Horticultural Plant Journal最新文献

{"title":"AaEIL2 and AaERF059 are involved in fruit coloration and ripening by crossly regulating ethylene and auxin signal pathway in Actinidia arguta","authors":"Yukuo Li, Zhe Song, Xu Zhan, Miaomiao Lin, Xiaohan Li, Ran Wang, Leiming Sun, Hong Gu, Feng Wei, Jinbao Fang, Xiujuan Qi","doi":"10.1016/j.hpj.2024.07.013","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.07.013","url":null,"abstract":"In kiwifruit (<ce:italic>Actinidia arguta</ce:italic>), fruit coloration is typically accompanied by ripening; however, the intrinsic connection between these two processes remains unclear. In this study, we found that ethylene and auxin accelerated and suppressed fruit coloration and ripening, respectively, in <ce:italic>A. arguta</ce:italic>. ETHYLENE INSENSITIVE 3-LIKE 2 (AaEIL2) and ETHYLENE RESPONSIVE FACTOR (AaERF059) were mined and identified using yeast two-hybrid library screening and transcriptome analysis. AaEIL2, specifically induced to high expression by ethylene, was confirmed as a transcription factor that positively regulates coloration and ripening by targeting <ce:italic>AaLDOX</ce:italic> (leucoanthocyanidin dioxygenase involved in anthocyanin biosynthesis) and <ce:italic>AaPG18</ce:italic> (polygalacturonase involved in cell wall degradation) based on its subcellular localization in <ce:italic>Arabidopsis</ce:italic> protoplasts, stable genetic transformation in transgenic tomato, and yeast one-hybrid and luciferase activity assays. AaERF059 also responds to ethylene and regulates ethylene-/auxin-mediated fruit coloration and ripening by targeting the downstream genes <ce:italic>AaACS2</ce:italic> (ACC synthase, which is involved in ethylene biosynthesis) and <ce:italic>AaGH3</ce:italic> (Gretchen-Hagen 3, which is involved in the auxin pathway). Overall, AaEIL2 and AaERF059 regulate ethylene-and auxin-mediated fruit coloration and ripening by maintaining a dynamic balance in a positive and negative regulatory manner. Our results not only identified key genes but also established an intrinsic connection between fruit coloration and ripening in <ce:italic>A. arguta</ce:italic>.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"12 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MADS-box BSISTER transcription factors up-regulate proanthocyanidin biosynthesis in grapevine","authors":"Yujin Tang, Ling Wang, Congbo Huang, Ting Zhao, Yan Li, Chaohong Zhang","doi":"10.1016/j.hpj.2024.11.009","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.11.009","url":null,"abstract":"Proanthocyanidins (PAs) are a group of oligomeric flavonoids also known as condensed tannins. PAs in human diet have antioxidant and health-promoting effects. BSISTER (BS) promotes the accumulation of PAs, but its direct target gene and regulation mechanism in PAs biosynthesis are not clear. Here, we reported <ce:italic>VviBS1</ce:italic> and <ce:italic>VviBS2</ce:italic> genes, which bind directly to the promoter of PA synthesis key genes <ce:italic>VviANR</ce:italic> (<ce:italic>ANTHOCYANIDIN REDUCTASE</ce:italic>) and <ce:italic>VviLAR</ce:italic> (<ce:italic>LEUCOANTHOCYANIDIN REDUCTASE</ce:italic>) and up-regulate their expression to promote the PAs biosynthesis in grapevine. <ce:italic>VviBS1</ce:italic> and <ce:italic>VviBS2</ce:italic> partially rescued the transparent testa phenotype of an <ce:italic>Arabidopsis tt16</ce:italic> mutant. Overexpression of <ce:italic>BS1</ce:italic> and <ce:italic>BS2</ce:italic> in grapevine callus increased the content of PAs, as well as the expression of specific genes related to PA synthesis. VviBS1 and VviBS2 proteins bound directly to the promoter regions of the key PA synthesis genes <ce:italic>VviANR1</ce:italic>, <ce:italic>VviANR2</ce:italic>, and <ce:italic>VviLAR1</ce:italic>. Overexpression of <ce:italic>VviBS1</ce:italic> and <ce:italic>VviBS2</ce:italic> promotes <ce:italic>VviANR1</ce:italic>, <ce:italic>VviANR2</ce:italic> and <ce:italic>VviLAR1</ce:italic> expression and PAs biosynthesis. Some BS1- and BS2-interacting MADS-box proteins have an effect on PAs biosynthesis. This study provides insight into the regulatory mechanisms of PAs biosynthesis in grapevine, which could be effectively employed for metabolic engineering to increase PA content.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"33 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GWAS reveals the genetic basis and genomic regions underlying four active compounds in chrysanthemum","authors":"Xuefeng Zhang, Xinyi Ning, Yuhua He, Jiangshuo Su, Shiyun Wen, Zhaowen Lu, Wei Sun, Haibin Wang, Zhiyong Guan, Weimin Fang, Fadi Chen, Fei Zhang","doi":"10.1016/j.hpj.2024.07.012","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.07.012","url":null,"abstract":"Chrysanthemum is rich in active compounds such as flavonoids and phenolic acids, and its dried head flowers are commonly used for tea and medicinal purposes. However, the genetic determinism underlying chrysanthemum active compounds remains elusive. In this study, we evaluated a panel of 137 chrysanthemum accessions for total flavonoids, chlorogenic acid, luteolin, and isochlorogenic acid A across two consecutive years. The four active compounds exhibited considerable variation, with a coefficient of variation ranging from 44.96 % to 76.30 %. Significant differences were observed in genotype and environments, and the broad-sense heritability was estimated at 0.5–0.63 for all examined traits. Significant pair-wise correlation was found between the four active compounds. Several accessions showing the highest active compounds were figured out for breeding use by integrating the membership function and hierarchical cluster analysis methods. Based on the 327 042 high-quality SNPs, a genome-wide association study (GWAS) captured 59 significant SNPs for the four active compounds, of which 24 elite alleles exhibited pyramiding effects. A total of 18 potential candidate genes were mined, among which <ce:italic>evm.model.scaffold_1149.273</ce:italic> (<ce:italic>QUA1</ce:italic>) has one linkage disequilibrium (LD) block corresponding to Hap4 with the highest luteolin content. The findings are beneficial to understanding the genetic basis of the active compounds and provide parental materials and valuable markers for the genetic improvement of active compounds in chrysanthemum.