Horticultural Plant Journal最新文献

{"title":"UV-B and mechanical wounding synergistically induce α-farnesene biosynthesis via CsHY5-CsMYC2 cooperation during oolong tea processing","authors":"Jingjie Cao, Dandan Li, Xiaohui Wang, Xin He, Ying Sun, Xin Cheng, Xiaochun Wan, Linlin Liu","doi":"10.1016/j.hpj.2025.01.015","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.015","url":null,"abstract":"UV-B application enhances the aroma quality of oolong tea; however, the underlying regulatory mechanism remains unclear. This study investigates the regulatory role of UV-B in the biosynthesis of <ce:italic>α</ce:italic>-farnesene, an important floral and fruity characteristic aroma. UV-B treatment significantly improved the aroma quality of ‘Foshou’ and ‘Yuquan’ oolong teas, increasing <ce:italic>α</ce:italic>-farnesene levels by 1.8- and 1.4-fold, respectively. The <ce:italic>α-farnesene synthase</ce:italic> (<ce:italic>CsAFS</ce:italic>), <ce:italic>ELONGATED HYPOCOTYL 5</ce:italic> (<ce:italic>CsHY5</ce:italic>), and <ce:italic>myelocytomatosis protein 2</ce:italic> (<ce:italic>CsMYC2</ce:italic>) exhibited a highly correlated expression pattern closely associated with <ce:italic>α</ce:italic>-farnesene accumulation. Single-factor treatment revealed that <ce:italic>CsAFS</ce:italic> expression was induced by both UV-B and mechanical wounding, with <ce:italic>CsHY5</ce:italic> predominantly responding to UV-B radiation, while <ce:italic>CsMYC2</ce:italic> primarily responded to tumbling-induced mechanical wounding signal. Transient suppression of CsHY5 in tea leaves reduced the expression of both <ce:italic>CsAFS</ce:italic> and <ce:italic>CsMYC2</ce:italic> whereas <ce:italic>CsMYC2</ce:italic> suppression decreased <ce:italic>CsAFS</ce:italic> expression. G-box motifs were identified in promoters of <ce:italic>CsMYC2</ce:italic> and <ce:italic>CsAFS</ce:italic>, and the dual-luciferase reporter assay (LUC) and electrophoretic mobility shift assays (EMSA) demonstrated direct binding functions of CsHY5 to <ce:italic>CsAFS</ce:italic> and <ce:italic>CsMYC2</ce:italic> promoters, as well as CsMYC2 to the <ce:italic>CsAFS</ce:italic> promoter<ce:italic>.</ce:italic> Based on sensory evaluation, odourant quantification, gene expression, and molecular functional analysis, we propose that UV-B radiation and tumbling-induced wounding signals synergistically regulate <ce:italic>α</ce:italic>-farnesene biosynthesis through a coordinated interaction of CsHY5 and CsMYC2 during oolong tea processing. These findings improve our understanding of flavour formation during oolong tea production and also provide novel insights into artificial light application in tea manufacturing.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"42 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The regulatory network composed of phytohormones, transcription factors and non-coding RNAs is involved in the flavonoids biosynthesis of fruits","authors":"Xiaoyuan Zheng, Xuejiao Zhang, Fankui Zeng","doi":"10.1016/j.hpj.2025.01.006","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.006","url":null,"abstract":"Flavonoids, abundant in the fruits, are pivotal to their growth, development, and storage. In addition, they have significant beneficial effects on human health. Consequently, research is increasingly concentrating on the regulatory mechanisms governing flavonoid biosynthesis in fruits. Phytohormones are involved in the regulation of flavonoid biosynthesis. The abscisic acid, ethylene, jasmonate, cytokinins, and brassinosteroids promote flavonoid biosynthesis, while auxin negatively regulates flavonoid biosynthesis. Subsequently, transcription factors from the MYB, bHLH, WRKY, NAC, and bZIP families are pivotal in regulating flavonoid biosynthesis. In addition, non-coding RNAs (microRNA and lncRNA) also participate in the regulation of flavonoids biosynthesis. MicroRNAs are generally believed to negatively regulate flavonoid metabolism in fruits, while lncRNAs have the opposite effect. Furthermore, the interactions between plant hormones, transcription factors, and non-coding RNAs in fruit flavonoid biosynthesis were analyzed. Ultimately, a foundational regulatory network for fruit flavonoid biosynthesis was hereby established.