Horticultural Plant Journal最新文献

{"title":"Anatomical and dual transcriptomic analysis reveals the interaction of “Candidatus Liberibacter asiaticus” and citrus host in new shoots at different growth stage","authors":"Jiaming Li, Jinghan Chen, Wenxia Huang, Xiaohong Chen, Cuixiao Li, Meirong Xu, Xiaoling Deng, Zheng Zheng","doi":"10.1016/j.hpj.2025.04.003","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.04.003","url":null,"abstract":"“<ce:italic>Candidatus</ce:italic> Liberibacter asiaticus” (CLas), the causal agent of citrus Huanglongbing (HLB), poses a significant threat to citrus production worldwide. New shoots play a crucial role in the HLB pathosystem, serving as key sites for CLas acquisition and transmission by insect vector. However, CLas-host interactions in newly developing shoots remain poorly understood. This study investigated CLas population dynamic, anatomical change and dual transcriptomic profiles in new shoots at different growth stage after pruning two-years-old CLas-infected citrus plants. CLas population increased rapidly within 2 weeks after pruning (WAP), peaked at 4 WAP and subsequently declined between 6 WAP and 8 WAP. CLas infection inhibited the shoots growth and induced severe phloem collapse, particularly at 8 WAP. Transcriptomic analyses revealed that CLas genes involved in transcription/translation, lipid transport and metabolism, energy production, and resilience to host immune response were highly expressed at 4 WAP and 8 WAP, indicating active bacterial adaptation. In citrus, CLas infection primary affected metabolism, hormone signal transduction, cell wall metabolism, and transcription factors, particularly trihelix and bHLH families. The activation of citrus genes related to α-linolenic acid, WRKY and MYB transcription factors, pathogenesis-related proteins, and Ca<ce:sup loc=\"post\">2+</ce:sup>-mediated signaling suggested potential defense response against in new shoots. These finding provide a comprehensive transcriptome landscape of CLas and its host in developing shoots, offering new insights into CLas-host interactions and potential defense mechanisms in citrus.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"16 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative responsive patterns of Opisthopappus taihangensis and Opisthopappus longilobus under salt stress by weighted gene co-expression network analysis (WGCNA) and genome-wide association studies (GWAS)","authors":"Yuexin Shen, Yimeng Zhang, Mian Han, Ting Gao, Xiaojuan Zhou, Qi Wu, Tingyu Wang, Haoyuan Dan, Li Liu, Min Chai, Yiling Wang","doi":"10.1016/j.hpj.2025.04.004","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.04.004","url":null,"abstract":"The unique and challenging environment of the cliffs gives <ce:italic>Opisthopapus</ce:italic> genus (<ce:italic>Opisthopappus taihangensis</ce:italic> and <ce:italic>Opisthopappus longilobus</ce:italic>) a strong tolerance to harsh climatic conditions. Under salt stress, the physiological indexes, superoxide dismutase (SOD), soluble protein (SP), chlorophyll (Chl), malondialdehyde (MDA), peroxidase (POD), and catalase (CAT), generally increased with the treatment levels. Through weighted gene co-expression network analysis (WGCNA), MEdarkmagenta module in <ce:italic>O. taihangensis</ce:italic> (182 genes) and MEdarkgreen module in <ce:italic>O. longilobus</ce:italic> (281 genes) were screened, which significantly related with SOD, CAT, SP, and POD. With genome-wide association studies (GWAS), it found that abundant SNPs was significantly correlated with the SOD index. Among WGCNA and GWAS, there was eleven common differentially expressed genes (c-DEGs) in <ce:italic>O. taihangensis</ce:italic> and seventeen c-DEGs in <ce:italic>O. longilobus</ce:italic>, respectively. For <ce:italic>O. taihangensis,</ce:italic> it responded to the stress primarily through the proteasome and lipid metabolism pathways involved in <ce:italic>PSMB2</ce:italic>, <ce:italic>PSMA4</ce:italic>, and <ce:italic>PLD1_2</ce:italic> genes. In contrast, <ce:italic>O. longilobus</ce:italic> activated the oxidative phosphorylation pathway and then influenced the amino acid biosynthesis, terpenoid biosynthesis, and signaling transduction pathways (e.g., GA, JA, and MAPK) mainly involved <ce:italic>MYC2</ce:italic>, <ce:italic>GID1</ce:italic>, <ce:italic>CYP82G1</ce:italic>, <ce:italic>TYRAAT</ce:italic>, and <ce:italic>MAPKKK17_18</ce:italic> genes. Compared with <ce:italic>O. taihangensis, O. longilobus</ce:italic> activated additional genes through more pathways to respond to salt stress. According to May-Wigner theory, the simple gene regulatory network was more stable than the complex. Thus, it suggested that <ce:italic>O. taihangensis</ce:italic> had superior tolerance and adaptation than <ce:italic>O. longilobus.