用改进的方法分离癌胚抗原

Immunochemistry Pub Date : 1978-06-01 DOI:10.1016/0161-5890(78)90135-9

David G. Pritchard , Marianne L. Egan

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引用次数: 8

摘要

本文提出了一种分离癌胚抗原(CEA)的改进方法。在高氯酸萃取之后，经过冗长的透析、超滤和冻干步骤，在色谱纯化步骤之前，用简单的乙醇沉淀CEA代替。用这种方法制备的CEA的化学和免疫化学特性与不使用乙醇沉淀法制备的CEA相比没有显着差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Isolation of carcinoembryonic antigen by an improved procedure

An improved procedure for the isolation of carcinoembryonic antigen (CEA) has been developed. Lengthy dialysis, ultrafiltration and lyophilization steps following perchloric acid extraction are replaced by a simple ethanol precipitation of the CEA prior to the Chromatographie purification steps. Chemical and immunochemical characterization of CEA prepared by this procedure showed no significant differences compared to CEA prepared by the more lengthy procedure not using ethanol precipitation.

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来源期刊

Immunochemistry

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