Horticultural Plant Journal最新文献

{"title":"Banana defense response against pathogens: Breeding disease-resistant cultivars","authors":"Hongyun Zeng, Yuanli Wu, Linbing Xu, Junhui Dong, Bingzhi Huang","doi":"10.1016/j.hpj.2024.08.004","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.08.004","url":null,"abstract":"Banana (<ce:italic>Musa</ce:italic> spp.), being a globally significant fruit crop, faces a myriad of threats from various diseases, such as <ce:italic>Fusarium</ce:italic> wilt, <ce:italic>Xanthomonas</ce:italic> wilt, bunchy top disease, and weevils disease. This review provides an overview of recent advancements in molecular mechanisms and immune signaling pathways underlying disease resistance in banana. First, the review discusses the latest research advances on banana pests and diseases. Subsequently, this review explores the immune responses and signaling pathways, pattern recognition receptor-triggered immunity, effector-triggered immunity, cell death, reactive oxygen species, autophagy, hormonal pathways, and other players involved in banana–disease interactions. Finally, the review discusses the current understanding of the genetic architecture of disease resistance in banana, focusing on the identification of defense-related genes and quantitative trait loci associated with resistance to major pathogens and offering recommendations for genetic research. The conclusion underscores the significance of research on banana immunity, specifically highlighting the crucial need to identify endogenous resistance genes and elucidate immune signaling pathways for future efforts aimed at breeding disease-resistant banana. This review offers a comprehensive perspective on the molecular mechanisms underlying disease resistance in banana and serves as a valuable reference for breeding efforts aimed at enhancing banana's resistance to pathogens.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"256 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142696386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics analysis of peach-like aroma formation in fruits of wild strawberry (Fragaria nilgerrensis)","authors":"Qiang Cao, Ni Zhu, Genqian Lan, Bo Yuan, Jian Wang, M. James C. Crabbe, Ticao Zhang, Qin Qiao","doi":"10.1016/j.hpj.2024.06.009","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.06.009","url":null,"abstract":"The unique fruity aroma of strawberries enhances their appeal to consumers. <ce:italic>Fragaria nilgerrensis</ce:italic>, a wild strawberry distributed in Southwest China and known for its distinctive peach-like aroma, represents a valuable resource for improving the fragrance of cultivated strawberries. However, the molecular mechanism underlying the peach-like aroma biosynthesis remains largely unexplored. In this study, we integrated metabolomic and transcriptomic data across four developmental stages of <ce:italic>F. nilgerrensis</ce:italic> fruits to construct a detailed profile of volatile organic compounds (VOCs) and the associated gene expression alterations during fruit maturation. Our findings reveal that γ-decalactone, δ-decalactone, and γ-undecalactone are the primary compounds responsible for the pronounced peach-like aroma, with their levels showing a significant correlation with the activity of the FngFAD2 enzyme. The silencing of the <ce:italic>FngFAD2</ce:italic> gene through tobacco rattle viral (TRV) induction resulted in notable reductions in both the peach-like aroma and lactone content in the fruit. In addition, integrating dual luciferase assays, yeast one-hybrid (Y1H) and subcellular localization experiments, we also identified three transcription factors (FngDOF1.2, FngWRKY3, and FngWRI1) that enhance <ce:italic>FngFAD2</ce:italic> expression. These findings elucidate the molecular regulatory network involved in the complex developmental process of peach-like flavor in strawberry fruits. Additionally, our research also provides a foundation for the utilization of the wild strawberry as well as improving the flavor and quality of cultivated strawberries.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"64 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142696402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An efficient irrigation method for facility-cultivated grape trees at various stages of development","authors":"Xiaolong Wang, Chang Liu, Xiaohao Ji, Xiangbin Shi, Zhiqiang Wang, Baoliang Wang, Fengzhi Liu, Haibo Wang","doi":"10.1016/j.hpj.2024.03.014","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.03.014","url":null,"abstract":"Achieving global fruit demand can be realized through the application of agricultural technologies. However, it is crucial to first overcome the enormous agricultural challenges posed by the implementation of efficient irrigation technologies. To address the issues related to inadequate water supply and inefficient traditional fruit tree irrigation methods, we propose a cost-effective and efficient approach—the “439” field precision irrigation scheme. This scheme predicts four relative soil water content minimum thresholds (RSWCT<ce:inf loc=\"post\">s</ce:inf>) and maximum (RSWCT<ce:inf loc=\"post\">e</ce:inf>) thresholds (relative to the percentage of field capacity [FC]) for starting and ending irrigation. By exploring the