Horticultural Plant Journal最新文献

{"title":"Improving detection of potato virus X resistance genes Rx1 and Rx2 in tetraploid potato using optimized PCR and KASP markers","authors":"Saiful Islam, Jiana Li, Jie Zheng, Yingtao Zuo, Ying Zou, Tao Yu, Mohammad Ataur Rahman, Xianzhou Nie, Zhiguo Fang, Tao Luo, Botao Song, Bihua Nie","doi":"10.1016/j.hpj.2025.01.014","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.014","url":null,"abstract":"Potato virus X (PVX) seriously threatens global potato cultivation, necessitating a deeper understanding of the genetic mechanisms responsible for resistance. The <ce:italic>Rx1</ce:italic> and <ce:italic>Rx2</ce:italic> genes play a pivotal role in breeding potatoes with broad-spectrum PVX resistance. However, current DNA markers for these genes present uncertainties, as they are found in both resistant and susceptible materials, complicating the identification of the responsible resistance gene. This study aimed to develop robust high-throughput molecular markers, specifically Kompetitive Allele-Specific PCR (KASP) markers, to reliably detect the <ce:italic>Rx1</ce:italic> and <ce:italic>Rx2</ce:italic> genes. Genetic polymorphism analysis of seven resistant and four susceptible potato genotypes revealed seven single nucleotide polymorphisms (SNPs) and one insertion-deletion (Indel), along with corresponding amino acid changes in the Rx1 and Rx2 protein sequences. Based on these variations, high-throughput molecular markers were developed. Notably, the primer pairs Rx1-1R-F + Rx1-5R-R and Rx2-1R2-F + Rx2-2R2-R showed high accuracy, detecting <ce:italic>Rx1</ce:italic> and <ce:italic>Rx2</ce:italic> genes without false positives or false negatives. Optimized multiplex PCR protocols also demonstrated strong potential in simultaneously detecting both genes. KASP markers targeting SNPs at positions 2 515 (G to C) in the <ce:italic>Rx1</ce:italic> CDS and 2 445 (C to G) in the <ce:italic>Rx2</ce:italic> CDS efficiently differentiated between heterozygous resistant and homozygous susceptible genotypes. Furthermore, these markers effectively identified <ce:italic>Rx1</ce:italic> and <ce:italic>Rx2</ce:italic> genotypes in 243 and 133 progenies across multiple crosses. Progenies that tested positive for <ce:italic>Rx1</ce:italic> and <ce:italic>Rx2</ce:italic> through PCR or KASP assays exhibited extreme resistance (ER) to PVX, while negative progeny was susceptible. These findings highlight the potential of the developed markers to accelerate the breeding cycle and improve the production of PVX-resistant potato varieties.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"26 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin modified root architecture system and accelerated mineral nutrient concentration of different pepper species under vanadium toxicity","authors":"Muhammad Ahsan Altaf, Huangying Shu, Naveed Mushtaq, Huizhen Fu, Xu Lu, Shanhan Cheng, Zhiwei Wang","doi":"10.1016/j.hpj.2024.12.006","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.12.006","url":null,"abstract":"Heavy metal contamination has adversely affected crop productivity around the world. Vanadium (V) toxicity is a significant environmental factor that lowers agricultural productivity by inhibiting plant development, diminishing nutrient uptake, and damaging root morphology. Melatonin (ME) is a well-known powerful antioxidant and multifunctional molecule that improves the resilience of plants against environmental stressors. In this work, we investigated the response of different pepper species (<ce:italic>Capsicum annuum</ce:italic> L. (CA), <ce:italic>C. baccatum</ce:italic> L. (CB), <ce:italic>C. pubescens</ce:italic> Ruiz &amp; Pav. (CP) to V toxicity as well as the possible roles of ME in enhancing V stress tolerance. Pepper roots were pretreated with ME (5 to μmol. L<ce:sup loc=\"post\">−1</ce:sup>) for three days, then V (30 mg. L<ce:sup loc=\"post\">−1</ce:sup>) was applied for 2 weeks as a stress treatment. The findings showed that plants pretreated with ME exposed to V stress had improved root activity and biomass production. The root morphology and mineral nutrient accumulation of the pepper species were greatly improved by ME application. In addition, ME treatments lowered leaf V concentrations by lowering V translocation from root to shoot. In a nutshell, ME treatment modified root architecture system, increased concentration of mineral nutrient, and reduced V accumulation of pepper species under V stress.