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"19 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VqBGH52 enhances the accumulation of trans-resveratrol through hydrolysis of trans-piceid and resistance to powdery mildew in Chinese wild grapevine","authors":"Zhi Li, Wenzhe Liu, Yuejin Wang","doi":"10.1016/j.hpj.2024.12.004","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.12.004","url":null,"abstract":"Powdery mildew poses a significant threat to grapevine cultivation worldwide, resulting in increased production costs. <ce:italic>Vitis quinquangularis</ce:italic> accession ‘Danfeng-2’ accumulates high concentrations of stilbenes and exhibits resistance to powdery mildew. This study investigates the regulatory mechanisms of stilbene accumulation and disease-resistance in ‘Danfeng-2’ and <ce:italic>V. vinifera</ce:italic> cultivar ‘Cabernet Sauvignon’ grapevine following inoculation with <ce:italic>Erysiphe necator</ce:italic>. ‘Danfeng-2’ accumulates high levels of stilbenes at 3 days post-inoculation (dpi), whereas ‘Cabernet Sauvignon’ reaches peak accumulation by 5 dpi. Transcriptomic analysis revealed the upregulation of the gene <ce:italic>VqBGH52</ce:italic> in ‘Danfeng-2’ specifically at 3 dpi, suggesting its role in disease resistance. The prokaryotically expressed VqBGH52 and VvBGH52 proteins efficiently hydrolyze <ce:italic>trans</ce:italic>-piceid into <ce:italic>trans</ce:italic>-resveratrol. These results are consistent with findings that genetically transformed lines overexpressing <ce:italic>VqBGH52</ce:italic> exhibit enhanced resistance to <ce:italic>E. necator</ce:italic>, along with increased levels of <ce:italic>trans</ce:italic>-resveratrol, ε-viniferin, and pterostilbene, and reduced levels of <ce:italic>trans</ce:italic>-piceid. To uncover the mechanisms of the distinct expression of <ce:italic>VqBGH52</ce:italic>, we characterized its promoter and found that it contains an additional 1492-bp fragment compared to the <ce:italic>VvBGH52</ce:italic> promoter. This fragment is associated with the rapid response of <ce:italic>VqBGH52</ce:italic> upon <ce:italic>E. necator</ce:italic> inoculation in ‘Danfeng-2’, as well as the induction of disease resistance genes and those responding to SA, flg22, and chitin treatments. In conclusion, VqBGH52 hydrolyzes <ce:italic>trans</ce:italic>-piceid to generate <ce:italic>trans</ce:italic>-resveratrol, which subsequently serves as a substrate for producing toxic stilbenes such as ε-viniferin and pterostilbene. These findings elucidate the metabolic mechanisms through which <ce:italic>VqBGH52</ce:italic> contributes to powdery mildew resistance by converting <ce:italic>trans</ce:italic>-piceid into toxic stilbenes.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"17 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PSI-mediated NADPH production and enzyme regulation are critical for carbon assimilation in pepper seedlings under cadmium stress","authors":"Zhimin Yu, Xinhao Huang, Jiaxuan Xiang, Xingxing Qin, Lili Xiang, Xuexiao Zou, Fan Zhu","doi":"10.1016/j.hpj.2025.01.005","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.005","url":null,"abstract":"Photosynthetic CO<ce:inf loc=\"post\">2</ce:inf> assimilation forms the basis of crop growth and yield formation and is highly sensitive to soil cadmium (Cd) pollution. This study investigated the mechanisms behind Cd-induced inhibition of photosynthetic CO<ce:inf loc=\"post\">2</ce:inf> assimilation in pepper (<ce:italic>Capsicum annuum</ce:italic> L.) seedlings under hydroponic conditions. Pepper plants were exposed to Cd at 0.3 and 1.0 mg L<ce:sup loc=\"post\">−1</ce:sup>, and their photosynthetic performance, photosystem I (PSI) and photosystem II (PSII) activity, Calvin-Benson-Bassham (CBB) cycle enzyme activities, ATP and NADPH content, and expression levels of photosynthesis-related genes were assessed. Results showed that Cd stress significantly reduced the activities of key CBB cycle enzymes, including Rubisco, phosphoglycerate kinase (PGK), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), along with the expression of their corresponding genes. Cd stress also caused severe PSI damage, disrupting electron transport, impairing NADPH synthesis, and triggering excessive reactive oxygen species (ROS) accumulation. These effects decreased the maximum Rubisco carboxylation rate (Vc<ce:inf loc=\"post\">max</ce:inf>) and ribulose-1,5-bisphosphate (RuBP) regeneration capacity (J<ce:inf loc=\"post\">max</ce:inf>), leading to a 35.2%–70.7 % reduction in the maximum CO<ce:inf loc=\"post\">2</ce:inf> assimilation rate (Pn<ce:inf loc=\"post\">max</ce:inf>). Variance partitioning analysis (VPA) identified PSI activity and NADPH content as the primary contributors to the Cd-induced decline in CO<ce:inf loc=\"post\">2</ce:inf> assimilation efficiency. Weighted gene co-expression network analysis (WGCNA) revealed strong positive correlations between the down-regulation of PSI structural genes (<ce:italic>PsaD</ce:italic>, <ce:italic>PsaE</ce:italic>, and <ce:italic>PsaF</ce:italic>) and electron transport genes (<ce:italic>PetE</ce:italic> and <ce:italic>PetF</ce:italic>) and the reduction in CO<ce:inf loc=\"post\">2</ce:inf> assimilation efficiency under Cd stress. In conclusion, this study emphasizes the critical role of PSI damage in Cd-induced disruption of photosynthetic CO<ce:inf loc=\"post\">2</ce:inf> assimilation in pepper seedlings and provides new insights into the physiological and molecular mechanisms underlying plant responses to Cd stress.