Indian journal of biochemistry & biophysics最新文献_第3页

Regulatory role of TRPM7 cation channels on neuronal hypoxia model TRPM7阳离子通道在神经元缺氧模型中的调节作用

4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2023-01-01 DOI: 10.56042/ijbb.v60i11.4467

{"title":"Regulatory role of TRPM7 cation channels on neuronal hypoxia model","authors":"","doi":"10.56042/ijbb.v60i11.4467","DOIUrl":"https://doi.org/10.56042/ijbb.v60i11.4467","url":null,"abstract":"Low levels of oxygen have harmful effects on cells especially in neurons because of their vulnerable status for oxygen consumption. Intracellular calcium concentration ([Ca2+]i) is accumulated by several kinds of calcium-permeable channels including the Transient Receptor Potential (TRP) channels. The TRPM7 cation channels are calcium ion (Ca2+) permeable non-selective cation channels belonging to TRP superfamily. The TRPM7 is expressed in different organs of the human body including nervous system components especially in the brain. Some of the TRP channel subtypes are related to oxidative stress and increased oxidative stress triggers channel activity. Recently, TRPM7 cation channels involved in hypoxia. Hence, alterations of [Ca2+]i may be a key factor inTRPM7activity in hypoxia and preventing hypoxic injury of neurons TRPM7 could be a therapeutic target. Therefore, it has been investigated effects of downregulation of TRPM7 cation channels by siRNA applications to induce getting TRPM7 activity down. The role of silencing of TRPM7 cation channels on apoptosis and cell morphology, production of intracellular reactive oxygen species (iROS), mitochondrial membrane depolarization (MMD) levels, enzymatic activity values of caspase 3, 8 and 9 in SH-SY5Y neuronal cells investigated in this study. It has been shown that the downregulation of TRPM7 cation channels may prevent cell death and protect cellular morphology and viability in neuronal cells after chemical hypoxia induction. Decreasing TRPM7 channel activity may also decrease calcium overload and it is a key regulatory function of TRPM7 channels in hypoxic conditions. In conclusion, TRPM7 cation channel antagonists or suppression of the channel expression by genetic manipulations can be a useful and potential therapeutic approach against neuronal hypoxia-related cell death.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135610227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Improving anti-tumour activity with melatonin-stimulated mesenchymal stem cell-derived exosomes in metastatic triple-negative breast cancer 褪黑素刺激间充质干细胞衍生外泌体在转移性三阴性乳腺癌中的抗肿瘤活性

4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2023-01-01 DOI: 10.56042/ijbb.v60i11.1291

{"title":"The Improving anti-tumour activity with melatonin-stimulated mesenchymal stem cell-derived exosomes in metastatic triple-negative breast cancer","authors":"","doi":"10.56042/ijbb.v60i11.1291","DOIUrl":"https://doi.org/10.56042/ijbb.v60i11.1291","url":null,"abstract":"The aim of this study is to increase the bioavailability of melatonin on triple-negative breast cancer (TNBC) cells by loading it into exosomes as well as comparing the therapeutic potentials of melatonin and exosome released from human adipose tissue-derived mesenchymal stem cells. TNBC is one of the most malignant tumours with highly invasive and metastatic features. It is characterized by the absence of estrogen receptor, progesterone receptor and human epidermal growth factor receptor 2. TNBC patients can’t benefit from hormonal or trastuzumab-based therapies targeting these receptors. Exosomes are defined as naturally occurring extracellular vesicles. By enabling the transfer of molecules, exosomes play a role in cancer treatment and dynamic intercellular communication between tumour cells and adjacent stromal compartments. The half-maximum inhibitory concentration IC50 values were 30.38 µg/mL, 40.49 µg/mL and 1.5 mM at the co-administered melatonin and exosome, AT-Exo and Mel groups, respectively, for 48 h. The percentage of late-stage apoptotic induction was found to be 6.3%, 4.1% and 4.6% for TNBC exposed to co-administered melatonin and exosome (2.5 mM +100 µg/mL Mel/Exo) for 24 h, 48 h, 72 h, respectively. In conclusion, the coexistence of exosomes and melatonin represents a promising therapeutic tool that can interfere with key molecular processes such as cytotoxicity and apoptosis cascade in TNBC.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135610230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic analysis of Substantia nigra proteins in STZ-induced Type-II diabetic rats: A possible link with Parkinson’s disease stz诱导的ii型糖尿病大鼠黑质蛋白的蛋白质组学分析:与帕金森病的可能联系

