Horticultural Plant Journal最新文献

{"title":"PbrMYB4, a R2R3-MYB protein, regulates pear stone cell lignification through activation of lignin biosynthesis genes","authors":"Dongliang Liu, Yongsong Xue, Runze Wang, Bobo Song, Cheng Xue, Yanfei Shan, Zhaolong Xue, Jun Wu","doi":"10.1016/j.hpj.2023.09.004","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.09.004","url":null,"abstract":"<p>Pear (<em>Pyrus bretschneideri</em>) fruit stone cells are primarily composed of lignin and have strongly lignified cell walls. The presence of stone cells has a negative influence on fruit texture and taste, and thus the reduction of stone cell content in pear fruit is a key goal of breeding efforts. However, research into the key transcription factors and regulatory networks associated with pear fruit stone cell formation have been limited. We here used a combination of co-expression network and expression quantitative trait locus (eQTL) analyses in 206 pear cultivars with different stone cell contents to identify relevant genes; these analyses uncovered the gene <em>PbrMYB4,</em> a R2R3 MYB transcription factor gene. There was a strong positive correlation between relative <em>PbrMYB4</em> expression levels in the fruit flesh and stone cell/lignin contents. Overexpression of <em>PbrMYB4</em> significantly increased the lignin contents, whereas silencing of <em>PbrMYB4</em> had the opposite effect, decreasing the contents of lignin. <em>PbrMYB4</em> overexpression in pear calli significantly promoted lignin biosynthesis. In <em>Arabidopsis thaliana</em>, <em>PbrMYB4</em> overexpression resulted in increasing lignin deposition, cell wall thickness of vessels and xylary fiber, and accelerating expression level of lignin biosynthetic genes. PbrMYB4 was found to activate 4-Coumarate:Coenzyme A Ligase (<em>Pbr4CL1</em>) by binding to AC-I elements in the promoter regions, as demonstrated with dual-luciferase reporter assays and a yeast one-hybrid assay. These results demonstrated that <em>PbrMYB4</em> positively regulated lignin biosynthesis in pear fruit stone cells by activating lignin biosynthesis genes. This study improves our understanding of the gene regulatory networks associated with stone cell formation in pear fruit, providing guidance for molecular breeding of pear varieties with low stone cell content.</p>","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139565780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic variations at BBX24 and MYB110a loci regulated anthocyanin accumulation in pear bud sports","authors":"Yuhao Gao, Hongxu Li, Zhiwei Wang, Huabai Xue, Jianzhao Li, Wenjie Yu, Jiaxin Zhang, Junbei Ni, Yuanwen Teng, Songling Bai","doi":"10.1016/j.hpj.2024.01.001","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.01.001","url":null,"abstract":"<p>Genetic variation contributes to the phenotypic diversity of plants. Most red pear cultivars that accumulate anthocyanins originated from somatic variation and are termed bud sports. ‘Hongzaosu’ is a bud sport derived from the green pear ‘Zaosu’. A new genetic map was constructed from a population derived from the cross of ‘Yuluxiang’ and ‘Hongzaosu’, from which <em>PpBBX24</em> was identified as a crucial gene controlling anthocyanin accumulation. Genetic and phylogenetic evidence revealed that PpBBX24 is a repressor of anthocyanin biosynthesis in pear. A 14 bp deletion was detected in the third exon of <em>PpBBX24</em> in ‘Hongzaosu’, resulting in premature termination of protein translation. The truncated PpBBX24 protein was a positive regulator of anthocyanin biosynthesis by activating the transcription of <em>PpCHS</em> and <em>PpUFGT</em>. Three independent variations in the <em>BBX24</em> coding region that led to premature termination of translation were detected in other pear bud sports and the progeny of a bud sport that all accumulate anthocyanins. The genome of ‘Hongzaosu’ was assembled using both long-read and short-read sequences. By combining genomic and transcriptomic data, allele-specific expression of <em>PpMYB110a</em> was observed in the fruit skin of ‘Hongzaosu’, which was likely caused by large variation in the promoter. This work enhances understanding of coloration in red pears and provides a novel genomic resource for further studies on ‘Hongzaosu’ pear.