Immunochemistry最新文献_第9页

Viral infections—A clinical approach 病毒感染——临床方法

Immunochemistry Pub Date : 1978-06-01 DOI: 10.1016/0161-5890(78)90140-2

Julius S. Horoszewicz

引用次数: 0

Isolation and characterization of a human mononuclear cell Fc receptor 人单核细胞Fc受体的分离与鉴定

Immunochemistry Pub Date : 1978-06-01 DOI: 10.1016/0161-5890(78)90132-3

Charlotte Cunningham-Rundles, Frederick P. Siegal, Robert A. Good

引用次数: 25

Characterization of antibodies against ceramidetrihexoside and globoside 神经酰胺三己糖苷和球苷抗体的鉴定

Immunochemistry Pub Date : 1978-06-01 DOI: 10.1016/0161-5890(78)90130-X

Mic.N. Hamers , Wilma E. Donker-Koopman , Marie-Jose`phe Coulon-Morelec , Pierre Dupouey , Joseph M. Tager

{"title":"Characterization of antibodies against ceramidetrihexoside and globoside","authors":"Mic.N. Hamers , Wilma E. Donker-Koopman , Marie-Jose`phe Coulon-Morelec , Pierre Dupouey , Joseph M. Tager","doi":"10.1016/0161-5890(78)90130-X","DOIUrl":"10.1016/0161-5890(78)90130-X","url":null,"abstract":"<div>1. When a carrier-hapten complex was used to raise antibodies in rabbits against ceramidetrihexoside, an antiserum was obtained in which the activity against the carrier could not be removed by absorption with the carrier without loss of the anti-hapten activity.2. Antibodies against ceramidetrihexoside were raised in rabbits by immunizing them with a mixture of bovine serum albumin and the glycolipid according to Hakomori (1972). It was found that these antibodies cross-reacted with digalactosylceramide. but not with lactosylceramide or digalactosyldiglyceride. No cross-reactivity was observed with globoside in an artificial membrane system but a slight cross-reactivity was found with globoside absorbed on cholesterol microcrystals. A possible explanation for the latter phenomenon is discussed. From inhibition studies with different sugar compounds combined with the cross-reactivity studies, it can be concluded that anti-ceramidetrihexoside antibodies have a high and strict specificity for a terminal galactosyl α(1→ 4)galactosyl group.3. Antibodies raised in rabbits against globoside by the method of Hakomori (1972) did not cross-react with the other glycolipids tested. The characteristics of these antibodies are compared with those of the anti-ceramidetrihexoside antibodies.4. A human anti-P1,P,Pk serum from a person with the rare phenotype p, which contains anticeramidetrihexoside (anti-Pk) activity was compared with the rabbit anti-ceramidetrihexoside serum. The two antisera showed very similar characteristics with regard to the anti-ceramidetrihexoside activity.</div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":"15 6","pages":"Pages 353-358"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90130-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11900789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Antigenicity of the phosphatidyl inositomannosides of mycobacteria 分枝杆菌磷脂酰肌醇苷的抗原性

Immunochemistry Pub Date : 1978-06-01 DOI: 10.1016/0161-5890(78)90131-1

B. Banerjee, D. Subrahmanyam

引用次数: 6

Viral infections—A clinical approach 病毒感染——临床方法

Immunochemistry Pub Date : 1978-06-01 DOI: 10.1016/0161-5890(78)90140-2

J. Horoszewicz

引用次数: 0

Identification and purification of an organ specific, tumor membrane associated antigen from a spontaneously metastasizing rat mammary carcinoma 从自发性转移的大鼠乳腺癌中鉴定和纯化器官特异性肿瘤膜相关抗原

Immunochemistry Pub Date : 1978-06-01 DOI: 10.1016/0161-5890(78)90129-3

S.K. Ghosh, A.L. Grossberg, U. Kim, D. Pressman

{"title":"Identification and purification of an organ specific, tumor membrane associated antigen from a spontaneously metastasizing rat mammary carcinoma","authors":"S.K. Ghosh, A.L. Grossberg, U. Kim, D. Pressman","doi":"10.1016/0161-5890(78)90129-3","DOIUrl":"10.1016/0161-5890(78)90129-3","url":null,"abstract":"<div>An organ specific (common histotypic) antigen has been identified and partially purified from a spontaneously metastasizing rat mammary carcinoma, TMT-081. The antigen is present in the tumor at a level about 50–200 times greater than in non-malignant, hyperplastic mammary tissue.The antigen is a membrane associated glycoprotein and was solubilized by limited papain digestion of tumor cell membranes or by extraction with non-ionic detergent. Partial purification of papain solubilized antigen by gel filtration and lectin affinity chromatography yielded material that was 50% precipitable by a specific rabbit antibody reagent obtained from antiserum raised against the tumor. The apparent molecular weight of the partially purified antigen was about 28.000 as determined by SDS-PAGE. Quantitation of the antigen was accomplished by a sensitive binding inhibition radioimmune assay. The antigen was present in hyperplastic mammary tissue and was not detected in other normal adult or fetal rat tissues and in a polyoma virus induced syngeneic rat fibrosarcoma. The antigen could be detected in the serum of tumor bearing animals at concentrations reflecting the tumor mass present.</div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":"15 6","pages":"Pages 345-352"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90129-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11319755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Genetic control of the content and avidity of hapten-binding B lymphocytes in mouse spleen 小鼠脾脏半抗原结合B淋巴细胞含量和活性的遗传调控