4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2023-01-01 DOI: 10.56042/ijbb.v60i10.214

{"title":"Proteomic analysis of Substantia nigra proteins in STZ-induced Type-II diabetic rats: A possible link with Parkinson’s disease","authors":"","doi":"10.56042/ijbb.v60i10.214","DOIUrl":"https://doi.org/10.56042/ijbb.v60i10.214","url":null,"abstract":"Type-II diabetes mellitus (TIIDM) is a metabolic disorder characterized by high level of glucose in blood due to high secretion of glucose from peripheral tissues, low secretion of insulin or dysfunction of insulin. Parkinson’s disease (PD) is a neurodegenerative disorder characterized by loss of Dopamine in the Substantia nigra of midbrain leading to cause motor dysfunction in affected people. TIIDM and PD have associations with each other as the individuals suffering from TIIDM are at more risk to develop PD in old age. The present study has analyzed eight (08) differentially expressed proteins in the Substantia nigra of TIIDM rat’s brain by using Nano-LC-MS/MS method. Nano-LC-MS/MS is highly recommended technique for the identification & quantification of proteins. RPS27a, PSMC1, PSMa4, ATP8, ATP5f1d and CALM3 were down-regulated while PSMa3 and PRKACa were up-regulated in rat’s brain. These differentially regulated proteins were further analyzed and found to be involved in the oxidative stress, mitochondrial dysfunction, Dopamine pathway, dysfunctional insulin signaling pathway, Ubiquitin regulatory pathways, and Ca2+ signaling pathways of both TIIDM and PD. In conclusion, the current study proposed a link between TIIDM and PD through primary estimated involvement of these proteins in both disorders. However, more in-depth molecular and proteomics studies are needed to be carried out for the possible expression of current proteins as target in the treatment and or prevention of PD in TIIDM patients.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135799260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bile salt incorporated polypyrrole thin film for ethanol sensing. 胆盐掺入聚吡咯薄膜用于乙醇传感。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2015-04-01

Partha P D Sharma, D Sarkar

{"title":"Bile salt incorporated polypyrrole thin film for ethanol sensing.","authors":"Partha P D Sharma, D Sarkar","doi":"","DOIUrl":"","url":null,"abstract":"Polypyrrole (PPy)-bile salt composite was used for sensing ethanol vapor. PPy was synthesized by interface polymerization for subsequent fabrication of thin film of its composite with bile salt, by in-situ co-dispersion method and then exposed to ethanol vapour. Sensing was visualized through changes in morphological, structural and optical characterizations. The ethanol exposed film showed larger agglomeration as revealed in its surface morphology on scanning electron microscope (SEM) and greater crystallinity as seen through X-Ray diffraction (XRD). Fourier transform infra red (FTIR) and nuclear magnetic resonance spectroscopy (NMR) of the ethanol incorporated film also gave signature of the presence of bile salt and alcohol. Alcohol incorporation pattern resulted in increase in electrical conductance from 7.08539 x 10(-5) mA/V to 8.0356 x 10(-5) mA/V, as determined from current voltage characterizations. Average molecular weight (M(n)) obtained from gel permeation chromatography changed from 6160 to 10300 on ethanol intake. Photoluminescence (PL) intensity was quenched and the PL peak shifted from 430 to 409 on ethanol exposure. Changes in morphological, structural, optical and electrical properties of the composite on ethanol exposure showed its prospective application for sensing ethanol.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"52 2","pages":"203-8"},"PeriodicalIF":1.4,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33425153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of activity of OsDmc1A recombinase of rice (Oryza sativa) in presence of Ca2+ and Mg2+ ions. Ca2+和Mg2+存在下水稻OsDmc1A重组酶活性的比较

