Horticultural Plant Journal最新文献

{"title":"An optimized protoplast regeneration and DNA-free genome-editing system for Chinese cabbage (Brassica rapa L. ssp. pekinensis)","authors":"Solhee Bae, Jeung-Sul Han, Beum-Chang Kang","doi":"10.1016/j.hpj.2026.04.003","DOIUrl":"https://doi.org/10.1016/j.hpj.2026.04.003","url":null,"abstract":"","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"7 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148312993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional and post-translational networks mediated by MYC2 in governing fruit and vegetable physiology","authors":"Xiaodong Fu, Fujun Li, Yanan Li, Xiaoan Li, Xinhua Zhang, Zienab F.R. Ahmed","doi":"10.1016/j.hpj.2026.03.013","DOIUrl":"https://doi.org/10.1016/j.hpj.2026.03.013","url":null,"abstract":"MYC2 acts as a pivotal transcription factor in the regulation of growth and stress responses in fruits and vegetables (F&amp;Vs), helping them adapt to adverse conditions. The changes in MYC2 protein activity and its degradation are essential for maintaining the quality of F&amp;Vs. <ce:italic>MYC2</ce:italic> expression relies on epigenetic modifications and noncoding RNA regulation, whereas its protein activity is closely associated with post-translational modifications and protein–protein interactions. Additionally, hormonal signaling pathways are critical for MYC2 to respond to stress signals and mediate gene expression. In this review, we analyzed the structure of MYC2 and its role in quality formation and deterioration processes during growth and stress responses in F&amp;Vs. Subsequently, we discuss the transcription of <ce:italic>MYC2</ce:italic> genes, focusing on their responsiveness to stresses and phytohormones, their mutual regulation, and elaborate on their involvement in the regulatory mechanisms of ncRNAs. Finally, we summarized recent advances in post-translational modifications and interactions with unmodified proteins in MYC2-mediated regulatory mechanisms. Additionally, we provide perspectives on the current research status of MYC2 in F&amp;V epigenetics. In this review, we aim to provide new insights into deciphering the physiological regulatory network of MYC2 in F&amp;Vs.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"76 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148312991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient Agrobacterium rhizogenes-mediated genetic transformation of Lycium barbarum and plant regeneration system","authors":"Mingyue Xu, Wenbin Su, Kun Zhao, Guibin Wang, Shuo Liu, Rui Feng, Ali Movahedi, Rui Zheng, Tingting Dai, Liming Yang","doi":"10.1016/j.hpj.2025.12.019","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.12.019","url":null,"abstract":"","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"21 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148312992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adapting breeding goals to greenhouse technological contexts for sustainable vegetable production","authors":"Dimitrios Fanourakis, Georgios Tsaniklidis, Theodora Makraki, Theodora Ntanasi, Evangelos Giannothanasis, Vasileios Papasotiropoulos, Jaime Prohens, Georgia Ntatsi","doi":"10.1016/j.hpj.2026.03.012","DOIUrl":"https://doi.org/10.1016/j.hpj.2026.03.012","url":null,"abstract":"Protected cultivation performs a central undertaking in safeguarding year-round vegetable production, providing controlled environments which shelter crops against climatic extremes. Nevertheless, greenhouse systems diverge extensively in technological complexity, from low-tech passive tunnels to fully automated, high-input structures. This review integrates contemporary knowledge on how tomato, cucumber, and sweet pepper breeding agendas are being customized to the specific demands of greenhouse technological level. In low-tech systems, prominence is positioned on early maturity, abiotic stress tolerance, and disease resistance under minimal input environments. Mid-tech greenhouses demand cultivars with enhanced shelf life, grafting compatibility, and steady performance under semi-controlled conditions. High-tech systems necessitate ideotypes optimized for artificial lighting, CO<ce:inf loc=\"post\">2</ce:inf> enrichment, soilless media, and intensive production cycles. Crucial holistic issues such as genotype by environment by management interactions, genomic selection, gene editing, participatory breeding, and sustainability-oriented traits are considered. The review underscores the imperative necessity for climate-resilient, resource-efficient cultivars and emphasizes the significance of cooperation between breeders, cultivators, and technology developers. By aligning breeding routes with greenhouse typologies and environmental restraints, the horticultural industry can evolve on the path to a more resilient, productive, and sustainable future.