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"240 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UV-B and mechanical wounding synergistically induce α-farnesene biosynthesis via CsHY5-CsMYC2 cooperation during oolong tea processing","authors":"Jingjie Cao, Dandan Li, Xiaohui Wang, Xin He, Ying Sun, Xin Cheng, Xiaochun Wan, Linlin Liu","doi":"10.1016/j.hpj.2025.01.015","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.015","url":null,"abstract":"UV-B application enhances the aroma quality of oolong tea; however, the underlying regulatory mechanism remains unclear. This study investigates the regulatory role of UV-B in the biosynthesis of <ce:italic>α</ce:italic>-farnesene, an important floral and fruity characteristic aroma. UV-B treatment significantly improved the aroma quality of ‘Foshou’ and ‘Yuquan’ oolong teas, increasing <ce:italic>α</ce:italic>-farnesene levels by 1.8- and 1.4-fold, respectively. The <ce:italic>α-farnesene synthase</ce:italic> (<ce:italic>CsAFS</ce:italic>), <ce:italic>ELONGATED HYPOCOTYL 5</ce:italic> (<ce:italic>CsHY5</ce:italic>), and <ce:italic>myelocytomatosis protein 2</ce:italic> (<ce:italic>CsMYC2</ce:italic>) exhibited a highly correlated expression pattern closely associated with <ce:italic>α</ce:italic>-farnesene accumulation. Single-factor treatment revealed that <ce:italic>CsAFS</ce:italic> expression was induced by both UV-B and mechanical wounding, with <ce:italic>CsHY5</ce:italic> predominantly responding to UV-B radiation, while <ce:italic>CsMYC2</ce:italic> primarily responded to tumbling-induced mechanical wounding signal. Transient suppression of CsHY5 in tea leaves reduced the expression of both <ce:italic>CsAFS</ce:italic> and <ce:italic>CsMYC2</ce:italic> whereas <ce:italic>CsMYC2</ce:italic> suppression decreased <ce:italic>CsAFS</ce:italic> expression. G-box motifs were identified in promoters of <ce:italic>CsMYC2</ce:italic> and <ce:italic>CsAFS</ce:italic>, and the dual-luciferase reporter assay (LUC) and electrophoretic mobility shift assays (EMSA) demonstrated direct binding functions of CsHY5 to <ce:italic>CsAFS</ce:italic> and <ce:italic>CsMYC2</ce:italic> promoters, as well as CsMYC2 to the <ce:italic>CsAFS</ce:italic> promoter<ce:italic>.</ce:italic> Based on sensory evaluation, odourant quantification, gene expression, and molecular functional analysis, we propose that UV-B radiation and tumbling-induced wounding signals synergistically regulate <ce:italic>α</ce:italic>-farnesene biosynthesis through a coordinated interaction of CsHY5 and CsMYC2 during oolong tea processing. These findings improve our understanding of flavour formation during oolong tea production and also provide novel insights into artificial light application in tea manufacturing.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"42 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of RNA m6A on the formation of multi-petalization in Magnolia wufengensis: Mechanistic insights and gene expression analysis","authors":"Cunjie Li, Huarong Li, Jiang Ma, Xiaoning Fan, Yanjun Cai, Liyuan Chen, Hanlin Zhou, Hongwei Liang, Chao Zhou, Faju Chen","doi":"10.1016/j.hpj.2025.01.011","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.011","url":null,"abstract":"<ce:italic>N</ce:italic><ce:sup loc=\"post\">6</ce:sup>-Methyladenosine (m<ce:sup loc=\"post\">6</ce:sup>A) is the most common modification in the transcriptome of biological RNA and plays roles that include maintaining the stability and transportation of mRNA, mRNA precursor shearing, polyadenylation, and the initiation of translation. With the improving understanding of RNA methylation, m<ce:sup loc=\"post\">6</ce:sup>A modification is known to play vital roles in plant development and growth. The multi-petalization of flowering plants has high ornamental and research value in horticultural landscapes. However, the mechanism of RNA methylation in flower formation in <ce:italic>Magnolia wufengensis</ce:italic>, a classical multi-petalizational plant, remains unclear. This study compared and analyzed RNA m<ce:sup