</p>","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139506043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Mechanism of Flowering Time Regulation in Brassica rapa: Similarities and Differences with Arabidopsis","authors":"Na Li, Rui Yang, Shuxing Shen, Jianjun Zhao","doi":"10.1016/j.hpj.2023.05.020","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.05.020","url":null,"abstract":"<p>Properly regulated flowering time is pivotal for successful plant reproduction. The floral transition from vegetative growth to reproductive growth is regulated by a complex gene regulatory network that integrates environmental signals and internal conditions to ensure that flowering takes place under favorable conditions. <em>Brassica rapa</em> is a diploid Cruciferae species that includes several varieties that are cultivated as vegetable or oil crops. Flowering time is one of the most important agricultural traits of <em>B</em>. <em>rapa</em> crops because of its influence on yield and quality. The transition to flowering in <em>B</em>. <em>rapa</em> is regulated by several environmental and developmental cues, which are perceived by several signaling pathways, including the vernalization pathway, the autonomous pathway, the circadian clock, the thermosensory pathway, and gibberellin (GA) signaling. These signals are integrated to control the expression of floral integrators <em>BrFTs</em> and <em>BrSOC1s</em> to regulate flowering. In this review, we summarize current research advances on the molecular mechanisms that govern flowering time regulation in <em>B</em>. <em>rapa</em> and compare this to what is known in <em>Arabidopsis</em>.</p>","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139420069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Finding the Balance: Modifying the Cholesterol and Steroidal Glycoalkaloid Synthesis Pathway in Tomato (Solanum lycopersicum L.) for human health, fruit flavor, and plant defense","authors":"Vincenzo Averello IV, Adrian D. Hegeman, Changbin Chen","doi":"10.1016/j.hpj.2023.05.019","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.05.019","url":null,"abstract":"<p>Unlike most plants, members of the genus <em>Solanum</em> produce cholesterol and use this as a precursor for steroidal glycoalkaloids. The production of the compounds begins as a branch from brassinosteroid biosynthesis, which produces cholesterol that is further modified to produce steroidal glycoalkaloids. During the cholesterol biosynthesis pathway, genetic engineering could alter the formation of cholesterol from provitamin D₃ (7-dehydrocholesterol) and produce vitamin D₃. Cholesterol is a precursor for many steroidal glycoalkaloids, including <em>α</em>-tomatine and esculeoside A. Alpha-tomatine is consumed by mammals and it can reduce cholesterol content and improve LDL:HDL ratio. When there is a high <em>α</em>-tomatine content, the fruit will have a bitter flavor, which together with other steroidal glycoalkaloids serving as protective and defensive compounds for tomato against insect, fungal, and bacterial pests. These compounds also affect the rhizosphere bacteria by recruiting beneficial bacteria. One of the steroidal glycoalkaloids, esculeoside A increases while fruit ripening. This review focuses on recent studies that uncovered key reactions of the production of cholesterol and steroidal glycoalkaloids in tomato connecting to human health, fruit flavor, and plant defense and the potential application for tomato crop improvement.</p>","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139420005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification, characterization, and verification of miR399 target gene in grape","authors":"Maosong Pei,&nbsp;Hainan Liu,&nbsp;Tonglu Wei,&nbsp;Huiying Jin,&nbsp;Yihe Yu,&nbsp;Mengting Ma,&nbsp;Xiaomeng Song,&nbsp;Rundong Dai,&nbsp;Dalong Guo","doi":"10.1016/j.hpj.2023.02.009","DOIUrl":"10.1016/j.hpj.2023.02.009","url":null,"abstract":"<div><p>The microRNA miR399 plays an important role in phosphorus signal transduction pathways in plants. Previously, miR399 was shown to be closely associated with berry ripening in grape (<em>Vitis vinifera</em>). The objective of the present study was to elucidate the evolutionary characteristics of the miR399 gene family in grape and to verify the