{"title":"The Improving anti-tumour activity with melatonin-stimulated mesenchymal stem cell-derived exosomes in metastatic triple-negative breast cancer","authors":"","doi":"10.56042/ijbb.v60i11.1291","DOIUrl":null,"url":null,"abstract":"The aim of this study is to increase the bioavailability of melatonin on triple-negative breast cancer (TNBC) cells by loading it into exosomes as well as comparing the therapeutic potentials of melatonin and exosome released from human adipose tissue-derived mesenchymal stem cells. TNBC is one of the most malignant tumours with highly invasive and metastatic features. It is characterized by the absence of estrogen receptor, progesterone receptor and human epidermal growth factor receptor 2. TNBC patients can’t benefit from hormonal or trastuzumab-based therapies targeting these receptors. Exosomes are defined as naturally occurring extracellular vesicles. By enabling the transfer of molecules, exosomes play a role in cancer treatment and dynamic intercellular communication between tumour cells and adjacent stromal compartments. The half-maximum inhibitory concentration IC50 values were 30.38 µg/mL, 40.49 µg/mL and 1.5 mM at the co-administered melatonin and exosome, AT-Exo and Mel groups, respectively, for 48 h. The percentage of late-stage apoptotic induction was found to be 6.3%, 4.1% and 4.6% for TNBC exposed to co-administered melatonin and exosome (2.5 mM +100 µg/mL Mel/Exo) for 24 h, 48 h, 72 h, respectively. In conclusion, the coexistence of exosomes and melatonin represents a promising therapeutic tool that can interfere with key molecular processes such as cytotoxicity and apoptosis cascade in TNBC.","PeriodicalId":13281,"journal":{"name":"Indian journal of biochemistry & biophysics","volume":"29 1","pages":"0"},"PeriodicalIF":1.5000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian journal of biochemistry & biophysics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.56042/ijbb.v60i11.1291","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The aim of this study is to increase the bioavailability of melatonin on triple-negative breast cancer (TNBC) cells by loading it into exosomes as well as comparing the therapeutic potentials of melatonin and exosome released from human adipose tissue-derived mesenchymal stem cells. TNBC is one of the most malignant tumours with highly invasive and metastatic features. It is characterized by the absence of estrogen receptor, progesterone receptor and human epidermal growth factor receptor 2. TNBC patients can’t benefit from hormonal or trastuzumab-based therapies targeting these receptors. Exosomes are defined as naturally occurring extracellular vesicles. By enabling the transfer of molecules, exosomes play a role in cancer treatment and dynamic intercellular communication between tumour cells and adjacent stromal compartments. The half-maximum inhibitory concentration IC50 values were 30.38 µg/mL, 40.49 µg/mL and 1.5 mM at the co-administered melatonin and exosome, AT-Exo and Mel groups, respectively, for 48 h. The percentage of late-stage apoptotic induction was found to be 6.3%, 4.1% and 4.6% for TNBC exposed to co-administered melatonin and exosome (2.5 mM +100 µg/mL Mel/Exo) for 24 h, 48 h, 72 h, respectively. In conclusion, the coexistence of exosomes and melatonin represents a promising therapeutic tool that can interfere with key molecular processes such as cytotoxicity and apoptosis cascade in TNBC.
期刊介绍:
Started in 1964, this journal publishes original research articles in the following areas: structure-function relationships of biomolecules; biomolecular recognition, protein-protein and protein-DNA interactions; gene-cloning, genetic engineering, genome analysis, gene targeting, gene expression, vectors, gene therapy; drug targeting, drug design; molecular basis of genetic diseases; conformational studies, computer simulation, novel DNA structures and their biological implications, protein folding; enzymes structure, catalytic mechanisms, regulation; membrane biochemistry, transport, ion channels, signal transduction, cell-cell communication, glycobiology; receptors, antigen-antibody binding, neurochemistry, ageing, apoptosis, cell cycle control; hormones, growth factors; oncogenes, host-virus interactions, viral assembly and structure; intermediary metabolism, molecular basis of disease processes, vitamins, coenzymes, carrier proteins, toxicology; plant and microbial biochemistry; surface forces, micelles and microemulsions, colloids, electrical phenomena, etc. in biological systems. Solicited peer reviewed articles on contemporary Themes and Methods in Biochemistry and Biophysics form an important feature of IJBB.
Review articles on a current topic in the above fields are also considered. They must dwell more on research work done during the last couple of years in the field and authors should integrate their own work with that of others with acumen and authenticity, mere compilation of references by a third party is discouraged. While IJBB strongly promotes innovative novel research works for publication as full length papers, it also considers research data emanating from limited objectives, and extension of ongoing experimental works as ‘Notes’. IJBB follows “Double Blind Review process” where author names, affiliations and other correspondence details are removed to ensure fare evaluation. At the same time, reviewer names are not disclosed to authors.