</ce:italic> The results initially explored the potential responsive mechanism of two species under salt stress. These would establish the foundation for further investigations of the <ce:italic>Opisthopapus</ce:italic> tolerances, provide clues for the study of other cliff plants in the Taihang Mountains, and lay lights for the performing of Asteraceae cross-breeding.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"45 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A natural variant in MdNAC25 promoter contributes to fruit harvest date divergence in apple","authors":"Zhiyong Wu, Zhe Zhou, Yarong Wang, Chunyan Wang, Zhenli Yan, Qiming Gao, Zhenzhen Liu, Yaru Zhang, Xiangpeng Meng, Yiru Yang, Hussain Ahmed, Md Marufur Rahman, Hengtao Zhang, Jia-Long Yao","doi":"10.1016/j.hpj.2025.05.002","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.05.002","url":null,"abstract":"Fruit ripening is a quantitative trait governed by multiple genes, influencing fruit quality and commercial values. However, the genetic mechanisms underlying this process remain poorly understood. Here, we elucidate the role of <ce:italic>MdNAC25</ce:italic> in regulating apple fruit ripening. We identified allele-specific expression (ASE) of <ce:italic>MdNAC25</ce:italic> in various apple cultivars, driven by a 4-bp deletion in its promoter. This deletion created a key regulatory element, ACS2-TAAAATAT, which enhanced <ce:italic>MdNAC25</ce:italic> promoter activity in tobacco leaf promoter-reporter assays. Additionally, a long non-coding RNA (<ce:italic>lncRNA-nac25</ce:italic>) located upstream of <ce:italic>MdNAC25</ce:italic> also exhibited ASE and was positively correlated with <ce:italic>MdNAC25</ce:italic> expression. The ACS2-TAAAATAT element, positioned between <ce:italic>MdNAC25</ce:italic> and <ce:italic>lncRNA-nac25,</ce:italic> functioned as a bidirectional enhancer, regulating both genes. Overexpression and silencing experiments demonstrated that <ce:italic>lncRNA-nac25</ce:italic> enhanced <ce:italic>MdNAC25</ce:italic> expression. Genetic association studies in 154 apple accessions linked the <ce:italic>MdNAC25</ce:italic> allele with the 4-bp deletion to earlier fruit ripening. This allele was significantly more frequent in cultivated apples than in wild species, indicating artificial selection. Furthermore, overexpression of <ce:italic>MdNAC25</ce:italic> in non-ripening (nor) mutant tomatoes restored ripening, reinforcing its functional significance. Comparative genomic analysis revealed conservation of the ACS2-TAAAATAT element across Rosaceae fruit trees, including peach, pear, plum, and apricot, underscoring <ce:italic>MdNAC25</ce:italic> as a key regulator of fruit ripening across species.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"36 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144305100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potato StCERK1 is a key regulator of plant immunity against multiple pathogens","authors":"Lijuan Cao, Mingchuan Pan, Langhua Yu, Chuntao Lv, Dandan Zhao, Wei Wu, Futing Li, Ju Ai, Dongli Gao, Sanwen Huang, Yi Shang, Xuming Luo","doi":"10.1016/j.hpj.2025.03.006","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.03.006","url":null,"abstract":"Potato (<ce:italic>Solanum tuberosum</ce:italic> L.), the world's third-largest crop, faces significant yield losses due to susceptibility to diverse plant pathogens, impacting global food security. Receptor-like kinases (RLKs) activate plant immunity by recognizing damage- and pathogen-associated molecular patterns (DAMPs and PAMPs). Among these, chitin elicitor receptor kinase 1 (CERK1) is essential for detecting chitin and confers resistance to various pathogens in <ce:italic>Arabidopsis thaliana</ce:italic>, <ce:italic>Oryza sativa</ce:italic>, and <ce:italic>Solanum lycopersicum</ce:italic>. However, generating homozygous mutants in potato is challenging, leaving functions of CERK1 in potato (StCERK1) unexplored. This study identified StCERK1 in potato and applied a tRNA scaffolded gRNA editing strategy with