Horticultural Plant Journal最新文献

{"title":"The role of m6A in plant development, stress response, and agricultural practices","authors":"Jin Qi, Shaoxia Li, Jun Su, Yushi Lu, Wenjin Yu, Changxia Li","doi":"10.1016/j.hpj.2025.02.006","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.02.006","url":null,"abstract":"N6-methyladenosine (m<ce:sup loc=\"post\">6</ce:sup>A) modification, the most abundant internal modification in messenger RNA (mRNA) and long non-coding RNA (lncRNA), has emerged as a critical epitranscriptomic regulatory mechanism in eukaryotes. While the importance of m<ce:sup loc=\"post\">6</ce:sup>A modification in various biological processes has been recognized, a comprehensive understanding of its diverse roles in plant biology and agricultural applications remains fragmented. This review analyzes recent advances in m<ce:sup loc=\"post\">6</ce:sup>A modification's biological functions in plants. m<ce:sup loc=\"post\">6</ce:sup>A modification plays crucial roles in multiple aspects of plant life, including seed germination, organ development, and reproductive structure formation. Furthermore, m<ce:sup loc=\"post\">6</ce:sup>A has been found to significantly influence plant responses to environmental stresses, including salt, drought, temperature, and heavy metal exposure. We also uncover m<ce:sup loc=\"post\">6</ce:sup>A involvement in important agricultural traits. This review provides insights into the mechanistic understanding of m<ce:sup loc=\"post\">6</ce:sup>A modification in plants and highlights its applications in agricultural improvement, offering a foundation for future research in crop enhancement and stress resistance.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"5 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatiotemporal and subgenome expression dynamics and regulatory divergence of the glucosinolate gene homologs in the allopolyploid Brassica juncea","authors":"Jinze Zhang, Shaomin Guo, Xu Yang, Lijing Xiao, Qingjing Ouyang, Hairun Jin, Xu Long, Zhongbin Yan, Entang Tian","doi":"10.1016/j.hpj.2025.02.007","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.02.007","url":null,"abstract":"Plant glucosinolates (GSLs) have attracted considerable attention due to their significant roles in plant defense mechanisms and cancer prevention. However, the gene expression characteristics and mechanisms underlying phenotypic variations in <ce:italic>Brassica juncea</ce:italic>, particularly in key vegetable and oilseed varieties, remain largely unexplored. This study examined the retention and the spatiotemporal expression and subgenome expression dynamics of duplicated genes predicted to regulate GSL metabolism in the allopolyploid <ce:italic>B. juncea</ce:italic>. Our findings reveal that homologs of <ce:italic>Arabidopsis</ce:italic> GSL genes (HAGG) are preferentially retained in the <ce:italic>B. juncea</ce:italic> genome compared to other genes. Most HAGG genes (76.5 %) were expressed across all tissues, while the remainders exhibited tissue-specific expression patterns. A majority of homoeologous pairs (54.09 %) demonstrated balanced expression levels, whereas the rest showed a pronounced A-genome dominance in leaves and stems. Notably, two <ce:italic>GRT2</ce:italic> homologs, <ce:italic>Bju.GTR2.A03</ce:italic> and <ce:italic>Bju.GTR2.B06</ce:italic>, which exhibit regulatory divergence in GSL accumulation, were identified. Co-dominant allele-specific markers developed for <ce:italic>Bju.GTR2.A03</ce:italic> and <ce:italic>Bju.GTR2.B06</ce:italic> colocalized with their respective quantitative trait loci (QTLs) and co-segregated with GSL content, corroborating the results of QTL mapping. Furthermore, GSL content was significantly correlated with resistance to <ce:italic>Sclerotinia sclerotiorum</ce:italic>. Predictions regarding the functions of HAGG genes further validated their functional divergence. This study provides a foundation for enhancing GSL content in whole plants or specific tissues, facilitating future applications in agriculture and cancer prevention in <ce:italic>B. juncea</ce:italic>.