relationship between RSWCT<ce:inf loc=\"post\">s</ce:inf>, RSWCT<ce:inf loc=\"post\">e</ce:inf> and fruit quality, we assessed the scheme's effectiveness. A practical case study was conducted on grape (<ce:italic>Vitis vinifera</ce:italic> L., ‘87-1’) cultivated in a facility from 2019 to 2022 to evaluate the scheme's impact on irrigation management. The results indicate that maintaining 70%–80 % FC from germination stage (GS) to end bloom stage (EBS), 70%–80 % FC from EBS to veraison stage (VS), 55%–70 % FC from VS to maturation stage (MS), and 55%–65 % FC from MS to deciduous stage (DS) improve single grain weight (SGW). Similarly, to improve total soluble solid content, 60%–80 % FC is suggested from GS to EBS, 70%–80 % FC from EBS to VS, 60%–70 % FC from VS to MS, and 60%–70 % FC from MS to DS. To improve peel strength and fruit quality index (FQI), 70%–80 % FC is recommended from GS to EBS, 60%–70 % FC from EBS to VS, 55%–65 % FC from VS to MS, and 55%–70 % FC from MS to DS. This management tool helps farmers optimize irrigation efficiency and increase profits by growing high-quality fruit. In summary, the implementation of the “439” field precision irrigation system, coupled with fruit quality analysis, holds promise for enhancing water efficiency in precision agriculture.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"6 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142696405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A feedback loop comprising RhMYB114 and RhMYB16 regulates anthocyanin accumulation and tissue acidification in Rosa hybrida","authors":"Yeni Chen, Guoyuan Shao, Linmei Chen, Huanyu Zhang, Yongyi Cui, Ping Luo","doi":"10.1016/j.hpj.2024.07.004","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.07.004","url":null,"abstract":"Coloration in rose (<ce:italic>Rosa hybrida</ce:italic>) petals is primarily determined by anthocyanin accumulation in vacuoles, and vacuolar acidification plays a central role in controlling the accumulation of this pigment. Nevertheless, the regulatory interplay between anthocyanin accumulation and tissue acidification processes remains somewhat unclear. The present study characterized an activator <ce:italic>RhMYB114</ce:italic> and a repressor <ce:italic>RhMYB16</ce:italic>, which functioned synergistically in anthocyanin accumulation and tissue acidification in rose. Transforming tobacco and roses by overexpression, the introduction of <ce:italic>RhMYB114</ce:italic> resulted in an increase in anthocyanin levels and a noticeable decrease in pH in the petal cells of both rose and tobacco, whereas <ce:italic>RhMYB16</ce:italic> introduction led to inverse effects. To further clarify the underlying the regulatory mechanisms, the yeast two-hybrid (Y2H), bimolecular fluorescence complementation (BiFC) and dual-luciferase (LUC) were employed. The results showed that RhMYB16 competed with RhMYB114, bound to RhbHLH3 or RhbHLH33, and inhibited its ability to induce the expression of genes related to anthocyanin biosynthesis and acidification. Our findings revealed a feedback mechanism for the regulation of anthocyanin synthesis and tissue acidification involving <ce:italic>RhMYB114</ce:italic>, which stimulated the transcriptional expression of <ce:italic>RhMYB16</ce:italic>, whose encoded protein RhMYB16, in turn, negatively regulated the transcriptional expression of <ce:italic>RhMYB114</ce:italic>. Therefore, this study underscores the pivotal roles of the RhMYB114–RhMYB16 loop in regulating anthocyanin synthesis and tissue acidification, offering insights into metabolic manipulation to enhance the aesthetic appeal of roses.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"15 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142696408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing grape breeding through an in vitro embryo germination technique without cold stratification","authors":"Zhenhua Liu, Jing Song, Yan Wang, Ailing Yan, Xiaoyue Wang, Huiling Wang, Jiancheng Ren, Haiying Xu, Pingyin Guan, Lei Sun","doi":"10.1016/j.hpj.2024.04.012","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.04.012","url":null,"abstract":"In grape breeding programs, the extensive planting of seedlings is a crucial aspect. However, grape seeds display distinct dormancy traits, necessitating a prolonged cold stratification process for dormancy release. In order to enhance the efficiency of breeding programs, this study presents an innovative <ce:italic>in vitro</ce:italic> embryo germination technique that eliminates the requirement for cold stratification of seeds. The method involves the disruption of peripheral tissue in grape seed embryos using a straightforward mechanical technique, resulting in the efficient production of a substantial quantity of seed embryos，with a germination rate of up to 88 % for these isolated embryos. These embryos are subsequently cultured <ce:italic>in vitro</ce:italic> to facilitate germination into seedlings, thereby eliminating the need for cold stratification. Consequently, grape seedlings can be obtained within a significantly reduced timeframe of 30–38 d, expediting the overall grape breeding process. This novel approach not only accelerates grape hybridization but also streamlines the selection of new grape varieties, contributing to an efficient and time-sensitive breeding methodology.