Immunity, Inflammation and Disease最新文献

{"title":"Seroprevalence of SARS-CoV-2 Antibody Before and After Both Vaccination and Natural Infection in China","authors":"Yang Guang,&nbsp;Liu Lina,&nbsp;Liu Hui","doi":"10.1002/iid3.70184","DOIUrl":"https://doi.org/10.1002/iid3.70184","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study aims to analyze the effects of temporal SARS-CoV-2 antibodies in China before and after both vaccination and natural infection, thereby providing an empirical basis for evaluating the effectiveness of various prevention methods, including vaccination.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>IgG antibodies against SARS-CoV-2 were determined using chemiluminescence immunoassays, and antibody data was collected from published articles starting in early 2020 and from patients scheduled for surgery at the Hospital of Dalian Medical University between January 2022 and January 2024.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A SARS-CoV-2 infection epidemic in Wuhan in January 2020 led to a 3.2% seropositivity rate of SARS-CoV-2 antibodies (total antibodies). While the seropositivity rate for SARS-CoV-2 antibodies in mainland China reached 37.2% following the implementation of China's zero-COVID policy and the immunization rate was above 90% in January 2022. By the end of 2022, the Chinese government eased strict control measures, resulting in a SARS-CoV-2 antibody (IgG) positivity rate of 86.7% in January 2023. In January 2024, the positivity rate for SARS-CoV-2 antibodies in post-pandemic was recorded at 94.0%. Antibody levels in the early part of 2023 were considerably higher than those measured in January 2022 (68.66 vs. 10.21, <i>p</i> &lt; 0.05); that in early 2024 were not substantially higher than those in January 2023 (49.29 vs. 68.66, <i>p</i> &gt; 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The results of this study indicated that the immune barrier established by inactivated vaccines could be disrupted by the natural infection with SARS-CoV-2, resulting in a higher level of antibody production than vaccination. This effect can last for more than a year.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13289,"journal":{"name":"Immunity, Inflammation and Disease","volume":"13 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/iid3.70184","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Psychological and Burnout Effects of the COVID-19 Pandemic on Individuals","authors":"Bahar Ürün Ünal,&nbsp;Neslihan İyit,&nbsp;Yunus Akdoğan,&nbsp;Burcu Gök Erdoğan,&nbsp;Yüksel Duygu Altıparmak","doi":"10.1002/iid3.70181","DOIUrl":"10.1002/iid3.70181","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>COVID-19 infection has affected individuals mentally and socially in many areas. Restrictions, fear of infection, and anxiety about the future have created a great psychological burden on people. In this study, we aimed to investigate the significant negative effects of the COVID-19 pandemic on individuals' mental health and psychological functioning.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A 42-question questionnaire was applied to a total of 557 participants, including 350 people over the age of 18 who applied to the COVID-19 Vaccination Unit of Selçuk University Faculty of Medicine Hospital between December 2021 and May 2022 to be vaccinated and 207 people with a simultaneous online survey application. The psychological effects experienced by the participants due to the COVID-19 epidemic were questioned with psychological distress and burnout scales. Differences in the demographic characteristics of the participants were investigated according to the fear and anxiety sub-dimension.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>According to the psychological distress scale, gender, COVID-19 vaccination status, educational status and place of residence were found to be significant. According to the burnout scale, gender, marital status, presence of chronic disease, COVID-19 posttest status, occupation and income status were found significant.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Limitations</h3>\u0000 \u0000 <p>The anxiety, fear, and stress levels reported by participants may not be consistent with an objective assessment by mental health professionals. The majority of participants were public sector employees and students, so the results regarding job loss anxiety cannot be generalized. No information was collected on participants' past medical psychiatric disorders within the scope of the study.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>It is obvious that stress and psychiatric disorders are more common in individuals with high perception of infectiousness and lethality of the agent, especially during epidemic periods. We think that this study will be useful for planning interventions to alleviate mental health problems of individuals in future epidemics.