Indian journal of biochemistry & biophysics最新文献_第4页

Angiotensin II inhibits Na+/K+ATPase activity in pulmonary artery smooth muscle cells via glutathionylation and with the involvement of a 15.6 kDa inhibitor protein. 血管紧张素II通过谷胱甘肽化抑制肺动脉平滑肌细胞Na+/K+ atp酶活性，并参与15.6 kDa抑制蛋白。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2015-04-01

Sayed Modinur Rahaman, Kuntal Dey, Tapati Chakraborti, Sajal Chakraborti

引用次数: 0

Vascular endothelial growth factor insertion/deletion gene polymorphism in West Indian patients of type 2 diabetes and diabetic nephropathy. 西印度群岛2型糖尿病和糖尿病肾病患者血管内皮生长因子插入/缺失基因多态性

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2015-04-01

Brijesh Dabhi, Kinnari N Mistry, Hitesh Patel, Sanjay Lal

{"title":"Vascular endothelial growth factor insertion/deletion gene polymorphism in West Indian patients of type 2 diabetes and diabetic nephropathy.","authors":"Brijesh Dabhi, Kinnari N Mistry, Hitesh Patel, Sanjay Lal","doi":"","DOIUrl":"","url":null,"abstract":"Diabetic nephropathy (DN) is a major cause of morbidity and mortality in diabetes. Vascular endothelial growth factor (VEGF) is a potent multi-functional cytokine which plays a key role in the pathogenesis of DN. In this study, we evaluated the possible association of the VEGF gene (I/D) polymorphisms with DN in type 2 diabetes patients in West Indian population. Genotyping (I/D) of the VEGF gene polymorphism was done by the polymerase chain reaction. A total of 103 patients with type 2 diabetes, 102 patients with DN, 108 patients with non-diabetic nephropathy and 143 healthy controls were genotyped. The frequency of VEGF genotype distribution and biochemical parameters like creatinine and HbA1c were compared in diabetic, diabetic nephropathy, non diabetic nephropathy and control groups. We found significant difference in creatinine level in DN and NDN groups on comparison with control group. Our study suggests that I/D polymorphism in the promoter region of the VEGF gene is not associated with DN in type 2 diabetes patients, but might have a role in development of non-diabetic nephropathy.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"52 2","pages":"209-12"},"PeriodicalIF":1.4,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33425154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Umbilical cord mesenchymal stem cells in neurological disorders: A clinical study. 脐带间充质干细胞治疗神经系统疾病的临床研究。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2015-04-01

Xingyu Miao, Xiaoying Wu, Wei Shi

{"title":"Umbilical cord mesenchymal stem cells in neurological disorders: A clinical study.","authors":"Xingyu Miao, Xiaoying Wu, Wei Shi","doi":"","DOIUrl":"","url":null,"abstract":"We investigated the intrathecally administrated unbilical cord mesenchymal stem cells (UC-MSCs) by lumbar puncture and assessed the technical difficulties and effects in various neurological conditions. One hundred patients underwent subarachnoid placement of UC-MSCs between December 2006 and May 2010 in the Affiliated Hospital of Medicine. Technical difficulties in patients in the form of localization of subarachnoid space, number of attempts, and post-procedural complications were evaluated. Functional evaluation was done using Hauser Ambulation Index (HAI) by the stem cell transplant team on a regular basis. All patients were followed-up for more than 1 yr after the treatment. Clinical symptoms, related biochemical index and photographic examinations were observed regularly. We encountered technical difficulties in 31 patients (31%) in the form of general anesthesia supplementation and difficulty localizing the lumbar space. Side effects (headache, low-grade fever, low back pain and lower limb pain) were observed in 22 (22%) patients, which were treated with symptomatic therapy within 48 h. One year after the treatment, functional indices improved in 47 patients (47%): 12 patients with spinal cord injury, 11 patients with cerebral palsy, 9 patients with post-traumatic brain syndrome, 9 patients with post-brain infarction syndrome, 3 patients with spinocerebellar ataxias, and 3 patients with motor neuron disease. In conclusion, intrathecal administration of UC-MSCs is a safe and effective way to treat neurological disorders. Our encouraging results of intrathecal administration of UC-MSCs indicate the potential of restoration of lost tissue and improvement of function in patients with profound neurological defects and inefficient conventional cure. These data support expanded double-blind, placebo-controlled studies for this treatment modality.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"52 2","pages":"140-6"},"PeriodicalIF":1.4,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33425759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research Workshop on Diagnostics and Therapeutic Immunology: Report. 诊断和治疗免疫学研究研讨会:报告。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2015-04-01

