用于检测 L-酪氨酸的分子印迹聚合物的电化学合成。

IF 3.7 4区 生物学 Q1 BIOCHEMICAL RESEARCH METHODS
K. Ramya;K. S. Jaya Lakshmi;Khairunnisa Amreen;Sanket Goel
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引用次数: 0

摘要

L-酪氨酸(L-Tyr)是一种重要的氨基酸,其异常水平会影响人体内黑色素和多巴胺的水平,同时还会增加胰岛素抵抗,从而增加罹患 2 型糖尿病的风险。本研究的目的是通过在玻璃碳电极修饰的功能化多壁碳纳米管上对吡咯进行电聚合,定制电化学合成粘附性好、均匀且薄的分子印迹聚合物(MIPs),从而检测人体液中的 L-Tyr 含量。与以往的压印技术相比,该方法的主要优点是省去了金等昂贵材料和繁琐的多步合成过程,可使用手持式恒电位仪检测 L-Tyr。使用傅立叶变换红外光谱仪、扫描电子显微镜、计时电流计和循环伏安法对所开发的颗粒进行了表征。这种优化方法具有很强的重现性和稳定性,为制备和传感目标分析物的 MIPs 提供了一种快速有效的方法,其线性范围为 1 μM 至 2000 μM。检测限和定量限分别为 0.4 μM 和 1.47 μM。经验证,该工程传感器可定量检测人体血液和血清样本中的 L-Tyr 浓度,回收率令人满意,今后还可扩展到同时检测分析物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Electrochemical Synthesis of Molecularly Imprinted Polymers for L-Tyrosine Detection
L-Tyrosine (L-Tyr), a critical amino acid whose aberrant levels impact melanin and dopamine levels in human body while also increasing insulin resistance thereby increasing the risk of type 2 diabetes. The objective of this study was to detect the amount of L-Tyr in human fluids by tailored electrochemical synthesis of well adhered, homogenous and thin molecularly imprinted polymers (MIPs) by the electro-polymerization of pyrrole on glassy carbon electrode modified functionalized multi-walled carbon nanotubes. The key benefits of this procedure over previous imprinting techniques were the elimination of expensive materials like Au and tedious multi-step synthesis, for L-Tyr detection using a handheld potentiostat. The developed particles were characterized using Fourier Transform Infrared Spectroscopy, Scanning Electron Microscope, Chronoamperometry, and Cyclic Voltammetry. With strong reproducibility and stability, this optimized approach provides a rapid and effective method of preparing and sensing MIPs for the target analyte with a broad linear range of $1~\mu \text{M}$ to $2000~\mu \text{M}$ . The Limit of Detection and Limit of Quantification were $0.4~\mu \text{M}$ and $1.47~\mu \text{M}$ , respectively. The engineered sensor was validated for quantifying the concentrations of L-Tyr in human blood and serum samples, yielding satisfactory recovery and can be expanded in future to detect analytes simultaneous.
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来源期刊
IEEE Transactions on NanoBioscience
IEEE Transactions on NanoBioscience 工程技术-纳米科技
CiteScore
7.00
自引率
5.10%
发文量
197
审稿时长
>12 weeks
期刊介绍: The IEEE Transactions on NanoBioscience reports on original, innovative and interdisciplinary work on all aspects of molecular systems, cellular systems, and tissues (including molecular electronics). Topics covered in the journal focus on a broad spectrum of aspects, both on foundations and on applications. Specifically, methods and techniques, experimental aspects, design and implementation, instrumentation and laboratory equipment, clinical aspects, hardware and software data acquisition and analysis and computer based modelling are covered (based on traditional or high performance computing - parallel computers or computer networks).
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