Rajani Kant Chittela, Michael Melzer, Jayashree Krishna Sainis
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Comparison of activity of OsDmc1A recombinase of rice (Oryza sativa) in presence of Ca2+ and Mg2+ ions.
Recombinases are known to play an important role in the homology search and strand exchange during meiosis as well as homologous recombination (HR)-mediated DNA repair specifically require Mg2+ ion for their activity. The Ca2+ has been shown to stimulate the strand exchange activity of hDmc1 and ScDmc1 by forming the extended filaments on DNA. Oryza sativa disrupted meiotic cDNA1A (OsDmc1A), a homologue of yeast and human Dmc1 from rice shows the hallmark functions of recombinase. Here, we report the effects of Ca2+ and Mg2+ on OsDmc1A activity from rice (Oryza sativa). OsDmc1A showed a concentration-dependent binding with both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) substrates in presence of Mg2+ or Ca2+. The ssDNA and dsDNA binding activities, as well as renaturation activity of OsDmc1A were similar in the presence of Ca2+ or Mg2+. Increasing the Ca2+ or Mg2+ increased the DNA binding, renaturation and strand exchange of OsDmc1A. But, OsDmc1A showed only a slight stimulation of strand exchange activity in presence of Ca2+, when compared the activity in presence of Mg2+. Electron microscopy showed that OsDmc1A formed ring-like structures in presence of Mg2+ or Ca2+. However, OsDmc1A formed filament like structures with both ss and dsDNA in presence of Mg2+ or Ca2+. Taken together, Ca2+ did not affect OsDmc1A recombinase activity significantly.
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Started in 1964, this journal publishes original research articles in the following areas: structure-function relationships of biomolecules; biomolecular recognition, protein-protein and protein-DNA interactions; gene-cloning, genetic engineering, genome analysis, gene targeting, gene expression, vectors, gene therapy; drug targeting, drug design; molecular basis of genetic diseases; conformational studies, computer simulation, novel DNA structures and their biological implications, protein folding; enzymes structure, catalytic mechanisms, regulation; membrane biochemistry, transport, ion channels, signal transduction, cell-cell communication, glycobiology; receptors, antigen-antibody binding, neurochemistry, ageing, apoptosis, cell cycle control; hormones, growth factors; oncogenes, host-virus interactions, viral assembly and structure; intermediary metabolism, molecular basis of disease processes, vitamins, coenzymes, carrier proteins, toxicology; plant and microbial biochemistry; surface forces, micelles and microemulsions, colloids, electrical phenomena, etc. in biological systems. Solicited peer reviewed articles on contemporary Themes and Methods in Biochemistry and Biophysics form an important feature of IJBB.
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