Zhenlu Liu, Ben Cao, Qi Shao, Yanfen Zheng, Bin Wang, Shihua Zhou, Pan Zheng
{"title":"Family of Mutually Uncorrelated Codes for DNA Storage Address Design.","authors":"Zhenlu Liu, Ben Cao, Qi Shao, Yanfen Zheng, Bin Wang, Shihua Zhou, Pan Zheng","doi":"10.1109/TNB.2025.3530470","DOIUrl":"https://doi.org/10.1109/TNB.2025.3530470","url":null,"abstract":"<p><p>Deoxyribonucleic acid (DNA) has become an ideal medium for long-term storage and retrieval due to its extremely high storage density and long-term stability. But access efficiency is an existing bottleneck in DNA storage, especially the lack of high-quality random access address sequences. Therefore, in this paper, we report a series of approaches based on k-weakly mutually uncorrelated (k-WMU) codes to design the address sequence to improve the access efficiency of DNA storage. To address the problem of DNA sequences that are poorly scalable at the base level, we propose a 0-m-ruling coding scheme combined with k-WMU codes that can make address sequences avoid generating secondary structure with stem lengths ranging from 3 to 9. Based on the decoupled structure, We further extend the k-WMU codes with error correction function while satisfying combinatorial biological constraints. In order to investigate the performance of the designed address sequences for real-world applications, we perform simulation experiments based on thermodynamic properties and error correction capability as well as compared the minimum free energy (MFE), melting temperature (TM), and average decoding success rate (ADSR) with previous work. The results show that designed address sequences have a high MFE value and ADSR and a substantial reduction in TM-variance while satisfying the combinatorial biological constraints. As the quality of address sequences improves, this will help to achieve accurate random access as well as enhance the robustness of the DNA storage system.</p>","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"PP ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143541762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhen Cheng;Zhichao Zhang;Heng Liu;Dongliang Jing;Weihua Gong;Kaikai Chi
{"title":"Neural Network With Attention Mechanism for Abnormality Detection and Localization in Diffusive Molecular Communication","authors":"Zhen Cheng;Zhichao Zhang;Heng Liu;Dongliang Jing;Weihua Gong;Kaikai Chi","doi":"10.1109/TNB.2025.3527520","DOIUrl":"10.1109/TNB.2025.3527520","url":null,"abstract":"Diffusive molecular communication (DMC) is an emerging paradigm in nanotechnology, which provides biocompatibility and nanoscale communication for many promising applications, such as targeted drug delivery, environmental monitoring, etc. However, detecting and localizing abnormalities in most of these applications is challenging, such as identifying tumor cells within the body or detecting pollution in air or water. In this paper, we introduce a method for detecting and localizing abnormalities in three dimensional DMC system with multiple sensors, receivers and one fusion center by adopting Transformer-based model with attention mechanism. We make full use of the attention mechanism to capture the inter-symbol interference (ISI) to improve the accuracy of detection and localization. In addition, we simplify the model structure to significantly reduce the complexity of this model. Furthermore, two strategies that different types of molecules (DMT) and same type of molecules (SMT) are released by sensors are considered. The training dataset and testing dataset are generated under these two strategies. Simulation results show that the information about the abnormality detection and localization can be obtained at the same time based on the Transformer-based model under DMT and SMT. Especially, our model outperforms the Informer-based model, deep neural networks (DNN)-based model and log-likelihood ratio (LLR) method.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 2","pages":"257-267"},"PeriodicalIF":3.7,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143541773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oindrila Banik;Bansod Sneha Bharat;Anju R. Babu;Prasoon Kumar;Santosh Kumar;Earu Banoth