four gRNAs to edit the <ce:italic>StCERK1</ce:italic> gene in tetraploid potato, generating homozygous mutants with high efficiency. We confirmed StCERK1 as a functional kinase and found that it was essential for chitin signal response in potato. It is likely involved in regulating potato immunity through the flavonoid biosynthesis pathway. Furthermore, phenotypic analysis revealed that <ce:italic>stcerk1</ce:italic> mutants exhibit increased susceptibility to <ce:italic>Phytophthora infestans, Alternaria solani,</ce:italic> and <ce:italic>Ralstonia solanacearum</ce:italic> compared to the wild type. Notably, StCERK1 was also implicated in tuber disease resistance. These findings highlight StCERK1 as a key regulator of potato immunity against multiple pathogens, suggesting potential strategies for broad-spectrum crop resistance against diseases in agricultural production through the utilization of plant immune receptors.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"625 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144305101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Classification of diverse pear genetic resources using a combination of MIG-seq and whole-genome sequencing","authors":"Sogo Nishio, Norio Takada, Yukie Takeuchi, Shuri Kato, Hiroyuki Iketani","doi":"10.1016/j.hpj.2025.03.003","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.03.003","url":null,"abstract":"Pears (<ce:italic>Pyrus</ce:italic> spp.), which are distributed across temperate regions of Eurasia, include several economically important species. Pear species and cultivar classification is always controversial, because hybridization occurs easily, due to a low reproductive barrier. To reveal the genetic diversity and genetic relationships among pear genetic resources, we applied multiplexed inter-simple sequence repeat genotyping by sequencing (MIG-seq) to accessions registered in the National Agriculture and Food Research Organization (NARO) Genebank, including several introduced from abroad and the wild individuals collected in Japan. In addition, whole-genome sequencing data from a public database were combined with MIG-seq data to improve the reliability of species and group classifications. Out of the 1018 accessions genotyped, we identified 97 synonym groups consisting of 267 accessions. After eliminating all but one accession from each synonym group, we identified some mislabeled and misclassified accessions by means of population structure analysis. The genetic clusters estimated by our population structure analyses revealed that our materials and criteria for pear group and species classification were similar to those used for the public database. Our analyses clarified that (1) <ce:italic>P. ussuriensis</ce:italic> var. <ce:italic>aromatica</ce:italic> arose from hybridization of Japanese pear (<ce:italic>P</ce:italic>. <ce:italic>pyrifolia</ce:italic>) and <ce:italic>P. ussuriensis</ce:italic> var. <ce:italic>hondoensis</ce:italic>, (2) the genetic composition of Japanese pear originated mainly from Chinese pear, whereas ancestral compositions from <ce:italic>P. ussuriensis</ce:italic> var. <ce:italic>hondoensis</ce:italic> were very limited in Japanese pear, and (3) <ce:italic>P. pashia</ce:italic> in Nepal and <ce:italic>P. dimorphophylla</ce:italic>, included in Asian pea pear groups, contain unique ancestral compositions that are genetically distinct from those of other Asian pea pear groups.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"10 8 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic analysis of leaf apoplast reveals that a jasmonate-regulated CHIA participates in the response to cold and drought stress in jojoba","authors":"Lamei Zheng, Bojing Li, Qi Liu, James Edward Richardson, Yijun Zhou, Fei Gao","doi":"10.1016/j.hpj.2025.03.005","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.03.005","url":null,"abstract":"Jojoba is a woody oil crop of economic importance. However, it is sensitive to low temperatures, which hinders its cultivation in temperate arid regions. The apoplast is a cellular component outside the plasma membrane of plant cells and is widely involved in the response of plants to environmental stress. Here, we used the infiltration-centrifugation method to extract apoplast fluid from jojoba leaves and analyzed changes in the apoplast proteome after cold acclimation using quantitative proteomics. In total, 751 apoplast proteins