loc=\"post\">6</ce:sup>A methylation and the transcriptome in floral buds of two varieties with large differences in tepal number at the early stage of development. It was found that the degree of RNA m<ce:sup loc=\"post\">6</ce:sup>A methylation and relative expression levels of <ce:italic>MawuAGL6-2</ce:italic>, <ce:italic>MawuPI-4</ce:italic>, and <ce:italic>MawuAGL9</ce:italic> in ‘Jiaodan’ with 36 tepals were significantly higher than those in ‘Jiaohong’ with 9 tepals during the development of floral organ primordia. Combined with quantitative real-time PCR, the expression levels of <ce:italic>MawuAGL6-2</ce:italic>, <ce:italic>MawuPI-4</ce:italic>, and <ce:italic>MawuAGL9</ce:italic> were positively correlated with the number of tepals. Transgenic experiments showed that <ce:italic>MawuAGL6-1/2</ce:italic>, and <ce:italic>MawuPI-4</ce:italic> can increase the number of petals in <ce:italic>Arabidopsis</ce:italic>. Moreover, <ce:italic>MawuAGL6-2</ce:italic> and <ce:italic>MawuPI-4</ce:italic> can restore the missing petal phenotype of mutant <ce:italic>Arabidopsis</ce:italic>. Yeast two hybrid and yeast three hybrid indicated that MawuAGL6-2, MawuAP3-1/2, and MawuPI-4 could interact with each other under the mediation of the class E protein MawuAGL9. Based on these results, it is hypothesized that m<ce:sup loc=\"post\">6</ce:sup>A methylation influences the multi-petalization of <ce:italic>Magnolia wufengensis</ce:italic> by affecting the expression levels of <ce:italic>MawuAGL6-2</ce:italic>, <ce:italic>MawuAP3-1/2</ce:italic>, <ce:italic>MawuPI-4</ce:italic>, and <ce:italic>MawuAGL9</ce:italic>. These findings provide a better understanding of the molecular mechanisms of epigenetic modifications in flower developmental diversity.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"90 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative metabolome and transcriptome revealed the CpODO1 transcription factor controlling the varied floral scent traits of three wintersweet cultivars","authors":"Yubing Yong, Qi Zhou, Xin Ding, Jianming Cheng, Chengcheng Zhou, Zhiguo Shen, Lin Zhang","doi":"10.1016/j.hpj.2025.01.010","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.010","url":null,"abstract":"Wintersweet (<ce:italic>Chimonanthus praecox</ce:italic> L.), a well-known fragrant flowering shrub, is extensively planted for ornamental purpose and production of floral essential oil. Although the tepal color of wintersweet varieties exhibits the most remarkable diversity, variations in the floral scent traits are also noticeable across different cultivars. In this study, the floral volatile organic compounds (VOCs) in three wintersweet cultivars, ‘Yanlingsuxin’, ‘Yuxiang’, and ‘Hongyun’ were detected via GC–MS coupled with OAV. The distinct floral aromas of the three cultivars were primarily attributed to benzyl alcohol (abundant in ‘Yuxiang’), linalool (abundant in ‘Yanlingsuxin’), (−)-γ-cadinene and eugenol (abundant in ‘Hongyun’). Integrated analyses of metabolome and transcriptome showed that an R2R3-MYB transcription factor, <ce:italic>CpODO1</ce:italic>, potentially have a crucial regulatory function in controlling the production of multiple aroma compounds. Overexpression of <ce:italic>CpODO1</ce:italic> can enhance the production of benzyl alcohol in transgenic tobacco flowers. Analysis of DAP-seq data, EMSA and dual-luciferase assay revealed that CpODO1 predominantly regulate the expression of <ce:italic>CpCYP71</ce:italic>, a cytochrome P450 gene encoding a key enzyme in the production of benzyl alcohol, and the transcriptional regulation of <ce:italic>CpODO1</ce:italic> is driven by CpEOBII. The identification of polymorphisms in the MYB binding <ce:italic>cis</ce:italic>-motifs of <ce:italic>CpCYP71</ce:italic> and <ce:italic>CpODO1</ce:italic> promoters revealed the regulatory mechanism underlying the varied synthesis of benzyl alcohol in three wintersweet cultivars. This study provides new anchor points for floral scent quality improvement breeding of wintersweet, and the profusion of wintersweet germplasm can serve as a material basis for developing various aroma products.