IEEE Transactions on NanoBioscience最新文献

{"title":"Improved Destaining and Antimicrobial Potential of Pepsin Cross-Linked HPMC-Se Nanoparticles","authors":"Taleeha Roheen;Nazia Perveen;Nasir Assad;Muhammad Fayyaz ur Rehman;Muhammad Nadeem;Farhan Ahmad Atif;Humaira Yasmeen Gondal;Fozia Batool;Mehvish Bibi;Shahzad;Ahmad Bilal;Rehana Kousar;Noor ul Ain Zafar;Daneen Gondal","doi":"10.1109/TNB.2025.3648368","DOIUrl":"10.1109/TNB.2025.3648368","url":null,"abstract":"In this study, Hydroxypropyl methyl cellulose-stabilized selenium nanoparticles (HPMC-SeNPs) were successfully synthesized and utilized as a support for pepsin immobilization via glutaraldehyde crosslinking. Characterization through SEM, FTIR, and XRD confirmed their structural integrity, while zeta potential (- 12.61 mV) and DLS (PDI: 0.1818) indicated good colloidal stability and uniform size distribution. The immobilized pepsin (HPMC/Se-Pep) demonstrated high immobilization efficiency (81.25%) and yield (78.73%). The enzymatic system exhibited enhanced stability over a wider pH (2–6) and temperature range (20–<inline-formula> <tex-math>$60~^{circ }$ </tex-math></inline-formula>C), with improved kinetic parameters—lower K_m (0.16 mM) and higher V_max (<inline-formula> <tex-math>$0.94~mu $ </tex-math></inline-formula>mol/min)—indicating stronger substrate affinity and better catalytic performance than free pepsin. Functional assays revealed significantly enhanced caseinolytic and antimicrobial activities, with inhibition zones of <inline-formula> <tex-math>$22~pm ~0.05$ </tex-math></inline-formula> mm (<italic>S. aureus</i>) and <inline-formula> <tex-math>$21~pm ~0.12$ </tex-math></inline-formula> mm (<italic>E. coli</i>), outperforming both native NPs and free enzyme. Notably, the immobilized pepsin achieved complete removal of blood stains within 30–40 min and retained 60.2% of its activity after five reuse cycles, confirming excellent operational stability. However, a gradual decline in enzymatic activity was observed after repeated reuse and prolonged storage, indicating the need for further optimisation to enhance long-term stability. These results demonstrate that HPMC-SeNPs are an effective platform for enzyme immobilization, offering improved performance, reusability, and multifunctionality. The developed system holds strong potential for applications in biocatalysis, textile processing, and healthcare industries.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"25 2","pages":"213-221"},"PeriodicalIF":4.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145843778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IEEE Transactions on NanoBioscience Information for Authors","authors":"","doi":"10.1109/TNB.2025.3643101","DOIUrl":"https://doi.org/10.1109/TNB.2025.3643101","url":null,"abstract":"","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"25 1","pages":"C3-C3"},"PeriodicalIF":4.4,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=11320946","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145861214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IEEE Transactions on NanoBioscience Publication Information","authors":"","doi":"10.1109/TNB.2025.3643097","DOIUrl":"https://doi.org/10.1109/TNB.2025.3643097","url":null,"abstract":"","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"25 1","pages":"C2-C2"},"PeriodicalIF":4.4,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=11320925","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145861194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Graphene Oxide-Based Acetamiprid Detection Platform and the Construction of Logic Gate","authors":"Xuemei Yan;Sunfan Xi;Can Qiao;Zhenliang Zhang;Yidan Sang;Mengyang Hu;Yafei Dong;Luhui Wang","doi":"10.1109/TNB.2025.3626826","DOIUrl":"10.1109/TNB.2025.3626826","url":null,"abstract":"Pesticides are widely used in food cultivation and have become one of the most important means of ensuring the development of agriculture, forestry and animal husbandry. At the same time, pesticide residue detection technology needs to be updated to provide a scientific and effective basis to cope with the problems arising from the use of pesticides. As a new type of nicotine insecticide, acetamiprid is widely used in agricultural production. This paper reports a fluorescent biosensor based on graphene oxide and G-quadruplex that can be used for the determination of the target acetamiprid. It contains a specific DNA probe that can form the G-quadruplex structure and the nucleic acid sequence of the acetamiprid aptamer as the main element. The probe is stabilized and adsorbed by the<inline-formula> <tex-math>$pi $ </tex-math></inline-formula>-<inline-formula> <tex-math>$pi $ </tex-math></inline-formula> interaction of graphene, achieving the variation of the assay results. The optimal sequences designed for rapid screening of nucleic acids were modeled and simulated using bioinformatics tools such as NUPACK and AutoDock prior to the experiment. The detection limit of acetamiprid was 165.5 pM, which was much lower than the national food safety standard residue of 0.05 mg/kg. The sensor has the advantages of obvious economic value, fewer steps and shorter detection time. It has great application prospects in the field of food safety.