Indian Journal of Medical Microbiology最新文献_第4页

In vitro efficacy of levonadifloxacin against methicillin-resistant Staphylococcus aureus (MRSA) including hVISA isolates collected across India 左氧氟沙星对耐甲氧西林金黄色葡萄球菌（MRSA）的体外疗效，包括在印度收集的hVISA分离株。

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2025-01-01 DOI: 10.1016/j.ijmmb.2025.100788

Sneha Vinayan , Sujatha Sistla , Meerabai Manoharan , Kamini walia , Madhan Sugumar

{"title":"In vitro efficacy of levonadifloxacin against methicillin-resistant Staphylococcus aureus (MRSA) including hVISA isolates collected across India","authors":"Sneha Vinayan , Sujatha Sistla , Meerabai Manoharan , Kamini walia , Madhan Sugumar","doi":"10.1016/j.ijmmb.2025.100788","DOIUrl":"10.1016/j.ijmmb.2025.100788","url":null,"abstract":"<div><h3>Background</h3><div>Vancomycin has been the preferred treatment for MRSA infections. However, newer drugs are necessary due to the increasing prevalence of MRSA isolates that are less susceptible to vancomycin. Levonadifloxacin and its prodrug alalevonadifloxacin, novel quinolones with broad spectrum anti-MRSA activity. They are approved in India for the treatment of skin and soft-tissue infections, bacteraemia, and diabetic foot infections.</div></div><div><h3>Materials and methods</h3><div>A total of 456 MRSA (Methicillin-resistant <em>Staphylococcus aureus</em>) isolates, including 333 isolates from JIPMER and 123 isolates from other tertiary care centers collected between January to December 2022, underwent susceptibility testing to levonadifloxacin by disc diffusion method. The study isolates included 29 h-VISA. Fifty one blood isolates were also subjected to E-test and their results were compared to the gold standard, broth micro dilution (BMD).</div></div><div><h3>Result</h3><div>All 456 isolates were found to be susceptible to levonadifloxacin by disc diffusion. Levonadifloxacin demonstrated potent activity against MRSA strains isolated from blood (n = 51) with an MIC<sub>50</sub> and MIC<sub>90</sub> of 0.38 and 0.5 μg/mL by E-test, respectively. Similarly, it demonstrated an MIC<sub>50</sub> and MIC<sub>90</sub> of 0.5 & 1 μg/ml by BMD.</div></div><div><h3>Conclusion</h3><div>The present study demonstrated 100 % susceptibility to levonadifloxacin among the tested isolates of MRSA, highlighting its promising clinical potential for treating infections caused by this organism. Furthermore, the study recorded a concordance of 100 % between disc diffusion and Etest method with BMD, indicating the reliability of these methods.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"53 ","pages":"Article 100788"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142948089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular analysis of acute hemorrhagic conjunctivitis in a tertiary care hospital, Kalburgi district, Karnataka 2023 2023年卡纳塔克邦Kalburgi区一家三级医院急性出血性结膜炎的分子分析

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2025-01-01 DOI: 10.1016/j.ijmmb.2024.100785

Jyothi N. Sanganal , Asharani Shinde , MD Iqbal Ahmed , Sakib Akther Pattassery , Susha Subash , Srinivas Vilasagaram , Praveen Kumar , Marappa Narayana , Ashok Munivenkatappa

{"title":"Molecular analysis of acute hemorrhagic conjunctivitis in a tertiary care hospital, Kalburgi district, Karnataka 2023","authors":"Jyothi N. Sanganal , Asharani Shinde , MD Iqbal Ahmed , Sakib Akther Pattassery , Susha Subash , Srinivas Vilasagaram , Praveen Kumar , Marappa Narayana , Ashok Munivenkatappa","doi":"10.1016/j.ijmmb.2024.100785","DOIUrl":"10.1016/j.ijmmb.2024.100785","url":null,"abstract":"<div><h3>Introduction</h3><div>Viral infections are the predominant cause of acute hemorrhagic conjunctivitis (AHC) across the globe. From July to August of 2023, a large-scale conjunctivitis outbreak was witnessed in India, affecting a large number of individuals. The present study was initiated to identify the causative agent responsible for the AHC outbreak in Kalburgi district of Karnataka state.</div></div><div><h3>Methodology</h3><div>Conjunctival and throat swabs were collected from AHC patients from a tertiary care hospital in Kalburgi from August to September 2023. After total viral RNA extraction from all the samples, reverse transcriptase PCR was performed using 5′ non-coding regions of the enterovirus (EV) genome. The positive samples were further subjected to PCR using EV type-specific primers of Coxsackie A-24 variant (CA24v) and enterovirus-70 (EV-70). EV negative samples were also tested for human adenovirus. Five representative CA24v-positive samples were subjected to partial sequencing and phylogenetic analysis.</div></div><div><h3>Results</h3><div>Thirty-three AHC patient samples were included in the testing and analysis. EV was detected among 54.5 % (18/33) of the patients and all were found to be CA24v, while no EV-70 or human adenovirus was detected. Partial 3C-proteinase sequencing revealed that the CA24v strains from Kalburgi were closely clustered and had 99 % sequence identity with CA24v strains reported from China in 2023. However, the Kalburagi strains were 90.3–93.2 % identical to previously reported Indian strains.</div></div><div><h3>Conclusion</h3><div>To summarize, CA24v was identified as an etiological agent responsible for the recent acute hemorrhagic conjunctivitis outbreak in Kalburgi. The CA24v strain detected was closely related to the currently circulating Asian strains.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"53 ","pages":"Article 100785"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skin colonization by pathogenic bacteria as a risk factor for neonatal sepsis 致病菌在皮肤上定植是新生儿败血症的危险因素。