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"55 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148288311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precision breeding of cucumber: CRISPR-edited loci for trait improvement","authors":"Jiaxi Li, Mengyao Kong, Yuying Shi, Zhiwei Qin, Ming Xin","doi":"10.1016/j.hpj.2026.03.011","DOIUrl":"https://doi.org/10.1016/j.hpj.2026.03.011","url":null,"abstract":"Cucumber (<ce:italic>Cucumis sativus</ce:italic> L.) is a globally important vegetable crop. However, conventional breeding for trait improvement is limited by low efficiency and long breeding cycle duration. Over the past five years, CRISPR-based genome editing has rapidly advanced cucumber research, providing a precise tool for functional gene analysis and targeted improvement of agronomic traits. This review summarizes the recent strategies and representative applications of CRISPR technology in cucumber, focusing on plant architecture, stress tolerance, sex differentiation, parthenocarpy, and fruit development. The potential of CRISPR-edited loci for precision breeding in cucumber is further evaluated, along with the current technical constraints and challenges in trait integration. Finally, future perspectives are discussed, emphasizing the optimization of editing systems for multi-gene manipulation and systematic trait evaluation to accelerate the translation from gene discovery to breeding applications.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"72 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148288331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CsMYB75–CsTT8–CsTTG1 protein complex activates CsANS3 for anthocyanin biosynthesis in purple-leaf tea plants","authors":"Li Zhang, Hui Xie, Yue Hu, Hui Wang, Linlin Xu, Yongning Tao, Lingling Tao, Hongrong Chen, Xueming Ma, Honglian Gu, Deyu Xie, Jianbing Hu, Shengrui Liu, Junyan Zhu, Kun Dong, Chaoling Wei","doi":"10.1016/j.hpj.2026.04.002","DOIUrl":"https://doi.org/10.1016/j.hpj.2026.04.002","url":null,"abstract":"","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"54 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148288333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PrNAC72 activates PrDXS2 to regulate monoterpene biosynthesis in Paeonia rockii","authors":"Yue Zhang, Hui Zhi, Yingan Niu, Liuqing Qu, XiaoTing Feng, Dehu Su, Jianrang Luo, Yanlong Zhang","doi":"10.1016/j.hpj.2026.01.007","DOIUrl":"https://doi.org/10.1016/j.hpj.2026.01.007","url":null,"abstract":"<ce:italic>Paeonia rockii</ce:italic> is a woody ornamental plant of high horticultural value that is widely recognized for its distinctive terpene-rich floral aroma. However, the molecular regulation of terpene production in this species remains largely unclear. Here, dynamic volatile analysis throughout five flower development stages confirmed that monoterpenes are the dominant fragrance components. A combined transcriptomic approach using the Illumina RNA-seq and PacBio Iso-Seq platforms identified 7254 differentially expressed genes (DEGs), 147 of which were associated with terpenoid metabolic pathways. Notably, 16 DEGs were annotated as isoforms of 1-deoxy-<ce:small-caps>d</ce:small-caps>-xylulose 5-phosphate synthase (DXS), a rate-limiting enzyme in the methylerythritol phosphate (MEP) pathway. Among them, <ce:italic>PrDXS1</ce:italic> and <ce:italic>PrDXS2</ce:italic> exhibited petal-specific expression trends closely aligned with volatile monoterpene release, and both were localized to plastids. Functional assays, including transient overexpression and virus-induced gene silencing (VIGS), revealed that these genes positively influenced monoterpene accumulation. In addition, the NAC transcription factor PrNAC72 directly regulated <ce:italic>PrDXS2</ce:italic> expression, as validated by yeast one-hybrid, dual-luciferase, and GUS reporter experiments. The overexpression of <ce:italic>PrNAC72</ce:italic> in heterologous systems confirmed the role of <ce:italic>PrNAC72</ce:italic> in promoting monoterpene biosynthesis. These results reveal a novel regulatory pathway involving PrNAC72 and PrDXS2 in floral scent biosynthesis and offer promising molecular targets for enhancing aroma traits in tree peony breeding.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"26 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148288217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MdSPL6 regulates the accumulation of anthocyanins in apple leaves induced by low temperature by activating the promoters of anthocyanin-related genes","authors":"Mengjiao Zhang, Xiaoqing Zhou, Zhiying Wang, Yuncong Yao, Ji Tian, Jie Zhang","doi":"10.1016/j.hpj.2025.12.018","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.12.018","url":null,"abstract":"Anthocyanin accumulation in apple leaves is tightly regulated by environmental and hormonal signals. However, the mechanisms underlying cold perception in apple leaves remain poorly understood. These mechanisms mediate the crosstalk between low temperature and hormonal signals, which together regulate the anthocyanin biosynthetic pathway. In this study, we used apple (<ce:italic>Malus domestica</ce:italic>) cultivars ‘Royalty’, ‘Gala’, and ‘Orin’ as experimental materials. Through transcriptional expression analysis, downstream target gene screening, genetic functional validation and other molecular approaches, we aimed to dissect the molecular mechanism underlying the synergistic regulation of anthocyanin accumulation in apple leaves by low temperature and hormone signaling pathways. Here, we demonstrate that the transcription factor MdSPL6 plays a pivotal role in promoting anthocyanin biosynthesis in response to low–temperature and hormonal signaling. Expression analysis in <ce:italic>Malus domestica</ce:italic> ‘Royalty’ revealed that <ce:italic>MdSPL6</ce:italic> is