Indian journal of biochemistry & biophysics最新文献_第3页

Regulatory role of TRPM7 cation channels on neuronal hypoxia model TRPM7阳离子通道在神经元缺氧模型中的调节作用

4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2023-01-01 DOI: 10.56042/ijbb.v60i11.4467

{"title":"Regulatory role of TRPM7 cation channels on neuronal hypoxia model","authors":"","doi":"10.56042/ijbb.v60i11.4467","DOIUrl":"https://doi.org/10.56042/ijbb.v60i11.4467","url":null,"abstract":"Low levels of oxygen have harmful effects on cells especially in neurons because of their vulnerable status for oxygen consumption. Intracellular calcium concentration ([Ca2+]i) is accumulated by several kinds of calcium-permeable channels including the Transient Receptor Potential (TRP) channels. The TRPM7 cation channels are calcium ion (Ca2+) permeable non-selective cation channels belonging to TRP superfamily. The TRPM7 is expressed in different organs of the human body including nervous system components especially in the brain. Some of the TRP channel subtypes are related to oxidative stress and increased oxidative stress triggers channel activity. Recently, TRPM7 cation channels involved in hypoxia. Hence, alterations of [Ca2+]i may be a key factor inTRPM7activity in hypoxia and preventing hypoxic injury of neurons TRPM7 could be a therapeutic target. Therefore, it has been investigated effects of downregulation of TRPM7 cation channels by siRNA applications to induce getting TRPM7 activity down. The role of silencing of TRPM7 cation channels on apoptosis and cell morphology, production of intracellular reactive oxygen species (iROS), mitochondrial membrane depolarization (MMD) levels, enzymatic activity values of caspase 3, 8 and 9 in SH-SY5Y neuronal cells investigated in this study. It has been shown that the downregulation of TRPM7 cation channels may prevent cell death and protect cellular morphology and viability in neuronal cells after chemical hypoxia induction. Decreasing TRPM7 channel activity may also decrease calcium overload and it is a key regulatory function of TRPM7 channels in hypoxic conditions. In conclusion, TRPM7 cation channel antagonists or suppression of the channel expression by genetic manipulations can be a useful and potential therapeutic approach against neuronal hypoxia-related cell death.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135610227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Improving anti-tumour activity with melatonin-stimulated mesenchymal stem cell-derived exosomes in metastatic triple-negative breast cancer 褪黑素刺激间充质干细胞衍生外泌体在转移性三阴性乳腺癌中的抗肿瘤活性

4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2023-01-01 DOI: 10.56042/ijbb.v60i11.1291

{"title":"The Improving anti-tumour activity with melatonin-stimulated mesenchymal stem cell-derived exosomes in metastatic triple-negative breast cancer","authors":"","doi":"10.56042/ijbb.v60i11.1291","DOIUrl":"https://doi.org/10.56042/ijbb.v60i11.1291","url":null,"abstract":"The aim of this study is to increase the bioavailability of melatonin on triple-negative breast cancer (TNBC) cells by loading it into exosomes as well as comparing the therapeutic potentials of melatonin and exosome released from human adipose tissue-derived mesenchymal stem cells. TNBC is one of the most malignant tumours with highly invasive and metastatic features. It is characterized by the absence of estrogen receptor, progesterone receptor and human epidermal growth factor receptor 2. TNBC patients can’t benefit from hormonal or trastuzumab-based therapies targeting these receptors. Exosomes are defined as naturally occurring extracellular vesicles. By enabling the transfer of molecules, exosomes play a role in cancer treatment and dynamic intercellular communication between tumour cells and adjacent stromal compartments. The half-maximum inhibitory concentration IC50 values were 30.38 µg/mL, 40.49 µg/mL and 1.5 mM at the co-administered melatonin and exosome, AT-Exo and Mel groups, respectively, for 48 h. The percentage of late-stage apoptotic induction was found to be 6.3%, 4.1% and 4.6% for TNBC exposed to co-administered melatonin and exosome (2.5 mM +100 µg/mL Mel/Exo) for 24 h, 48 h, 72 h, respectively. In conclusion, the coexistence of exosomes and melatonin represents a promising therapeutic tool that can interfere with key molecular processes such as cytotoxicity and apoptosis cascade in TNBC.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135610230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic analysis of Substantia nigra proteins in STZ-induced Type-II diabetic rats: A possible link with Parkinson’s disease stz诱导的ii型糖尿病大鼠黑质蛋白的蛋白质组学分析:与帕金森病的可能联系

