Immunochemistry最新文献

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Immunological relationship between phycoerythrins from various blue-green algae 不同蓝藻中藻红蛋白的免疫学关系
Immunochemistry Pub Date : 1978-05-01 DOI: 10.1016/0161-5890(78)90092-5
Jo¨rg Eder , Rudolf Wagenmann , Wolfhart Ru¨diger
{"title":"Immunological relationship between phycoerythrins from various blue-green algae","authors":"Jo¨rg Eder ,&nbsp;Rudolf Wagenmann ,&nbsp;Wolfhart Ru¨diger","doi":"10.1016/0161-5890(78)90092-5","DOIUrl":"10.1016/0161-5890(78)90092-5","url":null,"abstract":"<div><p>Rabbit antibodies were prepared against C-phycoerythrin (PE) from a new<em>Pseudanabaena</em> species (strain W 1173) and cross-reactivity of this antiserum with phycoerythrins from various blue-green algae was studied. Qualitative immunochemical analysis indicated similarity between the PE's isolated from the species listed in Table 1. No cross-reaction could be detected with phycocynnin. For quantitative immunological studies a viroimmunoassay was developed. Bacteriophage T4 was chemically modified by covalent attachment of PE from<em>Pseudanabaena</em> W 1173. The immunospecific inactivation of this bacteriophage phycoerythrin conjugate can be inhibited by free phycoerythrin, the degree of inhibition being dependent on the immunochemical similarity between the bound and the free antigen. By means of this technique, substantial differences in inhibitory capacity were found between the various PE's, thus showing that the highly sensitive phage technique is able to discriminate between antigenic differences which cannot be detected by immunodiffusion. The degree of antigenic correspondence was also studied by the method of cross-precipitation, which unlike the phage assay requires the heterologous antigen to be multivalent. As expected, the ranges of cross-reactivity obtained by the two procedures were not identical. This result indicates that theamountof cross-reaction observed and the capacity to distinguish between a series of heterologous antigens is affected by the immunochemical method applied. The potential usefulness of the results for taxonomic purposes is discussed.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1978-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90092-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11319753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Immune response to phosphorylcholine—V 对磷酰胆碱v的免疫反应
Immunochemistry Pub Date : 1978-05-01 DOI: 10.1016/0161-5890(78)90085-8
Gary W. Miller, Heinz Ko¨hler
{"title":"Immune response to phosphorylcholine—V","authors":"Gary W. Miller,&nbsp;Heinz Ko¨hler","doi":"10.1016/0161-5890(78)90085-8","DOIUrl":"https://doi.org/10.1016/0161-5890(78)90085-8","url":null,"abstract":"<div><p>It was recently shown that complement can dissociate BSA-anti-BSA immune complexes (Miller, 1977). In the present study we have formed specific complexes of idiotype and anti-idiotype. The idiotype used was the phosphorylchotine-binding Balb/c myeloma protein, HOPC-8, and the anti-idiotype was raised against purified HOPC-8 protein in A/J mice. Idiotypic complexes were prepared in either of 2 ways: (1) HOPC-8 protein was immobilized in test tubes and allowed to bind purified radiolabeled anti-idiotype. (2) Anti-idiotype was immobilized in test tubes and allowed to bind purified labeled HOPC-8 protein. Then both types of immobilized idiotypic complex were incubated with fresh or heat-inactivated rabbit serum at 37°C. The dissociation of either labeled idiotype or anti-idiotype was measured as a function of time. Active complement increased the rate und extent of dissociation of the idiotypic complex regardless of whether the idiotype or the anti-idiotype was immobilized.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1978-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90085-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71848378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Physicochemical and immunochemical properties of γ1 heavy chain disease protein baz γ - 1重链病蛋白baz的理化和免疫化学性质
Immunochemistry Pub Date : 1978-05-01 DOI: 10.1016/0161-5890(78)90093-7
Linda L. Smith, Betty P. Barton, Fred A. Garver, Lebe S. Chang, Byron McGuire, Guy B. Faguet, C. Lawrence Lutcher