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"19 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142696403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Managing virus diseases in citrus: Leveraging high-throughput sequencing for versatile applications","authors":"Hongming Chen, Zhiyou Xuan, Lu Yang, Song Zhang, Mengji Cao","doi":"10.1016/j.hpj.2024.07.003","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.07.003","url":null,"abstract":"In this review, the advantages and advances in applying high-throughput sequencing (HTS) in the management of viral diseases in citrus, along with some challenges, are discussed to provide perspectives on future prospects. Since the initial implementation of HTS in citrus virology, a substantial number of citrus viruses have been identified, with a notable increase in the last 7 years. The acquisition of viral genomes and various HTS-based omics analyses serve as crucial pillars for advancing research in the etiology, epidemiology, pathology, evolution, ecology, and biotechnology of citrus viruses. HTS has notably contributed to disease diagnosis, such as the diagnoses of concave gum and impietratura, as well as to the surveillance of new virus risks and the preparation of virus-free materials. However, certain inherent defects in HTS and coupled bioinformatics analysis, such as challenges with sequence assembly and the detection of viral dark matter, require improvement to enhance practical efficiency. In addition, the utilization of HTS for the systematic management of citrus viral diseases remains limited, and drawing insights from other virus–plant pathosystems while integrating emerging compatible techniques and ideas may broaden its specific applications.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"70 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142670350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BcAHL24-MF1 promotes photomorphogenesis in Brassica campestris via inhibiting over-elongation of hypocotyl under light conditions","authors":"Huiyan Zhou, Jingwen Chen, Xiaojie Cai, Xiangtan Yao, Xinhua Quan, Songhua Bai, Jinzhuang Ni, Yujing Shao, Li Huang","doi":"10.1016/j.hpj.2024.05.015","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.05.015","url":null,"abstract":"Hypocotyl length is regarded to be a crucial seedling trait, influencing many subsequent plant development processes. However, little is known about this trait in <ce:italic>Brassica campestris</ce:italic> syn. <ce:italic>Brasscia rapa</ce:italic>. Here, we performed a comparative observation on the early hypocotyl development between two cultivars, ‘SZQ’ belonging to pak-choi (<ce:italic>B. campestris</ce:italic> ssp. <ce:italic>chinensis</ce:italic> var. <ce:italic>communis</ce:italic>) with longer hypocotyls, and ‘WTC’ belonging to Tacai (<ce:italic>B</ce:italic>. <ce:italic>campestris</ce:italic> L. ssp. <ce:italic>chinensis</ce:italic> var. <ce:italic>rosularis</ce:italic>) with shortter hypocotyls, and found that the difference in auxin biosynthesis might contribute to the varied hypocotyl phenotype between these two cultivars. By applying GWAS analysis using a total of 226 <ce:italic>B. campestris</ce:italic> accessions, we identified that the AT-Hook motif nuclear localized (AHL) gene <ce:italic>BcAHL24-MF1</ce:italic> contributed to the natural variation in hypocotyl length. Functional variation of BcAHL24-MF1 was attributed to four haplotypes featuring four SNPs within the promoter region, of which Hap I accumulated more transcripts with shorter hypocotyls. Constitutive overexpression of <ce:italic>BcAHL24-MF1</ce:italic> in <ce:italic>B. campestris</ce:italic> caused decreased hypocotyl length under light circumstances and even constant darkness, as BcAHL24-MF1 repressed the PIF-mediated transcriptional activation of auxin biosynthesis genes <ce:italic>BcYUC6-MF2</ce:italic> and <ce:italic>BcYUC8-LF.</ce:italic> Our research uncovered the important role of BcAHL24-MF1 in regulating light-triggered inhibition of hypocotyl elongation, therefore presenting a valuable genetic target for crop breeding.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"172 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142670351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BoaCRTISO regulates the color and glossiness of Chinese kale through its effects on pigment, abscisic acid, and cuticular wax biosynthesis","authors":"Chenlu Zhang, Ling Li, Yutong Zhang, Qiannan Liang, Sha Luo, Zhi Huang, Huanxiu Li, Victor Hugo Escalona, Zhifeng Chen, Fen Zhang, Yi Tang, Bo Sun","doi":"10.1016/j.hpj.2024.06.008","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.06.008","url":null,"abstract":"Carotenoid isomerase (CRTISO) is an important enzyme in carotenoid biosynthesis, and it catalyzes the conversion of lycopene precursors to lycopene in several plant species. However, the role of CRTISO in other biochemical processes during plant growth and development remains unclear. Here, we showed that Chinese kale <ce:italic>boacrtiso</ce:italic> mutants have distinctive characteristics, including a yellow-green hue and glossy appearance, and this contrasts with the dark green and glaucous traits observed in wild-type (WT) plants. Analysis of pigments