Genetic control of the content and avidity of hapten-binding B lymphocytes in mouse spleen

Immunochemistry Pub Date : 1978-06-01 DOI:10.1016/0161-5890(78)90134-7

B.B. Fuchs, J.V. Spirande

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Abstract

The content and the avidity curves of IgM-positive spleen B lymphocytes forming rosettes with TNP-SRBCI were compared in C57BL/6 and BALB/c mice (C57BL/6 × BALB/c)F₁ hybrids, as well as F₁ × C57BL/6 and F₁ × BALB/c backcross hybrids. Conjugates of bovine serum albumin with trinitrophenyl. dinitrophenylsulfonic and sulfanilic acids were used as inhibitors (TNP₂₄BSA, DNP₂₃BSA, Sulf₁₇BSA).

The spleen TNP-RFC content in BALB/c mice was 60% higher than in the C57BL/6 strain. F₁ hybrids were intermediate in this respect. F₁ × BALB/c hybrids had on the average 35% more TNP/RFC than F₁ × C57BL/6 mice.

The TNP-RFC inhibition (avidity) curves obtained with TNP₂₄BSA, DNP₂₃BSA and Sulf₁₇BSA were markedly different in BALB/c, C57BL/6 and (BALB/c × C57BL/6)F₁ animals. We conclude that the spleen content and avidity of TNP-RFC in mice are subject to strict genetic control.

Assuming random expression of V (idiotype?) genes, the present results seem to indicate that the content and proportions of groups of B clones are controlled by structural immunoglobulin genes. The simplest control mechanism could be provided by definite quantitative relationships between corresponding groups of V genes.

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小鼠脾脏半抗原结合B淋巴细胞含量和活性的遗传调控

比较了tnf - srbci在C57BL/6和BALB/c (C57BL/6 × BALB/c)F1杂交种以及F1 × C57BL/6和F1 × BALB/c回交杂交种中igm阳性脾B淋巴细胞形成花环的含量和亲和力曲线。牛血清白蛋白与三硝基苯的结合物。二硝基苯磺酸和磺胺酸作为抑制剂(TNP24BSA, DNP23BSA, Sulf17BSA)。BALB/c小鼠脾脏TNP-RFC含量比C57BL/6小鼠高60%。F1杂交种在这方面处于中间水平。F1 × BALB/c杂交小鼠的TNP/RFC平均比F1 × C57BL/6小鼠高35%。用TNP24BSA、DNP23BSA和Sulf17BSA对BALB/c、C57BL/6和(BALB/c × C57BL/6)F1动物的TNP-RFC抑制(贪婪度)曲线有显著差异。我们认为，小鼠脾脏中TNP-RFC的含量和贪婪程度受到严格的遗传控制。假设V(独特型?)基因的随机表达，目前的结果似乎表明B克隆群的含量和比例是由结构免疫球蛋白基因控制的。最简单的控制机制可能是相应V基因群之间确定的数量关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Immunochemistry

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