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"25 1","pages":"120-126"},"PeriodicalIF":4.4,"publicationDate":"2025-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145471118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized Tri-Coil Magnetic Guidance of Nanorobots for Targeted Plaque Therapy in the Middle Cerebral Artery","authors":"Hazrat Bilal;M. S. Aslam;Aaiza Gul;Aakash Kumar;Inam Ullah;Athanasios V. Vasilakos","doi":"10.1109/TNB.2025.3629132","DOIUrl":"10.1109/TNB.2025.3629132","url":null,"abstract":"Intravascular nanorobotic interventions in the neurovasculature are a promising yet challenging frontier in medical technology. This study addresses the challenge of precise control over nanorobots within the Middle Cerebral Artery (MCA), particularly under conditions of significant stenosis (80.1%) that complicate flow and navigation. Despite advances in magnetically actuated nanorobots for targeted therapies, achieving fine-grained control in complex vascular environments remains a critical issue. To address this, we propose a novel tri-coil electromagnetic system that uses three independent coils to generate dynamically modulated magnetic fields, enabling precise control of nanorobot motion. Coils 1 and 3 create evolving magnetic fields, while Coil 2 maintains a static field to shape the overall force application. A mathematical model was developed and implemented to optimize the system, demonstrating its ability to manipulate nanorobots within stenosed M2 MCA vessels. Our approach achieved high precision, allowing a lateral shift of the nanorobot trajectory with a magnetic field intensity of 40 mT. This tri-coil system offers a significant advancement in nanorobotic navigation and treatment of cerebrovascular diseases through minimally invasive techniques.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"25 1","pages":"127-139"},"PeriodicalIF":4.4,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145451647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Practical Transmitters for MC: Functionalized Nanodevices Employing Cooperative Transmembrane Transport Proteins","authors":"Teena tom Dieck;Lukas Brand;Lea Erbacher;Daniela Wegner;Sebastian Lotter;Kathrin Castiglione;Robert Schober;Maximilian Schäfer","doi":"10.1109/TNB.2025.3627286","DOIUrl":"10.1109/TNB.2025.3627286","url":null,"abstract":"This paper presents a novel optically controllable molecular communication (MC) transmitter (TX) design based on vesicular nanodevices (NDs), functionalized for controlled signaling molecule release via transmembrane proteins. All system components are chemically realizable, bridging the gap between MC theory and practical implementation. The NDs enable optical-to-chemical signal conversion, making them suitable as externally controllable TXs in various MC systems. The proposed design comprises two cooperating modules, namely an energizing and a release module, allowing the release of different signaling molecules depending on the module configuration. We introduce a general system model and provide a detailed mathematical analysis of a specific TX realization, deriving both exact and approximate analytical expressions for the released signaling molecule concentration, which are validated via numerical methods. The proposed model also accounts for the impact of buffering media commonly present in experimental or in-body environments. We further incorporate the impact of multiple NDs and parameter randomness inherent to vesicle synthesis into our model. The proposed models for single and multiple ND scenarios enable system parameter optimization, aiding the future experimental realization of the proposed MC TXs.