Subir Kumar Das

引用次数: 0

Up-regulation of NLRC5 and NF-κB signaling pathway in carrier chickens challenged with Salmonella enterica Serovar Pullorum at different persistence periods. 肠沙门氏菌血清型白痢感染不同持续期载体鸡NLRC5和NF-κB信号通路的上调

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2015-04-01

Xiangping Liu, Teng Ma, Hongzhi Wang, Zhongwei Sheng, Xinhong Dou, Kehua Wang, Zhiteng Li, Zhiming Pan, Guobin Chang, Guohong Chen

{"title":"Up-regulation of NLRC5 and NF-κB signaling pathway in carrier chickens challenged with Salmonella enterica Serovar Pullorum at different persistence periods.","authors":"Xiangping Liu, Teng Ma, Hongzhi Wang, Zhongwei Sheng, Xinhong Dou, Kehua Wang, Zhiteng Li, Zhiming Pan, Guobin Chang, Guohong Chen","doi":"","DOIUrl":"","url":null,"abstract":"The immune performance, SNPs and expression levels of candidate genes (IL1-β, Nramp1, TLR4, MyD88, NF-κB and NLRC5) were analyzed in carrier chickens of a Chinese indigenous breed infected with Salmonella enterica Serovar Pullorum at different persistence periods (12, 19 and 24 weeks of age). Carrier birds at 19 weeks of age presented significant difference in most immune parameters, as compared to carriers at 12 and 24 weeks of age, while no significant difference in most immune parameters was observed between carriers at 12 and 24 weeks of age. The genotype distributions of IL1-β and TLR4 presented significant differences between carriers and healthy birds. The expression levels of most candidate genes in carriers at 19 weeks of age were significantly higher than that in carriers at 12, 24 weeks of age and healthy birds and reached 1% level of significance between carriers at 19 weeks of age and healthy birds. The expression patterns of all genes, but IL-1fl and NLRC5 between carriers at 12 and 24 weeks of age in all tissues were similar. Compared with carriers at 12 weeks of age, IL1-β was significantly down-regulated, but NLRC5 was significantly up-regulated in carriers at 24 weeks of age. Our study demonstrated that immune performance of carrier birds was severely impaired at age of sexual maturation and NLRC5 might play as a negative mediator of NF-κB pathway involved in immune response to asymptomatic infection by S. Pullorum. The TLR4/MyD88/NF-κB pathway might be suitable for study on S. Pullorum infection in Chinese indigenous breeds.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"52 2","pages":"132-9"},"PeriodicalIF":1.4,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33425758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effect of alcoholic extract of stem of Entada pursaetha in dextran sulphate sodium-induced colitis in mice. 马齿苋茎醇提物对硫酸葡聚糖钠致小鼠结肠炎的保护作用。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2015-04-01

Gaurav Gupta, Amar Sunil More, Rashmi Rekha Kumari, Madhu Cholenahalli Lingaraju, Nitya Nand Pathak, Dhirendra Kumar, Dinesh Kumar, Santosh Kumar Mishra, Surender Kumar Tandan