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"69 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143641045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BjuBBX6-1 interacts with BjuNF-YB2/3 to regulate flowering time and drought tolerance in Brassica juncea","authors":"Xianjun Feng, Jingfei Li, Zhuoran Tang, Jiaxing Ge, Dayong Wei, Zhimin Wang, Qinglin Tang","doi":"10.1016/j.hpj.2025.01.003","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.003","url":null,"abstract":"An inappropriate flowering time and drought stress resistance in <ce:italic>Brassica juncea</ce:italic> (mustard) will decrease its yield and quality. B-box (BBX) transcription factors play an important role in the regulation of flowering and drought tolerance, but the biological functions and molecular mechanisms of the BBX family—especially BjuBBX6—remain largely unknown in <ce:italic>Brassica juncea</ce:italic>. In this study, we cloned the <ce:italic>BjuBBX6-1</ce:italic> gene from <ce:italic>Brassica juncea</ce:italic> and found that it was expressed mainly in flowers and leaves. BjuBBX6-1 was localized in the nucleus and had transcriptional activation activity. Overexpressing <ce:italic>BjuBBX6-1</ce:italic> caused earlier flowering compared with the wild type, while <ce:italic>BjuBBX6-1</ce:italic> silencing in RNAi lines led to later flowering. BjuBBX6-1 interacted with flowering factors BjuNF-YB2 and BjuNF-YB3 and subsequently bound to the promoters of its downstream genes to promote <ce:italic>BjuABI2</ce:italic> expression. However, it repressed <ce:italic>BjuFLC</ce:italic> and <ce:italic>BjuGLK</ce:italic> expression, resulting in earlier flowering. Under drought stress, overexpressing <ce:italic>BjuBBX6-1</ce:italic> plants reduced chlorophyll content and antioxidant enzyme activities, and increased malondialdehyde (MDA) content, while silencing <ce:italic>BjuBBX6-1</ce:italic> plants exhibited the opposite trend. The results indicate that BjuBBX6-1 negatively regulates drought tolerance in <ce:italic>Brassica juncea</ce:italic>. This study lays a theoretical foundation for in-depth research on the molecular mechanism of the BBX family in flowering regulation and drought tolerance breeding of <ce:italic>Brassica juncea</ce:italic>.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"11 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143641048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of CsRNF144 as a key gene regulating di-hydroxy catechins accumulation in Camellia tachangensis through genome-wide association studies","authors":"Yuting OuYang, Kaixin Rao, Limin He, Qinfei Song, Xiaojing Wang, Biao Xiong, Suzhen Niu","doi":"10.1016/j.hpj.2025.01.004","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.004","url":null,"abstract":"Tea is a widely consumed and nutritious beverage, rich in amino acids, caffeine, and catechins, which contribute to its taste and health-promoting properties. While much research has focused on catechins, which are essential for black tea quality, di-hydroxy catechins have received less attention. The genetic mechanisms that regulate the accumulation of these specific still need to be better understood, creating a gap in breeding strategies aimed at enhancing tea quality. This study conducted a genome-wide association study (GWAS) on 329 tea accessions from three species (<ce:italic>Camellia tachangensis</ce:italic> F. C. Zhang, <ce:italic>Camellia sinensis</ce:italic> var. <ce:italic>sinensis</ce:italic>, and <ce:italic>Camellia gymnogyna</ce:italic> Chang) to address this gap, identifying significant variation in di-hydroxy catechin content. <ce:italic>C. tachangensis</ce:italic> displayed notably higher di-hydroxy catechin levels, and 44 significant SNPs and 5 candidate genes were identified. Functional analysis, including overexpression in tobacco and antisense oligodeoxynucleotide (AsODN) experiments, showed that <ce:italic>CsRNF144</ce:italic> plays a critical role in this process. Overexpression of <ce:italic>CsRNF144</ce:italic> led to increased di-hydroxy catechin content, while AsODN interference in <ce:italic>C. tachangensis</ce:italic> significantly reduced di-hydroxy catechin levels, confirming the gene’s involvement in efficient accumulation. These findings identified a gene involved in the efficient accumulation of di-hydroxy catechins in <ce:italic>C. tachangensis</ce:italic> and provide a theoretical foundation for future tea breeding programs aimed at enhancing flavor. Additionally, the results emphasize the importance of protecting and developing wild tea accessions, which could serve as valuable genetic resources for improving cultivated varieties.