Indian Journal of Clinical Biochemistry最新文献

{"title":"Decoding the Link: Exploring FMO3 Gene Variations as a Key to Understanding Type Two Diabetes Through Bioinformatics and Case-Control Analyses.","authors":"Shahrzad Shokati, Saman Sargazi, Mahdi Majidpour, Shekoufeh Mirinejad, Zari Naderi Ghale-Noie, Mansour Shahraki, Sara Sargazi, Maryam Piri","doi":"10.1007/s12291-024-01295-9","DOIUrl":"10.1007/s12291-024-01295-9","url":null,"abstract":"<p><p>This study is aimed to examine the association between three variants of the <i>FMO3</i> gene-rs2266782G/A (E158K), rs2266780A/G (E308G), and rs1736557G/A (V257M)-and susceptibility to type 2 diabetes mellitus (T2DM), given the role of flavin-containing dimethylaniline monooxygenases (FMOs) in catalyzing trimethylamine N-oxide (TMAO), a compound linked to T2DM. A total of 800 adults were genotyped using PCR-RFLP or ARMS-PCR techniques, and various metabolic parameters, including lipid profiles and fasting blood sugar levels, were recorded for each participant. The findings revealed a significant association between the rs1736557 variant and T2DM under multiple inheritance models. Notably, the A allele of rs1736557 and the G allele of rs2266780 were associated with a decreased risk of T2DM, with risk reductions of 38% and 25%, respectively. Some genotype combinations exhibited a negative correlation with T2DM susceptibility, while certain haplotypes correlated with an increased risk of the disease. Bioinformatics analyses indicated that these genetic variants did not alter the secondary structure of FMO3 mRNA. Ultimately, the study identified significant associations between specific <i>FMO3</i> variations and T2DM risk, emphasizing the need for further research with diverse populations to validate these findings.</p><p><strong>Graphical abstract: </strong></p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at https://doi.org/10.1007/s12291-024-01295-9 .</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"41 5","pages":"721-735"},"PeriodicalIF":1.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13550392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148911632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Anticancer Efficacy of Selenium Nanoparticles Encapsulated in Niosomes: A Novel Therapeutic Strategy.","authors":"Faezeh Amiri, Farhad Alishahi, Golnoush Mohammadifar, Shadi Izadidehkordi, Forugh Charmduzi, Fatemeh Dialameh, Azim Akbarzadeh Khiyavi","doi":"10.1007/s12291-025-01321-4","DOIUrl":"10.1007/s12291-025-01321-4","url":null,"abstract":"<p><p>Oral squamous cell carcinoma persists as a formidable global health challenge, characterized by notably high morbidity and mortality rates. In this study, the potential of selenium nanoparticles, meticulously encapsulated within niosomes, is explored as a novel therapeutic strategy against HSC-3-a cell line that serves as a model for oral cancer. Employing a thin-film hydration technique for the synthesis of selenium nanoparticles, the resultant nanoparticle formulations were, through the application of dynamic light scattering (DLS), characterized with respect to particle size, zeta potential, and polydispersity index (PDI). The anticancer efficacy of this formulation was evaluated through the MTT assay at time intervals of 24, 48, and 72 h to rigorously assess the cytotoxic effects on HSC-3 cells. Notably, the niosome-encapsulated selenium nanoparticles exhibited a mean diameter of 220 nm and a zeta potential of - 30 mV, indicative of commendable stability, while the consistently low PDI (below 0.2) underscored a uniform particle size distribution. In vitro cytotoxicity results compellingly revealed that these selenium nanoparticles substantially reduced the viability of HSC-3 cells in a manner that was dependent on the duration of exposure, with the most pronounced reduction observed at 72 h post-treatment. Collectively, these findings emphasize the improved anticancer efficacy resulting from the niosomal encapsulation of selenium nanoparticles, which induces significantly enhanced cytotoxic effects against HSC-3 cells. This suggests that niosome-encapsulated selenium nanoparticles may offer considerable promise as a novel therapeutic strategy for the treatment of oral squamous cell carcinoma.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"41 5","pages":"786-791"},"PeriodicalIF":1.