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13289,"journal":{"name":"Immunity, Inflammation and Disease","volume":"13 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: lncRNA NEAT1/miR-495-3p Regulates Angiogenesis in Burn Sepsis Through the TGF-β1 and SMAD Signaling Pathways","authors":"","doi":"10.1002/iid3.70177","DOIUrl":"10.1002/iid3.70177","url":null,"abstract":"<p><b>RETRACTION</b>: Y. Meng, Z. Hao, H. Zhang, P. Bai, W. Guo, X. Tian and J. Xu, “lncRNA NEAT1/miR-495-3p Regulates Angiogenesis in Burn Sepsis Through the TGF-β1 and SMAD Signaling Pathways,” <i>Immunity, Inflammation and Disease</i> 11, no. 1 (2023): e758, https://doi.org/10.1002/iid3.758.</p><p>The above article, published online on January 16, 2023 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement the journal Editor-in-Chief, Marc Veldhoen; and John Wiley &amp; Sons Ltd. The retraction has been agreed due scientific flaws and inconsistencies found in the methodology and results of this article. While the authors were able to provide some supporting data, this was not sufficient and the irregularities remain. The editors have lost confidence in the results and conclusions presented in this study. The authors disagree with the retraction.</p>","PeriodicalId":13289,"journal":{"name":"Immunity, Inflammation and Disease","volume":"13 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case Report: Concurrent Babesiosis and GCA/PMR","authors":"Xiaolin Wang,&nbsp;Haohua Huang,&nbsp;Lichang Chen,&nbsp;Simin Guo,&nbsp;Qi-Ming Gong","doi":"10.1002/iid3.70182","DOIUrl":"https://doi.org/10.1002/iid3.70182","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Babesiosis is a tick-transmitted illness caused by intraerythrocytic protozoa of the genus babesia. The severity of babesiosis ranges from asymptomatic infection to fatal disease. Giant cell arteritis (GCA) and polymyalgia rheumatica (PMR) are common interrelated inflammatory disorders that almost occur in people aged over 50 years. This report presents the first case of concurrent babesiosis and GCA/PMR in an old person.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Case Presentation</h3>\u0000 \u0000 <p>A 63-year-old man was admitted to the hospital with a 1-month history of fevers, accompanied by headache, muscle pain and fatigue. Laboratory tests revealed hemolytic anemia, with elevated C-reactive protein, serum IL-6 and erythrocyte sedimentation rate. FDG positron-emission tomography-computed tomography (PET-CT) scan exhibited increased uptake in aortic wall, multiple medium-to-large arteries and soft tissues. A blood smear revealed Babesia microti intracellular ring forms. Babesia microti infection was further confirmed by polymerase chain reaction (PCR) test. This patient was diagnosed as concurrent babesiosis and GCA/PMR. He was treated with glucocorticoid and antimicrobial therapy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Concurrent babesiosis and GCA/PMR is rare. Further studies are needed to understand the mechanism of interaction between babesiosis and human immune system.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13289,"journal":{"name":"Immunity, Inflammation and Disease","volume":"13 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/iid3.70182","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Defining the Blood Cytokine Profile in Asthma to Understand Asthma Heterogeneity","authors":"Karina Bingham,&nbsp;Yousef Al Zahrani,&nbsp;Iain Stewart,&nbsp;Michael A. Portelli,&nbsp;Andrew Fogarty,&nbsp;Tricia M. McKeever,&nbsp;Ananga Singapuri,&nbsp;Liam G. Heaney,&nbsp;Adel H. Mansur,&nbsp;Rekha Chaudhuri,&nbsp;Neil C. Thomson,&nbsp;John W. Holloway,&nbsp;Peter H. Howarth,&nbsp;Ratko Djukanovic,&nbsp;John D. Blakey,&nbsp;Anoop Chauhan,&nbsp;Christopher E. Brightling,&nbsp;Zara E. K. Pogson,&nbsp;Ian P. Hall,&nbsp;Luisa Martinez-Pomares,&nbsp;Dominick Shaw,&nbsp;Ian Sayers","doi":"10.1002/iid3.70116","DOIUrl":"https://doi.org/10.1002/iid3.70116","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Asthma is a heterogeneous disease characterized by overlapping clinical and inflammatory features.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study aimed to provide insight into the systemic inflammatory profile in asthma, greater understanding of asthma endotypes and the contribution of genetic risk factors to both.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>4205 patients with asthma aged 16–60 were recruited from UK centers; serum cytokines were quantified from 708, including cytokines associated with Type 1, 2 and 17 inflammation. 3037 patients were genotyped for 25 single nucleotide polymorphisms associated with moderate-severe asthma.