IEEE Transactions on NanoBioscience最新文献_第10页

Electrochemical Synthesis of Molecularly Imprinted Polymers for L-Tyrosine Detection 用于检测 L-酪氨酸的分子印迹聚合物的电化学合成。

IF 3.7 4区生物学

IEEE Transactions on NanoBioscience Pub Date : 2024-03-20 DOI: 10.1109/TNB.2024.3379588

K. Ramya;K. S. Jaya Lakshmi;Khairunnisa Amreen;Sanket Goel

{"title":"Electrochemical Synthesis of Molecularly Imprinted Polymers for L-Tyrosine Detection","authors":"K. Ramya;K. S. Jaya Lakshmi;Khairunnisa Amreen;Sanket Goel","doi":"10.1109/TNB.2024.3379588","DOIUrl":"10.1109/TNB.2024.3379588","url":null,"abstract":"L-Tyrosine (L-Tyr), a critical amino acid whose aberrant levels impact melanin and dopamine levels in human body while also increasing insulin resistance thereby increasing the risk of type 2 diabetes. The objective of this study was to detect the amount of L-Tyr in human fluids by tailored electrochemical synthesis of well adhered, homogenous and thin molecularly imprinted polymers (MIPs) by the electro-polymerization of pyrrole on glassy carbon electrode modified functionalized multi-walled carbon nanotubes. The key benefits of this procedure over previous imprinting techniques were the elimination of expensive materials like Au and tedious multi-step synthesis, for L-Tyr detection using a handheld potentiostat. The developed particles were characterized using Fourier Transform Infrared Spectroscopy, Scanning Electron Microscope, Chronoamperometry, and Cyclic Voltammetry. With strong reproducibility and stability, this optimized approach provides a rapid and effective method of preparing and sensing MIPs for the target analyte with a broad linear range of \u0000<inline-formula> <tex-math>$1~mu text{M}$ </tex-math></inline-formula>\u0000 to \u0000<inline-formula> <tex-math>$2000~mu text{M}$ </tex-math></inline-formula>\u0000. The Limit of Detection and Limit of Quantification were \u0000<inline-formula> <tex-math>$0.4~mu text{M}$ </tex-math></inline-formula>\u0000 and \u0000<inline-formula> <tex-math>$1.47~mu text{M}$ </tex-math></inline-formula>\u0000, respectively. The engineered sensor was validated for quantifying the concentrations of L-Tyr in human blood and serum samples, yielding satisfactory recovery and can be expanded in future to detect analytes simultaneous.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"23 3","pages":"410-417"},"PeriodicalIF":3.7,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140174505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Label Free Fluorescent Aptamer Sensor Based on the Combined Action of Graphene Oxide and SYBR Green I for the Detection of Aflatoxin B1 基于氧化石墨烯和 SYBR Green I 联合作用的无标记荧光拟合传感器，用于检测黄曲霉毒素 B1。

IF 3.7 4区生物学

IEEE Transactions on NanoBioscience Pub Date : 2024-03-20 DOI: 10.1109/TNB.2024.3403158

Mengyang Hu;Meng Cheng;Na Wang;Yidan Sang;Yafei Dong;Luhui Wang

{"title":"A Label Free Fluorescent Aptamer Sensor Based on the Combined Action of Graphene Oxide and SYBR Green I for the Detection of Aflatoxin B1","authors":"Mengyang Hu;Meng Cheng;Na Wang;Yidan Sang;Yafei Dong;Luhui Wang","doi":"10.1109/TNB.2024.3403158","DOIUrl":"10.1109/TNB.2024.3403158","url":null,"abstract":"Here, based on the characteristics of Graphene oxide(GO) and SYBR Green I(SGI) dye, an enzyme-free and label-free fluorescent biosensor with signal amplification through DNA strand reaction is proposed for the detection of Aflatoxin B1(AFB1) in food safety. Firstly, without the addition of AFB1, the substrate in the system includes a double stranded Apt-S with a long sticky end and two hairpins H1 and H2. Although the complementary pairing of bases may exhibit fluorescence due to the insertion of SGI dyes, the use of GO, which is highly capable of adsorbing single stranded parts and quenching fluorescence, cleverly reduces the background fluorescence. Adding the target AFB1 triggers DNA inter chain reactions, generating a large amount of long double stranded DNA H1-H2, thereby generating strong fluorescence signals under the action of SGI. More importantly, logical theory verification and computer simulation were conducted before biological experiments, providing a theoretical basis for the implementation of the biosensor. After analysis, the fluorescence biosensor exhibits a good linear relationship with AFB1 concentration in the range of 5-50nM, with a detection limit of 0.76nM. It also has good specificity, anti-interference ability, and practical application ability, and has broad application prospects in the field of food safety.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 1","pages":"37-45"},"PeriodicalIF":3.7,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance Analysis and ISI Mitigation With Imperfect Transmitter in Molecular Communication 分子通信中不完美发射机的性能分析和 ISI 缓解。