Immunochemistry Pub Date : 1978-06-01 DOI: 10.1016/0161-5890(78)90134-7

B.B. Fuchs, J.V. Spirande

{"title":"Genetic control of the content and avidity of hapten-binding B lymphocytes in mouse spleen","authors":"B.B. Fuchs, J.V. Spirande","doi":"10.1016/0161-5890(78)90134-7","DOIUrl":"10.1016/0161-5890(78)90134-7","url":null,"abstract":"<div>The content and the avidity curves of IgM-positive spleen B lymphocytes forming rosettes with TNP-SRBCI were compared in C57BL/6 and BALB/c mice (C57BL/6 × BALB/c)F1 hybrids, as well as F1 × C57BL/6 and F1 × BALB/c backcross hybrids. Conjugates of bovine serum albumin with trinitrophenyl. dinitrophenylsulfonic and sulfanilic acids were used as inhibitors (TNP24BSA, DNP23BSA, Sulf17BSA).The spleen TNP-RFC content in BALB/c mice was 60% higher than in the C57BL/6 strain. F1 hybrids were intermediate in this respect. F1 × BALB/c hybrids had on the average 35% more TNP/RFC than F1 × C57BL/6 mice.The TNP-RFC inhibition (avidity) curves obtained with TNP24BSA, DNP23BSA and Sulf17BSA were markedly different in BALB/c, C57BL/6 and (BALB/c × C57BL/6)F1 animals. We conclude that the spleen content and avidity of TNP-RFC in mice are subject to strict genetic control.Assuming random expression of V (idiotype?) genes, the present results seem to indicate that the content and proportions of groups of B clones are controlled by structural immunoglobulin genes. The simplest control mechanism could be provided by definite quantitative relationships between corresponding groups of V genes.</div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":"15 6","pages":"Pages 379-383"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90134-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11521938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid purification of detergent-solubilized Ia antigens by immunoabsorbent chromatography 用免疫吸收层析法快速纯化洗涤剂溶解型Ia抗原

Immunochemistry Pub Date : 1978-06-01 DOI: 10.1016/0161-5890(78)90137-2

Loran T. Clement, Anne M. Kask, Ethan M. Shevach

引用次数: 8

Purification of the i-antigen 51A fromParamecium tetraurelia by immunoaffinity chromatography 免疫亲和层析法纯化四甲草履虫i抗原51A

Immunochemistry Pub Date : 1978-06-01 DOI: 10.1016/0161-5890(78)90133-5

Richard H. Davis Jr., Edward Steers Jr.

{"title":"Purification of the i-antigen 51A fromParamecium tetraurelia by immunoaffinity chromatography","authors":"Richard H. Davis Jr., Edward Steers Jr.","doi":"10.1016/0161-5890(78)90133-5","DOIUrl":"10.1016/0161-5890(78)90133-5","url":null,"abstract":"<div>Purification of the surface antigen ofParamecium tetraurelia (termed i-antigen) by previously published procedures has been shown to result in preparations which frequently contain contaminating proteins including a thiol-activated protease. Alternate procedures for the purification of i-antigen were developed using ion-exchange chromatography. While certain of these procedures result in a homogeneous preparation of i-antigen, the poor yields obtained make these procedures impractical. An alternate method for the purification of i-antigen was developed using immunoaffinity chromatography. Purification by this technique proved to be superior to the various standard procedures described previously. The immunoaffinity column is rapid and results in a recovery, on the average, of over 95% of the applied material. The columns are readily regenerated and may be used numerous times without loss of capacity or purification ability. The i-antigen purified by this method appears homogeneous immunologically and electrophoretically on polyacrylamide gel electrophoresis and isoelectric focusing.</div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":"15 6","pages":"Pages 371-378"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90133-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11900794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Evidences proving the intercalation hypothesis of the C-mediated complex release activity (CRA) c介导的复合物释放活性(CRA)嵌入假说的证据

Immunochemistry Pub Date : 1978-05-01 DOI: 10.1016/0161-5890(78)90095-0

E´va Rajnavo¨lgyi , G. Fu¨st , Julia Ember , G.A. Medgyesi , J. Gergely

引用次数: 4