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13550371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148911649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coumarins at the Crossroads of Drug Development: Enzyme Modulation for Clinical Advances.","authors":"Yasser Fakri Mustafa","doi":"10.1007/s12291-025-01331-2","DOIUrl":"10.1007/s12291-025-01331-2","url":null,"abstract":"<p><p>Coumarins, a diverse class of naturally occurring and synthetic compounds, have garnered significant attention in drug development due to their ability to modulate enzymatic activity. These molecules exhibit dual functionality, acting as both enzyme inhibitors and enzyme inducers, influencing various biochemical pathways. Their structural flexibility allows for diverse interactions with key enzymes, making them valuable in pharmacology, particularly in the treatment of cancer, cardiovascular diseases, and neurodegenerative disorders. This comprehensive review explores the intricate mechanisms through which coumarins interact with enzymes. Also, it discusses the role of these compounds in competitive, non-competitive, and mixed inhibition, where they interfere with enzymatic processes by either occupying active sites or altering enzyme conformation. Conversely, certain coumarins function as enzyme inducers, enhancing enzymatic activity through transcriptional upregulation and post-translational modifications. These properties position coumarins as promising therapeutic agents, offering both inhibitory and activating effects within the same biochemical pathways. Furthermore, this work examines how these interactions influence drug metabolism, particularly through cytochrome P450 enzymes and UDP-glucuronosyltransferases, which are critical in drug biotransformation and detoxification. The pharmacokinetic and pharmacodynamic profiles of coumarins, including their bioavailability, metabolism, and therapeutic efficacy, are also analyzed to provide insights into optimizing their clinical applications. By bridging fundamental biochemical insights with clinical potential, this in-depth review underscores the necessity of further research to refine coumarin-based therapies. Future investigations should focus on structural modifications, targeted drug delivery systems, and personalized medicine approaches to maximize their therapeutic benefits while minimizing adverse effects. As the biochemical understanding of coumarins deepens, their role in precision medicine and enzyme-targeted therapies is poised to expand, unlocking new possibilities in disease treatment and drug design.</p><p><strong>Graphical abstract: </strong></p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"41 5","pages":"683-706"},"PeriodicalIF":1.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13550334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148911661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Reference Interval Values of Biochemical Parameters in Iranian Kurdish Adults: Results from Non-communicable Diseases of Ravansar Cohort.","authors":"Maryam Kohsari, Zohreh Rahimi, Farid Najafi, Mehdi Moradinazar","doi":"10.1007/s12291-024-01286-w","DOIUrl":"10.1007/s12291-024-01286-w","url":null,"abstract":"<p><p>This study aimed to establish age- and sex-specific biochemical parameters Reference intervals (RIs) in a healthy adult Kurdish population. The biochemical markers were investigated in 2850 individuals (1365 female and 1485 male) from Ravansar Non-Communicable Disease (RaNCD) cohort study. The biochemical markers were measured using an enzymatic assay. The non-parametric method was used to calculate RIs for all partitions. The liver enzymes and TG, HDL-C, and creatinine levels were gender- and age-dependent. The RIs of these parameters in the Iranian population are dramatically lower compared to other populations. Smokers and overweight subjects had higher liver enzymes and lipid profile levels. Similar results were obtained in post-menopause females. Individuals in the high physical activity group had a lower activity of ALP and GGT and a higher ALT and AST, also, lower lipid profile (TC, TG, and LDL) and FBS, and higher HDL levels. Our study suggests that chemical biomarkers are influenced by gender and age, and factors such as BMI, smoking status, physical activity, and menopause status must be considered in laboratory analysis. Additionally, the results indicated that reference values of commercial kits routinely used, especially liver enzymes, could not be appropriate for the Iranian Kurdish population.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"41 5","pages":"736-745"},"PeriodicalIF":1.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13550255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148911730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HDL or hs-CRP: The Prognostic Conundrum in Acute Coronary Syndrome.","authors":"Siddharth Gosavi, Mounica Chekuri, M Mukhyaprana Prabhu, Padmakumar Ramachandran, Bharath R Kumar, Renuka Suvarna, Monalisa Biswas","doi":"10.1007/s12291-024-01282-0","DOIUrl":"10.1007/s12291-024-01282-0","url":null,"abstract":"<p><p>There is a dearth of information from research regarding the correlation between high density lipoprotein (HDL) and high-sensitivity C-reactive protein (hs-CRP) and acute coronary syndrome (ACS). The objective of our research was to look into their relationship as well as their impact on the hospital mortality and clinical outcomes of ACS patients. Patient of either gender of age > 18 years with new onset of ACS confirmed by clinical presentation, ECG changes and levels of cardiac necrosis biomarkers were included in the study. Complete blood count, HDL, hs-CRP and albumin investigations were done. Based on their HDL cholesterol levels, patients were categorized into three groups: very low HDL, low HDL, and