{"title":"Macromolecular insoluble cold globulin (MICG): A novel protein from mouse lymphocytes—I","authors":"Stephen P. Hauptman","doi":"10.1016/0161-5890(78)90068-8","DOIUrl":null,"url":null,"abstract":"<div><p>Murine thymus and spleen cells synthesize a 225,000 dalton macromolecule (MICG). This protein is insoluble in the cold in non-ionic detergents and migrates as a β-globulin on electrophoresis. MICG can be isolated by a two-step procedure consisting of cold precipitation of cellular lysates in NP-40, followed by gel chromatography in detergent solution. Thymocytes synthesized three times more MICG than splenocytes. Immunological analysis demonstrated that anti-MICG antiserum reacted with the cold insoluble protein. Trypsin digestion of radiolabeled, isolated MICG illustrated the incorporation of<sup>14</sup>C-leucine into a number of peptides. MICG is a glycoprotein which accounts for 3.5% of the protein synthesized in thymus cells and is not secreted from thymocytes or spleen cells.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":"15 7","pages":"Pages 415-422"},"PeriodicalIF":0.0000,"publicationDate":"1978-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90068-8","citationCount":"8","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0161589078900688","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 8
Abstract
Murine thymus and spleen cells synthesize a 225,000 dalton macromolecule (MICG). This protein is insoluble in the cold in non-ionic detergents and migrates as a β-globulin on electrophoresis. MICG can be isolated by a two-step procedure consisting of cold precipitation of cellular lysates in NP-40, followed by gel chromatography in detergent solution. Thymocytes synthesized three times more MICG than splenocytes. Immunological analysis demonstrated that anti-MICG antiserum reacted with the cold insoluble protein. Trypsin digestion of radiolabeled, isolated MICG illustrated the incorporation of14C-leucine into a number of peptides. MICG is a glycoprotein which accounts for 3.5% of the protein synthesized in thymus cells and is not secreted from thymocytes or spleen cells.
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