in mutants revealed that the reduction in the content of carotenoids and chlorophylls contributed to the yellow-green coloration observed in mutants. An examination of cuticular waxes in Chinese kale indicated that there was a decrease in both the total wax content and the content of individual waxes in <ce:italic>boacrtiso</ce:italic> mutants (bearing a mutation of <ce:italic>BoaCRTISO</ce:italic>), which may be caused by the decrease of abscisic acid (ABA) content. The expression of carotenoid, chlorophyll, ABA, and wax biosynthesis genes was down-regulated in <ce:italic>boacrtiso</ce:italic> mutants. This finding confirms that <ce:italic>BoaCRTISO</ce:italic> regulates the biosynthesis of pigments, ABA, and cuticular waxes in Chinese kale. Our results provide new insights into the interplay between plant pigment and cuticular wax metabolic pathways in <ce:italic>Brassica</ce:italic> vegetables.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"13 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142670352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of the main terpenoids biosynthesis and accumulation in fruit trees","authors":"Yujie Hu, Tianyi Zheng, Jie Dong, Wangze Li, Xiaoyu Ma, Jin Li, Yulin Fang, Keqin Chen, Kekun Zhang","doi":"10.1016/j.hpj.2024.08.002","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.08.002","url":null,"abstract":"Terpenoids are vital secondary metabolites in plants that function as agents for defense and stress resistance. These genes not only play crucial roles in plant growth and development but also function in diverse biological group interactions. Terpenoids released by fruit trees possess defensive properties and constitute a class of aromatic compounds. For some fruits, terpenoids are indispensable indicators for evaluating fruit quality and the economic value. Significant research progress has been made in terpenoids biosynthesis and regulation. In this review, we introduce the main terpenoids of fruit trees, emphasize synthetic enzymes and regulatory factors involved in the mevalonate pathway and the methylerythritol pathway, and analyze <ce:italic>TPS</ce:italic> gene family identification and diversity in several fruit tree species. Moreover, the regulation of terpenes biosynthesis, including the molecular interaction mechanisms of environmental factors and hormone signaling pathways, are comprehensively described. Our objective is to summarize the molecular regulatory network and research foundation of terpenoids biosynthesis, providing a reference for investigations of metabolic pathways and promoting the development of techniques for the regulation and breeding of terpenoids in fruit trees.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"13 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142670353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Betula platyphylla glucosyltransferase BpGT14;6 is essential for cell wall development and stress response","authors":"Xiaohui Chen, Ruijia Zhang, Jialin Yan, Xinying Jia, Ronghua Liang, Fengkun Sun, Leilei Li, Minghao Ma, Yaguang Zhan, Fansuo Zeng","doi":"10.1016/j.hpj.2024.05.014","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.05.014","url":null,"abstract":"Glycosyltransferases (GTs) constitute a diverse family of synthetic polysaccharides with important roles in plant growth and development. This study characterized the GT14 family gene <ce:italic>BpGT14;6</ce:italic> of birch (<ce:italic>Betula platyphylla</ce:italic> Suk.). <ce:italic>BpGT14;6</ce:italic> was highly expressed in the xylem and stem of birch plants. Subcellular localization analysis suggested that <ce:italic>BpGT14;6</ce:italic> was located in the Golgi apparatus. RNA interference (RNAi) silencing of <ce:italic>BpGT14;6</ce:italic> revealed lower lignin, hemicellulose, and pectin contents compared to wild type (WT) plants. Following treatment with abscisic acid (ABA), compared to WT plants, RNAi-<ce:italic>BpGT14;6</ce:italic> plants were more sensitive to ABA, suffered more membrane lipid damage, and accumulated more reactive oxygen species. The inhibition of <ce:italic>BpGT14;6</ce:italic> expression narrowed the birch xylem and thinned the cell wall, and increased the expression of multiple ABA pathway-related genes in birch under ABA treatment. Compared to WT plants, RNAi-<ce:italic>BpGT14;6</ce:italic> plants showed increased tolerance to drought stress. Promoter analysis revealed that <ce:italic>BpGT14;6</ce:italic> is involved in hormone regulation and adaptation to adversity. Using the 1156 bp <ce:italic>BpGT14;6</ce:italic> promoter as bait, two potential transcription factors, BpWRKY1 and BpARF2, were identified through Y1H screening that may regulate its expression. EMSA confirmed that BpWRKY1 and BpARF2 can directly bind to the W-BOX and AuxRE <ce:italic>cis</ce:italic>-acting elements on the <ce:italic>BpGT14;6</ce:italic> promoter, respectively. The collective results suggest that <ce:italic>BpGT14;6</ce:italic> affects birch xylem and cell wall development by affecting lignin, hemicellulose, and pectin synthesis, and participates in birch adversity adaptation.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"49 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142670354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}