were identified in jojoba, and the abundance of 200 proteins showed significant changes after cold acclimation. These proteins were primarily involved in defense, cell wall modification, carbohydrate metabolism, and redox balance. We also investigated the function and regulation of a cold acclimation-responsive class III chitinase <ce:italic>ScCHIA</ce:italic>. The results showed that the overexpression of <ce:italic>ScCHIA</ce:italic> enhanced the tolerance of <ce:italic>Arabidopsis</ce:italic>, yeast, and jojoba to low temperature and osmotic stress. Under cold stress, <ce:italic>ScCHIA</ce:italic>-overexpressing <ce:italic>Arabidopsis</ce:italic> accumulated more osmolytes, activated antioxidant enzymes, and reduced stomatal aperture size, which may contribute to enhanced tolerance to cold stress. <ce:italic>ScCHIA</ce:italic> was induced by methyl jasmonate (MeJA) application, and electrophoretic mobility shift assay (EMSA), yeast one-hybrid (Y1H), and luciferase activity assays demonstrated that an E-box <ce:italic>cis</ce:italic>-acting element on the <ce:italic>ScCHIA</ce:italic> gene promoter mediated regulation of <ce:italic>ScCHIA</ce:italic> by MeJA signaling, indicating that elevated levels of MeJA caused by cold acclimation may promote the expression of <ce:italic>ScCHIA</ce:italic>, thereby enhancing the cold tolerance of jojoba. Our research highlights the important role of the apoplast in plant response to low temperature stress and improves the understanding of the functions and regulatory mechanisms of plant chitinases in abiotic stress response.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"34 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MdMYB93 activates MdHCT6 expression via transcriptional regulation to enhance chlorogenic acid biosynthesis in apples","authors":"Yuying Xie, Wenyan Wang, Chukun Wang, Ze Chen, Yu Wang, Rong Zhang, Ruigang Wu, Quan Sun, Dagang Hu","doi":"10.1016/j.hpj.2025.05.001","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.05.001","url":null,"abstract":"Chlorogenic acid (CGA), a potent antioxidant with antimicrobial, antiviral, and metabolic regulatory properties, plays multifunctional roles in apple fruit by enhancing postharvest quality, extending shelf life through oxidative stress reduction, and inhibiting enzymatic browning to preserve color, flavor, and nutritional integrity. Despite the established role of hydroxycinnamoyl-CoA transferase (HCT) as a rate-limiting enzyme in CGA biosynthesis, the specific <ce:italic>HCT</ce:italic> gene responsible for this process and its regulatory mechanisms remain elusive. To address this knowledge gap, we systematically investigated CGA accumulation dynamics during apple storage and functionally characterized <ce:italic>MdHCT6</ce:italic>, a candidate gene within the HCT family. We found that the chlorogenic acid content in apple fruit increased significantly during postharvest storage compared with the initial storage. Transcriptome analysis showed that the expression level of <ce:italic>MdHCT6</ce:italic> was significantly higher than that of other HCT homologues, which was consistent with the reverse transcription quantitative PCR (RT-qPCR) results. <ce:italic>In vitro</ce:italic> enzymatic assays demonstrated that <ce:italic>MdHCT6</ce:italic> catalyzes the synthesis of chlorogenic acid using shikimic acid and quinic acid as precursors, while genetic evidence confirmed its role as a key positive regulator of chlorogenic acid accumulation in apples. Furthermore, we identified the transcription factor MdMYB93 as a direct upstream activator of <ce:italic>MdHCT6</ce:italic>, establishing a regulatory cascade that governs CGA production. This work not only deciphers the molecular hierarchy of CGA biosynthesis in apples but also provides actionable targets for genetic improvement of antioxidant capacity and postharvest resilience in apple germplasm.