title":"Inhibition of TRPM3 channels in the medial prefrontal cortex mitigates OCD symptoms following traumatic brain injury.","authors":"Gajendra N Pardeshi, Noor Ali, Kamini R Shirasath, Sameer N Goyal, Kartik T Nakhate, Sanjay N Awathale","doi":"10.1007/s10787-025-01763-5","DOIUrl":"https://doi.org/10.1007/s10787-025-01763-5","url":null,"abstract":"<p><p>Although tumor necrosis factor-alpha (TNF-α) plays an important role in the development of obsessive-compulsive disorder (OCD), the pathogenesis remains unclear. Since transient receptor potential melastatin 3 (TRPM3) channels are activated during inflammatory conditions, crosstalk with TNF-α in the progression of OCD has not been investigated yet. We hypothesize that mild traumatic brain injury (mTBI) stimulates TRPM3 channels, thereby enhancing the level of TNF-α in the medial prefrontal cortex (mPFC), a key brain region implicated in OCD pathogenesis. The closed-head weight-drop method was used for mTBI-induced OCD in mice, and neurological assessment was carried out using rotarod and beam-walk tests. Marble-burying test, open-field test, dark-light emergence test, and nest-building behavior test were performed to examine OCD-like symptoms. The mPFC was isolated, and the TNF-α level and TRPM3 immunoreactivity were estimated using ELISA and immunohistochemistry techniques. Additionally, Golgi-Cox staining and HPLC were performed to quantify dendritic arbor and serotonin content. To validate our hypothesis, mTBI mice were treated with a selective TRPM3 inhibitor naringenin (50 mg/kg) via intraperitoneal route, and all the above parameters were screened. Marble-burying and nest-building behaviors were increased in mTBI mice. However, exploratory behavior and time spend in the light chamber were significantly reduced. Moreover, mTBI increases TNF-α concentration and TRPM3 immunoreactivity, while decreasing dendritic arbor and serotonin content. Notably, naringenin treatment reversed these behavioral, biochemical, and molecular abnormalities. Naringenin may inhibit TRPM3-mediated TNF-α production and serotonin transmission, thereby suppressing OCD symptoms. Thus, we propose a novel therapeutic approach for treating OCD associated with traumatic brain injury.</p>","PeriodicalId":13551,"journal":{"name":"Inflammopharmacology","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PDE4 inhibitors in psoriasis therapy: current insights and future directions.","authors":"Amit Sharma, Niyati Pandoh, Keerti Dayal, Aditya Raj, Kirandeep Kaur, Navjot Kaur Sandhu, Vineet Kumar Rai, Shubham Thakur, Balak Das Kurmi","doi":"10.1007/s10787-025-01778-y","DOIUrl":"https://doi.org/10.1007/s10787-025-01778-y","url":null,"abstract":"<p><p>The chronic autoimmune disease known as psoriasis creates major life-quality problems for affected patients. The disease evolves through genetic and environmental triggers that trigger continuous keratinocyte multiplication with persistent inflammation. Research into psoriasis treatment now emphasizes phosphodiesterase-4 (PDE4) inhibitors because they manage cyclic adenosine monophosphate (cAMP) signaling to reduce inflammatory cytokine production. This review examines the PDE4 inhibitor potential for psoriasis treatment through investigation of their therapeutic functions alongside their mechanism of action and clinical response, safety data, and novel treatment approaches. The review gathered detailed information about clinical research and pharmaceutical operations of PDE4 inhibitors, specifically for apremilast, roflumilast, and crisaborole. This review also analyzes experimental PDE4 inhibitors alongside the advantages that appear when combining these drugs with biologics or phototherapy, together with methotrexate. The administration of PDE4 inhibitors creates higher intracellular cAMP levels that decrease TNF-α and IL-17 production as pro-inflammatory cytokines. The research on Apremilast, as the most prominent oral PDE4 inhibitor, shows that it both improves PASI scores and maintains good safety results. These medications treat psoriasis symptoms specifically through creams and gels, thus reducing the risk of whole-body side effects. The combined administration of PDE4 inhibitors and biologics leads to better therapeutic effects, which may lower both drug resistance rates and side effects. PDE4 inhibitors function as a valuable alternative therapeutic approach to both standard immunosuppressants and biologic medications in psoriasis treatment. New drug dosage methods connected to personalized treatment plans have the potential to raise patient care effectiveness. Researchers need to study ways to improve PDE4 inhibitor formulations, along with developing new combination therapy approaches to enhance sustained psoriasis disease treatment.