K. Ramya;K. S. Jaya Lakshmi;Khairunnisa Amreen;Sanket Goel
{"title":"用于检测 L-酪氨酸的分子印迹聚合物的电化学合成。","authors":"K. Ramya;K. S. Jaya Lakshmi;Khairunnisa Amreen;Sanket Goel","doi":"10.1109/TNB.2024.3379588","DOIUrl":null,"url":null,"abstract":"L-Tyrosine (L-Tyr), a critical amino acid whose aberrant levels impact melanin and dopamine levels in human body while also increasing insulin resistance thereby increasing the risk of type 2 diabetes. The objective of this study was to detect the amount of L-Tyr in human fluids by tailored electrochemical synthesis of well adhered, homogenous and thin molecularly imprinted polymers (MIPs) by the electro-polymerization of pyrrole on glassy carbon electrode modified functionalized multi-walled carbon nanotubes. The key benefits of this procedure over previous imprinting techniques were the elimination of expensive materials like Au and tedious multi-step synthesis, for L-Tyr detection using a handheld potentiostat. The developed particles were characterized using Fourier Transform Infrared Spectroscopy, Scanning Electron Microscope, Chronoamperometry, and Cyclic Voltammetry. With strong reproducibility and stability, this optimized approach provides a rapid and effective method of preparing and sensing MIPs for the target analyte with a broad linear range of \n<inline-formula> <tex-math>$1~\\mu \\text{M}$ </tex-math></inline-formula>\n to \n<inline-formula> <tex-math>$2000~\\mu \\text{M}$ </tex-math></inline-formula>\n. The Limit of Detection and Limit of Quantification were \n<inline-formula> <tex-math>$0.4~\\mu \\text{M}$ </tex-math></inline-formula>\n and \n<inline-formula> <tex-math>$1.47~\\mu \\text{M}$ </tex-math></inline-formula>\n, respectively. The engineered sensor was validated for quantifying the concentrations of L-Tyr in human blood and serum samples, yielding satisfactory recovery and can be expanded in future to detect analytes simultaneous.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Electrochemical Synthesis of Molecularly Imprinted Polymers for L-Tyrosine Detection\",\"authors\":\"K. Ramya;K. S. Jaya Lakshmi;Khairunnisa Amreen;Sanket Goel\",\"doi\":\"10.1109/TNB.2024.3379588\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"L-Tyrosine (L-Tyr), a critical amino acid whose aberrant levels impact melanin and dopamine levels in human body while also increasing insulin resistance thereby increasing the risk of type 2 diabetes. The objective of this study was to detect the amount of L-Tyr in human fluids by tailored electrochemical synthesis of well adhered, homogenous and thin molecularly imprinted polymers (MIPs) by the electro-polymerization of pyrrole on glassy carbon electrode modified functionalized multi-walled carbon nanotubes. The key benefits of this procedure over previous imprinting techniques were the elimination of expensive materials like Au and tedious multi-step synthesis, for L-Tyr detection using a handheld potentiostat. The developed particles were characterized using Fourier Transform Infrared Spectroscopy, Scanning Electron Microscope, Chronoamperometry, and Cyclic Voltammetry. With strong reproducibility and stability, this optimized approach provides a rapid and effective method of preparing and sensing MIPs for the target analyte with a broad linear range of \\n<inline-formula> <tex-math>$1~\\\\mu \\\\text{M}$ </tex-math></inline-formula>\\n to \\n<inline-formula> <tex-math>$2000~\\\\mu \\\\text{M}$ </tex-math></inline-formula>\\n. The Limit of Detection and Limit of Quantification were \\n<inline-formula> <tex-math>$0.4~\\\\mu \\\\text{M}$ </tex-math></inline-formula>\\n and \\n<inline-formula> <tex-math>$1.47~\\\\mu \\\\text{M}$ </tex-math></inline-formula>\\n, respectively. The engineered sensor was validated for quantifying the concentrations of L-Tyr in human blood and serum samples, yielding satisfactory recovery and can be expanded in future to detect analytes simultaneous.\",\"PeriodicalId\":13264,\"journal\":{\"name\":\"IEEE Transactions on NanoBioscience\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-03-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"IEEE Transactions on NanoBioscience\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://ieeexplore.ieee.org/document/10476627/\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"IEEE Transactions on NanoBioscience","FirstCategoryId":"99","ListUrlMain":"https://ieeexplore.ieee.org/document/10476627/","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Electrochemical Synthesis of Molecularly Imprinted Polymers for L-Tyrosine Detection
L-Tyrosine (L-Tyr), a critical amino acid whose aberrant levels impact melanin and dopamine levels in human body while also increasing insulin resistance thereby increasing the risk of type 2 diabetes. The objective of this study was to detect the amount of L-Tyr in human fluids by tailored electrochemical synthesis of well adhered, homogenous and thin molecularly imprinted polymers (MIPs) by the electro-polymerization of pyrrole on glassy carbon electrode modified functionalized multi-walled carbon nanotubes. The key benefits of this procedure over previous imprinting techniques were the elimination of expensive materials like Au and tedious multi-step synthesis, for L-Tyr detection using a handheld potentiostat. The developed particles were characterized using Fourier Transform Infrared Spectroscopy, Scanning Electron Microscope, Chronoamperometry, and Cyclic Voltammetry. With strong reproducibility and stability, this optimized approach provides a rapid and effective method of preparing and sensing MIPs for the target analyte with a broad linear range of
$1~\mu \text{M}$
to
$2000~\mu \text{M}$
. The Limit of Detection and Limit of Quantification were
$0.4~\mu \text{M}$
and
$1.47~\mu \text{M}$
, respectively. The engineered sensor was validated for quantifying the concentrations of L-Tyr in human blood and serum samples, yielding satisfactory recovery and can be expanded in future to detect analytes simultaneous.
期刊介绍:
The IEEE Transactions on NanoBioscience reports on original, innovative and interdisciplinary work on all aspects of molecular systems, cellular systems, and tissues (including molecular electronics). Topics covered in the journal focus on a broad spectrum of aspects, both on foundations and on applications. Specifically, methods and techniques, experimental aspects, design and implementation, instrumentation and laboratory equipment, clinical aspects, hardware and software data acquisition and analysis and computer based modelling are covered (based on traditional or high performance computing - parallel computers or computer networks).