IF 3.7 4区生物学

IEEE Transactions on NanoBioscience Pub Date : 2024-03-14 DOI: 10.1109/TNB.2024.3375933

Dongliang Jing;Lin Lin;Andrew W. Eckford

引用次数: 0

Analysis of Tapered Fibre Optic Surface Plasmon Resonance Bio-Sensor Chip With Highly Perturbed Taper Profiles 分析锥形光纤表面等离子体共振生物传感器芯片的高扰动锥形轮廓。

IF 3.7 4区生物学

IEEE Transactions on NanoBioscience Pub Date : 2024-03-13 DOI: 10.1109/TNB.2024.3376824

Sanjeev Kumar Raghuwanshi

引用次数: 0

Biomedical Applications of Green Synthesized Zinc Oxide and Magnesium-Doped Zinc Oxide Nanoparticles Using Aqueous Extract of Ziziphus Oxyphylla Leaves 利用氧化锌叶水提取物绿色合成氧化锌和掺镁氧化锌纳米粒子的生物医学应用

IF 3.7 4区生物学

IEEE Transactions on NanoBioscience Pub Date : 2024-03-12 DOI: 10.1109/TNB.2024.3373777

Suliman Syed;Arshad Islam;Muhammad Shabeer;Akhtar Nadhman;Farhan Ahmad;Nadia Irfan;Shaila Mehwish;Ajmal Khan

{"title":"Biomedical Applications of Green Synthesized Zinc Oxide and Magnesium-Doped Zinc Oxide Nanoparticles Using Aqueous Extract of Ziziphus Oxyphylla Leaves","authors":"Suliman Syed;Arshad Islam;Muhammad Shabeer;Akhtar Nadhman;Farhan Ahmad;Nadia Irfan;Shaila Mehwish;Ajmal Khan","doi":"10.1109/TNB.2024.3373777","DOIUrl":"10.1109/TNB.2024.3373777","url":null,"abstract":"Zinc oxide (ZnO) and magnesium-doped zinc oxide (Mg-doped ZnO) nanoparticles (NPs) were synthesized using Ziziphus oxyphylla ’s aqueous leaf extract as reducing agent. UV-Vis absorption peaks at 324 nm and 335 nm were indicative of ZnO and Mg-doped ZnO, respectively. FTIR absorption bands observed at 3238, 1043, 1400, 1401, 2186 and 2320 cm\u0000<inline-formula> <tex-math>$^{-1}$ </tex-math></inline-formula>\u0000 suggested the presence of phenols, alcohols, saturated hydrocarbons, and possibly alkynes. X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) spectroscopy revealed pure, spherical and agglomerated NPs with average size of 35.9 nm (ZnO) and 56.8 nm (Mg-doped ZnO). Both NPs remained active against all bacterial strains with the highest inhibition zones observed against Proteus vulgaris (21.16±1.25 mm for ZnO and 24.1±0.76 mm for Mg-doped ZnO. EtBr fluorescence (cartwheel assay) indicated efflux pump blockage, suggesting its facilitation in the bacterial growth inhibition. Antioxidant potential, determined via DPPH radical scavenging assay, revealed stronger antioxidant potential for Mg-doped ZnO (IC\u0000<inline-formula> <tex-math>$_{{50}}~21.53pm 0.76~mu text{g}$ </tex-math></inline-formula>\u0000/mL) than pure ZnO (IC\u0000<inline-formula> <tex-math>$_{{50}}~30.32pm 0.73~mu text{g}$ </tex-math></inline-formula>\u0000/mL). Furthermore, both NPs showed antileishmanial activity against Leishmania tropica promastigotes (IC\u0000<inline-formula> <tex-math>$_{{50}}~47.23pm 3.22~mu text{g}$ </tex-math></inline-formula>\u0000/mL for Mg-doped ZnO and 64.34±6.56 for ZnO), while neither NP exhibited significant hemolysis, indicating biocompatibility and further assessment for their drugability.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"23 3","pages":"418-427"},"PeriodicalIF":3.7,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140049315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Situ Measurement of Urea Concentration With an In-Fiber SPR-MZI Sensor 利用纤维内 SPR-MZI 传感器现场测量尿素浓度。