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2025-01-01 DOI: 10.1016/j.ijmmb.2024.100782

Radha Pandey, Varsha Gupta, Suksham Jain, Neelam Gulati

{"title":"Skin colonization by pathogenic bacteria as a risk factor for neonatal sepsis","authors":"Radha Pandey, Varsha Gupta, Suksham Jain, Neelam Gulati","doi":"10.1016/j.ijmmb.2024.100782","DOIUrl":"10.1016/j.ijmmb.2024.100782","url":null,"abstract":"<div><h3>Background</h3><div>Neonatal sepsis continues to be a leading cause of mortality among the NICU admitted neonates. The most common causative organisms have been proven to be hospital-acquired organisms.</div></div><div><h3>Aims and objectives</h3><div>This study was planned with aim of understanding the pathological colonization of neonatal skin and associated risk factors as well as finding a possible correlation between blood culture isolates and neonatal skin colonizers and their antimicrobial resistance patterns.</div></div><div><h3>Methods</h3><div>This prospective cohort study was conducted at a tertiary care centre in Northern India from January 2021 to June 2022. The study participants were 50 pre-term neonates and 50 term neonates, who were born in our hospital and subsequently admitted to the NICU. Skin swabs, taken from 5 body sites within 24 h of birth and at discharge, were cultured for isolation of pathological bacteria. Neonates were followed-up during their hospital stay for observing any occurrence of blood culture positive sepsis.</div></div><div><h3>Results</h3><div>Out of 100 neonates, 31 pre-term and 28 term neonates were colonized within 24 h of birth while almost all were colonized by discharge. Posterior auricular fossa was the most colonized site. Coagulase Negative <em>Staphylococcus</em> (n = 195) and <em>Escherichia coli</em> (n = 51) were the most common isolates. Risk factors found to be significantly associated with colonization were low birth weight (<2500g), premature rupture of membranes (PROM), invasive mechanical ventilation and positive urine and vaginal cultures of mothers. Neonates with culture positive sepsis also had colonization with MDROs.</div></div><div><h3>Conclusions</h3><div>Neonatal skin colonization and their antimicrobial resistance rates increased over the course of hospital stay, having a possible contribution towards culture positive sepsis.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"53 ","pages":"Article 100782"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of rotavirus vaccine following introduction in the childhood immunization program in Northeast India

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2025-01-01 DOI: 10.1016/j.ijmmb.2025.100794

Thingnam Binita Devi , Heigrujam Rebachandra Singh , Chongtham Shyamsunder Singh , Khuraijam Ranjana Devi , Shakti Laishram

{"title":"Effectiveness of rotavirus vaccine following introduction in the childhood immunization program in Northeast India","authors":"Thingnam Binita Devi , Heigrujam Rebachandra Singh , Chongtham Shyamsunder Singh , Khuraijam Ranjana Devi , Shakti Laishram","doi":"10.1016/j.ijmmb.2025.100794","DOIUrl":"10.1016/j.ijmmb.2025.100794","url":null,"abstract":"<div><h3>Background</h3><div>Rotavirus diarrhoea is a major cause of mortality and morbidity in children under five years of age. The WHO has recommended vaccination against this agent especially in countries with high mortality rates. As India accounts for almost a quarter of worldwide rotaviral diarrhoea cases, the government has introduced indigenously manufactured vaccines in the national immunization schedule which has been implemented in four phases. This study was conducted to assess the effectiveness of the vaccine used in the national immunization program in the state of Manipur in North-east India.</div></div><div><h3>Methods</h3><div>A test negative study design was used comparing rotavirus positive diarrhoeal children to rotavirus negative diarrhoeal children. All children ≤5 years presenting with acute diarrhoea were enrolled in the study and antigen detection enzyme-immuno assay was done to detect presence of rotavirus. All rotavirus positive patients were taken as cases and compared with rotavirus negative controls.</div></div><div><h3>Results</h3><div>63 rotavirus positive cases and 121 rotavirus negative controls were enrolled in the study. Vaccination rate among cases was significantly lower than controls (31.7 % vs 68.6 %, p < .001). Vaccine effectiveness of completed vaccination series was found to be 94 % against rotaviral infection and 98.1 % against severe disease and need for hospitalization.</div></div><div><h3>Conclusion</h3><div>Current rotaviral vaccine used in the national immunization schedule of India is effective in preventing rotaviral diarrhoea in the state of Manipur. Sustained vaccination will reduce the mortality and morbidity of rotaviral diarrhoea in this region.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"53 ","pages":"Article 100794"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A case of Vibrio pelagius in a child with inflammatory bowel disease 炎症性肠病患儿感染伯氏弧菌1例。

