{"title":"Plasma-Derived Exosomal tRF-Phe-GAA-001 and tRF-Gly-GCC-037 as Novel Diagnostic Biomarkers for Cervical Cancer.","authors":"Zheng Li, Hongyan Wang, Ruijun Yang, Xiangchun Jin, Qing Han, Zhaoyuan She, Peng Ge","doi":"10.1007/s12291-024-01235-7","DOIUrl":null,"url":null,"abstract":"<p><p>This study delves into the exploration of exosomal transfer RNA-derived fragments (tRFs) as potential diagnostic markers for cervical cancer (CC). Employing plasma-derived exosomes isolated through ultracentrifugation and confirmed via transmission electron microscopy (TEM), qNano, and western blot analysis, we extracted total RNA from CC and adjacent tissues (n = 48), alongside exosomes from cervical cancer patients (n = 140) and healthy donors (n = 140) using Trizol reagents. The expression of exosomal tRFs was assessed through quantitative polymerase chain reaction (qPCR) and subjected to statistical analysis using Mann-Whitney U or t-tests, along with receiver operating characteristic (ROC) analysis. The findings unveiled a significant downregulation of exosomal tRF-Phe-GAA-001 and tRF-Gly-GCC-037 in both CC tissues and plasma samples from early-stage patients compared to healthy controls. Remarkably, these two exosomal tRFs exhibited promising capabilities as circulating biomarkers for both the diagnosis and early detection of CC, as evidenced by their high area under the curve (AUC) values of 0.9337 and 0.9432, respectively. Consequently, exosomal tRF-Phe-GAA-001 and tRF-Gly-GCC-037 were downregulated in CC and early-stage CC, indicating their potential as innovative non-invasive biomarkers for early CC diagnosis.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12291-024-01235-7.</p>","PeriodicalId":13280,"journal":{"name":"Indian Journal of Clinical Biochemistry","volume":"40 4","pages":"683-690"},"PeriodicalIF":1.6000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12420557/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Clinical Biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s12291-024-01235-7","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/25 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
This study delves into the exploration of exosomal transfer RNA-derived fragments (tRFs) as potential diagnostic markers for cervical cancer (CC). Employing plasma-derived exosomes isolated through ultracentrifugation and confirmed via transmission electron microscopy (TEM), qNano, and western blot analysis, we extracted total RNA from CC and adjacent tissues (n = 48), alongside exosomes from cervical cancer patients (n = 140) and healthy donors (n = 140) using Trizol reagents. The expression of exosomal tRFs was assessed through quantitative polymerase chain reaction (qPCR) and subjected to statistical analysis using Mann-Whitney U or t-tests, along with receiver operating characteristic (ROC) analysis. The findings unveiled a significant downregulation of exosomal tRF-Phe-GAA-001 and tRF-Gly-GCC-037 in both CC tissues and plasma samples from early-stage patients compared to healthy controls. Remarkably, these two exosomal tRFs exhibited promising capabilities as circulating biomarkers for both the diagnosis and early detection of CC, as evidenced by their high area under the curve (AUC) values of 0.9337 and 0.9432, respectively. Consequently, exosomal tRF-Phe-GAA-001 and tRF-Gly-GCC-037 were downregulated in CC and early-stage CC, indicating their potential as innovative non-invasive biomarkers for early CC diagnosis.
Supplementary information: The online version contains supplementary material available at 10.1007/s12291-024-01235-7.
期刊介绍:
The primary mission of the journal is to promote improvement in the health and well-being of community through the development and practice of clinical biochemistry and dissemination of knowledge and recent advances in this discipline among professionals, diagnostics industry, government and non-government organizations. Indian Journal of Clinical Biochemistry (IJCB) publishes peer reviewed articles that contribute to the existing knowledge in all fields of Clinical biochemistry, either experimental or theoretical, particularly deal with the applications of biochemistry, molecular biology, genetics, biotechnology, and immunology to the diagnosis, treatment, monitoring and prevention of human diseases. The articles published also include those covering the analytical and molecular diagnostic techniques, instrumentation, data processing, quality assurance and accreditation aspects of the clinical investigations in which chemistry has played a major role, or laboratory animal studies with biochemical and clinical relevance.