{"title":"Protective effect of alcoholic extract of stem of Entada pursaetha in dextran sulphate sodium-induced colitis in mice.","authors":"Gaurav Gupta, Amar Sunil More, Rashmi Rekha Kumari, Madhu Cholenahalli Lingaraju, Nitya Nand Pathak, Dhirendra Kumar, Dinesh Kumar, Santosh Kumar Mishra, Surender Kumar Tandan","doi":"","DOIUrl":"","url":null,"abstract":"Oxidative stress has been shown to play a critical role in the pathogenesis of ulcerative colitis (UC). Entada pursaetha has been demonstrated to have antioxidant and anti-inflammatory effects. In this study, we investigated the effects of stem of alcoholic extract of E. pursaetha (PSE) in dextran sodium sulfate (DSS)-induced colitis in mice. The protective effect of PSE was determined at three different doses of 30, 100 and 300 mg/kg body weight by oral gavage for 7 days. Morphological (colon length and colon weight/length ratio), clinical (disease activity index) and macroscopic (damage score) features were determined using standard criteria. Lipid peroxides (determined as malonaldehyde; MDA), enzymatic (superoxide dismutase; SOD and catalase; CAT) and non- enzymatic antioxidants (reduced glutathione; GSH), nitrate and nitrite (NOx) levels and myeloperoxidase (PO) activity in colon tissues were determined. The DSS damaged the colonic tissue, increased MPO activity, lipid peroxidation and NOx levels, reduced the antioxidant enzymes and glutathione and lowered the body weight. PSE significantly reduced the inflammation of colon and reversed the increase in MPO activity induced by DSS. It also significantly increased the SOD and catalase activities and did not elicit any effect on depleted levels of GSH in the colonic tissue. In addition, PSE also significantly decreased colonic NOx and MDA levels compared to DSS-treated mice; reduced both infiltration of inflammatory cells and the mucosal damage in colon on histopathological examination. The results suggested the protective potential of PSE in DSS-induced colitis and this might be attributed to its anti-inflammatory and antioxidant activities.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"52 2","pages":"147-54"},"PeriodicalIF":1.4,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33425760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological responses of Catla catla larvae fed with Achyranthes aspera seed enriched diet and exposed to UV-B radiation. 添加牛膝种子饲料和UV-B辐射对Catla Catla幼虫生理反应的影响

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2015-04-01

Jai Gopal Sharma, Moirangthem Kameshwor Singh, Rina Chakrabarti

{"title":"Physiological responses of Catla catla larvae fed with Achyranthes aspera seed enriched diet and exposed to UV-B radiation.","authors":"Jai Gopal Sharma, Moirangthem Kameshwor Singh, Rina Chakrabarti","doi":"","DOIUrl":"","url":null,"abstract":"The antioxidant and growth stimulating properties of seeds of Achyranthes aspera were evaluated on UV-B irradiated Catla catla (catla) larvae. Catla larvae (initial weight: 1.2 ± 0.01 mg) were fed with four different diets--D1, D2 and D3 containing 0.1, 0.25 and 0.5% seeds of A. aspera and D4, control diet for 35 days. The larvae were then exposed to UV-B radiation (80 μW/cm2) on every alternate day for 20 days. Survival, growth, tissue glutamic oxaloacetic transminase (GOT), tissue glutamate pyruvate transminase (GPT), thiobarbituric acid reactive substances (TBARS) and nitric oxide synthase (NOS) were studied in larvae on day-21 of irradiation. Significantly (P < 0.05) higher survival and average weight were found in D3 diet fed fish compared to the other groups. Survival rate was 8-16% higher in seed enriched diet fed groups, compared to the control one. Higher levels of GOT and GPT found in control diet fed larvae showed the degree of tissue damage due to UV-B exposure. Significantly (P < 0.05) lower level of GPT in D3 indicated the UV-B protective effect of the seed of A. Aspera (earlier, the presence of ecdysterone, essential fatty acids and amino acids, polyphenolic compounds, steroids, etc. has been reported from seed). TBARS which indicated the level of tissue lipid peroxidation were significantly (P < 0.05) higher in control group, compared to the other feeding schemes. NOS level was significantly (P < 0.05) higher in D2 and D3, compared to the D1 and control groups. In conclusion, supplementation of A. aspera seed (0.5%) improved the physiological condition (in terms of reduce lipid oxidation and better immune system) and gave bioprotection to catla larvae challenged with UV-B stress.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"52 2","pages":"155-60"},"PeriodicalIF":1.4,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33425761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and response surface optimization of the recovery and purification of recombinant D-galactose dehydrogenase from Pseudomonas fluorescens. 荧光假单胞菌重组d -半乳糖脱氢酶的表达及回收纯化的响应面优化。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2015-02-01

Shadi Rokhsartalab Azar, Raika Naiebi, Ameneh Homami, Zahra Akbari, Anvarsadat Kianmehr, Rahman Mahdizadehdehosta, Faezeh Najafzadeh