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"136 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome and metabolome analysis reveals regulatory network associated with light-independent anthocyanin biosynthesis in blueberry fruit","authors":"Xuyan Li, Shouwen Wang, Hongxue Li, Fangyun Qian, Haiyang Wang, Jingying Wang, Lulu Zhai, Baosheng Shi, Junwei Huo, Shaomin Bian","doi":"10.1016/j.hpj.2025.02.004","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.02.004","url":null,"abstract":"Anthocyanins are vital secondary metabolites contributing to fruit pigmentation and antioxidative properties. While light is a well-known regulator of anthocyanin biosynthesis, the molecular basis of light-independent anthocyanin accumulation remains underexplored. In this study, integrated analysis of metabolome and transcriptome showed that the anthocyanin content in blueberry (<ce:italic>Vaccinium corymbosum</ce:italic> ‘Bluetta’) fruit was slightly decreased by light-impermeable bagging treatment, while anthocyanin biosynthetic genes were transcriptionally inhibited to different levels, suggesting a slight influence of the bagging treatment on anthocyanin accumulation. Further observation showed that fruit bagging did not alter ethylene production but decreased ABA content. Noticeably, two <ce:italic>VcMYBA/MYB1</ce:italic>s were not transcriptionally altered by the light-impermeable bagging treatment. Consistently, histochemical GUS analysis and pharmacological manipulation suggested light-independent and ethylene-inducible expression of <ce:italic>VcMYBA/MYB1</ce:italic>. Moreover, WGCNA analysis revealed 3759 genes positively associated with <ce:italic>MYBA/MYB1</ce:italic> such as ethylene-associated genes, etc. Additionally, <ce:italic>VcbZIP55</ce:italic>s and <ce:italic>VcCOP1</ce:italic>s were activated and inactivated by the bagging treatment, respectively. These findings provided a framework of light-independent anthocyanin biosynthesis in blueberry fruit.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"24 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein post-translational modification in plants: Regulation and beyond","authors":"Zhenxiang Li, Liqian Qin, Dongjiao Wang, Tianzhen Cui, Yanlan Liang, Shoujian Zang, Tingting Sun, Wanying Zhao, Qibin Wu, Youxiong Que","doi":"10.1016/j.hpj.2025.02.003","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.02.003","url":null,"abstract":"Post-translational modification (PTM) of proteins is a crucial regulatory mechanism in plant cells, enabling rapid and purposeful regulation of their functions. Modified proteins play various roles in signaling pathways, including plant growth and development, plant metabolism, and the response to adversity stress. In recent years, there has been an increase in the number of studies focusing on plant PTM maps and functional analysis. Here we aim to review the PTM types in plants, especially in horticultural plants and tropical crops, the interactions between and among PTMs, and more importantly, the underlying pathways and functions. Additionally, the potential application of PTMs in breeding is briefly discussed. This paper focuses on plant PTMs and provides a foundation for further investigation into the functions and regulatory mechanisms of PTMs in plant proteins as well as for crop improvement.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"23 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide analyses of RWP–RK reveal a potential role for a key gene, VvNLP1.1, in the grapevine response to nitrate","authors":"Xin Yang, Jingwen Li, Guipeng Liu, Lu Bian, Mingxin Feng, Yujia Liu, Kai Li, Jiayin Shang, Yulin Fang, Tengfei Xu, Jiangfei Meng","doi":"10.1016/j.hpj.2025.02.002","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.02.002","url":null,"abstract":"Nitrate is the primary nitrogen source for plants and is a signaling molecule regulating various plant developmental processes. Despite its significance, limited information is available on nitrate signaling in <ce:italic>Vitis vinifera</ce:italic>. We identified nine <ce:italic>VvRWP–RK</ce:italic> genes distributed across eight chromosomes using genome–wide identification and evolutionary analyses. Among these, <ce:italic>VvNLP1–4</ce:italic> and <ce:italic>VvRKD1–5</ce:italic> are associated with nitrate signaling and reproductive growth, respectively. To investigate their potential functions, structures, <ce:italic>cis</ce:italic>–acting promoter elements, functional structural domains, phylogenetic trees, spatiotemporal expression levels in different tissues at different developmental stages, potential protein–protein interaction networks, synteny (gene content), collinearity (gene order), and three–dimensional protein structure prediction were explored. We found that long–term nitrate application dramatically promoted grapevine plantlet development, including primary root length and leaf growth, and <ce:italic>VvNLP1.1</ce:italic>, <ce:italic>VvNLP1.2</ce:italic>, and <ce:italic>VvNLP2</ce:italic> were highly expressed in ‘Thompson Seedless' root tissues under nitrate–enriched conditions. To clarify the critical role of nitrate in grapevine growth, we observed that nuclear localization of VvNLP1.1 increased significantly following nitrate treatment. VvNLP1.1 was found to bind to the promoter of the primary nitrate response gene <ce:italic>VvNRT1.