{"title":"RNA m6A对白玉兰多花瓣形成的影响:机制及基因表达分析","authors":"Cunjie Li, Huarong Li, Jiang Ma, Xiaoning Fan, Yanjun Cai, Liyuan Chen, Hanlin Zhou, Hongwei Liang, Chao Zhou, Faju Chen","doi":"10.1016/j.hpj.2025.01.011","DOIUrl":null,"url":null,"abstract":"<ce:italic>N</ce:italic><ce:sup loc=\"post\">6</ce:sup>-Methyladenosine (m<ce:sup loc=\"post\">6</ce:sup>A) is the most common modification in the transcriptome of biological RNA and plays roles that include maintaining the stability and transportation of mRNA, mRNA precursor shearing, polyadenylation, and the initiation of translation. With the improving understanding of RNA methylation, m<ce:sup loc=\"post\">6</ce:sup>A modification is known to play vital roles in plant development and growth. The multi-petalization of flowering plants has high ornamental and research value in horticultural landscapes. However, the mechanism of RNA methylation in flower formation in <ce:italic>Magnolia wufengensis</ce:italic>, a classical multi-petalizational plant, remains unclear. This study compared and analyzed RNA m<ce:sup loc=\"post\">6</ce:sup>A methylation and the transcriptome in floral buds of two varieties with large differences in tepal number at the early stage of development. It was found that the degree of RNA m<ce:sup loc=\"post\">6</ce:sup>A methylation and relative expression levels of <ce:italic>MawuAGL6-2</ce:italic>, <ce:italic>MawuPI-4</ce:italic>, and <ce:italic>MawuAGL9</ce:italic> in ‘Jiaodan’ with 36 tepals were significantly higher than those in ‘Jiaohong’ with 9 tepals during the development of floral organ primordia. Combined with quantitative real-time PCR, the expression levels of <ce:italic>MawuAGL6-2</ce:italic>, <ce:italic>MawuPI-4</ce:italic>, and <ce:italic>MawuAGL9</ce:italic> were positively correlated with the number of tepals. Transgenic experiments showed that <ce:italic>MawuAGL6-1/2</ce:italic>, and <ce:italic>MawuPI-4</ce:italic> can increase the number of petals in <ce:italic>Arabidopsis</ce:italic>. Moreover, <ce:italic>MawuAGL6-2</ce:italic> and <ce:italic>MawuPI-4</ce:italic> can restore the missing petal phenotype of mutant <ce:italic>Arabidopsis</ce:italic>. Yeast two hybrid and yeast three hybrid indicated that MawuAGL6-2, MawuAP3-1/2, and MawuPI-4 could interact with each other under the mediation of the class E protein MawuAGL9. Based on these results, it is hypothesized that m<ce:sup loc=\"post\">6</ce:sup>A methylation influences the multi-petalization of <ce:italic>Magnolia wufengensis</ce:italic> by affecting the expression levels of <ce:italic>MawuAGL6-2</ce:italic>, <ce:italic>MawuAP3-1/2</ce:italic>, <ce:italic>MawuPI-4</ce:italic>, and <ce:italic>MawuAGL9</ce:italic>. These findings provide a better understanding of the molecular mechanisms of epigenetic modifications in flower developmental diversity.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"90 1","pages":""},"PeriodicalIF":6.2000,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of RNA m6A on the formation of multi-petalization in Magnolia wufengensis: Mechanistic insights and gene expression analysis\",\"authors\":\"Cunjie Li, Huarong Li, Jiang Ma, Xiaoning Fan, Yanjun Cai, Liyuan Chen, Hanlin Zhou, Hongwei Liang, Chao Zhou, Faju Chen\",\"doi\":\"10.1016/j.hpj.2025.01.011\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<ce:italic>N</ce:italic><ce:sup loc=\\\"post\\\">6</ce:sup>-Methyladenosine (m<ce:sup loc=\\\"post\\\">6</ce:sup>A) is the most common modification in the transcriptome of biological RNA and plays roles that include maintaining the stability and transportation of mRNA, mRNA precursor shearing, polyadenylation, and the initiation of translation. With the improving understanding of RNA methylation, m<ce:sup loc=\\\"post\\\">6</ce:sup>A modification is known to play vital roles in plant development and growth. The multi-petalization of flowering plants has high ornamental and research value in horticultural landscapes. However, the mechanism of RNA methylation in flower formation in <ce:italic>Magnolia wufengensis</ce:italic>, a classical multi-petalizational plant, remains unclear. This study compared and analyzed RNA m<ce:sup loc=\\\"post\\\">6</ce:sup>A methylation and the transcriptome in floral buds of two varieties with large differences in tepal number at the early stage of development. It was found that the degree of RNA