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"49 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete mitochondrial genome map and transcriptome reveal the mechanisms of a novel neau type CMS in Brassica rapa","authors":"Yan Liu, Xingliang Wang, Jiaqi Liu, Xianfeng Zhang, Wei Luan, Jiaxi Li, Gu ran, Yaowei Zhang","doi":"10.1016/j.hpj.2025.01.012","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.012","url":null,"abstract":"Cytoplasmic male sterility (CMS) is a widespread phenomenon in crops and is beneficial for commercial hybrid breeding. Although CMS systems in <ce:italic>Brassica</ce:italic> species are extensively utilized, they are derived from relatively limited sources, and the underlying molecular mechanisms are not well understood. In this study, a novel CMS line, named Neau, was identified through distant hybridization between <ce:italic>Matthiola incana</ce:italic> and <ce:italic>Brassica rapa</ce:italic>. Neau CMS exhibits agronomic traits similar to its maintainer line of Chinese cabbage and displays sterility at the tetrad stage. The mitochondrial genomes of Neau CMS and its maintainer line were assembled to 359 596 bp and 219 771 bp, respectively, encoding 99 and 66 unknown open reading frames (orfs). Six orfs were identified as specific to Neau CMS with sequence alignments, with <ce:italic>orf154</ce:italic> and two copies of <ce:italic>orf138</ce:italic> identified as candidate genes based on their transmembrane structures and their ability to encode toxic proteins that inhibited <ce:italic>Escherichia coli</ce:italic> cell growth. The expression of <ce:italic>orf154</ce:italic> was induced during the tetrad stage by quantitative real-time polymerase chain reaction(qRT-PCR), whereas <ce:italic>orf138a</ce:italic> showed higher expression at the microspore mononuclear stage in Neau CMS. Transcriptome analysis revealed reduced expressions of most pollen development-related genes, as well as genes involved in peroxidase/oxidative stress responses and the electron transfer chain in Neau CMS. Additionally, Neau CMS exhibited increased levels of O<ce:inf loc=\"post\">2</ce:inf><ce:sup loc=\"post\">.-</ce:sup>, H<ce:inf loc=\"post\">2</ce:inf>O<ce:inf loc=\"post\">2</ce:inf>, and malondialdehyde (MDA), alongside reduced activities of SOD, CAT, and POD. Both ATP content and ATPase activity were down-regulated in Neau CMS. Overexpression of <ce:italic>orf138</ce:italic> and <ce:italic>orf154</ce:italic> in <ce:italic>Arabidopsis thaliana</ce:italic> resulted in reductions in pollen quantity, viability, and seed production. These findings suggest that <ce:italic>orf138</ce:italic> and <ce:italic>orf154</ce:italic> are key candidate genes responsible for the sterility observed in Neau CMS in Chinese cabbage.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"37 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of growth promoting microorganisms on tomato growth parameters in interaction with root nematodes","authors":"Mohsen Rafiee, Majid Olia, Mehdi Nasr-Esfahani, Parisa Mashayekhi, Arman Nasr Esfahani","doi":"10.1016/j.hpj.2025.01.013","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.013","url":null,"abstract":"As we all are aware of the deleterious and ill effects of various chemical pesticides and inorganic fertilizers, the agricultural community has to move towards an alternative having a sustainable and eco-friendly approach. Thus in this study, we analyzed the efficiency of growth promoting microorganism products (GPMPs) using Tricuran-P (<ce:italic>Trichoderma harzianum</ce:italic> T-22); Nitroxin (<ce:italic>Azospirilium</ce:italic> and <ce:italic>Azotobacter</ce:italic> rhizobacteria in combination); Effective Micro-Organisms (EM) (photosynthetic bacteria, lactobacillus, Streptomyces, Actinomycetes, yeast, etc.); Phytohumic; Formycine Gold PX 20%; and Fenamiphos (Nemacur) on the tomato growth and physico-chemical properties of the plant and the soil at a greenhouse condition in interaction with root-knot nematodes (RKNs), <ce:italic>Meloidogyne javanica</ce:italic> pathosystem along with irrigation water at the interval of every three weeks for three times. The results showed that the lowest second-stage juveniles (J2) reproduction factor rate in the soil was related to Nemacur 18% and Tricuran-P 19%, followed by Formycine with 30% reduction in comparison to inoculated controls, respectively. Increasing the concentration