4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2023-01-01 DOI: 10.56042/ijbb.v60i10.214

{"title":"Proteomic analysis of Substantia nigra proteins in STZ-induced Type-II diabetic rats: A possible link with Parkinson’s disease","authors":"","doi":"10.56042/ijbb.v60i10.214","DOIUrl":"https://doi.org/10.56042/ijbb.v60i10.214","url":null,"abstract":"Type-II diabetes mellitus (TIIDM) is a metabolic disorder characterized by high level of glucose in blood due to high secretion of glucose from peripheral tissues, low secretion of insulin or dysfunction of insulin. Parkinson’s disease (PD) is a neurodegenerative disorder characterized by loss of Dopamine in the Substantia nigra of midbrain leading to cause motor dysfunction in affected people. TIIDM and PD have associations with each other as the individuals suffering from TIIDM are at more risk to develop PD in old age. The present study has analyzed eight (08) differentially expressed proteins in the Substantia nigra of TIIDM rat’s brain by using Nano-LC-MS/MS method. Nano-LC-MS/MS is highly recommended technique for the identification & quantification of proteins. RPS27a, PSMC1, PSMa4, ATP8, ATP5f1d and CALM3 were down-regulated while PSMa3 and PRKACa were up-regulated in rat’s brain. These differentially regulated proteins were further analyzed and found to be involved in the oxidative stress, mitochondrial dysfunction, Dopamine pathway, dysfunctional insulin signaling pathway, Ubiquitin regulatory pathways, and Ca2+ signaling pathways of both TIIDM and PD. In conclusion, the current study proposed a link between TIIDM and PD through primary estimated involvement of these proteins in both disorders. However, more in-depth molecular and proteomics studies are needed to be carried out for the possible expression of current proteins as target in the treatment and or prevention of PD in TIIDM patients.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135799260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of active transport of monovalent cation across the animal cell plasma membranes by cytosolic regulatory proteins. 细胞质调节蛋白对动物细胞膜上单价阳离子主动转运的调控。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2013-09-04 DOI: 10.5281/ZENODO.7095

Ray Tushar, Ranjan India Chakrabarti

引用次数: 7

Expression and role of miR-34a in bladder cancer. miR-34a在膀胱癌中的表达及作用

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2012-12-15 DOI: 10.3760/CMA.J.ISSN.1000-6702.2012.12.017

Wei Wang, Tan Li, G. Han, Ying Li, Li-hua Shi, Hui Li

引用次数: 16

Dietary cholesterol and estrogen administration elevate brain apolipoprotein E in mice by different mechanisms. 膳食胆固醇和雌激素通过不同的机制提高小鼠脑载脂蛋白E。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2008-10-01 DOI: 10.5214/93