IF 3.7 4区生物学

IEEE Transactions on NanoBioscience Pub Date : 2024-03-09 DOI: 10.1109/TNB.2024.3398807

Liangliang Cheng;Wanlu Zheng;Ya-Nan Zhang;Xuegang Li;Yong Zhao

引用次数: 0

On the Target Detection Performance of a Molecular Communication Network With Multiple Mobile Nanomachines 关于具有多个移动纳米机器的分子通信网络的目标探测性能。

IF 3.7 4区生物学

IEEE Transactions on NanoBioscience Pub Date : 2024-03-09 DOI: 10.1109/TNB.2024.3399188

Nithin V. Sabu;Abhishek K. Gupta

{"title":"On the Target Detection Performance of a Molecular Communication Network With Multiple Mobile Nanomachines","authors":"Nithin V. Sabu;Abhishek K. Gupta","doi":"10.1109/TNB.2024.3399188","DOIUrl":"10.1109/TNB.2024.3399188","url":null,"abstract":"A network of nanomachines (NMs) can be used to build a target detection system for a variety of promising applications. They have the potential to detect toxic chemicals, infectious bacteria, and biomarkers of dangerous diseases such as cancer within the human body. Many diseases and health disorders can be detected early and efficiently treated in the future by utilizing these systems. To fully grasp the potential of these systems, mathematical analysis is required. This paper describes an analytical framework for modeling and analyzing the performance of target detection systems composed of multiple mobile nanomachines of varying sizes with passive/absorbing boundaries. We consider both direct contact detection, in which NMs must physically contact the target to detect it, and indirect sensing, in which NMs must detect the marker molecules emitted by the target. The detection performance of such systems is calculated for degradable and non-degradable targets, as well as mobile and stationary targets. The derived expressions provide various insights, such as the effect of NM density and target degradation on detection probability.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"23 3","pages":"524-536"},"PeriodicalIF":3.7,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A High-Throughput Microdroplet-Based Single Cell Transfection Method for Gene Knockout Based on the CRISPR/Cas9 System 基于 CRISPR/Cas9 系统的基因敲除高通量微滴单细胞转染方法。

IF 3.9 4区生物学

IEEE Transactions on NanoBioscience Pub Date : 2024-03-07 DOI: 10.1109/TNB.2024.3373597

Jiayu Sun;Yang Jiao;Fei Pan;Shuk Han Cheng;Dong Sun

{"title":"A High-Throughput Microdroplet-Based Single Cell Transfection Method for Gene Knockout Based on the CRISPR/Cas9 System","authors":"Jiayu Sun;Yang Jiao;Fei Pan;Shuk Han Cheng;Dong Sun","doi":"10.1109/TNB.2024.3373597","DOIUrl":"10.1109/TNB.2024.3373597","url":null,"abstract":"The efficient application of the newly developed gene-editing method CRISPR/Cas9 requires more accurate intracellular gene delivery. Traditional delivery approaches, such as lipotransfection and non-viral delivery methods, must contend with major problems to overcome the drawbacks of low efficiency, high toxicity, and cell-type dependency. The high-throughput microdroplet-based single-cell transfection method presented herein provides an alternative method for delivering genome-editing reagents into single living cells. By accurately controlling the number of exogenous plasmids in microdroplets, this method can achieve high-efficiency delivery of nucleic acids to different types of single cells. This paper presents a high-throughput quantitative DNA transfection method for single cells and explores the optimal DNA transfection conditions for specific cell lines. The transfection efficiency of cells at different concentrations of DNA in microdroplets is measured. Under the optimized transfection conditions, the method is used to construct gene-knockout cancer cell lines to determine specific gene functions through the CRISPR/Cas9 knockout system. In a case study, the migration ability of TRIM72 knockout cancer cells is inhibited, and the tumorigenicity of cells in a zebrafish tumor model is reduced. A single-cell microfluidic chip is designed to achieve CRISPR/Cas9 DNA transfection, dramatically improving the transfection efficiency of difficult-to-transfect cells. This research demonstrates that the microdroplet method developed herein has a unique advantage in CRISPR/Cas9 gene-editing applications.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"23 2","pages":"378-388"},"PeriodicalIF":3.9,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140039243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Potential of DNA Computing for Complex Big Data Problems: A Case Study on the Traveling Car Renter Problem 探索 DNA 计算在解决复杂大数据问题方面的潜力：旅行租车问题案例研究》。