m<ce:sup loc=\\\"post\\\">6</ce:sup>A methylation and relative expression levels of <ce:italic>MawuAGL6-2</ce:italic>, <ce:italic>MawuPI-4</ce:italic>, and <ce:italic>MawuAGL9</ce:italic> in ‘Jiaodan’ with 36 tepals were significantly higher than those in ‘Jiaohong’ with 9 tepals during the development of floral organ primordia. Combined with quantitative real-time PCR, the expression levels of <ce:italic>MawuAGL6-2</ce:italic>, <ce:italic>MawuPI-4</ce:italic>, and <ce:italic>MawuAGL9</ce:italic> were positively correlated with the number of tepals. Transgenic experiments showed that <ce:italic>MawuAGL6-1/2</ce:italic>, and <ce:italic>MawuPI-4</ce:italic> can increase the number of petals in <ce:italic>Arabidopsis</ce:italic>. Moreover, <ce:italic>MawuAGL6-2</ce:italic> and <ce:italic>MawuPI-4</ce:italic> can restore the missing petal phenotype of mutant <ce:italic>Arabidopsis</ce:italic>. Yeast two hybrid and yeast three hybrid indicated that MawuAGL6-2, MawuAP3-1/2, and MawuPI-4 could interact with each other under the mediation of the class E protein MawuAGL9. Based on these results, it is hypothesized that m<ce:sup loc=\\\"post\\\">6</ce:sup>A methylation influences the multi-petalization of <ce:italic>Magnolia wufengensis</ce:italic> by affecting the expression levels of <ce:italic>MawuAGL6-2</ce:italic>, <ce:italic>MawuAP3-1/2</ce:italic>, <ce:italic>MawuPI-4</ce:italic>, and <ce:italic>MawuAGL9</ce:italic>. These findings provide a better understanding of the molecular mechanisms of epigenetic modifications in flower developmental diversity.\",\"PeriodicalId\":13178,\"journal\":{\"name\":\"Horticultural Plant Journal\",\"volume\":\"90 1\",\"pages\":\"\"},\"PeriodicalIF\":6.2000,\"publicationDate\":\"2025-03-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Horticultural Plant Journal\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1016/j.hpj.2025.01.011\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"HORTICULTURE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Horticultural Plant Journal","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.hpj.2025.01.011","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"HORTICULTURE","Score":null,"Total":0}
Effects of RNA m6A on the formation of multi-petalization in Magnolia wufengensis: Mechanistic insights and gene expression analysis
N6-Methyladenosine (m6A) is the most common modification in the transcriptome of biological RNA and plays roles that include maintaining the stability and transportation of mRNA, mRNA precursor shearing, polyadenylation, and the initiation of translation. With the improving understanding of RNA methylation, m6A modification is known to play vital roles in plant development and growth. The multi-petalization of flowering plants has high ornamental and research value in horticultural landscapes. However, the mechanism of RNA methylation in flower formation in Magnolia wufengensis, a classical multi-petalizational plant, remains unclear. This study compared and analyzed RNA m6A methylation and the transcriptome in floral buds of two varieties with large differences in tepal number at the early stage of development. It was found that the degree of RNA m6A methylation and relative expression levels of MawuAGL6-2, MawuPI-4, and MawuAGL9 in ‘Jiaodan’ with 36 tepals were significantly higher than those in ‘Jiaohong’ with 9 tepals during the development of floral organ primordia. Combined with quantitative real-time PCR, the expression levels of MawuAGL6-2, MawuPI-4, and MawuAGL9 were positively correlated with the number of tepals. Transgenic experiments showed that MawuAGL6-1/2, and MawuPI-4 can increase the number of petals in Arabidopsis. Moreover, MawuAGL6-2 and MawuPI-4 can restore the missing petal phenotype of mutant Arabidopsis. Yeast two hybrid and yeast three hybrid indicated that MawuAGL6-2, MawuAP3-1/2, and MawuPI-4 could interact with each other under the mediation of the class E protein MawuAGL9. Based on these results, it is hypothesized that m6A methylation influences the multi-petalization of Magnolia wufengensis by affecting the expression levels of MawuAGL6-2, MawuAP3-1/2, MawuPI-4, and MawuAGL9. These findings provide a better understanding of the molecular mechanisms of epigenetic modifications in flower developmental diversity.
期刊介绍:
Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.