Proteomic analysis of Substantia nigra proteins in STZ-induced Type-II diabetic rats: A possible link with Parkinson’s disease

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-01-01 DOI:10.56042/ijbb.v60i10.214

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引用次数: 0

Abstract

Type-II diabetes mellitus (TIIDM) is a metabolic disorder characterized by high level of glucose in blood due to high secretion of glucose from peripheral tissues, low secretion of insulin or dysfunction of insulin. Parkinson’s disease (PD) is a neurodegenerative disorder characterized by loss of Dopamine in the Substantia nigra of midbrain leading to cause motor dysfunction in affected people. TIIDM and PD have associations with each other as the individuals suffering from TIIDM are at more risk to develop PD in old age. The present study has analyzed eight (08) differentially expressed proteins in the Substantia nigra of TIIDM rat’s brain by using Nano-LC-MS/MS method. Nano-LC-MS/MS is highly recommended technique for the identification & quantification of proteins. RPS27a, PSMC1, PSMa4, ATP8, ATP5f1d and CALM3 were down-regulated while PSMa3 and PRKACa were up-regulated in rat’s brain. These differentially regulated proteins were further analyzed and found to be involved in the oxidative stress, mitochondrial dysfunction, Dopamine pathway, dysfunctional insulin signaling pathway, Ubiquitin regulatory pathways, and Ca2+ signaling pathways of both TIIDM and PD. In conclusion, the current study proposed a link between TIIDM and PD through primary estimated involvement of these proteins in both disorders. However, more in-depth molecular and proteomics studies are needed to be carried out for the possible expression of current proteins as target in the treatment and or prevention of PD in TIIDM patients.

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stz诱导的ii型糖尿病大鼠黑质蛋白的蛋白质组学分析:与帕金森病的可能联系

ii型糖尿病(type2 diabetes mellitus, TIIDM)是由于外周组织分泌葡萄糖过多、胰岛素分泌不足或胰岛素功能障碍导致血中葡萄糖水平升高的代谢性疾病。帕金森病(PD)是一种神经退行性疾病，其特征是中脑黑质多巴胺缺失，导致患者运动功能障碍。TIIDM和PD相互关联，因为患有TIIDM的个体在老年时患PD的风险更大。本研究采用纳米lc -MS/MS方法分析了TIIDM大鼠脑黑质中8(08)种差异表达蛋白。纳米lc -MS/MS是一种非常值得推荐的鉴定技术。蛋白质的定量。RPS27a、PSMC1、PSMa4、ATP8、ATP5f1d和CALM3下调，PSMa3和PRKACa上调。进一步分析这些差异调节蛋白，发现它们参与了TIIDM和PD的氧化应激、线粒体功能障碍、多巴胺通路、功能失调的胰岛素信号通路、泛素调节通路和Ca2+信号通路。总之，目前的研究通过初步估计这些蛋白在两种疾病中的参与，提出了TIIDM和PD之间的联系。然而，对于当前蛋白作为靶点在治疗或预防TIIDM患者PD中的可能表达，还需要进行更深入的分子和蛋白质组学研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.