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2015-04-01

Rajani Kant Chittela, Michael Melzer, Jayashree Krishna Sainis

{"title":"Comparison of activity of OsDmc1A recombinase of rice (Oryza sativa) in presence of Ca2+ and Mg2+ ions.","authors":"Rajani Kant Chittela, Michael Melzer, Jayashree Krishna Sainis","doi":"","DOIUrl":"","url":null,"abstract":"Recombinases are known to play an important role in the homology search and strand exchange during meiosis as well as homologous recombination (HR)-mediated DNA repair specifically require Mg2+ ion for their activity. The Ca2+ has been shown to stimulate the strand exchange activity of hDmc1 and ScDmc1 by forming the extended filaments on DNA. Oryza sativa disrupted meiotic cDNA1A (OsDmc1A), a homologue of yeast and human Dmc1 from rice shows the hallmark functions of recombinase. Here, we report the effects of Ca2+ and Mg2+ on OsDmc1A activity from rice (Oryza sativa). OsDmc1A showed a concentration-dependent binding with both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) substrates in presence of Mg2+ or Ca2+. The ssDNA and dsDNA binding activities, as well as renaturation activity of OsDmc1A were similar in the presence of Ca2+ or Mg2+. Increasing the Ca2+ or Mg2+ increased the DNA binding, renaturation and strand exchange of OsDmc1A. But, OsDmc1A showed only a slight stimulation of strand exchange activity in presence of Ca2+, when compared the activity in presence of Mg2+. Electron microscopy showed that OsDmc1A formed ring-like structures in presence of Mg2+ or Ca2+. However, OsDmc1A formed filament like structures with both ss and dsDNA in presence of Mg2+ or Ca2+. Taken together, Ca2+ did not affect OsDmc1A recombinase activity significantly.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"52 2","pages":"161-8"},"PeriodicalIF":1.4,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33425762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microarray analysis of differentially expressed genes regulating lipid metabolism during melanoma progression. 微阵列分析黑色素瘤进展过程中调节脂质代谢的差异表达基因。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2015-04-01

Venil N Sumantran, Pratik Mishra, N Sudhakar

{"title":"Microarray analysis of differentially expressed genes regulating lipid metabolism during melanoma progression.","authors":"Venil N Sumantran, Pratik Mishra, N Sudhakar","doi":"","DOIUrl":"","url":null,"abstract":"A new hallmark of cancer involves acquisition of a lipogenic phenotype which promotes tumorigenesis. Little is known about lipid metabolism in melanomas. Therefore, we used BRB (Biometrics Research Branch) class comparison tool with multivariate analysis to identify differentially expressed genes in human cutaneous melanomas, compared with benign nevi and normal skin derived from the microarray dataset (GDS1375). The methods were validated by identifying known melanoma biomarkers (CITED1, FGFR2, PTPRF, LICAM, SPP1 and PHACTR1) in our results. Eighteen genes regulating metabolism of fatty acids, lipid second messengers and gangliosides were 2-9 fold upregulated in melanomas of GDS-1375. Out of the 18 genes, 13 were confirmed by KEGG pathway analysis and 10 were also significantly upregulated in human melanoma cell lines of NCI-60 Cell Miner database. Results showed that melanomas upregulated PPARGC1A transcription factor and its target genes regulating synthesis of fatty acids (SCD) and complex lipids (FABP3 and ACSL3). Melanoma also upregulated genes which prevented lipotoxicity (CPT2 and ACOT7) and regulated lipid second messengers, such as phosphatidic acid (AGPAT-4, PLD3) and inositol triphosphate (ITPKB, ITPR3). Genes for synthesis of pro-tumorigenic GM3 and GD3 gangliosides (UGCG, HEXA, ST3GAL5 and ST8SIA1) were also upregulated in melanoma. Overall, the microarray analysis of GDS-1375 dataset indicated that melanomas can become lipogenic by upregulating genes, leading to increase in fatty acid metabolism, metabolism of specific lipid second messengers, and ganglioside synthesis.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"52 2","pages":"125-31"},"PeriodicalIF":1.4,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33425757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and functional analysis of chitinase gene family in wheat (Triticum aestivum). 小麦几丁质酶基因家族的结构与功能分析。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2015-04-01