strongly induced in both leaves and calli under low temperature, 16 °C, accompanied by increased anthocyanin accumulation. Hormonal treatments revealed that methyl jasmonate (MeJA) significantly upregulates <ce:italic>MdSPL6</ce:italic> expression and anthocyanin levels, with a synergistic effect observed under combined MeJA and low–temperature conditions. DNA affinity purification sequencing (DAP–seq) identified MdSPL6 target genes enriched in metabolic pathways including flavonoid biosynthesis. MdSPL6 directly binds and activates the promoters of flavonoid biosynthesis genes, as confirmed by yeast one–hybrid and dual–luciferase assays. Both transient expression in apple leaves and transformation in apple calli showed that MdSPL6 enhances red pigmentation and upregulates anthocyanin biosynthetic genes under low–temperature stress. Furthermore, miR156a may act as a negative regulator of <ce:italic>MdSPL6</ce:italic> and thereby participate in low–temperature–induced anthocyanin accumulation. Together, our findings suggest that <ce:italic>MdSPL6</ce:italic> acts as a central integrator of low temperature and hormonal signals to activate anthocyanin biosynthesis in apple leaves, providing new insights into the environmental regulation of pigmentation in woody plants.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"126 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148287489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smart horticulture era: advances in hyperspectral imaging for intelligent non-destructive quality assessment of fruits and vegetables","authors":"Demin Zhu, Yanfa Huang, Yao Zhang, Weiguo Yi, Yu Tian, Yanzhe Zhu, Yune Cao, Longguo Wu","doi":"10.1016/j.hpj.2026.01.006","DOIUrl":"https://doi.org/10.1016/j.hpj.2026.01.006","url":null,"abstract":"The rising consumer demand for safe, high-quality produce has spurred interest in efficient, non-destructive testing technologies. Hyperspectral imaging (HSI), integrating spectroscopy and imaging techniques, offers significant potential for rapid, precise, and non-destructive quality assessment of fruits and vegetables. HSI effectively predicts internal quality parameters, achieving high accuracy in firmness and SSC assessments for various produce, utilizing advanced machine learning and deep learning algorithms. It significantly improves the accuracy of maturity classification and external defect detection compared to traditional methods. Notably, HSI shows great promise for rapid pesticide residue detection. Nevertheless, HSI faces challenges such as slow data processing, high dimensionality, limited model generalization, and substantial equipment costs. Future research should emphasize enhancing algorithm efficiency, improving hardware affordability, and expanding applicability across diverse produce types to facilitate broader industry adoption.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"72 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148287488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated metabolomic and transcriptomic profiling provide insights into the biosynthetic mechanism of flavonoids in the plumule of Asian lotus (Nelumbo nucifera)","authors":"Heyun Song, Jia Xin, Heng Sun, Dong Yang, Xianbao Deng, Juan Liu, Mei Yang","doi":"10.1016/j.hpj.2026.03.009","DOIUrl":"https://doi.org/10.1016/j.hpj.2026.03.009","url":null,"abstract":"The lotus plumule is a pharmacologically important tissue rich in flavonoids; yet the molecular mechanisms underlying their biosynthesis remain poorly understood. In this study, we integrated metabolomic and transcriptomic profiling to systematically elucidate the biosynthetic and regulatory pathways during plumule development. A total of 59 flavonoid compounds were identified, with flavone glycosides being the predominant class. We observed a continuous increase in total flavonoid content from 12 to 24 days after pollination. Through transcriptome profiling, we reconstructed the flavonoid biosynthetic pathway and identified two candidate <ce:italic>C</ce:italic>-glycosyltransferase (<ce:italic>CGT</ce:italic>) genes. Functional validation via transient overexpression assays in lotus petals confirmed that <ce:italic>NnCGTa</ce:italic> promotes the accumulation of flavonoid C-glycosides, such as schaftoside and vitexin. Additionally, we characterized the highly expressed R2R3-MYB transcription factor <ce:italic>NnMYB12</ce:italic> as a potential key regulatory hub through <ce:italic>in vivo</ce:italic> assays. Its transient overexpression significantly increased flavonoid accumulation and upregulated the expression of flavonoid biosynthetic structural genes in lotus petals and tobacco leaves. In vivo experiments demonstrated that NnMYB12 directly bound to the promoter region of the <ce:italic>NnF3′H</ce:italic> gene. Our study provides <ce:italic>in planta</ce:italic> functional validation of genes governing flavonoid biosynthesis and regulation specifically within lotus plumule. These findings establish a theoretical framework for further exploration of flavonoid biosynthetic pathways in lotus and offer promising targets for breeding high-flavonoid lotus cultivars.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"12 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148287504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}