{"title":"Recycling Eggshell Waste Into Calcium Oxide Nanoparticles: A Sustainable Approach for Nanomaterial Synthesis and Potential Applications","authors":"Oindrila Banik;Bansod Sneha Bharat;Anju R. Babu;Prasoon Kumar;Santosh Kumar;Earu Banoth","doi":"10.1109/TNB.2025.3526975","DOIUrl":"10.1109/TNB.2025.3526975","url":null,"abstract":"Eggshell (ES) wastes have been ranked as the <inline-formula> <tex-math>$15^{text {th}}$ </tex-math></inline-formula> food industry pollution due to the ever-increasing regular consumption of primary dietary products, eggs. Management and treatment of tons of discarded eggshells produced daily on a global scale are realized to be a predicament, and an immediate solution must be advocated to address the pollution. This sets a tone for the recyclability of this biowaste in a myriad of fields, like nanotechnology, biomedical, and environmental pollution control. Calcium carbonate in the shells makes it a safe precursor for producing calcium oxide as a nanomaterial by the top-down approach – calcination. This paper highlights a facile way to procure waste eggshell-derived metal oxide nanoparticles with reproducibility and recyclability. Calcium Oxide Nanoparticles (CaO NPs) obtained at two different calcination temperatures for optimization and this was characterized by SEM, FTIR, XRD, DLS, and Zeta Potential analyzer. CaONPs are less-studied metal oxide nanoparticles but hold promising applications in different fields. Hence, there is a scope for further investigation on the non-toxic, non-hazardous CaO NPs obtained facilely – an effort to minimize and regulate food wastes.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 2","pages":"249-256"},"PeriodicalIF":3.7,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143541777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"IEEE Transactions on NanoBioscience Information for Authors","authors":"","doi":"10.1109/TNB.2024.3514239","DOIUrl":"https://doi.org/10.1109/TNB.2024.3514239","url":null,"abstract":"","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 1","pages":"C3-C3"},"PeriodicalIF":3.7,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=10820093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142912453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ZebraVas: A Non-Invasive Microvision System for Vascular Recognition and Blood Flow Monitoring of Zebrafish Larvae","authors":"Zhongyi Guo;Nana Ai;Wei Ge;Qingsong Xu","doi":"10.1109/TNB.2024.3520137","DOIUrl":"10.1109/TNB.2024.3520137","url":null,"abstract":"Zebrafish have emerged as a powerful model organism in cardiovascular disease research. Accurately identifying zebrafish blood vessels and evaluating blood flow velocity without injury has a wide range of biological applications. This paper presents the design and development of a non-invasive microvision system for vascular recognition and blood flow monitoring of zebrafish larvae. For the first time, a visual algorithm based on color thresholding and discrete Fourier transform filtering is proposed to determine the position of zebrafish dorsal cardinal vein vessels. Next, the blood flow velocity is determined based on the change rate of pixel values near the centroid point of the blood vessel recognition results. Then, an independent software system is developed based on the producer-consumer underlying framework. A user-friendly interface is specifically designed for biomedical workers, and a complete prototype system is built in combination with hardware devices. In addition, relevant experiments were conducted, and the results indicated that the system can effectively recognize the position of vessels and monitor blood flow velocity in zebrafish larvae under different anesthesia concentrations and developmental days. The heart rate information obtained based on blood flow velocity is consistent with the heart beating frequency. Moreover, the system has also been successfully applied to blood flow velocity monitoring under fluorescence conditions. In future work, this system will be applied in drug screening research for cardiovascular-related diseases of zebrafish larvae.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 2","pages":"225-233"},"PeriodicalIF":3.7,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143541726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Microtubule Deformation Modulates Intracellular Transport by Kinesin Differently Than Dynein","authors":"Syeda Rubaiya Nasrin;Tanjina Afrin;Arif Md. Rashedul Kabir;Daisuke Inoue;Takefumi Yamashita;Makoto Oura;Johtaro Yamamoto;Masataka Kinjo;Kazuki Sada;Akira Kakugo","doi":"10.1109/TNB.2024.3507021","DOIUrl":"10.1109/TNB.2024.3507021","url":null,"abstract":"Mechanical stress on cells is transmitted through many biological processes, for example, cell shape control, tissue patterning, and axonal homeostasis. Microtubules, a cytoskeletal component, presumably play a significant role in the mechanoregulation of cellular processes. We investigate motor protein-driven transport of quantum dots along mechanically deformed microtubules. We found that microtubule deformation significantly slowed kinesin-driven transport, whereas we previously reported dynein-driven transport was rather robust. Such dualistic modulation of transportation dynamics of the motor proteins by microtubule deformation can be attributed to the altered affinity of the motor proteins for buckled microtubules. Our results may form the basis for understanding microtubules’ role in regulating cellular processes in a mechanically adverse environment through its detection ability and response to mechanical stress.