</p>","PeriodicalId":13551,"journal":{"name":"Inflammopharmacology","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding the role of glucocorticoid-regulated kinase 1 in Alzheimer's disease: a promising path toward novel therapeutic strategies.","authors":"Yukti Mittal, Pankaj Kumar, Kajal Joshi, Khadga Raj Aran","doi":"10.1007/s10787-025-01777-z","DOIUrl":"https://doi.org/10.1007/s10787-025-01777-z","url":null,"abstract":"<p><p>Serum glucocorticoid-regulated kinase 1 (SGK1) is a ubiquitous serine and threonine kinase and has been implicated in many physiological processes including cell survival, proliferation, metabolism, and ion transport. The dysregulation of SGK1 has also been linked to various diseases including cardiometabolic diseases, cancer, and neurological disorders. Recent evidence indicates that SGK1 is influential in the key Alzheimer's disease (AD) pathologic mechanisms including memory and cognitive dysfunction and AD hallmarks such as amyloid beta (Aβ) plaques and neurofibrillary tangles. Overexpression of SGK1 affects the Aβ metabolism and affects the pathway and enzymes disserting Aβ. SGK1 also increases dendritic spine density through regulation of actin polymerization, which increases the ratio of synaptic contacts leading to possible enhancement of memory and cognitive function. The modulation of SGK1 dysfunction in AD pathology leads to tau hyperphosphorylation through glycogen synthase kinase-3 (GSK-3β), thereby promoting the formation of neurofibrillary tangles (NFTs). In addition, SGK1 enhances neuroinflammation through the activation of microglia as well as astrocytes into the release of pro-inflammatory cytokines and neuronal damage. Consequently, SGK1 has been implicated in pathological processes in neurodegeneration and further research is required to delineate its dual role. In this review, we focus on the role of SGK1 in neurodegenerative diseases, specifically in AD. In addition, it discusses the role of SGK1 signaling pathways and the possible SGK1 as a therapeutic target in memory formation and Aβ metabolism.</p>","PeriodicalId":13551,"journal":{"name":"Inflammopharmacology","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dapagliflozin targets the crosstalk between apoptosis, autophagy, and Hedgehog signaling pathways through AMPK activation in the adjuvant-induced arthritic rat model.","authors":"Aya A El-Demerdash, Samar F Darwish, Marwa O El-Derany, Ebtehal El-Demerdash","doi":"10.1007/s10787-025-01750-w","DOIUrl":"https://doi.org/10.1007/s10787-025-01750-w","url":null,"abstract":"<p><p>Rheumatoid arthritis is a long-term autoimmune disorder, causes joint capsule, cartilage, and bone damage. Dapagliflozin, a novel antidiabetic drug, demonstrated promising effects against different disorders. Herein, we aimed to detect the dose-dependent antiarthritic impact of dapagliflozin alone and in combination with methotrexate standard treatment. Complete Freund's adjuvant-induced arthritic rats were treated with three doses of dapagliflozin (1, 5, or 10 mg/kg/day, p.o.) for 3 weeks, in which 10 mg dose showed eminent anti-arthritic effects according to gait score, paw diameter, arthritic index (AI), morphological and histological results. To reveal dapagliflozin mechanism, locomotor, biochemical, and histological measures were assessed in dapagliflozin (10 mg/kg/day) and/or methotrexate (0.75 mg/kg/week, i.p.)