cleavage effect on the target genes. Grape miR399s were identified by miRNA sequencing and retrieval from the miRBase database. The mature sequences and precursor sequences were subjected to phylogenetic analysis to reconstruct evolutionary trees, as well as secondary structure analysis of the precursor sequence, and prediction of target genes. The <em>cis</em>-acting elements in the miR399 promoter were predicted and the cleavage effect of grape miR399b on its target genes was verified. The grape miR399 family comprised nine precursor sequences and nine mature sequences. The precursor sequences formed a typical and stable stem–loop structure. The minimum folding free energy ranged from −55.70 kcal · mol⁻¹ to −37.40 kcal · mol⁻¹. Multiple sequence alignment revealed that the miR399 family was highly conserved. The grape miR399 family was phylogenetically closely related to peach, apple, and citrus miR399s. Grape miR399s were predicted to target <em>inorganic phosphate transporter 1–3</em>, <em>phospholipase D delta-like</em>, and <em>beta-glucuronosyltransferase</em>. The cleavage effect of grape miR399b on the target genes was verified by means of a dual-luciferase assay and 5′ RLM-RACE. Histochemical GUS staining showed that the promoter activity of miR399b was promoted by GA₃ treatment.</p></div>","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468014123000298/pdfft?md5=efb0cd013b6318eba6dcdb7d624ab9b2&pid=1-s2.0-S2468014123000298-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74853619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of long-distance mobile mRNAs from shoot to root in grafted plant species","authors":"Mengmeng Fu ,&nbsp;Zhen Xu ,&nbsp;Huaying Ma ,&nbsp;Yifan Hao ,&nbsp;Ji Tian ,&nbsp;Yi Wang ,&nbsp;Xinzhong Zhang ,&nbsp;Xuefeng Xu ,&nbsp;Zhenhai Han ,&nbsp;Ting Wu","doi":"10.1016/j.hpj.2023.05.009","DOIUrl":"10.1016/j.hpj.2023.05.009","url":null,"abstract":"<div><p>Thousands of long-distance mobile mRNAs were identified from different grafting systems, based on high-throughput sequencing technology. Moreover, the long-distance delivery of RNAs was proved to involve multiple mechanisms. Here, we analyzed the homology, motif, and tRNA-like structure (TLS) of long-distance mobile mRNAs identified by RNA-seq as well as the RNA-binding protein (RBP) in nine grafting combinations including <em>Arabidopsis thalian</em>a, <em>Vitis vinifera</em>, <em>Cucumis sativus</em>, <em>Citrullus lanatus</em>, <em>Nicotiana benthamiana</em>, <em>Malus domestica</em>, <em>Pyrus</em> spp., <em>Glycine max</em> and <em>Phaseolus vulgaris</em>. Although several mRNAs were found to be shared in herbaceous, woody, and related species, the vast majority of long-distance mobile mRNAs were species-specific. Four non-specific movement-related motifs were identified, while the TLS was not necessary for mRNA long distance mobility. In addition, we found that RBPs were conserved among herbaceous and woody plants as well as related species. This paper reports a further in-depth analysis of the endogenous mechanisms by which the species-specific transportable mRNAs were selected by bioinformatics, in order to provide insights for future research on long-distance mobile mRNAs.</p></div>","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468014123000894/pdfft?md5=86d9e71f83fd608b5386777edec88def&pid=1-s2.0-S2468014123000894-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76584989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allotetraploidization event of Coptis chinensis shared by all Ranunculales","authors":"Yan Zhang ,&nbsp;Weina Ge ,&nbsp;Jia Teng ,&nbsp;Yanmei Yang ,&nbsp;Jianyu Wang ,&nbsp;Zijian Yu ,&nbsp;Jiaqi Wang ,&nbsp;Qimeng Xiao ,&nbsp;Junxin Zhao ,&nbsp;Shaoqi Shen ,&nbsp;Yishan Feng ,&nbsp;Shoutong Bao ,&nbsp;Yu Li ,&nbsp;Yuxian Li ,&nbsp;Tianyu Lei ,&nbsp;Yuxin Pan ,&nbsp;Lan Zhang ,&nbsp;Jinpeng Wang","doi":"10.1016/j.hpj.2023.01.004","DOIUrl":"10.1016/j.hpj.2023.01.004","url":null,"abstract":"<div><p><em>Coptis chinensis</em> Franch<em>.,</em> also named Chinese goldthread is a member of Ranunculaceae in the order Ranunculales and represents an important lineage of early eudicots with traditional medicinal value. In our study, by using syntenic analysis combined with phylogenomic analysis of <em>C. chinensis</em> and