</ce:italic>1, driving its transcriptional activity. These findings indicate that <ce:italic>VvNLP1.1</ce:italic> is a core transcription factor of the nitrate signaling pathway in grapevine. Nitrate molecular docking analysis revealed that VvNLP1.1 directly binds to nitrate ions, indicating its potential role as a nitrate sensor capable of directly perceiving nitrate concentration. We also discovered that short–term nitrate starvation impacts <ce:italic>VvNLP1.1</ce:italic> promoter activity, linked to the abscisic acid–binding element (ABRE) motif in its promoter region. Our results thus provide new insights into the molecular mechanisms underlying various physiological processes in grapevine, particularly the nitrate signaling pathway, and provide a theoretical basis for improving nitrogen use efficiency (NUE) in grapevine.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"75 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving detection of potato virus X resistance genes Rx1 and Rx2 in tetraploid potato using optimized PCR and KASP markers","authors":"Saiful Islam, Jiana Li, Jie Zheng, Yingtao Zuo, Ying Zou, Tao Yu, Mohammad Ataur Rahman, Xianzhou Nie, Zhiguo Fang, Tao Luo, Botao Song, Bihua Nie","doi":"10.1016/j.hpj.2025.01.014","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.014","url":null,"abstract":"Potato virus X (PVX) seriously threatens global potato cultivation, necessitating a deeper understanding of the genetic mechanisms responsible for resistance. The <ce:italic>Rx1</ce:italic> and <ce:italic>Rx2</ce:italic> genes play a pivotal role in breeding potatoes with broad-spectrum PVX resistance. However, current DNA markers for these genes present uncertainties, as they are found in both resistant and susceptible materials, complicating the identification of the responsible resistance gene. This study aimed to develop robust high-throughput molecular markers, specifically Kompetitive Allele-Specific PCR (KASP) markers, to reliably detect the <ce:italic>Rx1</ce:italic> and <ce:italic>Rx2</ce:italic> genes. Genetic polymorphism analysis of seven resistant and four susceptible potato genotypes revealed seven single nucleotide polymorphisms (SNPs) and one insertion-deletion (Indel), along with corresponding amino acid changes in the Rx1 and Rx2 protein sequences. Based on these variations, high-throughput molecular markers were developed. Notably, the primer pairs Rx1-1R-F + Rx1-5R-R and Rx2-1R2-F + Rx2-2R2-R showed high accuracy, detecting <ce:italic>Rx1</ce:italic> and <ce:italic>Rx2</ce:italic> genes without false positives or false negatives. Optimized multiplex PCR protocols also demonstrated strong potential in simultaneously detecting both genes. KASP markers targeting SNPs at positions 2 515 (G to C) in the <ce:italic>Rx1</ce:italic> CDS and 2 445 (C to G) in the <ce:italic>Rx2</ce:italic> CDS efficiently differentiated between heterozygous resistant and homozygous susceptible genotypes. Furthermore, these markers effectively identified <ce:italic>Rx1</ce:italic> and <ce:italic>Rx2</ce:italic> genotypes in 243 and 133 progenies across multiple crosses. Progenies that tested positive for <ce:italic>Rx1</ce:italic> and <ce:italic>Rx2</ce:italic> through PCR or KASP assays exhibited extreme resistance (ER) to PVX, while negative progeny was susceptible. These findings highlight the potential of the developed markers to accelerate the breeding cycle and improve the production of PVX-resistant potato varieties.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"26 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin modified root architecture system and accelerated mineral nutrient concentration of different pepper species under vanadium toxicity","authors":"Muhammad Ahsan Altaf, Huangying Shu, Naveed Mushtaq, Huizhen Fu, Xu Lu, Shanhan Cheng, Zhiwei Wang","doi":"10.1016/j.hpj.2024.12.006","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.12.006","url":null,"abstract":"Heavy metal contamination has adversely affected crop productivity around the world. Vanadium (V) toxicity is a significant environmental factor that lowers agricultural productivity by inhibiting plant development, diminishing nutrient uptake, and damaging root morphology. Melatonin (ME) is a well-known powerful antioxidant and multifunctional molecule that improves the resilience of plants against environmental stressors. In this work, we investigated the response of