{"title":"Physicochemical and immunochemical properties of γ1 heavy chain disease protein baz","authors":"Linda L. Smith,&nbsp;Betty P. Barton,&nbsp;Fred A. Garver,&nbsp;Lebe S. Chang,&nbsp;Byron McGuire,&nbsp;Guy B. Faguet,&nbsp;C. Lawrence Lutcher","doi":"10.1016/0161-5890(78)90093-7","DOIUrl":"https://doi.org/10.1016/0161-5890(78)90093-7","url":null,"abstract":"<div><p>The physicochemical and antigenic properties of a γ1 heavy chain disease protein (γl HCD BAZ) are described. Protein BAZ is positive for the Glm(1) determinant and gives a reaction of identity with Fc fragment, whether derived from Cohn Fraction II or from an IgG1 myeloma protein. It also gives a reaction of complete identity with the γHCD proteins CRA and ZUC. The mol. wt of the native protein was determined from (1) sedimentation-diffusion, (2) sedimentation-viscosity, and (3) sedimentation-equilibrium measurements to be approx 60,000 daltons. The mol. wt of the unreduced protein in 6<em>M</em> guanidine hydrochloride was found to be 30,000 daltons, which was identical to that of the reduced-alkylated form in 6<em>M</em> guanidine. These results established that γ1 HCD BAZ is a noncovalently linked dimer and suggested the possibility of a missing hinge region.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1978-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90093-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71848395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
A thermodynamic model of binding of flexible bivalent haptens to antibody 柔性二价半抗原与抗体结合的热力学模型
Immunochemistry Pub Date : 1978-05-01 DOI: 10.1016/0161-5890(78)90091-3
Micah Dembo, Byron Goldstein
{"title":"A thermodynamic model of binding of flexible bivalent haptens to antibody","authors":"Micah Dembo,&nbsp;Byron Goldstein","doi":"10.1016/0161-5890(78)90091-3","DOIUrl":"10.1016/0161-5890(78)90091-3","url":null,"abstract":"<div><p>Studies by Wilder<em>et al</em>. (1975<em>b</em>) of the binding of Fab' fragments to small haptens have shown that the cross-linking constant (the equilibrium constant for binding an additional Fab' fragment to a hapten-Fab' complex) is strongly dependent on the length of the hapten. We present a simple model for predicting the relationship between the intermolecular cross-linking constant and the monovalent hapten-antibody binding constant. In particular we use the model to obtain the dependence of the cross-linking constant on the length of the hapten, the depth to which the hapten fills the Fab' binding site, and the size of the Fab' fragment.</p><p>To make contact with experiment, we devise expressions which allow us to analyze the data of Wilder<em>et al</em>. (1975<em>b</em>). From their data we determine the values of two parameters which we take to be unknown in the theory, the size of the Fab' fragment and the depth to which the hapten fills the Fab' binding site. The values arrived at in this way agree well with published measurements of these parameters.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1978-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90091-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11900785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Rabbit antibodies to ovine submaxillary mucin. detection, specificity and cross-reactivity 兔抗羊颌下粘蛋白抗体。检测,特异性和交叉反应性
Immunochemistry Pub Date : 1978-05-01 DOI: 10.1016/0161-5890(78)90096-2
James K. Roche, Eugene D. Day, Hoyle D. Hill
{"title":"Rabbit antibodies to ovine submaxillary mucin. detection, specificity and cross-reactivity","authors":"James K. Roche,&nbsp;Eugene D. Day,&nbsp;Hoyle D. Hill","doi":"10.1016/0161-5890(78)90096-2","DOIUrl":"10.1016/0161-5890(78)90096-2","url":null,"abstract":"<div><p>A newly developed passive hemagglutination inhibition technique was used to show that at least 2 families of antibodies are generated after immunization of rabbits with ovine submaxillary mucin (OSM)§ in homogeneous form. OSM-specific antibodies were non-precipitating in agar and liquid phase, easily detectable by a passive hemagglutination technique, and directed primarily to the carbohydrate side chain. In the case of subcutaneous immunization with OSM emulsified in complete Freund's adjuvant, the immunodominant determinants were the disaccharide prosthetic group (NAN-galNAc-protein) and a desialized moiety (galNAc-protein) which comprises about 14% of total prosthetic groups in OSM as isolated. Modest changes in the terminal sialic acid were associated with reduced interaction with anti-OSM sera. By contrast, intravenous immunization with OSM coupled to red blood cells elicited an additional family of antibodies with specificity for apo-protein. Explanations for this switch in specificity are considered.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1978-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90096-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11301774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
The amino acid sequence of the light chain variable region of a canine myeloma immunoglobulin: Evidence that the vk subgroups predated mammalian speciation 犬骨髓瘤免疫球蛋白轻链可变区氨基酸序列:vk亚群早于哺乳动物物种形成的证据
Immunochemistry Pub Date : 1978-05-01 DOI: 10.1016/0161-5890(78)90090-1
Richard L. Wasserman, J. Donald Capra