four other representative genomes from basal and core eudicots, we confirmed that the WGD event in <em>C. chinensis</em> was shared by <em>Aquilegia coerulea</em> and <em>Papaver somniferum</em> L. and quickly occurred after Ranunculales diverged from other eudicots, likely a Ranunculales common tetraploidization (RCT). The synonymous nucleotide substitutions at synonymous sites distribution of syntenic blocks across these genomes showed that the evolutionary rate of the <em>P. somniferum</em> genome is faster than that of the <em>C. chinensis</em> genome by approximately 13.7%, possibly due to Papaveraceaes having an additional special tetraploidization event (PST). After <em>Ks</em> correction, the RCT dated to 115–130 million years ago (MYA), which was close to the divergence of Ranunculaceaes and Papaveraceaes approximately 115.45–130.51 MYA. Moreover, we identified homologous genes related to polyploidization and speciation and constructed multiple sequence alignments with different reference genomes. Notably, the event-related subgenomes in the basal genomes all showed genomic fractionation bias, suggesting a likely allopolyploid nature of the RCT, PST and T-Alpha and T-Beta events in <em>Tetracentron sinense</em>. In addition, we detected that the sixteen P450 subfamilies were markedly expanded in the genomes of Ranunculales, and most of them were related to the RCT and PST events. We constructed a new platform for Early Eudicot Comparative Genomic Research (<span>http://www.cgrpoee.top/index.html</span><svg><path></path></svg>) to store more information. In summary, our findings support the WGD of <em>C. chinensis</em> shared by Ranunculales, which is likely an allotetraploidization event. This present effort offered new insights into the evolution of key polyploidization events and the genes related to secondary metabolites during the diversification of early eudicots.</p></div>","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468014123000043/pdfft?md5=297629accfe2ad41fda7088c623b4905&pid=1-s2.0-S2468014123000043-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79745897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The chromosome-level genome of double-petal phenotype jasmine provides insights into the biosynthesis of floral scent","authors":"Xiangyu Qi ,&nbsp;Huadi Wang ,&nbsp;Shuyun Liu ,&nbsp;Shuangshuang Chen ,&nbsp;Jing Feng ,&nbsp;Huijie Chen ,&nbsp;Ziyi Qin ,&nbsp;Quanming Chen ,&nbsp;Ikram Blilou ,&nbsp;Yanming Deng","doi":"10.1016/j.hpj.2023.03.006","DOIUrl":"10.1016/j.hpj.2023.03.006","url":null,"abstract":"<div><p>Jasmine (<em>Jasminum sambac</em> Aiton) is a well-known cultivated plant species for its fragrant flowers used in the perfume industry and cosmetics. However, the genetic basis of its floral scent is largely unknown. In this study, using PacBio, Illumina, 10× Genomics and high-throughput chromosome conformation capture (Hi-C) sequencing technologies, a high-quality chromosome-level reference genome for <em>J. sambac</em> was obtained, exploiting a double-petal phenotype cultivar ‘Shuangbanmoli’ (JSSB). The results showed that the final assembled genome of JSSB is 580.33 Mb in size (contig N50 = 1.05 Mb; scaffold N50 = 45.07 Mb) with a total of 39 618 predicted protein-coding genes. Our analyses revealed that the JSSB genome has undergone an ancient whole-genome duplication (WGD) event at 91.68 million years ago (Mya). It was estimated that <em>J. sambac</em> diverged from the lineage leading to <em>Olea europaea</em> and <em>Osmanthus fragrans</em> about 28.8 Mya. On the basis of a combination of genomic, transcriptomic and metabolomic analyses, a range of floral scent volatiles and genes were identified involved in the benzenoid/phenylpropanoid and terpenoid biosynthesis pathways. The results provide new insights into the molecular mechanism of its fragrance biosynthesis in jasmine.