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"4 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144237346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZeEIL1–ZeERF113–ZeMC2 cascade enhances colored calla lily resistance to soft rot through the ethylene signaling pathway","authors":"Rongxin Gou, Yi Wang, Tuo Yang, Zhen Zeng, Di Wang, Yin Jiang, Guojun Zhang, Defeng Chen, Zunzheng Wei","doi":"10.1016/j.hpj.2025.03.004","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.03.004","url":null,"abstract":"Soft rot caused by <ce:italic>Pectobacterium carotovorum</ce:italic> poses a major threat to the cultivation of colored calla lily (<ce:italic>Zantedeschia elliottiana</ce:italic>). This study identified ZeERF113 through transcriptome analysis and weighted gene coexpression network analysis (WGCNA), revealing its close association with the accumulation of 1-aminocyclopropane-1-carboxylate (ACC) in colored calla lily after soft rot infection. The overexpression of <ce:italic>ZeERF113</ce:italic> in <ce:italic>Nicotiana tabacum</ce:italic> significantly increased resistance to the pathogen, as evidenced by reduced lesion size, lower malondialdehyde (MDA) content, and elevated levels of various antioxidant enzymes. In colored calla lily, transient overexpression of <ce:italic>ZeERF113</ce:italic> enhanced resistance to <ce:italic>P. carotovorum</ce:italic>, whereas silencing <ce:italic>ZeERF113</ce:italic> weakened this resistance. Through yeast one-hybrid (Y1H) assays, dual-luciferase reporter assays, electrophoretic mobility shift assays (EMSAs), and biolayer interferometry (BLI) assays, we confirmed that ZeERF113 binds to and activates the promoter of <ce:italic>ZeMC2</ce:italic>. Transient overexpression of <ce:italic>ZeMC2</ce:italic> in colored calla lily significantly enhanced resistance to <ce:italic>P. carotovorum</ce:italic>, while silencing <ce:italic>ZeMC2</ce:italic> compromised this resistance. This study further revealed that ZeEIL1 responds to ethylene (ETH) signaling and subsequently activates the expression of <ce:italic>ZeERF113</ce:italic>, as demonstrated by Y1H assays, dual-luciferase reporter assays, EMSAs, and BLI assays. This discovery highlights the critical role of the ETH-regulated ZeEIL1–ZeERF113–ZeMC2 cascade in enhancing resistance to soft rot in colored calla lily, providing new insights into its disease resistance mechanism.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"58 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanotechnology in plant tissue culture: A review","authors":"Sabreena Jan, Nayeema Jan, Seema Singh, Muhammad Ashraf Shah, Irshad Ahmad Bhat","doi":"10.1016/j.hpj.2025.02.019","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.02.019","url":null,"abstract":"Plant tissue culture represents an advanced biotechnological technique for propagating and conserving threatened plant species efficiently. This method enables the rapid production of genetically identical plants under controlled sterile laboratory conditions (<ce:italic>in vitro</ce:italic>). Its applications span forestry, horticulture, and, crucially, plant breeding. Nanoparticles have emerged as innovative tools to address limitations in conventional plant tissue culture, offering diverse functionalities based on their unique physicochemical properties. This review focuses on the utilization of nanotechnology in enhancing various aspects of plant tissue culture. Nanoparticles, such as silver and zinc oxide, have demonstrated significant roles as antimicrobial agents and anti-browning agents. They also serve as elicitors, stimulating callus proliferation, root elongation, rapid shoot formation, and the enhanced production of bioactive compounds on a large scale. Furthermore, nanoparticles contribute to mitigating oxidative stress within cells, thereby promoting increased callus formation, elongated roots, and elevated production of secondary metabolites. Their influence extends to somaclonal variation and genetic transformation processes within plant tissue culture. These contributions collectively underscore the potential of nanoparticles to foster more efficient, sustainable, and scalable biotechnological solutions in <ce:italic>in vitro</ce:italic> culture. The implications extend to reducing resource dependency and mitigating environmental impacts, positioning nanotechnology as a transformative approach in sustainable plant biotechnology.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"37 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of an efficient Agrobacterium rhizogenes-mediated hairy root transformation method for subtropical fruit trees","authors":"Mao Yin, Yonghua Jiang, Yingjie Wen, Fachao Shi, Hua Huang, Qian Yan, Hailun Liu","doi":"10.1016/j.hpj.2025.04.001","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.04.001","url":null,"abstract":"","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"28 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}