normal HDL. The results demonstrated a strong and statistically significant correlation between hs-CRP and HDL (<i>p</i> < 0.05). No relationship was observed between low HDL and total counts in patients with ACS. Correlation of inflammatory markers with HDL level showed that hs-CRP is highly corelated with HDL (<i>r</i> = - 0.419, <i>p</i> < 0.001), whereas albumin and WBC was not statistically significant. Although low HDL does not directly correlate with the severity of the disease, low HDL-c is a risk factor for coronary heart disease. Elevated hs-CRP levels in ACS patients are linked to heart failure, severe left ventricular dysfunction, and a higher risk of hospital complications. As a result, hs-CRP can be regarded as a relatively simple biomarker that aids in determining the patient's spectrum with ACS.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"41 5","pages":"765-772"},"PeriodicalIF":1.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13550344/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148911732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Variations in TLR7 and Their Role in Systemic Lupus Erythematosus: A Systematic Review and Meta-analysis.","authors":"Lisie L Patnayak, Seema Shah, Suprava Patel, Rachita Nanda, Eli Mohapatra","doi":"10.1007/s12291-024-01294-w","DOIUrl":"10.1007/s12291-024-01294-w","url":null,"abstract":"<p><p>Systemic Lupus Erythematosus (SLE) is a complex autoimmune disease with multi-systemic involvement. It is characterized by the excessive production of auto-antibodies and immune complex deposition which occurs due to improper functioning of TLR7 (toll-like receptor 7). This systematic review and meta-analysis aims to study the genetic polymorphism in TLR7 and its risk to the development of SLE. PubMed, Scopus and Cochrane central were searched for articles published till August 2023 that examined TLR7 polymorphism in SLE. Case-control, cohort, and cross-sectional studies reporting clear data on genotype frequency were selected. Odds Ratio (OR) and 95% confidence interval was calculated for each study, and publication risk was calculated using Egger's test. Although we started with 8 (single nucleotide polymorphism) SNPs for systematic review, meta-analysis was done on 3 SNPs based on the inclusion criteria. A total of 10 studies involving 10,917 cases and 10,945 controls for rs3853839, rs179008, and rs179010 were included in our meta-analysis. The pooled analysis showed no association of TLR7 gene polymorphism and SLE susceptibility for rs3853839, rs179008, and rs179010 in all models including allelic, homozygous, heterozygotes, dominant, and recessive models. Notably no heterogeneity was reported for rs179008. In contrast allelic, homozygous, dominant and recessive models of rs3853839 showed significant heterogeneity of <i>p</i> < 0.05. Overall, our meta-analysis suggests that these TLR7 genetic variants do not influence the development of SLE.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"41 5","pages":"707-720"},"PeriodicalIF":1.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13550256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148911751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum 17 Alpha-Hydroxyprogesterone Analysis- Performance Evaluation of Maglumi^® X-8 Chemiluminescence Immunoassay.","authors":"Sibtain Ahmed, Ayra Siddiqui, Shan Lakhani, Samia Khan, Rizwana Kausar, Imran Siddiqui","doi":"10.1007/s12291-024-01277-x","DOIUrl":"10.1007/s12291-024-01277-x","url":null,"abstract":"<p><p>Congenital Adrenal Hyperplasia (CAH) is primarily caused by 21-hydroxylase deficiency, leading to abnormal adrenal hormone production. Accurate measurement of 17-hydroxyprogesterone (17-OHP) is critical for diagnosing and managing CAH. Traditionally, 17-OHP levels are assessed using ELISA, but methods like chemiluminescent immunoassays (CLIA) offer potential improvements in accuracy and efficiency. We evaluated the Maglumi^® X-8 CLIA system for measuring 17-OHP levels and compared its performance with the established ELISA method. The study included serum samples from 39 patients, analyzed for accuracy, precision, linearity, and reportable range. The correlation between CLIA and ELISA results was assessed using regression analysis and Bland-Altman plots. The Maglumi^® X-8 CLIA demonstrated acceptable precision, with low coefficients of variation (CVs) at both low and high 17-OHP concentrations. The assay showed strong correlation with the ELISA method (slope: 0.961, intercept: 1.712) and consistent accuracy across a broad reportable range (0.10 to 320 ng/mL). The CLIA system also proved to be faster and more automated, reducing the potential for human error and facilitates faster results for clinical decision making. The Maglumi^® X-8 CLIA is a highly precise, accurate, and efficient method for measuring 17-OHP levels, making it a suitable alternative to ELISA in clinical laboratories. Its integration into routine workflows offers faster results and improved reliability.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"41 5","pages":"799-804"},"PeriodicalIF":1.