Immunochemistry最新文献_第8页

Myelin basic protein serum factor (MBP-SF) in adult lewis rats: A method for detection and evidence that MBP-SF influences the appearance of antibody to MBP in animals developing experimental allergic encephalomyelitis 成年lewis大鼠髓鞘碱性蛋白血清因子(MBP- sf):一种检测方法和证据表明MBP- sf影响实验性变应性脑脊髓炎动物抗MBP抗体的出现

Immunochemistry Pub Date : 1978-07-01 DOI: 10.1016/0161-5890(78)90071-8

Eugene D. Day , Vincent A. Varitek , Philip Y. Paterson

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引用次数: 27

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Immunochemistry Pub Date : 1978-07-01 DOI: 10.1016/0161-5890(78)90084-6

引用次数: 0

Macromolecular insoluble cold globulin (MICG): A novel protein from mouse lymphocytes—II 大分子不溶性冷球蛋白(MICG):一种来自小鼠淋巴细胞的新蛋白

Immunochemistry Pub Date : 1978-07-01 DOI: 10.1016/0161-5890(78)90069-X

Stephen P. Hauptman, Gloria Sobczak, Irvin A. Gutterman

{"title":"Macromolecular insoluble cold globulin (MICG): A novel protein from mouse lymphocytes—II","authors":"Stephen P. Hauptman, Gloria Sobczak, Irvin A. Gutterman","doi":"10.1016/0161-5890(78)90069-X","DOIUrl":"https://doi.org/10.1016/0161-5890(78)90069-X","url":null,"abstract":"<div><p>Macromolecular insoluble cold globulin (MICG) is synthesized selectively by mouse T-cells. In support of this conclusion is evidence derived from mitogenic stimulation of thymic and splenic lymphocytes. PHA and Con A stimulated a disproportionately large increase in MICG compared to total protein synthesis in spleen and thymus cells, while IgM synthesis in spleen cells only rose parallel with the increase in total protein synthesis. In LPS-treated spleen cells, MICG synthesis rose only in proportion to total protein. Therefore, selective enhancement of MICG synthesis, i.e. as a proportion of total protein synthesis, only occurred under conditions where T-cells were activated. In the presence of complement, antibody to MICG was cytotoxic to virtually all thymocytes and half of the spleen cells. Furthermore, antibody to MICG eliminated the mitogenic effect of PHA and Con A on spleen cells, while the response of these lymphocytes to LPS was normal. Cytotoxicity with antibody and complement also caused a significant diminution of MICG synthesis in spleen cells. Finally, isolated T-cells demonstrated a significant amount of MICG synthesis, while only a trace of MICG synthesis was atrributable to B-cells.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":"15 7","pages":"Pages 423-428"},"PeriodicalIF":0.0,"publicationDate":"1978-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90069-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71843736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Effect of lectins on the hemolytic activity of complement components 凝集素对补体成分溶血活性的影响

Immunochemistry Pub Date : 1978-07-01 DOI: 10.1016/0161-5890(78)90075-5

Michael D.P. Boyle, John J. Langone, Tibor Borsos

{"title":"Effect of lectins on the hemolytic activity of complement components","authors":"Michael D.P. Boyle, John J. Langone, Tibor Borsos","doi":"10.1016/0161-5890(78)90075-5","DOIUrl":"10.1016/0161-5890(78)90075-5","url":null,"abstract":"<div><p>We have studied the effect of several lectins that have different sugar specifications on the functional activity of purified guinea-pig and human complement components 1 to 9. We tested castor bean type II (specific for galactose and lactose), wheat germ agglutinin (specific for<em>N</em>-aceytl-glucosamine), lotus bean (specific for fucose), soybean and phytohemagglutinin (PHA) (specific for<em>N</em>-aceytl-<span>d</span>-galactosamine). Leucoagglutinin, a purified constituent of PHA with no known suger specificity, was also included. Soy bean, wheat germ agglutinin and PHA, when immobilized, interacted with GP or Hu C1. Only PHA affected the activity of human or guinea-pig fluid phase Cl. Castor bean type II was found to inhibit the hemolytic action of fluid phase or cell-bound Cl and C4 but inhibited C2 only in the fluid phase. Castor bean type II binds to EACH cells with only minimal impairment of the binding and hemolytic functioning of C2; however, the presence of the lectin inhibited the decay of the hemolytically effective EAC142 sites generated. Lotus bean and leucoagglutinin did not affect the activity of any of the complement components under the conditions tested. Our results suggest that specific sugar residues (e.g. glucose, mannose, lactose or galactose) may play a significant role in the functional activity of human and guinea-pig C1, C4 and C2.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":"15 7","pages":"Pages 465-470"},"PeriodicalIF":0.0,"publicationDate":"1978-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90075-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11772406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Binding affinity of tetrameric coho salmon Ig anti-hapten antibodies 四聚体银鲑Ig抗半抗原抗体的结合亲和力

Immunochemistry Pub Date : 1978-07-01 DOI: 10.1016/0161-5890(78)90074-3

Edward W. Voss Jr. , Warren J. Groberg Jr. , John L. Fryer

引用次数: 32

Macromolecular insoluble cold globulin (MICG): A novel protein from mouse lymphocytes—I 大分子不溶性冷球蛋白(MICG):一种来自小鼠淋巴细胞的新蛋白

Immunochemistry Pub Date : 1978-07-01 DOI: 10.1016/0161-5890(78)90068-8

Stephen P. Hauptman

引用次数: 8

Human secretory component—I. Evidence for a new antigenic specificity 人体分泌成分- i。新的抗原特异性证据

Immunochemistry Pub Date : 1978-06-01 DOI: 10.1016/0161-5890(78)90138-4

S. Iscaki , Colette Geneste , J. Pillot