{"title":"The amino acid sequence of the light chain variable region of a canine myeloma immunoglobulin: Evidence that the vk subgroups predated mammalian speciation","authors":"Richard L. Wasserman,&nbsp;J. Donald Capra","doi":"10.1016/0161-5890(78)90090-1","DOIUrl":"10.1016/0161-5890(78)90090-1","url":null,"abstract":"<div><p>The complete amino acid sequence of the variable region of a kappa light chain derived from a canine myeloma immunoglobulin is presented. The sequence is more than 80% homologous to the human kappa variable region Cum the prototype of the human V<sub>K</sub>II subgroup and is the first example of a non-human kappa chain which can be easily classified as a subgroup other than V<sub>K</sub>I. The finding confirms a previous prediction that genes encoding kappa subgroups predated mammalian speciation.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1978-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90090-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11319751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
The detection of five antigenically reactive regions in the soybean leghemoglobin a molecule 大豆血红蛋白a分子中5个抗原性反应区的检测
Immunochemistry Pub Date : 1978-05-01 DOI: 10.1016/0161-5890(78)90089-5
John G.R Hurrell , John A. Smith , Sydney J. Leach
{"title":"The detection of five antigenically reactive regions in the soybean leghemoglobin a molecule","authors":"John G.R Hurrell ,&nbsp;John A. Smith ,&nbsp;Sydney J. Leach","doi":"10.1016/0161-5890(78)90089-5","DOIUrl":"10.1016/0161-5890(78)90089-5","url":null,"abstract":"<div><p>By comparison with the amino acid sequence of the sperm whale myoglobin molecule five antigenicaliy reactive regions on the surface of the soybean leghemoglobin a molecule were predicted. The reactivity of these five regions was substantiated using the approach of Smith<em>et al</em>. (1977) in which peptides corresponding to these regions of the molecule were synthesized by a solid-phase procedure on a macroporous resin. At each step of the synthesis the peptides were tested for antigenicity using<sup>125</sup>I-labelled anti-(leghemoglobin a) antibodies whilst still bound to the solid support.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1978-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90089-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11301773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
Characterization of the target cell receptor for IgE—IV. IgE-IV靶细胞受体的表征。
Immunochemistry Pub Date : 1978-05-01 DOI: 10.1016/0161-5890(78)90086-X
D.H. Conrad , A. Froese
{"title":"Characterization of the target cell receptor for IgE—IV.","authors":"D.H. Conrad ,&nbsp;A. Froese","doi":"10.1016/0161-5890(78)90086-X","DOIUrl":"https://doi.org/10.1016/0161-5890(78)90086-X","url":null,"abstract":"<div><p>Complexes, consisting of IgE and its receptor from rat basophilic leukemia (RBL) cells, were isolated by allowing RBL cells to interact with DNP-IgE, solubilizing the cells with Nonidet P-40 and by allowing the extract to bind to anti-DNP-Sepharose. Of the RBL surface material eluted with 2,4-dinitrophenolate, 51–65% was precipitable by anti-IgE. Polyacrylamide gel analysis of eluates in SDS. using 10% gels, revealed a single surface component with an apparent molecular weight of about 45.000 daltons.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1978-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90086-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71845260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Encephalitogenicity: A proposed mechanism 致脑性:一种被提出的机制
Immunochemistry Pub Date : 1978-05-01 DOI: 10.1016/0161-5890(78)90088-3
Fred C. Westall
{"title":"Encephalitogenicity: A proposed mechanism","authors":"Fred C. Westall","doi":"10.1016/0161-5890(78)90088-3","DOIUrl":"10.1016/0161-5890(78)90088-3","url":null,"abstract":"<div><p>A theory is proposed suggesting that one exposed region of the myelin basic protein<em>in vivo</em> might be the immunological target for cells which have been stimulated by several different encephalitogenic determinants.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1978-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90088-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11301771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chemiluminescence immunoassay; A new sensitive method for determination of antigens 化学发光免疫测定;一种灵敏的抗原测定新方法
Immunochemistry Pub Date : 1978-05-01 DOI: 10.1016/0161-5890(78)90094-9
Baruch Velan, Mirjam Halmann
{"title":"Chemiluminescence immunoassay; A new sensitive method for determination of antigens","authors":"Baruch Velan,&nbsp;Mirjam Halmann","doi":"10.1016/0161-5890(78)90094-9","DOIUrl":"10.1016/0161-5890(78)90094-9","url":null,"abstract":"<div><p>A chemiluminescent immunoassay was developed using peroxidase labeled antigen. The high sensitivity obtained permitted the determination of 1 ng staphylococcal enterotoxin B used as test substance.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1978-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90094-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11900786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 26
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