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"25 1","pages":"80-98"},"PeriodicalIF":4.4,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=11222784","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145408857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetomechanical Force-Driven Cell Permeabilization via Pulsed Magnetic Field and Magnetic Nanoparticles","authors":"Chi Ma;Wei Zheng;Fei Teng;Sifan Tang;Jianli Wang;Jiayu Chen;Yan Mi","doi":"10.1109/TNB.2025.3615613","DOIUrl":"10.1109/TNB.2025.3615613","url":null,"abstract":"As a non-contact physical intervention technique, pulsed magnetic field (PMF) has been shown to regulate cell membrane permeability. However, the underlying mechanism remains unclear, and their permeabilization efficiency is relatively low. Building on the advantages of magneto-mechanical regulation with magnetic nanoparticles, this study proposes combining PMF with magnetic nanoparticles. By leveraging magneto-mechanical force (MMF) as the central mechanism, the aim is to enhance cell permeabilization rate through optimization of the applied force magnitude. First, a theoretical analysis of the forces acting on magnetic nanoparticles was performed to guide particle parameter selection. Next, the effects of PMF alone and its combination with magnetic nanoparticles on cell membrane permeability were examined through in vitro experiments. Finally, fluorescence probes were used to investigate the biochemical mechanisms underlying cell permeabilization induced by both treatments. The permeabilization experiment results showed that the combined treatment significantly enhanced cell permeabilization. Compared to PMF treatment alone, the half-maximal effective dose decreased by 27.85%, and the rate of change in permeabilization rate increased by 49.7%. Fluorescence staining further revealed that, unlike the biochemical pathways activated by PMF treatment alone, the combined treatment caused multiple disruptions in cytoskeletal microfilaments, confirming that it induced cell permeabilization through a physical mechanism involving mechanical stress. This study leveraged the MMF generated by magnetic nanoparticles under PMF to regulate cell membrane permeability, providing a novel approach for precise control of cell membrane permeability based on physical parameters.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"25 1","pages":"108-119"},"PeriodicalIF":4.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IEEE Transactions on NanoBioscience Publication Information","authors":"","doi":"10.1109/TNB.2025.3608880","DOIUrl":"https://doi.org/10.1109/TNB.2025.3608880","url":null,"abstract":"","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 4","pages":"C2-C2"},"PeriodicalIF":4.4,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=11180169","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145134913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IEEE Transactions on NanoBioscience Information for Authors","authors":"","doi":"10.1109/TNB.2025.3608884","DOIUrl":"https://doi.org/10.1109/TNB.2025.3608884","url":null,"abstract":"","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 4","pages":"C3-C3"},"PeriodicalIF":4.4,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=11180170","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145134930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silence-Based Multi-Type Hybrid Transmission Scheme for Mobile Molecular Communication System","authors":"Nihit Bhatnagar;Aneerban Roy;Sandeep Joshi","doi":"10.1109/TNB.2025.3610622","DOIUrl":"10.1109/TNB.2025.3610622","url":null,"abstract":"In this work, we consider a three-dimensional slow diffusive heterogeneous media-based mobile molecular communication (MC) system, with the communicating devices as point transmitters and passive spherical-shaped receiver nanomachines (NMs). For the considered slow diffusive MC system, we propose a time-varying stochastic diffusivity-based model for communicating devices and information-carrying molecules, and we characterize the mobile MC channel by the channel impulse response (CIR) and derive its mean. For the considered slow and stochastic diffusivity-based mobile MC system, we propose a novel silence-based multi-type hybrid transmission scheme, which combines communication through silence (CtS) with molecular shift keying (MoSK) and concentration shift keying (CSK) and we derive the closed-form expression for the average probability of error. For the slow diffusive environment, we compare the proposed transmission scheme with the position and concentration-based run-length aware, MoSK, and CSK transmission schemes. For the proposed silence-based multi-type hybrid and considered position and concentration-based run-length aware transmission schemes, we design their respective maximum likelihood (ML) threshold detectors. The proposed scheme outperforms and shows robust behavior in the presence of inter-symbol interference.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"25 1","pages":"70-79"},"PeriodicalIF":4.4,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145075109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}