different pepper species (<ce:italic>Capsicum annuum</ce:italic> L. (CA), <ce:italic>C. baccatum</ce:italic> L. (CB), <ce:italic>C. pubescens</ce:italic> Ruiz &amp; Pav. (CP) to V toxicity as well as the possible roles of ME in enhancing V stress tolerance. Pepper roots were pretreated with ME (5 to μmol. L<ce:sup loc=\"post\">−1</ce:sup>) for three days, then V (30 mg. L<ce:sup loc=\"post\">−1</ce:sup>) was applied for 2 weeks as a stress treatment. The findings showed that plants pretreated with ME exposed to V stress had improved root activity and biomass production. The root morphology and mineral nutrient accumulation of the pepper species were greatly improved by ME application. In addition, ME treatments lowered leaf V concentrations by lowering V translocation from root to shoot. In a nutshell, ME treatment modified root architecture system, increased concentration of mineral nutrient, and reduced V accumulation of pepper species under V stress.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"240 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UV-B and mechanical wounding synergistically induce α-farnesene biosynthesis via CsHY5-CsMYC2 cooperation during oolong tea processing","authors":"Jingjie Cao, Dandan Li, Xiaohui Wang, Xin He, Ying Sun, Xin Cheng, Xiaochun Wan, Linlin Liu","doi":"10.1016/j.hpj.2025.01.015","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.015","url":null,"abstract":"UV-B application enhances the aroma quality of oolong tea; however, the underlying regulatory mechanism remains unclear. This study investigates the regulatory role of UV-B in the biosynthesis of <ce:italic>α</ce:italic>-farnesene, an important floral and fruity characteristic aroma. UV-B treatment significantly improved the aroma quality of ‘Foshou’ and ‘Yuquan’ oolong teas, increasing <ce:italic>α</ce:italic>-farnesene levels by 1.8- and 1.4-fold, respectively. The <ce:italic>α-farnesene synthase</ce:italic> (<ce:italic>CsAFS</ce:italic>), <ce:italic>ELONGATED HYPOCOTYL 5</ce:italic> (<ce:italic>CsHY5</ce:italic>), and <ce:italic>myelocytomatosis protein 2</ce:italic> (<ce:italic>CsMYC2</ce:italic>) exhibited a highly correlated expression pattern closely associated with <ce:italic>α</ce:italic>-farnesene accumulation. Single-factor treatment revealed that <ce:italic>CsAFS</ce:italic> expression was induced by both UV-B and mechanical wounding, with <ce:italic>CsHY5</ce:italic> predominantly responding to UV-B radiation, while <ce:italic>CsMYC2</ce:italic> primarily responded to tumbling-induced mechanical wounding signal. Transient suppression of CsHY5 in tea leaves reduced the expression of both <ce:italic>CsAFS</ce:italic> and <ce:italic>CsMYC2</ce:italic> whereas <ce:italic>CsMYC2</ce:italic> suppression decreased <ce:italic>CsAFS</ce:italic> expression. G-box motifs were identified in promoters of <ce:italic>CsMYC2</ce:italic> and <ce:italic>CsAFS</ce:italic>, and the dual-luciferase reporter assay (LUC) and electrophoretic mobility shift assays (EMSA) demonstrated direct binding functions of CsHY5 to <ce:italic>CsAFS</ce:italic> and <ce:italic>CsMYC2</ce:italic> promoters, as well as CsMYC2 to the <ce:italic>CsAFS</ce:italic> promoter<ce:italic>.</ce:italic> Based on sensory evaluation, odourant quantification, gene expression, and molecular functional analysis, we propose that UV-B radiation and tumbling-induced wounding signals synergistically regulate <ce:italic>α</ce:italic>-farnesene biosynthesis through a coordinated interaction of CsHY5 and CsMYC2 during oolong tea processing. These findings improve our understanding of flavour formation during oolong tea production and also provide novel insights into artificial light application in tea manufacturing.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"42 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of RNA m6A on the formation of multi-petalization in Magnolia wufengensis: Mechanistic insights and gene expression analysis","authors":"Cunjie Li, Huarong Li, Jiang Ma, Xiaoning Fan, Yanjun Cai, Liyuan Chen, Hanlin Zhou, Hongwei Liang, Chao Zhou, Faju Chen","doi":"10.1016/j.hpj.2025.01.011","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.011","url":null,"abstract":"<ce:italic>N</ce:italic><ce:sup loc=\"post\">6</ce:sup>-Methyladenosine (m<ce:sup loc=\"post\">6</ce:sup>A) is the most common modification in the transcriptome of biological RNA and plays roles that include maintaining the stability and transportation of mRNA, mRNA precursor shearing, polyadenylation, and the initiation of translation. With the improving understanding of RNA methylation, m<ce:sup loc=\"post\">6</ce:sup>A modification