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"55 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143641046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological, transcriptomic, and gene functional analyses of bulbil initiation and development of Lilium lancifolium","authors":"Xuemin Zhang, Jiahui Liang, Mingfang Zhang, Wenqiang Pan, Mengdi Wang, Zhiyi Yue, Zhixuan Zhang, Jian Wu, Xiuhai Zhang, Yunpeng Du","doi":"10.1016/j.hpj.2024.11.010","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.11.010","url":null,"abstract":"The bulbil, originating in the leaf axil of <ce:italic>Lilium lancifolium</ce:italic>, functions as a vital reproductive organ for bulbous propagation. The mechanism of bulbil formation, however, is still unclear. In this study, we conducted histological, transcriptomic, and gene function analyses on leaf axil samples throughout bulbil formation. Histological analysis revealed that the bulbils arose from the axillary meristem and bulbil formation undergoes a two-step process: initiation and development. During the bulbil initiation stage, RNA-seq analysis revealed that the differentially expressed genes (DEGs) were primarily enriched in phytohormone-related pathways, especially auxin. Through virus-induced gene silencing (VIGS), the individual silencing phenotypes of nine genes derived from four hormones imply that decreased auxin and ethylene signaling, paired with increased cytokinin and gibberellin, may contribute to bulbil initiation. Among the numerous differentially expressed transcription factors, <ce:italic>LlMYB119</ce:italic> may play a role as a candidate gene in auxin-regulated bulbil initiation, as confirmed by quantitative real-time PCR (qRT-PCR) and VIGS. During bulbil development, DEGs showed significant enrichment in carbohydrate metabolism, as well as phytohormone signal transduction. Silencing seven specific genes involved in auxin and ethylene signaling, cytokinin and gibberellin biosynthesis, as well as carbohydrate metabolism, resulted in inhibition of axillary organ development. In summary, this study offers a rich pool of candidate genes, enhancing our understanding of the regulatory mechanism underlying bulbil initiation and development, and holding significant commercial potential for the advancement of new reproductive organs in <ce:italic>L. lancifolium</ce:italic>.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"32 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143641047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AaEIL2 and AaERF059 are involved in fruit coloration and ripening by crossly regulating ethylene and auxin signal pathway in Actinidia arguta","authors":"Yukuo Li, Zhe Song, Xu Zhan, Miaomiao Lin, Xiaohan Li, Ran Wang, Leiming Sun, Hong Gu, Feng Wei, Jinbao Fang, Xiujuan Qi","doi":"10.1016/j.hpj.2024.07.013","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.07.013","url":null,"abstract":"In kiwifruit (<ce:italic>Actinidia arguta</ce:italic>), fruit coloration is typically accompanied by ripening; however, the intrinsic connection between these two processes remains unclear. In this study, we found that ethylene and auxin accelerated and suppressed fruit coloration and ripening, respectively, in <ce:italic>A. arguta</ce:italic>. ETHYLENE INSENSITIVE 3-LIKE 2 (AaEIL2) and ETHYLENE RESPONSIVE FACTOR (AaERF059) were mined and identified using yeast two-hybrid library screening and transcriptome analysis. AaEIL2, specifically induced to high expression by ethylene, was confirmed as a transcription factor that positively regulates coloration and ripening by targeting <ce:italic>AaLDOX</ce:italic> (leucoanthocyanidin dioxygenase involved in anthocyanin biosynthesis) and <ce:italic>AaPG18</ce:italic> (polygalacturonase involved in cell wall degradation) based on its subcellular localization in <ce:italic>Arabidopsis</ce:italic> protoplasts, stable genetic transformation in transgenic tomato, and yeast one-hybrid and luciferase activity assays. AaERF059 also responds to ethylene and regulates ethylene-/auxin-mediated fruit coloration and ripening by targeting the downstream genes <ce:italic>AaACS2</ce:italic> (ACC synthase, which is involved in ethylene biosynthesis) and <ce:italic>AaGH3</ce:italic> (Gretchen-Hagen 3, which is involved in the auxin pathway). Overall, AaEIL2 and AaERF059 regulate ethylene-and auxin-mediated fruit coloration and ripening by maintaining a dynamic balance in a positive and negative regulatory manner. Our results not only identified key genes but also established an intrinsic connection between fruit coloration and ripening in <ce:italic>A. arguta</ce:italic>.