-treated arthritic rats. Radiography and histology confirmed the prominent anti-arthritic effect of dapagliflozin via reduced RF, MMP-1, and MMP-3, and improved gait score, ankle diameter, and AI. Anti-inflammatory impact was confirmed by the downregulation of TNF-α, IL-1β, IL-6, and NF-κb p65 expression. Upregulation of autophagy was detected through; Beclin-1, ULK-1, and ATG-7, in dapagliflozin treated arthritic rats. Furtherly, dapagliflozin stimulated apoptotic activity, by boosting articular levels of CASP-3, CASP-9, cartilage gene expression of p53, and Bax/Bcl₂ ratio. Interestingly, dapagliflozin upregulates p-AMPK/t-AMPK articular activity. Additionally, dapagliflozin inhibited the Hedgehog signaling pathway, through the downregulation of cartilage Shh, ptch1, Smo, and Gli-1 expression. Dapagliflozin/methotrexate combination therapy exhibited greater anti-arthritic benefits compared to methotrexate alone. These data highlight dapagliflozin as an anti-rheumatic drug, either alone or with methotrexate.</p>","PeriodicalId":13551,"journal":{"name":"Inflammopharmacology","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the potential of Moringa oleifera seed extract-loaded microemulsion hydrogel in the DNCB-induced atopic dermatitis model.","authors":"Prakrati Garg, Aaliya Ali, Sewa Singh, Kanika Thakur, Kaisar Raza, Bigul Yogeshver Bhardwaj, Saurabh Kulshrestha, Poonam Negi","doi":"10.1007/s10787-025-01740-y","DOIUrl":"https://doi.org/10.1007/s10787-025-01740-y","url":null,"abstract":"<p><strong>Background: </strong>Moringa oleifera n-hexane (MO n-Hex) seed extract contains phytoconstituents such as behenic acid, quercetin, and kaempferol. These exhibit anti-inflammatory, antiallergic, and antioxidant properties and can alleviate atopic dermatitis (AD)-like symptoms.</p><p><strong>Purpose: </strong>This study aimed to develop and evaluate MO n-Hex seed extract-loaded microemulsion (ME) hydrogel to provide an effective, safe, non-steroidal, plant-based alternative to conventional therapies in the topical management of AD.</p><p><strong>Methods: </strong>Optimized o/w ME was developed by building pseudo-ternary phase diagrams. Ex vivo skin penetration was determined by employing CLSM analysis. Further, skin compatibility, histological analysis, and pharmacodynamics were carried out using a DNCB-induced AD model in BALB/c mice.</p><p><strong>Results: </strong>The best o/w ME demonstrated nearly spherical globules with size < 50 nm, zeta potential - 28.83 ± 0.492, and pH value 5.433 ± 0.047. The in vivo efficacy revealed significant improvements in AD-like symptoms, healed ear skin lesions, and lowered IgE levels and inflammatory cytokiness (IL-4, IL-5, and IFN-γ). Further, histological analysis confirmed the restoration of skin structure, supporting the formulation's potential in skin barrier repair.</p><p><strong>Conclusions: </strong>The study demonstrated that the MO n-Hex seed extract-loaded MEs were suitable for topical use with improved penetration to deeper layers of skin while showing safety and better skin compliance. The formulated MEs effectively modulated immune responses and restored skin structure and barrier functioning.</p>","PeriodicalId":13551,"journal":{"name":"Inflammopharmacology","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From receptor to response: dissecting the TLR4 pathway in diabetic neuropathy.","authors":"Monserrat Hernandez-Reyes, Thura Tun Oo","doi":"10.1007/s10787-025-01774-2","DOIUrl":"10.1007/s10787-025-01774-2","url":null,"abstract":"<p><p>Diabetic neuropathy (DNP) is a common complication of diabetes that has a significant impact on the patient's quality of life. The primary objectives of clinical treatment for DNP these days are symptomatic pain management and glycemic control. Since there is currently no cure for nerve damage, the only objective is to alleviate discomfort and slow its progression. Pre-clinical research over the last decade has increasingly linked toll-like receptor 4 (TLR4)-mediated neuroinflammation as a major contributor to DNP development. The role of TLR4-mediated neuroinflammation in the pathophysiology of DNP is covered in this review, along with different therapeutic approaches that target TLR4-mediated neuroinflammation in DNP in pre-clinical research. Despite promising pre-clinical results, translating these findings into clinical practice remains a challenge, which we also discuss how to address and overcome in this review.