IF 3.7 4区生物学

IEEE Transactions on NanoBioscience Pub Date : 2024-03-06 DOI: 10.1109/TNB.2024.3396142

Zhao-Cai Wang;Xian Wu;Kun Liang;Tun-Hua Wu

{"title":"Exploring the Potential of DNA Computing for Complex Big Data Problems: A Case Study on the Traveling Car Renter Problem","authors":"Zhao-Cai Wang;Xian Wu;Kun Liang;Tun-Hua Wu","doi":"10.1109/TNB.2024.3396142","DOIUrl":"10.1109/TNB.2024.3396142","url":null,"abstract":"The traveling car renter problem (TCRP) is a variant of the Traveling Salesman Problem (TSP) wherein the salesman utilizes rented cars for travel. The primary objective of this problem is to identify a solution that minimizes the cumulative operating costs. Given its classification as a non-deterministic polynomial (NP) problem, traditional computers are not proficient in effectively resolving it. Conversely, DNA computing exhibits unparalleled advantages when confronted with NP-hard problems. This paper presents a DNA algorithm, based on the Adleman-Lipton model, as a proposed approach to address TCRP. The solution for TCRP can be acquired by following a series of fundamental steps, including coding, interaction, and extraction. The time computing complexity of the proposed DNA algorithm is \u0000<inline-formula> <tex-math>$boldsymbol {O}(boldsymbol {n}^{boldsymbol {2}}boldsymbol {m})$ </tex-math></inline-formula>\u0000 for TCRP with n cities and m types of cars. By conducting simulation experiments, the solutions for certain instances of TCRP are computed and compared to those obtained by alternative algorithms. The proposed algorithm further illustrates the potential of DNA computing, as a form of parallel computing, to address more intricate large-scale problems.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"23 3","pages":"391-402"},"PeriodicalIF":3.7,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140854996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterizations of Centrifugal Electrospun Polyvinyl Alcohol/Sodium Alginate/Tamanu Oil/Silver Nanoparticles Wound Dressing 离心电纺聚乙烯醇/海藻酸钠/柚木油/银纳米粒子伤口敷料的特性。

IF 3.9 4区生物学

IEEE Transactions on NanoBioscience Pub Date : 2024-03-01 DOI: 10.1109/TNB.2024.3371224

Thi Phuong Anh Tran;Anh Hue Luong;Wei-Chih Lin

{"title":"Characterizations of Centrifugal Electrospun Polyvinyl Alcohol/Sodium Alginate/Tamanu Oil/Silver Nanoparticles Wound Dressing","authors":"Thi Phuong Anh Tran;Anh Hue Luong;Wei-Chih Lin","doi":"10.1109/TNB.2024.3371224","DOIUrl":"10.1109/TNB.2024.3371224","url":null,"abstract":"Known for its water solubility, flexibility, strong adhesion, and eco-friendly nature, polyvinyl alcohol (PVA) is widely used in various industries. In the medical field, it is used for applications such as creating bandages and orthopaedic devices. Incorporating sodium alginate (SA) into PVA membranes enhances their structural integrity, breathability, and permeability, thereby minimising the risk of cellular damage in the wound zone. Moreover, the addition of tamanu oil (C alophyllum inophyllum L.) and silver nanoparticles, both of which are known for their antibacterial properties and benefits in traditional wound healing, further enhances the membranes’ wound-healing effectiveness. Following production, the membranes undergo a series of tests designed to evaluate their physical properties as well as their antioxidant and antibacterial capabilities. Subsequently, in vitro testing is conducted using human skin cells; experiments on Wistar rats are then performed. Numerous experiments have consistently demonstrated that the performance of polyvinyl alcohol/sodium alginate/tamanu oil (PVA/SA/Oil) membrane is superior to that of polyvinyl alcohol/sodium alginate/tamanu oil/silver nanoparticles (PVA/SA/Oil/Ag NP) membrane. Specifically, the polyvinyl alcohol/sodium alginate (PVA/SA) combination exhibits an impressive wound-healing rate of 98.82% after 15 days, with cells maintaining a high viability of 92% in a nourishing environment. Moreover, these membranes exhibit exceptional resistance to the oxidation of free radicals, surpassing the 70% threshold, and they possess antibacterial activity against Staphylococcus aureus subsp. aureus in vitro. Based on the obtained results, the nanofiber membranes composed of polyvinyl alcohol/ alginate/ tamanu oil, with or without silver nanoparticles, have shown potential as wound dressings in the wound care discipline.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"23 2","pages":"368-377"},"PeriodicalIF":3.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=10457035","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140012540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0