A K Mishra, Bharati Pandey, Chetna Tyagi, Ohika Chakraborty, Amrender Kumar, A K Jain

{"title":"Structural and functional analysis of chitinase gene family in wheat (Triticum aestivum).","authors":"A K Mishra, Bharati Pandey, Chetna Tyagi, Ohika Chakraborty, Amrender Kumar, A K Jain","doi":"","DOIUrl":"","url":null,"abstract":"Chitinases are the hydrolytic enzymes which protect plants against pathogen attack. However, the precise role of chitinases in disease resistance has not been explored in wheat. In the present study, in silico approach, including secondary structure analysis, detailed signature pattern study, cis-acting regulatory elements survey, evolutionary trends and three-dimensional molecular modeling was used for different chitinase classes of wheat (Triticum aestivum). Homology modeling of class I, II, IV and 3 chitinase proteins was performed using the template crystal structure. The model structures were further refined by molecular mechanics methods using different tools, such as Procheck, ProSA and Verify3D. Secondary structure studies revealed greater percentage of residues forming a helix conformation with specific signature pattern, similar to casein kinase II phosphorylation site, amidation site, N-myristoylation (N-MYR) site and protein kinase C phoshorylation site. The expression profile suggested that wheat chitinase gene was highly expressed in cell culture and callus. We found that wheat chitinases showed more functional similarity with rice and barley. The results provide insight into the evolution of the chitinase family, constituting a diverse array of pathogenesis-related proteins. The study also provides insight into the possible binding sites of chitinase proteins and may further enhance our knowledge of fungal resistance mechanism in plants.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"52 2","pages":"169-78"},"PeriodicalIF":1.4,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33425763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermostable, alkaline and detergent-tolerant lipase from a newly isolated thermophilic Bacillus stearothermophilus. 从新分离的嗜热脂肪嗜热芽孢杆菌中分离出耐热、耐碱性和耐洗涤剂的脂肪酶。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2015-04-01

Abir Ben Bacha, Nadine M S Moubayed, Islam Abid

{"title":"Thermostable, alkaline and detergent-tolerant lipase from a newly isolated thermophilic Bacillus stearothermophilus.","authors":"Abir Ben Bacha, Nadine M S Moubayed, Islam Abid","doi":"","DOIUrl":"","url":null,"abstract":"Lipases are the enzymes of choice for laundry detergent industries, owing to their triglyceride removing ability from the soiled fabric, which eventually reduces the usage of phosphate-based chemical cleansers in the detergent formulation. In this study, a novel thermo-alkaline lipase-producing strain identified as Bacillus stearothermophilus was isolated from the soil samples of olive oil mill. Enhanced lipase production was observed at 55 degrees C, pH 11 and after 48 h of incubation. Among the substrates tested, xylose (a carbon source), peptone (a nitrogen source) and olive oil at a concentration of 1% were suitable substrates for enhancing lipase production. MgSO4 and Tween-80 were suitable substrates for maximizing lipase production. The enzyme was purified to homogeneity by a single CM-Sephadex column chromatography and revealed molecular mass of 67 kDa. The enzyme (BL1) was active over a wide range of pH from 9.0 to 13.0, with an optimum at pH 11.0, exhibited maximal activity at 55 degreesC and retained more than 70% of its activity after incubation at 70 degrees C or pH 13 for 0.5 h or 24 h, respectively. The enzyme hydrolyzed both short and long-chain triacylglycerols at comparable rates. BL1 was studied in a preliminary evaluation for use in detergent formulation solutions. This novel lipase showed extreme stability towards non-ionic and anionic surfactants after pre-incubation for 1 h at 40 degrees C, and good stability towards oxidizing agents. Additionally, the enzyme showed excellent stability and compatibility with various commercial detergents, suggesting its potential as an additive in detergent formulations.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"52 2","pages":"179-88"},"PeriodicalIF":1.4,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33425764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of covalent attachment of neomycin on conformational and aggregation properties of catalase. 新霉素共价附着对过氧化氢酶构象和聚集性质的影响。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2015-04-01