N. Srivastava, M. Averna, R. Srivastava

{"title":"Dietary cholesterol and estrogen administration elevate brain apolipoprotein E in mice by different mechanisms.","authors":"N. Srivastava, M. Averna, R. Srivastava","doi":"10.5214/93","DOIUrl":"https://doi.org/10.5214/93","url":null,"abstract":"Apolipoprotein (apo) E plays an important role in the whole body cholesterol homeostasis. Recent studies suggest that it may also be involved in the local cholesterol transport in the brain, and influence the pathogenesis of Alzheimer's disease (AD) by interacting with the beta-amyloid protein and brain lipoprotein receptors. Since apoE expression is highest in the brain, next only to the liver and associated with the pathogenesis of AD, we hypothesized that dietary and hormonal intervention, known to regulate hepatic apoE expression may also regulate brain apoE and thereby influence local cholesterol transport. To test this hypothesis, groups of male C57BL mice were fed either regular rodent chow or high fat (HF) and high cholesterol enriched diet for 3 weeks. In a separate study, groups of male mice were administered pharmacological doses of 17-beta estradiol for 5 consecutive days and sacrificed on the 6th day. As expected, HF diet elevated liver apoE mRNA and apoE synthesis. Similar to liver, brain apoE mRNA and synthesis also increased, following HF feeding. Estradiol administration increased liver apoE synthesis without affecting apoE mRNA. Interestingly, estradiol administration also increased the brain apoE synthesis, but without altering the brain apoE mRNA. These studies suggested that dietary cholesterol and estrogen administration elevated the brain apoE by different mechanisms.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2008-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70560534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

About this mini symposium. 关于这个小型研讨会。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2002-08-01 DOI: 10.5648/jjiao.30.24

M. Gupta

引用次数: 7

Isolation and characterization of NADP+-linked isocitrate dehydrogenase of germinating pea seeds (Pisum sativum). 豌豆种子（Pisum sativum）萌发过程中与 NADP+ 链接的异柠檬酸脱氢酶的分离和特征描述。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2001-10-01

P K Srivastava, D S Singh

引用次数: 0

Purification and properties of antiviral proteins from the leaves of Bougainvillea xbuttiana. 九重葛叶抗病毒蛋白的纯化和特性。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2001-10-01

S Narwal, A Balasubrahmanyam, M L Lodha, H C Kapoor

引用次数: 0

Role of metal ions in coupling between chemical catalysis and conformational changes in ATP synthase. 金属离子在 ATP 合酶的化学催化和构象变化之间的耦合中的作用。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2000-12-01

Y Hochman, X M Gong, Y Lifshitz, C Carmeli

{"title":"Role of metal ions in coupling between chemical catalysis and conformational changes in ATP synthase.","authors":"Y Hochman, X M Gong, Y Lifshitz, C Carmeli","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Isolated chloroplast ATP synthase (CF0F1) was used for determination of the structure-function relation by measuring the effect of divalent metal ions on the properties of ATPase. Mg2+ ions were more efficient catalysts than Ca2+ ions as indicated by Kcat/Km of 55.2 and 5.4, respectively. Other activity parameters related to binding, such as the Km of MATP and Ki of MADP, indicated a stronger binding in the presence of Mg2+ as seen from a Mg2+/Ca2+ ratio of 2.8 and 3.8, respectively. Strong binding of Ca2+ ions with a Kd of 0.03 +/- 00.6 microM-1 was detected only in the presence of ADP probably because of the positive interactive effect of CaADP as indicated in the inhibition properties. Mg2+ ions were more efficient catalysts also in other forms of the enzyme such as in the thylakoid membrane, in isolated CF0F1 and in CF1. The Mg2+/Ca2+ ratio of Kcat/Km was 5.3, 10.2 and 1.5 for the thylakoid membrane enzyme, the isolated CF0F1 and the soluble CF1 respectively. This indicated that Ca2+ ions became less efficient catalysts in the more intact and integrated enzyme while Mg2+ ions were as efficient in all forms of the enzyme. Unlike Mg2+, Ca2+ ions also did not support proton-coupled ATP synthesis and ATP driven proton pumping. It is suggested that the differences in the ligand structure of these two ions might be the reason for the differential function. An average 0.3 A shorter bond length of octahedral first coordination in Ca2+ ions caused a weaker binding of CaATP than that of MgATP. The effect of differential binding is discussed in relation to the binding of the transition state intermediate and to the rate of product release.</p>","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2000-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138802238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0