</p>","PeriodicalId":13551,"journal":{"name":"Inflammopharmacology","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review of the impact of natural products in preventing drug-induced ototoxicity.","authors":"Rania S Salah, Asmaa A Mahmoud, Riham A El-Shiekh, Ahmed M El-Dessouki, Abrar Gomaa Abd-Elfattah Hassan, Samar S Khalaf","doi":"10.1007/s10787-025-01766-2","DOIUrl":"https://doi.org/10.1007/s10787-025-01766-2","url":null,"abstract":"<p><p>Ototoxicity, the property of certain drugs to cause hearing loss, is a significant concern in medical treatments, particularly with the use of chemotherapeutic agents like cisplatin and aminoglycosides. These drugs can lead to permanent sensorineural hearing loss (SNHL), affecting a substantial proportion of patients. Existing strategies to alleviate these side effects are limited, prompting interest in natural products as potential protective agents. Natural products are being investigated for their ability to counteract these mechanisms through anti-inflammatory and antioxidant properties. The review seeks to highlight the potential of these natural products as complementary therapies to conventional ototoxic medications, emphasizing their protective roles, which are involved in cochlear cellular damage and programmed cell death. Further research is essential to establish standardized protocols for their use and to ensure their integration into clinical practice as effective therapeutic options.</p>","PeriodicalId":13551,"journal":{"name":"Inflammopharmacology","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amelioration of pain and adjuvant-induced arthritis by syringic acid via modulation of behavioral parameters and inflammatory mediators i.e. TNF-α, Interleukins, MCP-1, NF-kB and COX-2.","authors":"Noviara Saleem, Syed Atif Raza, Alamgeer, Muhammad Khalil-Ur-Rehman, Rukhsana Anwar, Abrar Ahmed, Hafiz Muhammad Irfan","doi":"10.1007/s10787-025-01736-8","DOIUrl":"https://doi.org/10.1007/s10787-025-01736-8","url":null,"abstract":"<p><p>Syringic acid with reported anti-inflammatory attribute was investigated in the present study to assess its anti-arthritic potential at doses of 25 mg/kg, 50 mg/kg and 100 mg/kg using adjuvant-induced arthritic rats. The rat's paw size (mm), arthritic index and behavioral parameters were observed at baseline and subsequently at seven days' interval until the completion of the study, following complete Freund's adjuvant (CFA) induction. The animals were anesthetized on 28th day and blood samples were obtained for the determination of numerous biochemical, hematological, pro-inflammatory, anti-inflammatory and oxidative biomarkers. Afterwards, the weight of lymphoid organs and radiographic, as well as histopathological examinations of the inflamed paw, were conducted. Furthermore, molecular docking was done to find out the interaction between syringic acid and Interleukins-1β, tumor necrosis factor-α, Interleukins-6 (IL-6), Interleukins-4 (IL-4) and Cyclooxygenase-2 (Cox-2). Results revealed that chronic syringic acid administration significantly reduced the paw size, arthritic index, and showed improvement in behavioral parameters with retrieval of altered hematological and biochemical parameters. Treatment with syringic acid also exhibited substantial recovery from oxidative stress markers and lymphoid organ weight was retrieved. Upon quantitative real-time polymerase chain reaction (qRT-PCR) examination, syringic acid significantly reduced the mRNA expression of tumor necrosis factor-α and all the other inflammatory cytokines while enhancing the mRNA expression of anti-inflammatory cytokines. Decreased serum concentration of PGE2 was noted with syringic acid as determined by enzyme-linked immunosorbent assay (ELISA). Histopathological analysis and radiographs further confirmed the findings. Molecular docking studies showed good interaction between syringic acid and IL-1β, tumor necrosis factor-α, IL-6, IL-4 and COX-2 when compared against standard.