of Formycine led to a significant decrease in the reproduction factor rate of J2 in the soil and the roots. Furthermore, in the biomass traits, the treatments showed a significant effect on the related traits, which were increased with the Formycine increase in the growth parameters of the biomass traits. The results of the effect of different treatments on physico-chemical traits of the soil indicated that the treatments had a significant difference on all the relevant traits except the concentration of phosphorus (P<ce:inf loc=\"post\">2</ce:inf>O<ce:inf loc=\"post\">5</ce:inf>) and potassium (K<ce:inf loc=\"post\">2</ce:inf>O). Whereas, the content of elements in the tomato plants showed a significant difference on all the evaluated traits. At the same time, the correlation between the attributes in the tomato plant with the soil indicated that the plant’s N had a positive and significant correlation with Organic Carbon (OC), total N, P<ce:inf loc=\"post\">2</ce:inf>O<ce:inf loc=\"post\">5</ce:inf>, K<ce:inf loc=\"post\">2</ce:inf>O and the soil moisture; and a negative and significant correlation with the soil’s EC (electrical conductivity). Moreover, the content of K, Ca, and Mg of the plant had a positive and significant correlation with the soil moisture, and a negative and significant correlation with the soil’s EC. Cumulatively, GPMPs not only are enable to shift over the soil environments in the favor of the plant growth, but also similarly modulate immunity against RKNs, by virtue of which Formycine and others can compete relatively compared to Fenamiphos (Nemacur) and other relevant GPMPs in this research.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"16 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The development of liquid-phase chip by target sequencing and their application in watermelon molecular breeding","authors":"Shixiang Duan, Dengke Wang, Qishuai Kang, Huazheng Yan, Jiaxin Cui, Minjuan Zhang, Dongming Liu, Sen Yang, Yingchun Zhu, Huanhuan Niu, Wenkai Yan, Huayu Zhu, Jianbin Hu, Junling Dou, Luming Yang","doi":"10.1016/j.hpj.2025.01.009","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.009","url":null,"abstract":"Genotyping by Target Sequencing (GBTS) technology, known for its flexibility, high efficiency, high throughput, and low cost, has been increasingly employed in molecular breeding. However, there is still limited study on the design and development of high-throughput genotyping tools in watermelon. In this study, we identified 112 000 high quality SNPs by analyzing the resequencing data of 43 cultivated watermelon accessions. 11 921 and 6094 SNPs were selected for developing two sets of watermelon liquid-phase chips with different marker densities, named Watermelon 10K and 5K, respectively. Furthermore, the SNPs and Indels of most mapped gene/QTLs for many agronomic important traits in watermelon were also integrated into the two chips for foreground selection. These chips have been tested using GBTS technology in various applications in watermelon. The genotyping of 76 accessions by Watermelon 5K liquid-phase chip showed an average detection rate of 99.28 % and 81.78 % for cultivated and wild watermelon accessions, respectively. This provided enough markers information for GWAS and two significant QTLs, <ce:italic>ssc1.1</ce:italic> and <ce:italic>ssc1.2</ce:italic>, associated with soluble sugar content were detected. Furthermore, BSA-seq analysis for non-lobed leaf and dwarf traits were validated by liquid-phase chips, and the candidate region was consistent with our previous studies. Additionally, we precisely introduced the <ce:italic>Cldw1</ce:italic> and <ce:italic>Clbl</ce:italic> genes into an elite inbred line WT2 using Watermelon 5K for assisted selection, resulting in the development of three new germplasm with good plant architecture. As a high-throughput genotyping liquid-phase SNP array, the Watermelon 10K and 5K chips will greatly facilitate functional studies and molecular breeding in watermelon.