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13550317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148911801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights for miR-9, miR-21, and miR-34a Expression Levels and Their Cross-Talk with TNF-α, IL-1β, and Nrf2 Levels in Diabetic Nephropathy Patients.","authors":"Rana Adel, Adel Abdel-Moneim, Ahmed I Yousef, Amr E Ahmed, Alaa M Rabea, Nabil A Hasona","doi":"10.1007/s12291-024-01288-8","DOIUrl":"10.1007/s12291-024-01288-8","url":null,"abstract":"<p><p>Oxidative stress and inflammatory mediators are considered the main culprits responsible for orchestrating diabetic complications and triggering the onset of diabetes mellitus. Members of the noncoding ribonucleic acid (ncRNAs) play critical roles in regulating various diabetic complications. However, the role of the micro-ncRNAs (miR-9, miR-21, and miR-34a) in diabetic nephropathy remains elusive. Hence, we determined the prospective value of serum miR-9, miR-21, and miR-34a as biological indicators for diabetic nephropathy and examined their correlations with TNF-α, IL-1β, and Nrf2 signaling in patients with DN. This study involved healthy individuals represented by the control group and individuals with DM classified into non-nephropathy group and diabetic nephropathy group. Those with DM exhibited noticeable increases in parameters related to diabetes as fasting blood sugar, and HbA1c. Furthermore, the diabetic groups had substantially significant elevated levels of TNF-α compared to the control healthy group. Interestingly, the molecular biomarkers miR-9, miR-21, and miR-34a expressions were significantly higher in diabetic patients who showed interrupted glomerular filtration rate (eGFR) function. On the other hand, the expression of circulating Nfr2 in blood exhibited a sustained low level of expression in DM groups exhibiting a negative correlation with miR-9, miR-21, miR-34a, TNF-α and IL1β levels. This study highlights the potential of non-coding RNA expression as a prospective biomarker for diabetes mellitus. These biomarkers could potentially assist in predicting the progression of associated complications.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"41 5","pages":"773-779"},"PeriodicalIF":1.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13550352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148911716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Notification to Clinical Disposition: Closing the Loop for Critical Laboratory Results.","authors":"Prasenjit Mitra, Praveen Sharma","doi":"10.1007/s12291-026-01453-1","DOIUrl":"10.1007/s12291-026-01453-1","url":null,"abstract":"","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"41 5","pages":"645-647"},"PeriodicalIF":1.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13550252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148911719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling Role of Serum Total Bile Acid in Diabetic Osteopathy, the Unexplored Complication of Diabetes.","authors":"Mantu Jain, Chhabi Rani Panda, Paulson Varghese, Saurav Nayak, Sujit Tripathy, Gautom Kumar Saharia, Manaswini Mangaraj","doi":"10.1007/s12291-024-01289-7","DOIUrl":"10.1007/s12291-024-01289-7","url":null,"abstract":"<p><p>As a signaling molecule and modulator of glucose and lipid metabolism, the role played by bile acid in regulating bone health in Type 2 Diabetes Mellitus needs evaluation on the basis of previous observations of interrelation of diabetes with osteoporosis and association of bile acid with bone metabolism. 46 Type 2 diabetics and 46 non-diabetic control admitted to orthopedic IPD for surgical treatment were included in the study. They were evaluated for Lumbar Bone mineral density (BMD), serum total bile acid, bone turnover markers PIPN and CTX, along with vitamin D, serum calcium and phosphate after submission of written consent. Association of total bile acid with glycemic status and bone tumor marker was assessed by regression analysis. Correlation of bile acid with BMD, CTX, and P1PN was assessed by spearman correlation test using IBM SPSS software (26 version). Diabetics revealed significantly low BMD and low bone turnover markers with elevated serum total bile acid. Bile acid registered a significant positive association with glycemic parameters and marked negative relation with BMD and bone tumor markers. Persistent relation was also noticed with CTX and vitamin D even after confounded with glycemic status. As a metabolic regulator, Bile acid exhibited an impact on bone health in Type 2 diabetics increasing the risk for fragility fracture. Detailed metabolic profile evaluation as well as its integrated role with gut microbiota, needs to be explored for more insight into its molecular mechanism and future therapeutic implications.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"41 5","pages":"780-785"},"PeriodicalIF":1.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13550263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148911779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}