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Serum cytokines associated with Th2 inflammation showed high coordinated expression for example, IL-4/IL-5 (<i>R</i>² = 0.513). The upper quartile of the serum cytokine data identified 43.7% of patients had high levels for multiple Th2 cytokines. However, the groups defined by serum cytokine profile were not clinically different. Childhood-onset asthma was characterized by elevated total IgE, allergic rhinitis and dermatitis. Exacerbation prone patients had a higher BMI, smoking pack-years, asthma control questionnaire score and reduced lung function. Patients with blood eosinophils of &gt; 300 cells/µL had elevated total IgE and lower smoking pack-years. None of these groups had a differential serum cytokine profile. Asthma risk alleles for; rs61816764 (<i>FLG</i>) and rs9303277 (<i>IKFZ3</i>) were associated with childhood onset disease (<i>p</i> = 2.67 × 10⁻⁴ and 2.20 × 10⁻⁷; retrospectively). No genetic variant was associated with cytokine levels.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Systemic inflammation in asthma is complex. Patients had multiple overlapping inflammatory profiles suggesting several disease mechanisms. Genetic risk factors for moderate-severe asthma confirmed previous associations with childhood onset of asthma.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13289,"journal":{"name":"Immunity, Inflammation and Disease","volume":"13 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/iid3.70116","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lymphocyte Subpopulations in the Healthy Human Lacrimal Gland and Their Variations With Age and Sex, Systematic Review 1960–2023","authors":"Claudia M. Trujillo-Vargas,&nbsp;Luisa María Rendón-Macías,&nbsp;Ronald Yamil Paredes Guerrero,&nbsp;Cinta S. de Paiva,&nbsp;Jaiberth Antonio Cardona-Arias","doi":"10.1002/iid3.70167","DOIUrl":"https://doi.org/10.1002/iid3.70167","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Background&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Immunosenescence has been associated with an imbalance in the lacrimal functional unit and histopathological changes in exocrine glands, especially in women.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Objective&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;To define the main lymphocyte subpopulations in the human lacrimal gland and their variations with age and sex, according to scientific articles published between 1960 and 2023.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;A systematic review was performed on PubMed, ScienceDirect, and Google Scholar. The PRISMA 2020 guidelines were applied for the search and selection of studies.&lt;/p&gt;\u0000 \u0000 &lt;p&gt;The methodological quality was evaluated with the STROBE guidelines. A meta-analysis of three selected articles dichotomizing lymphocytic infiltrates according to age group was also performed.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Results&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;We selected 20 observational studies, including 774 healthy individuals (722 cadavers). The articles evaluated the lymphocyte infiltration with hematoxylin and eosin staining, immunohistochemistry and immunofluorescence. There was high variability in the criteria to define the apparently human lacrimal and to quantify the lymphocytic infiltration. There was an underrepresentation of individuals younger than 40 years (12.6%), and female sex (38.9%). Three articles reported an association of age and sex with lymphocytic infiltration in the healthy lacrimal gland, while two articles did not. Plasma cells were the most abundant lymphocyte subpopulation in the healthy lacrimal gland, including IgA-containing plasma cells. B cells were reported to be very scarce in the LG in two articles. In the meta-analysis of three selected articles, no statistical difference in lymphocytic infiltration was found between individuals younger and older than 60.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Conclusion&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;There is the need of further observational studies, better defining the study design, with similar representation across sex and ages to ascertain what are the changes of lymphocytic composition in the lacrimal gland related to age and sex. Further studies are also needed to assess the dynamics of lymphocytic populations in a more detailed manner using cutting-edge methodologies such as single-cell sequencing or transcriptomics.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Trial Registration&lt;/h3&gt;\u0000 \u0000 ","PeriodicalId":13289,"journal":{"name":"Immunity, Inflammation and Disease","volume":"13 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/iid3.70167","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinicopathological Characteristics and Outcomes of Lupus Nephritis Patients With Thrombocytopenia: A Single-Center Retrospective Study","authors":"Hui Diao,&nbsp;Yuting Fan,&nbsp;Di Kang,&nbsp;Zhiqing Chen,&nbsp;Yuewen Lu,&nbsp;Xiamin Huang,&nbsp;Xi Xia,&nbsp;Wei Chen","doi":"10.1002/iid3.70179","DOIUrl":"https://doi.org/10.1002/iid3.70179","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The objective of this study is to analyze and summarize the clinical characteristics and prognosis of lupus nephritis (LN) patients with thrombocytopenia and to improve the cognition of the disease.