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2025-01-01 DOI: 10.1016/j.ijmmb.2024.100783

Akhila R. Mohan , Priya Sree Balaji , Nayannika Lakra , Ramya Srinivasa Rangan , Tamil Selvan , Jharna Mandal

引用次数: 0

Fungal nasal polyposis – A rare case report 真菌性鼻息肉病1例报告。

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2025-01-01 DOI: 10.1016/j.ijmmb.2024.100768

Jaishma Rajni Jeyaraaj, Latha Ragunathan, Kavitha kanniyan, Pramodhini Subramanian, Sherief Shebeena, Mohamed Asarudeen, Jenifer Raj Robin

引用次数: 0

Identification of dengue virus serotype and genotype: A comprehensive study from AIIMS Patna, Bihar

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2025-01-01 DOI: 10.1016/j.ijmmb.2025.100789

Kamal Singh , Md Zeeshan Farooque , Vikram Pal Gandhi, Apurva, Aman Kumar, Asim Sarfraz, Binod Kumar Pati

{"title":"Identification of dengue virus serotype and genotype: A comprehensive study from AIIMS Patna, Bihar","authors":"Kamal Singh , Md Zeeshan Farooque , Vikram Pal Gandhi, Apurva, Aman Kumar, Asim Sarfraz, Binod Kumar Pati","doi":"10.1016/j.ijmmb.2025.100789","DOIUrl":"10.1016/j.ijmmb.2025.100789","url":null,"abstract":"<div><h3>Purpose</h3><div>Dengue virus, a major global health concern, exhibits significant genetic diversity, leading to distinct serotypes and genotypes. Dengue is the second most common disease spread by mosquitoes that infect humans, after malaria. In recent decades, there has also been a shift in the tendencies of virus transmission from urban to peri-urban and rural settings. This research study focuses on the identification and characterization of dengue virus serotypes and genotypes.</div></div><div><h3>Methods</h3><div>In this study, specimens collected in one year were first subjected to serological assay (NS1 and IgM) and subsequently the NS1 positive samples were subjected to identification and characterization of the prevalent dengue virus serotypes and genotypes.</div></div><div><h3>Results</h3><div>During the year 2021, a total of 933serum samples were tested, out of which 75 were found positive for NS1 antigen and 135 were positive for Dengue IgM antibody by ELISA. The dengue serotype specific Real-Time RT-PCR Assay detected all four serotypes in the NS1-positive samples, indicating their presence in this region. Of these, DEN-1 was detected in 4 (7.8 %), DEN-2 in 26 (50.9 %), DEN-3 in 20 (39 %), DEN-4 in 1 (1.9 %). Out of 51Real‐Time RT‐PCR positive samples, 33 were found positive by conventional PCR for genotyping by targeting the capsid-preMembrane (C-prM)region. The genotyping result showed that DENV-1 serotype clustering with GIII, DEN-2 serotype clustering withwide range of genotypes such as IVa, IVb, and IVc, DEN-3 clustering with GIII genotype and DEN-4 serotype showed GIII genotype.</div></div><div><h3>Conclusions</h3><div>This study provides the recent details about the circulating serotypes along with prevalent genotypes in this region.</div></div>","PeriodicalId":13284,"journal":{"name":"Indian Journal of Medical Microbiology","volume":"53 ","pages":"Article 100789"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Triple soil transmitted helminths in a case of aluminium phosphide poisoning 在一起磷化铝中毒案件中，三层土壤传播了蠕虫。

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2025-01-01 DOI: 10.1016/j.ijmmb.2024.100787

Abhishek Mewara, Mamta Thakur, Sana Afreen, Nikita Sharma, Sumeeta Khurana

引用次数: 0

Corrigendum to “Current trends in antimicrobial resistance of ESKAPEEc pathogens from bloodstream infections – Experience of a tertiary care centre in North India” [Indian J. Med. Microbiol. 50 (July–August 2024), 100647] 血流感染 ESKAPEEc 病原体抗菌药耐药性的当前趋势--北印度一家三级护理中心的经验"[《印度医学微生物学杂志》（Indian J. Med. Microbiol. 50 (July-August 2024)，100647）]更正。

IF 1.4 4区医学

Indian Journal of Medical Microbiology Pub Date : 2025-01-01 DOI: 10.1016/j.ijmmb.2024.100662

Veenu Gupta, Menal Gupta, Rama Gupta, Jyoti Chaudhary

引用次数: 0

A rare case report of Listeria ivanovii bacteremia in an elderly patient with uncontrolled diabetes and chronic kidney disease from South India 一例罕见的伊万诺维奇李斯特菌血症在老年患者控制糖尿病和慢性肾脏疾病从印度南部报告。