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"12 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MADS-box BSISTER transcription factors up-regulate proanthocyanidin biosynthesis in grapevine","authors":"Yujin Tang, Ling Wang, Congbo Huang, Ting Zhao, Yan Li, Chaohong Zhang","doi":"10.1016/j.hpj.2024.11.009","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.11.009","url":null,"abstract":"Proanthocyanidins (PAs) are a group of oligomeric flavonoids also known as condensed tannins. PAs in human diet have antioxidant and health-promoting effects. BSISTER (BS) promotes the accumulation of PAs, but its direct target gene and regulation mechanism in PAs biosynthesis are not clear. Here, we reported <ce:italic>VviBS1</ce:italic> and <ce:italic>VviBS2</ce:italic> genes, which bind directly to the promoter of PA synthesis key genes <ce:italic>VviANR</ce:italic> (<ce:italic>ANTHOCYANIDIN REDUCTASE</ce:italic>) and <ce:italic>VviLAR</ce:italic> (<ce:italic>LEUCOANTHOCYANIDIN REDUCTASE</ce:italic>) and up-regulate their expression to promote the PAs biosynthesis in grapevine. <ce:italic>VviBS1</ce:italic> and <ce:italic>VviBS2</ce:italic> partially rescued the transparent testa phenotype of an <ce:italic>Arabidopsis tt16</ce:italic> mutant. Overexpression of <ce:italic>BS1</ce:italic> and <ce:italic>BS2</ce:italic> in grapevine callus increased the content of PAs, as well as the expression of specific genes related to PA synthesis. VviBS1 and VviBS2 proteins bound directly to the promoter regions of the key PA synthesis genes <ce:italic>VviANR1</ce:italic>, <ce:italic>VviANR2</ce:italic>, and <ce:italic>VviLAR1</ce:italic>. Overexpression of <ce:italic>VviBS1</ce:italic> and <ce:italic>VviBS2</ce:italic> promotes <ce:italic>VviANR1</ce:italic>, <ce:italic>VviANR2</ce:italic> and <ce:italic>VviLAR1</ce:italic> expression and PAs biosynthesis. Some BS1- and BS2-interacting MADS-box proteins have an effect on PAs biosynthesis. This study provides insight into the regulatory mechanisms of PAs biosynthesis in grapevine, which could be effectively employed for metabolic engineering to increase PA content.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"33 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GWAS reveals the genetic basis and genomic regions underlying four active compounds in chrysanthemum","authors":"Xuefeng Zhang, Xinyi Ning, Yuhua He, Jiangshuo Su, Shiyun Wen, Zhaowen Lu, Wei Sun, Haibin Wang, Zhiyong Guan, Weimin Fang, Fadi Chen, Fei Zhang","doi":"10.1016/j.hpj.2024.07.012","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.07.012","url":null,"abstract":"Chrysanthemum is rich in active compounds such as flavonoids and phenolic acids, and its dried head flowers are commonly used for tea and medicinal purposes. However, the genetic determinism underlying chrysanthemum active compounds remains elusive. In this study, we evaluated a panel of 137 chrysanthemum accessions for total flavonoids, chlorogenic acid, luteolin, and isochlorogenic acid A across two consecutive years. The four active compounds exhibited considerable variation, with a coefficient of variation ranging from 44.96 % to 76.30 %. Significant differences were observed in genotype and environments, and the broad-sense heritability was estimated at 0.5–0.63 for all examined traits. Significant pair-wise correlation was found between the four active compounds. Several accessions showing the highest active compounds were figured out for breeding use by integrating the membership function and hierarchical cluster analysis methods. Based on the 327 042 high-quality SNPs, a genome-wide association study (GWAS) captured 59 significant SNPs for the four active compounds, of which 24 elite alleles exhibited pyramiding effects. A total of 18 potential candidate genes were mined, among which <ce:italic>evm.model.scaffold_1149.273</ce:italic> (<ce:italic>QUA1</ce:italic>) has one linkage disequilibrium (LD) block corresponding to Hap4 with the highest luteolin content. The findings are beneficial to understanding the genetic basis of the active compounds and provide parental materials and valuable markers for the genetic improvement of active compounds in chrysanthemum.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"19 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}