{"title":"Expression and response surface optimization of the recovery and purification of recombinant D-galactose dehydrogenase from Pseudomonas fluorescens.","authors":"Shadi Rokhsartalab Azar, Raika Naiebi, Ameneh Homami, Zahra Akbari, Anvarsadat Kianmehr, Rahman Mahdizadehdehosta, Faezeh Najafzadeh","doi":"","DOIUrl":"","url":null,"abstract":"The enzyme D-galactose dehydrogenase (GalDH) has been used in diagnostic kits to screen blood serum of neonates for galactosemia. It is also a significant tool for the measurement of β-D-galactose, α-D-galactose and lactose as well. In this study, response surface methodology (RSM) was used to identify the suitable conditions for recovery of recombinant GalDH from Pseudomonas fluorescens in aqueous two-phase systems (ATPS). The identified GalDH gene was amplified by PCR and confirmed by further cloning and sequencing. E. coli BL-21 (DE3) containing the GalDH gene on a plasmid (pET28aGDH) was used to express and purify the recombinant enzyme. The polyethylene glycol (PEG) and ammonium sulfate concentrations and pH value were selected as variables to analyze purification of GalDH. To build mathematical models, RSM with a central composite design was applied based on the conditions for the highest separation. The recombinant GalDH enzyme was expressed after induction with IPTG. It showed NAD'-dependent dehydrogenase activity towards D-Galactose. According to the RSM modeling, an optimal ATPS was composed of PEG-2000 14.0% (w/w) and ammonium sulfate 12.0% (w/w) at pH 7.5. Under these conditions, GalDH preferentially concentrated in the top PEG-rich phase. The enzyme activity, purification factor (PF) and recovery (R) were 1400 U/ml, 60.0% and 270.0%, respectively. The PEG and salt concentrations were found to have significant effect on the recovery of enzyme. Briefly, our data showed that RSM could be an appropriate tool to define the best ATPS for recombinant P. fluorescens GalDH recovery.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"52 1","pages":"68-74"},"PeriodicalIF":1.4,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33356572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induced defence responses of contrasting bread wheat genotypes under differential salt stress imposition. 不同盐胁迫下不同面包小麦基因型的诱导防御反应。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2015-02-01

Archana Singh, Bharat Bhushan, Kishor Gaikwad, O P Yadav, Suresh Kumar, R D Rai

{"title":"Induced defence responses of contrasting bread wheat genotypes under differential salt stress imposition.","authors":"Archana Singh, Bharat Bhushan, Kishor Gaikwad, O P Yadav, Suresh Kumar, R D Rai","doi":"","DOIUrl":"","url":null,"abstract":"Plants, being sessile in nature, have developed mechanisms to cope with high salt concentrations in the soil. In this study, the effects of NaCl (50-200 mM) on expression of high-affinity potassium transporters (HKTs), antioxidant enzymes and their isozyme profiles were investigated in two contrasting bread wheat (Triticum aestivum L.) genotypes viz., HD2329 (salt-sensitive) and Kharchia65 (salt-tolerant). Kharchia65 can successfully grow in salt affected soils, while HD2329 cannot tolerate salt stress. Differential expression studies of two HKT genes (TaHKT2;1.1 and TaHKT2;3.1) revealed their up-regulated expression (-1.5-fold) in the salt-sensitive HD2329 and down-regulated (-5-fold) inducible expression in the salt-tolerant genotype (Kharchia65). Specific activity of antioxidant enzymes, viz. superoxide dismutase (SOD), peroxidase (POX), ascorbate peroxidase (APX), catalase (CAT) and glutathione reductase (GR) was found to be higher in the salt-tolerant genotype. Isozyme profile of two (POX and GR) antioxidant enzymes showed polymorphism between salt-tolerant and salt-sensitive genotypes. A new gene TaHKT2;3.1 was also identified and its expression profile and role in salt stress tolerance in wheat was also studied. Partial sequences of the TaHKT2;1.1 and TaHKT2;3.1 genes from bread wheat were submitted to the EMBL GenBank database. Our findings indicated that defence responses to salt stress were induced differentially in contrasting bread wheat genotypes which provide evidences for functional correlation between salt stress tolerance and differential biochemical and molecular expression patterns in bread wheat.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"52 1","pages":"75-85"},"PeriodicalIF":1.4,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33356573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analyzing resistance pattern of non-small cell lung cancer to crizotinib using molecular dynamic approaches. 应用分子动力学方法分析非小细胞肺癌对克唑替尼的耐药模式。

IF 1.4 4区生物学

Indian journal of biochemistry & biophysics Pub Date : 2015-02-01

Anish Kumar, K Ramanathan

引用次数: 0