S Hashemnia, Z Mokhtari, J Tashkhourian, A A Moosavi-Movahedi

{"title":"Effect of covalent attachment of neomycin on conformational and aggregation properties of catalase.","authors":"S Hashemnia, Z Mokhtari, J Tashkhourian, A A Moosavi-Movahedi","doi":"","DOIUrl":"","url":null,"abstract":"The carboxylic groups of glutamic acid and aspartic acid residues of catalase (CAT) were chemically modified using the treatment of the enzyme with 1-ethyl-3-(3'-dimethylamino) carbodiimide hydrochloride (EDC) and neomycin. The effect of covalent attachment of neomycin on the enzymatic activity, conformational and aggregation properties of CAT was investigated. The modification of CAT with different concentrations of neomycin showed two different types of behavior, depending up on the concentration range of neomycin. In the concentration range from 0.0 to 5.2 mM, neomycin-modified CAT, compared to the native enzyme exhibited higher a-helix content, reduced surface hydrophobicity, little enhancement in CAT activity and a better protection against thermal aggregation, whereas at concentrations greater than 5.2 mM, the modified enzyme exhibited a significant decrease in CAT activity and an increase in random coil content which may result in disorder in the protein structure and increase in thermal aggregation. This modification is a rapid and simple approach to investigate the role of aspartate and glutamate residues in the structure, function and folding of CAT.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"52 2","pages":"189-95"},"PeriodicalIF":1.4,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33425151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimation of the use of fibrin and collagen membranes as carriers for platelet-derived growth factor-BB (PDGF-BB) in the presence of amoxicillin. 估计在阿莫西林存在下，纤维蛋白和胶原膜作为血小板衍生生长因子- bb (PDGF-BB)载体的使用。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2015-04-01

Marta Michalska, Marcin Kozakiewicz, Andrzej Bodek, Kazimiera Henryka Bodek

{"title":"Estimation of the use of fibrin and collagen membranes as carriers for platelet-derived growth factor-BB (PDGF-BB) in the presence of amoxicillin.","authors":"Marta Michalska, Marcin Kozakiewicz, Andrzej Bodek, Kazimiera Henryka Bodek","doi":"","DOIUrl":"","url":null,"abstract":"The effect of homogeneous fibrin (Fb), collagen (Coll) and composite fibrin-heparin (Fb-Hp), fibrin-collagen (Fb-Coll) membranes on in vitro release of platelet-derived growth factor (PDGF-BB) was evaluated in the presence or absence of amoxicillin using of the ELISA immunoassay test. Amoxicillin concentration was determined spectrophotometrically at 272 nm. The process of the PDGF-BB growth factor and amoxicillin release from the studied membranes was of a two-phase nature in the majority of the systems analysed. The PDGF-BB was released in the highest amount from the Coll membrane (M7) without the presence of amoxicillin--546.2 ± 7.47 pg, t0.5 = 0.88 h and 202.5 ± 6.83 pg, t0.5 = 26.65 h during the first phase and second phase, respectively. The lowest PDGF-BB release was observed from composite M4 (Fb-Hp) membrane--5.88 ± 0.81 pg, t0.5 = 1.69 h; and 110.2 ± 6.48 pg, t0.5 = 855.6 h during first and second phase respectively. An optimal release of amoxicillin was observed in the case of the composite M6 (Fb-Coll) membrane--only in the second phase: 64.2 ± 7.8 μg, t0.5 = 83.5 h. The lowest and delayed amoxicillin release was achieved for M4 membrane (approx. 17.1 ± 1.12 μg, t0.5 = 46.5 h). The results of the PDGF-BB release and amoxicillin from membranes indicated a correlation between the level of release and composition of the film. Our results suggested that fibrin and collagen membranes may be beneficial to enhance periodontal bone regeneration.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"52 2","pages":"196-202"},"PeriodicalIF":1.4,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33425152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0