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>896 LN patients were enrolled in this study and their clinical and pathological data were collected and analyzed. The primary end point was mortality. The secondary end point was adverse renal outcomes, defined as doubling of the baseline serum creatinine or end-stage renal diseases. Cox regression model was used to analyze the risk factors of mortality or renal events in LN with and without thrombocytopenia.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Among 896 LN patients, 70 (7.8%) were diagnosed with thrombocytopenia. LN patients with thrombocytopenia had lower estimated glomerular filtration rate (eGFR) and higher systemic lupus erythematosus disease activity index (SLE-DAI), proportion of anemia, leukopenia, hypocomplementemia, and positive anti-cardiolipin antibodies, compared to those without thrombocytopenia. LN patients with thrombocytopenia had higher scores of activity index and more activity features (endocapillary hypercellularity, medullary loop necrosis) on kidney biopsy. There was no significant difference in patient survival and renal survival between LN patients with and without thrombocytopenia. Anemia was a risk factor for death in LN patients with thrombocytopenia and lower eGFR was a risk factor for adverse renal outcomes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>LN patients with thrombocytopenia showed higher disease activity, more anti-cardiolipin antibody positivity and a higher activity index in kidney biopsy, but the prognosis was similar compared with those without thrombocytopenia. Anemia was a risk factor for death in LN patients with thrombocytopenia.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13289,"journal":{"name":"Immunity, Inflammation and Disease","volume":"13 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/iid3.70179","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor Cell-Expressed Herpesvirus Entry Mediator Regulates Proliferation and Adaptive Immunity in Ovarian Cancer","authors":"Yun Lu,&nbsp;Yijun Zhang,&nbsp;Wenxuan Li,&nbsp;Haonan Jiang,&nbsp;Jiapo Wang,&nbsp;Xiaoqing Guo","doi":"10.1002/iid3.70175","DOIUrl":"https://doi.org/10.1002/iid3.70175","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Ovarian cancer (OvCa) is a prevalent gynecological malignancy with an increasing incidence and high mortality rate. Although the role of the herpesvirus entry mediator (HVEM), encoded by the <i>TNFRSF14</i> gene, is currently considered pivotal in various types of cancer, the regulation of tumor cell-expressed HVEM in OvCa remains inadequately understood.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Specimens were used to detect HVEM expression via quantitative RT-PCR and flow cytometry. The proliferation of the murine OvCa cell line ID8 was determined using the Cell Counting Kit-8, colony formation, and EdU staining assays. The immune constituents within the ascites fluid and spleen of tumor-bearing mice were analyzed by flow cytometry. Bioinformatics analysis was performed to explore cytokines, chemokines, and signaling pathways regulated by HVEM, and differential expression levels were confirmed via quantitative RT-PCR and western blot analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Herein, we identified a significant upregulation of HVEM in OvCa tissues compared with that in benign tissues and observed dominant expression of HVEM in CD45⁻EpCAM⁺ subsets in OvCa specimens. Tumor cell-expressed HVEM was found to promote OvCa cell proliferation by partly activating spliced X-box-binding protein 1 (XBP1s)-c-Myc signaling. In mouse models, knockdown of <i>Tnfrsf14</i> in ID8 cells alleviated OvCa progression and specifically affected the frequency and function of T cells in the ascites fluid and spleen. In addition, tumor cell-expressed HVEM altered chemokine expression (CXCL1/9/10/11 and CCL2/4/5) and STAT signal activation (STAT5 and STAT6) in ID8 cells.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study investigated the effects of HVEM on OvCa and validated its potential as a therapeutic marker for treating OvCa.