{"title":"小鼠脾脏半抗原结合B淋巴细胞含量和活性的遗传调控","authors":"B.B. Fuchs, J.V. Spirande","doi":"10.1016/0161-5890(78)90134-7","DOIUrl":null,"url":null,"abstract":"<div><p>The content and the avidity curves of IgM-positive spleen B lymphocytes forming rosettes with TNP-SRBCI were compared in C57BL/6 and BALB/c mice (C57BL/6 × BALB/c)F<sub>1</sub> hybrids, as well as F<sub>1</sub> × C57BL/6 and F<sub>1</sub> × BALB/c backcross hybrids. Conjugates of bovine serum albumin with trinitrophenyl. dinitrophenylsulfonic and sulfanilic acids were used as inhibitors (TNP<sub>24</sub>BSA, DNP<sub>23</sub>BSA, Sulf<sub>17</sub>BSA).</p><p>The spleen TNP-RFC content in BALB/c mice was 60% higher than in the C57BL/6 strain. F<sub>1</sub> hybrids were intermediate in this respect. F<sub>1</sub> × BALB/c hybrids had on the average 35% more TNP/RFC than F<sub>1</sub> × C57BL/6 mice.</p><p>The TNP-RFC inhibition (avidity) curves obtained with TNP<sub>24</sub>BSA, DNP<sub>23</sub>BSA and Sulf<sub>17</sub>BSA were markedly different in BALB/c, C57BL/6 and (BALB/c × C57BL/6)F<sub>1</sub> animals. We conclude that the spleen content and avidity of TNP-RFC in mice are subject to strict genetic control.</p><p>Assuming random expression of V (idiotype?) genes, the present results seem to indicate that the content and proportions of groups of B clones are controlled by structural immunoglobulin genes. The simplest control mechanism could be provided by definite quantitative relationships between corresponding groups of V genes.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":"15 6","pages":"Pages 379-383"},"PeriodicalIF":0.0000,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90134-7","citationCount":"0","resultStr":"{\"title\":\"Genetic control of the content and avidity of hapten-binding B lymphocytes in mouse spleen\",\"authors\":\"B.B. Fuchs, J.V. Spirande\",\"doi\":\"10.1016/0161-5890(78)90134-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The content and the avidity curves of IgM-positive spleen B lymphocytes forming rosettes with TNP-SRBCI were compared in C57BL/6 and BALB/c mice (C57BL/6 × BALB/c)F<sub>1</sub> hybrids, as well as F<sub>1</sub> × C57BL/6 and F<sub>1</sub> × BALB/c backcross hybrids. Conjugates of bovine serum albumin with trinitrophenyl. dinitrophenylsulfonic and sulfanilic acids were used as inhibitors (TNP<sub>24</sub>BSA, DNP<sub>23</sub>BSA, Sulf<sub>17</sub>BSA).</p><p>The spleen TNP-RFC content in BALB/c mice was 60% higher than in the C57BL/6 strain. F<sub>1</sub> hybrids were intermediate in this respect. F<sub>1</sub> × BALB/c hybrids had on the average 35% more TNP/RFC than F<sub>1</sub> × C57BL/6 mice.</p><p>The TNP-RFC inhibition (avidity) curves obtained with TNP<sub>24</sub>BSA, DNP<sub>23</sub>BSA and Sulf<sub>17</sub>BSA were markedly different in BALB/c, C57BL/6 and (BALB/c × C57BL/6)F<sub>1</sub> animals. We conclude that the spleen content and avidity of TNP-RFC in mice are subject to strict genetic control.</p><p>Assuming random expression of V (idiotype?) genes, the present results seem to indicate that the content and proportions of groups of B clones are controlled by structural immunoglobulin genes. The simplest control mechanism could be provided by definite quantitative relationships between corresponding groups of V genes.</p></div>\",\"PeriodicalId\":13265,\"journal\":{\"name\":\"Immunochemistry\",\"volume\":\"15 6\",\"pages\":\"Pages 379-383\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1978-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0161-5890(78)90134-7\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Immunochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0161589078901347\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0161589078901347","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Genetic control of the content and avidity of hapten-binding B lymphocytes in mouse spleen
The content and the avidity curves of IgM-positive spleen B lymphocytes forming rosettes with TNP-SRBCI were compared in C57BL/6 and BALB/c mice (C57BL/6 × BALB/c)F1 hybrids, as well as F1 × C57BL/6 and F1 × BALB/c backcross hybrids. Conjugates of bovine serum albumin with trinitrophenyl. dinitrophenylsulfonic and sulfanilic acids were used as inhibitors (TNP24BSA, DNP23BSA, Sulf17BSA).
The spleen TNP-RFC content in BALB/c mice was 60% higher than in the C57BL/6 strain. F1 hybrids were intermediate in this respect. F1 × BALB/c hybrids had on the average 35% more TNP/RFC than F1 × C57BL/6 mice.
The TNP-RFC inhibition (avidity) curves obtained with TNP24BSA, DNP23BSA and Sulf17BSA were markedly different in BALB/c, C57BL/6 and (BALB/c × C57BL/6)F1 animals. We conclude that the spleen content and avidity of TNP-RFC in mice are subject to strict genetic control.
Assuming random expression of V (idiotype?) genes, the present results seem to indicate that the content and proportions of groups of B clones are controlled by structural immunoglobulin genes. The simplest control mechanism could be provided by definite quantitative relationships between corresponding groups of V genes.
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