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 2","pages":"218-224"},"PeriodicalIF":3.7,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Investigating Physical Layer Security in Molecular Communication Networks","authors":"Fatemeh Sadat Saeidi;Naghmeh Sadat Moayedian","doi":"10.1109/TNB.2024.3504540","DOIUrl":"10.1109/TNB.2024.3504540","url":null,"abstract":"In molecular communication networks, understanding the security level allows us to assess the quality of information transmitted accurately. The presence of unintended nodes in these networks is one of the factors compromising the security of information. This paper considers the simultaneous presence of a jammer and an eavesdropper as unintended nodes. This existence of unintended nodes prompts us to explore methods for assessing the security of a proposed system. Physical layer approaches can be regarded as one of the most efficient methods for assessing security in molecular communication networks. In this paper, we have utilized these approaches instead of the conventional cryptographic methods. At this layer, we have used several metrics to evaluate the security of our system; secrecy capacity (SC), the average probability of error (APOE), and comprehensive secure distance (CSD). By using SC, we also employed other approaches to improve security, such as changing the time interval, jamming molecules, and varying the distance between the transmitter and the receiver. As the last step, Monte Carlo simulation is used to verify the results obtained through analytical analysis.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 2","pages":"208-217"},"PeriodicalIF":3.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Electrospun Stannic Oxide Nanofiber Thin-Film Based Sensing Device for Monitoring Functional Behaviors of Adherent Mammalian Cells","authors":"Uvanesh Kasiviswanathan;Chandan Kumar;Ajay Kumar Sahi;Amit Kumar;Satyabrata Jit;Neeraj Sharma;Sanjeev Kumar Mahto","doi":"10.1109/TNB.2024.3489353","DOIUrl":"10.1109/TNB.2024.3489353","url":null,"abstract":"This study presents a biosensor utilizing electrospun SnO2 nanofiber films for real-time monitoring of C2C12 cells. The biosensor demonstrates sensitivity towards cellular behaviors, including adhesion, proliferation, and detachment. Alterations in semi-circle and dielectric properties are validated through Nyquist plot and an EEC model, highlighting the biosensor’s potential for analyzing cellular dynamics.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 1","pages":"120-126"},"PeriodicalIF":3.7,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"“Galaxy” Encoding: Toward High Storage Density and Low Cost","authors":"Xuncai Zhang;Yunfei Lu","doi":"10.1109/TNB.2024.3481504","DOIUrl":"10.1109/TNB.2024.3481504","url":null,"abstract":"DNA is considered one of the most attractive storage media because of its excellent reliability and durability. Early encoding schemes lacked flexibility and scalability. To address these limitations, we propose a combination of static mapping and dynamic encoding, named “Galaxy” encoding. This scheme uses both the “dual-rule interleaving” algorithm and the “twelve-element Huffman rotational encoding” algorithm. We tested it with “Shakespeare Sonnets” and other files, achieving an encoding information density of approximately 2.563 bits/nt. Additionally, the inclusion of Reed–Solomon error-correcting codes can correct nearly 5% of the errors. Our simulations show that it supports various file types (.gz, .tar, .exe, etc.). We also analyzed the cost and fault tolerance of “Galaxy” encoding, demonstrating its high coding efficiency and ability to fully recover original information while effectively reducing the costs of DNA synthesis and sequencing.","PeriodicalId":13264,"journal":{"name":"IEEE Transactions on NanoBioscience","volume":"24 2","pages":"200-207"},"PeriodicalIF":3.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}