is known to play vital roles in plant development and growth. The multi-petalization of flowering plants has high ornamental and research value in horticultural landscapes. However, the mechanism of RNA methylation in flower formation in <ce:italic>Magnolia wufengensis</ce:italic>, a classical multi-petalizational plant, remains unclear. This study compared and analyzed RNA m<ce:sup loc=\"post\">6</ce:sup>A methylation and the transcriptome in floral buds of two varieties with large differences in tepal number at the early stage of development. It was found that the degree of RNA m<ce:sup loc=\"post\">6</ce:sup>A methylation and relative expression levels of <ce:italic>MawuAGL6-2</ce:italic>, <ce:italic>MawuPI-4</ce:italic>, and <ce:italic>MawuAGL9</ce:italic> in ‘Jiaodan’ with 36 tepals were significantly higher than those in ‘Jiaohong’ with 9 tepals during the development of floral organ primordia. Combined with quantitative real-time PCR, the expression levels of <ce:italic>MawuAGL6-2</ce:italic>, <ce:italic>MawuPI-4</ce:italic>, and <ce:italic>MawuAGL9</ce:italic> were positively correlated with the number of tepals. Transgenic experiments showed that <ce:italic>MawuAGL6-1/2</ce:italic>, and <ce:italic>MawuPI-4</ce:italic> can increase the number of petals in <ce:italic>Arabidopsis</ce:italic>. Moreover, <ce:italic>MawuAGL6-2</ce:italic> and <ce:italic>MawuPI-4</ce:italic> can restore the missing petal phenotype of mutant <ce:italic>Arabidopsis</ce:italic>. Yeast two hybrid and yeast three hybrid indicated that MawuAGL6-2, MawuAP3-1/2, and MawuPI-4 could interact with each other under the mediation of the class E protein MawuAGL9. Based on these results, it is hypothesized that m<ce:sup loc=\"post\">6</ce:sup>A methylation influences the multi-petalization of <ce:italic>Magnolia wufengensis</ce:italic> by affecting the expression levels of <ce:italic>MawuAGL6-2</ce:italic>, <ce:italic>MawuAP3-1/2</ce:italic>, <ce:italic>MawuPI-4</ce:italic>, and <ce:italic>MawuAGL9</ce:italic>. These findings provide a better understanding of the molecular mechanisms of epigenetic modifications in flower developmental diversity.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"90 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative metabolome and transcriptome revealed the CpODO1 transcription factor controlling the varied floral scent traits of three wintersweet cultivars","authors":"Yubing Yong, Qi Zhou, Xin Ding, Jianming Cheng, Chengcheng Zhou, Zhiguo Shen, Lin Zhang","doi":"10.1016/j.hpj.2025.01.010","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.010","url":null,"abstract":"Wintersweet (<ce:italic>Chimonanthus praecox</ce:italic> L.), a well-known fragrant flowering shrub, is extensively planted for ornamental purpose and production of floral essential oil. Although the tepal color of wintersweet varieties exhibits the most remarkable diversity, variations in the floral scent traits are also noticeable across different cultivars. In this study, the floral volatile organic compounds (VOCs) in three wintersweet cultivars, ‘Yanlingsuxin’, ‘Yuxiang’, and ‘Hongyun’ were detected via GC–MS coupled with OAV. The distinct floral aromas of the three cultivars were primarily attributed to benzyl alcohol (abundant in ‘Yuxiang’), linalool (abundant in ‘Yanlingsuxin’), (−)-γ-cadinene and eugenol (abundant in ‘Hongyun’). Integrated analyses of metabolome and transcriptome showed that an R2R3-MYB transcription factor, <ce:italic>CpODO1</ce:italic>, potentially have a crucial regulatory function in controlling the production of multiple aroma compounds. Overexpression of <ce:italic>CpODO1</ce:italic> can enhance the production of benzyl alcohol in transgenic tobacco flowers. Analysis of DAP-seq data, EMSA and dual-luciferase assay revealed that CpODO1 predominantly regulate the expression of <ce:italic>CpCYP71</ce:italic>, a cytochrome P450 gene encoding a key enzyme in the production of benzyl alcohol, and the transcriptional regulation of <ce:italic>CpODO1</ce:italic> is driven by CpEOBII. The identification of polymorphisms in the MYB binding <ce:italic>cis</ce:italic>-motifs of <ce:italic>CpCYP71</ce:italic> and <ce:italic>CpODO1</ce:italic> promoters revealed the regulatory mechanism underlying the varied synthesis of benzyl alcohol in three wintersweet cultivars. This study provides new anchor points for floral scent quality improvement breeding of wintersweet, and the profusion of wintersweet germplasm can serve as a material basis for developing various aroma products.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"49 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}