</p></div>","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468014123000456/pdfft?md5=c3a7b70b5b3feceb2d427df7dceb7933&pid=1-s2.0-S2468014123000456-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80894793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"R2R3-type LoMYB21 affects jasmonate-regulated development and dehiscence of anthers in lily (Lilium oriental hybrids)","authors":"Zheng Tong ,&nbsp;Tingting Dong ,&nbsp;Qiuhua Li ,&nbsp;Rui Wang ,&nbsp;Junna He ,&nbsp;Bo Hong","doi":"10.1016/j.hpj.2023.04.004","DOIUrl":"10.1016/j.hpj.2023.04.004","url":null,"abstract":"<div><p>Lilies are widely cultivated for cut flowers, but their large anthers carry a considerable amount of colored pollen that is dispersed easily. Studying the molecular mechanism of anther development and dehiscence could help solve this problem. <em>LoMYB21</em>, encoding a putative R2R3 v-myb avian myeloblastosis viral oncogene homolog (MYB) transcription factor, was identified from oriental lilies (<em>Lilium</em> ‘Siberia’). Real-time quantitative PCR analysis showed that <em>LoMYB21</em> was mainly expressed in the anther, filament and stigma and had high expression during the late stages of lily anther development. LoMYB21 had transactivation activity and was located in the nucleus through yeast one-hybrid assays and transient expression in <em>Nicotiana benthamiana.</em> Suppression of <em>LoMYB2</em>1 by virus-induced gene silencing (VIGS) in <em>Lilium</em> ‘Siberia’ led to anther indehiscence and reduced the expression of genes related to Jasmonate acid (JA) biosynthesis and signal transduction. Induction of <em>LoMYB21</em> in DEX::<em>LoMYB21</em> transgenic <em>Arabidopsis</em> caused procumbent inflorescences that became infertile, accompanied by higher expression of JA biosynthetic and signaling genes. These results demonstrated that JA content and signaling were abnormal in silenced lily and transgenic <em>LoMYB21 Arabidopsis</em>, which affected anther development. Our study indicated that <em>LoMYB21</em> could regulate lily anther dehiscence through JA biosynthesis and signaling during the late stages of anther development.</p></div>","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468014123000651/pdfft?md5=6b39d0a8cd5e8ef03e41f3ea39bcf8c7&pid=1-s2.0-S2468014123000651-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87522946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CsABF3-activated CsSUT1 pathway is implicated in pre-harvest water deficit inducing sucrose accumulation in citrus fruit","authors":"Xiaochuan Ma ,&nbsp;Yuanyuan Chang ,&nbsp;Feifei Li ,&nbsp;Junfeng Yang ,&nbsp;Li Ye ,&nbsp;Tie Zhou ,&nbsp;Yan Jin ,&nbsp;Ling Sheng ,&nbsp;Xiaopeng Lu","doi":"10.1016/j.hpj.2023.07.001","DOIUrl":"10.1016/j.hpj.2023.07.001","url":null,"abstract":"<div><p>Pre-harvest water deficit (PHWD) plays an important role in sugar accumulation of citrus fruit. However, the mechanism is not known well. Here, it was confirmed that PHWD promoted sucrose accumulation of citrus fruit, but had limited effect on fructose, glucose and total acid. A sucrose transporter, CsSUT1, which localizes to the plasma membrane, was demonstrated to function in sucrose transport induced by PHWD. Compared to wild-type, <em>CsSUT1</em> overexpression in citrus calli stimulated sucrose, fructose and glucose accumulation, while its silencing in juice sacs reduced sucrose accumulation. Increased sugar accumulation in transgenic lines enhanced plant drought tolerance, and resulted in decreased electrolyte leakage, malondialdehyde and hydrogen peroxide contents, as well as increased superoxide dismutase activity and proline contents. An abscisic acid (ABA)-responsive transcription factor, <em>CsABF3</em>, was found with a same expression pattern with <em>CsSUT1</em> under PHWD. Yeast one-hybrid, electrophoretic mobility shift assay and dual-luciferase assays all revealed that CsABF3 directly bound with the <em>CsSUT1</em> promoter by ABA responsive elements. When <em>CsABF3</em> was overexpressed in citrus calli, the sucrose, fructose and glucose concentration increased correspondingly. Further, transgenic studies demonstrated that CsABF3 could affect sucrose accumulation by regulating <em>CsSUT1</em>. Overall, this study revealed a regulation of CsABF3 promoting <em>CsSUT1</em> expression and sucrose accumulation in response to PHWD. Our results provide a detail insight into the quality formation of citrus fruit.</p></div>","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468014123001474/pdfft?md5=992bd371231a5c2ad4ca045b2e7f281d&pid=1-s2.0-S2468014123001474-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135484305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}