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13289,"journal":{"name":"Immunity, Inflammation and Disease","volume":"13 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/iid3.70175","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferroptosis-Related Genes Are Effective Markers for Diagnostic Targets of Crohn's Disease","authors":"Pengfei Liu,&nbsp;Qing Liu,&nbsp;Ye Tian,&nbsp;Pengpeng Cai,&nbsp;Jianan Bai","doi":"10.1002/iid3.70170","DOIUrl":"https://doi.org/10.1002/iid3.70170","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Crohn's disease (CD) is a group of chronic transmural inflammation of gastrointestinal tract, which seriously harms the mental and physical health of adolescents. At present, there are still no specific markers that make the diagnosis of CD extremely difficult and poor prognosis. Iron deficiency is common in CD, yet the role of ferroptosis-related genes in CD has not been elucidated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The serum iron and ferritin levels were detected in 107 newly diagnosed CD patients and 107 healthy volunteers in our hospital. Bioinformatics analysis was used to analyze the chip sequencing data of CD in GEO database. Immunohistochemical analysis of paired inflammatory and noninflammatory intestinal tissues from CD patients was performed to confirm the differential protein expression pattern of the target genes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Patients with CD exhibited significantly reduced serum iron and ferritin levels compared to healthy controls. Transcriptomic analysis identified 40 upregulated and 31 downregulated ferroptosis-associated genes in CD patients versus controls. LASSO regression and SVM-RFE algorithms prioritized 13 hub genes (e.g., <i>CDKN2A</i>, <i>LCN2</i>, <i>STAT3</i>, <i>MT1G</i>), with a ROC curve demonstrating 100% specificity for combined biomarker analysis. Despite robust bioinformatic predictions, serum RNA levels of <i>CDKN2A</i>, <i>LIG3</i>, and <i>MTF1</i> showed no intergroup differences. Immuno-reactivity score validated protein expression consistency for <i>LCN2, PANX1, LPIN1</i>, <i>PML</i>, <i>STAT3</i>, <i>PARP9</i>, <i>RELA</i>, <i>NEDD4</i>, and <i>MT1G</i> but not <i>PPARD</i> or <i>LCN2</i>. Expression patterns of these genes correlated with M0 macrophage infiltration, resting mast cells, and neutrophil recruitment, suggesting immune-microenvironment interactions in CD progression.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Combined detection of ferroptosis-related genes is of great value in the diagnosis of CD.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13289,"journal":{"name":"Immunity, Inflammation and Disease","volume":"13 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/iid3.70170","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143622416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Lactate Dehydrogenase Levels Reflect the Lung Injury Extension in COVID-19 Patients at Hospital Admission","authors":"Maria Sofia Bertilacchi,&nbsp;Giulia Vannucci,&nbsp;Rebecca Piccarducci,&nbsp;Lorenzo Germelli,&nbsp;Chiara Giacomelli,&nbsp;Chiara Romei,&nbsp;Brian Bartholmai,&nbsp;Greta Barbieri,&nbsp;Claudia Martini,&nbsp;Michela Baccini","doi":"10.1002/iid3.70168","DOIUrl":"https://doi.org/10.1002/iid3.70168","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Several hematological and biochemical parameters have been related to the COVID-19 infection severity and outcomes. However, less is known about clinical indicators reflecting lung involvement of COVID-19 patients at hospital admission. Computed tomography (CT) represents an established imaging tool for the detection of lung injury, and the quantitative analysis software CALIPER has been used to assess lung involvement in COVID-19 patients. Herein, the relationship between the lung involvement expressed by CALIPER interstitial lung disease (ILD) percentage and a set of blood parameters related to tissue oxygenation and damage in COVID-19 patients at hospital admission was evaluated.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We performed a retrospective and a prospective study involving 321 and 75, respectively, COVID-19-positive patients recruited from Pisa University Hospital. The association between CALIPER ILD percentages and selected blood parameters was investigated by a regression tree approach, after multiple imputations of the dataset missing values.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>High serum lactate dehydrogenase (LDH) values appeared to be predictive of high CALIPER ILD percentages at hospital admission in both retrospective and prospective datasets, even if the predictive performance of the algorithm was not optimal.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>LDH levels could be evaluated as a tool for early identification of COVID-19 patients at risk of extensive lung injury, as well as in fast screening procedures before hospitalization.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13289,"journal":{"name":"Immunity, Inflammation and Disease","volume":"13 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/iid3.70168","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}