</p>","PeriodicalId":13551,"journal":{"name":"Inflammopharmacology","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive potential of Tripleurospermum inodorum with detailed insight into anti-inflammatory activity through in vitro, in vivo evaluations and network pharmacology.","authors":"Marija Ivanov, Aleksandra Popov Aleksandrov, Jelena Božunović, Maria Inês Dias, Ricardo C Calhelha, Jelena Kulaš, Lillian Barros, Isabel C F R Ferreira, Dejan Stojković","doi":"10.1007/s10787-025-01769-z","DOIUrl":"https://doi.org/10.1007/s10787-025-01769-z","url":null,"abstract":"<p><p>This study evaluated Tripleurospermum inodorum extract for cytotoxic, anti-inflammatory, antioxidant, antimicrobial, and antibiofilm properties, alongside its phenolic profile, predicted pharmacological interactions and in vivo anti-inflammatory properties. The methanolic extract of T. inodorum was rich in apigenin derivatives, including apigenin-O-pentoside (5.234 mg/g) and apigenin-O-acetyl hexoside (4.929 mg/g), as identified using LC-DAD-ESI/MSⁿ. The extract demonstrated potent anti-inflammatory activity (IC₅₀ = 8.4 µg/mL) by inhibiting nitric oxide production in a RAW 264.7 macrophage model, a key mechanism in controlling inflammatory responses. Its cytotoxicity against NCI-H460 lung carcinoma cells (GI₅₀ = 62.9 µg/mL) suggests potential for targeting inflammation-driven carcinogenesis. Antioxidant activity, ranging from 204.4 (FRAP) to 442.2 (ABTS) mmol of gallic acid equivalents per 100 mg dry weight, highlights its role in mitigating oxidative stress-a critical driver of chronic inflammation. The extract also displayed moderate antimicrobial activity (MIC: 3-12 mg/mL) and strong antibiofilm potential (> 70% inhibition in a crystal violet assay), which are essential for managing infection-associated inflammation. Network pharmacology revealed that dominant phenolic compound act as aldose reductase inhibitors, targeting inflammatory pathways linked to metabolic stress. In vivo assessment using a xylene-induced ear edema model revealed a dose-dependent, biphasic anti-inflammatory effect, with lower doses (125 and 250 mg/kg) exhibiting greater efficacy compared to the highest dose (500 mg/kg), suggesting a hormetic response that emphasizes the importance of optimal dosing. These findings indicate that the methanolic extract of T. inodorum possesses a broad spectrum of bioactivities relevant to inflammation control and supports its further development as a source of novel anti-inflammatory therapeutics.</p>","PeriodicalId":13551,"journal":{"name":"Inflammopharmacology","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-arthritic potential and mechanistic insights of methanol extract of Rhamnus prinoides Engl. in complete Freund's adjuvant-induced rats.","authors":"James Kimani Kamau, Mathew Piero Ngugi, Joseph N Ngeranwa","doi":"10.1007/s10787-025-01770-6","DOIUrl":"https://doi.org/10.1007/s10787-025-01770-6","url":null,"abstract":"<p><p>This study determined the phytochemical profile and in vivo anti-arthritic potential and mechanistic effects of MeOH extract of Rhamnus prinoides. The liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) were used in the phytochemical analysis of the extract. In the anti-arthritic assay, the rats were assigned into arthritic control, non-arthritic control, methotrexate control, and three extract-treated [100, 200, and 300 mg/kg body weight (bw)] groups. Complete Freund's adjuvant was used to induce polyarthritis. The treatments were orally administered from day 8 post-induction of polyarthritis. The experimental animals were euthanized and blood was drawn for hematological parameter analysis on day 29. The ankle joint tissue and liver were detached and utilized in gene expression using RT-qPCR and standard antioxidant assays, respectively. One-factorial ANOVA and Tukey's multiple comparisons were used to compute for statistical differences of the raw data. The LC-MS analysis identified phytochemicals of flavonoids, anthraquinones, lignans, and coumarins classes. Fatty acid methyl ester, benzofuran, and terpene were also detected using GC-MS. The extract significantly reduced ankle joint edema, reduced body weight loss and arthritis scores, improved elevated spleen and thymus indices, attenuated aberrant hematological parameters, lowered malonaldehyde levels, and enhanced enzymatic antioxidant activities in rats induced with polyarthritis (p < 0.05). The extract also significantly upregulated expression of I-κBα, IL-4, and IL-10 genes, as well as downregulated expression of STAT-3, NF-κB, RANKL, COX-2, TNF-α, and IL-6 genes in rats induced with polyarthritis (p < 0.05). The extract possesses phytochemicals with anti-arthritic potential and can be used as a potential lead in developing novel anti-arthritic agents.</p>","PeriodicalId":13551,"journal":{"name":"Inflammopharmacology","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}