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"16 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined volatile metabolome and transcriptome analysis of 60 peach cultivars provide new insights into the formation of aroma and the identification of associated genes","authors":"Wenyi Duan, Zhenyu Yao, Chengkui Qiao, Junren Meng, Shihang Sun, Lei Pan, Liang Niu, Guochao Cui, Zhiqiang Wang, Wenfang Zeng","doi":"10.1016/j.hpj.2024.12.005","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.12.005","url":null,"abstract":"Volatile organic compounds (VOCs) are the main chemical compounds that determine the characteristic aroma and flavor of fruit. In this study, we identified a total of 97 VOCs, including 5C6 compounds, 12 aldehydes, 4 alcohols, 10 esters, 13 lactones, 18 terpenes, 8 norisoprenoids, 8 ketones, 3 hydrocarbons, 8 phenylalanine derivates, and 8 other compounds, in 60 peach cultivars using headspace solid-phase micro-extraction (HS-SPME) combined with gas chromatography-mass spectrophotometry (GC–MS). A wide range of VOCs were detected in these germplasm resources with respect to both quantity and concentration. Correlation analysis with different physiological traits demonstrated that the genetic background exerts a significant influence on the composition and content of VOCs among peach cultivars. For example, the content of norisoprenoid was significantly lower in yellow-fleshed peach than in white-fleshed peach, and lactones were almost undetectable in stony hard peach. Among the 97 VOCs, 26 exhibited odor activity values (OAVs) exceeding 1, suggesting that these compounds act as key odorants in the peach VOC composition. Moreover, six structural genes associated with the synthesis of γ-decalactone and (<ce:italic>Z</ce:italic>)-3-hexenyl acetate and five genes linked to aldehyde and 1-octen-3-one biosynthesis were identified through weighted gene co-expression network analysis (WGCNA). Additionally, 15 transcription factors (TFs) were identified as potentially regulating VOC synthesis. Overall, these data provide insight into the factors contributing to the differences in aroma qualities among peach cultivars, which can help to promote the development of peach breeding.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"26 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SBP transcription factor MdSPL13B positively regulates salt tolerance in apple","authors":"Xiuli Jing, Daru Wang, Xin Liu, Guolin Chen, Xinsong Guo, Xiaofei Wang, Zhenlu Zhang, Chunxiang You","doi":"10.1016/j.hpj.2025.01.008","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.008","url":null,"abstract":"Salt stress has a major effect on the quality and yield of crops, and many transcription factors (TFs), such as WRKY, NAC, and ERF TFs have been shown to participate in the regulation of salt stress. Squamosa promoter binding protein-like (SPL) TFs play a role in plant floral organ development, metal ion responses and disease resistance. However, the precise function of SPL TFs in regulating the salt stress in plants remains unclear. In this study, we investigated the mechanism by which SPL TFs regulate salt stress in apple (<ce:italic>Malus domestica</ce:italic>). Overexpression of <ce:italic>MdSPL13B</ce:italic> in apple calli revealed that it positively regulated salt stress. The heterologous transformation of <ce:italic>MdSPL3B</ce:italic> also resulted in significant salt tolerance in <ce:italic>Arabidopsis</ce:italic> and tomato. Subsequently, a yeast two-hybrid assay showed that MdSPL13B could interact with MdNAC29 and MdPYL4, which was further confirmed by the bimolecular fluorescence complementation assay. Additionally, overexpression of <ce:italic>MdNAC29</ce:italic> and <ce:italic>MdPYL4</ce:italic> in apple calli, <ce:italic>Arabidopsis</ce:italic> and <ce:italic>Nicotiana benthamiana</ce:italic> plants indicated that <ce:italic>MdNAC29</ce:italic> negatively regulated salt tolerance and that <ce:italic>MdPYL4</ce:italic> positively regulated salt tolerance. Furthermore, the yeast one-hybrid, electrophoretic mobility shift assay and dual-luciferase assay showed that MdSPL13B could enhance salt tolerance through activation of <ce:italic>MdWRKY100</ce:italic> in apple. In conclusion, our findings enhance the understanding of how <ce:italic>MdSPL3B</ce:italic> and its target gene regulate salt tolerance in apple, and have implications for the breeding of salt-tolerant apple.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"23 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}