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"19 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The CsTCP13-CsKNAT1-CsKNAT6 module regulates leaf width through influencing gibberellin biosynthesis in citrus","authors":"Donghai Liu, Lele Chu, Haiqiang Liu, Shariq Mahmood Alam, Yongzhong Liu","doi":"10.1016/j.hpj.2024.12.002","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.12.002","url":null,"abstract":"Leaf morphology is an important agronomic trait that affects photosynthetic efficiency and plant architecture. Some KNOXI (Class I KNOTTED1-LIKE HOMEOBOX) or CIN-TCP (CINCINNATA-LIKE TEOSINTE BRANCHED1, CYCLOIDEA, and PROLIFERATING CELL FACTORS) transcription factors control leaf development by influencing GA (gibberellin) content. However, the knowledge about their interaction in regulating leaf morphogenesis is still scarce, especially in woody fruit plants such as citrus. In this study, we found that the expression of <ce:italic>CsTCP13</ce:italic> (a citrus <ce:italic>CIN-TCP</ce:italic> gene) dramatically decreased with a decreasing leaf length-width ratio in citrus. Transiently silencing <ce:italic>CsTCP13</ce:italic> in citrus produced wider leaves and significantly decreased <ce:italic>CsGA20ox1</ce:italic> (a key citrus <ce:italic>GA20-oxidase</ce:italic> gene for GA biosynthesis) expression and GAs (GA<ce:inf loc=\"post\">3</ce:inf>, GA<ce:inf loc=\"post\">4</ce:inf>, and GA<ce:inf loc=\"post\">7</ce:inf>) contents. Conversely, stable overexpression of <ce:italic>CsTCP13</ce:italic> in tobacco generated narrower leaves and significantly increased <ce:italic>GA20ox1</ce:italic> expression and GA<ce:inf loc=\"post\">1</ce:inf> content; it also significantly shortened the distance between veins and reduced the number of epidermal cells per unit area of leaf. Moreover, transactivation assays showed that CsTCP13 had transcriptional activation, and yeast two-hybrid, split-luciferase complementation, and bimolecular fluorescence complementation assays confirmed that CsTCP13 interacted with CsKNAT1 (a citrus KNOXI protein). Furthermore, yeast one-hybrid and dual-luciferase assays validated that CsTCP13 promoted while CsKNAT1 and its interacting protein CsKNAT6 inhibited <ce:italic>CsGA20ox1</ce:italic> expression by binding to its promoter. Interestingly, CsKNAT1 or the CsKNAT1-CsKNAT6 complex interacted with CsTCP13 to attenuate its promotion effect on <ce:italic>CsGA20ox1</ce:italic> expression. Taken together, our findings revealed a novel regulatory mechanism that CsTCP13 regulates citrus leaf width through directly influencing <ce:italic>CsGA20ox1</ce:italic> expression and then GA content, which can be negatively affected by the interaction with CsKNAT1 or the CsKNAT1-CsKNAT6 complex.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"29 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevated expression of CsCit and CsALMT9-like plays a key role in lowering citrate and increasing malate accumulation in Citrus tamurana × natsudaidai ‘Haruka’ fruit","authors":"Shariq Mahmood Alam, Donghai Liu, Yin Luo, Muhammad Abbas Khan, Han Han, Fatima Zaman, Muhammad Ateeq, Yongzhong Liu","doi":"10.1016/j.hpj.2024.12.003","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.12.003","url":null,"abstract":"Organic acid composition has a major influence on fruit taste and quality. In general, the fruits of common citrus varieties accumulate more citrate than malate. Here, we report that a citrus variety, ‘Haruka’ (<ce:italic>Citrus tamurana</ce:italic> × <ce:italic>natsudaidai</ce:italic>), accumulates more malate than citrate at the ripening stage. However, the underlying mechanism is unclear. Organic acid profiles were compared between ‘Haruka’ fruits and a common Ponkan cultivar, ‘Huagan 2’ (<ce:italic>Citrus reticulata</ce:italic>), during fruit development and ripening. We found that ‘Haruka’ fruit accumulated 75 % less citrate than ‘Huagan 2’ fruit and that the malate content was nearly 3-fold greater in ‘Haruka’ fruit than in ‘Huagan 2’ fruit. In addition, 12 aluminum-activated malate transporter (<ce:italic>ALMT</ce:italic>) genes were identified in the citrus genome, with <ce:italic>CsALMT9-like</ce:italic> being predominantly expressed in ‘Haruka’ fruit juice sacs. An analysis of genes associated with the accumulation of malate revealed that the expression levels of genes encoding a P-type proton pump (<ce:italic>CsPH8</ce:italic>), a citrate/H<ce:sup loc=\"post\">+</ce:sup> symporter (<ce:italic>CsCit</ce:italic>), and <ce:italic>CsALMT9-like</ce:italic> were significantly greater in ‘Haruka’ fruit than in ‘Huagan 2’ fruit. Moreover, <ce:italic>CsCit</ce:italic> overexpression significantly decreased the citrate content, whereas overexpressing and silencing <ce:italic>CsALMT9-like</ce:italic> significantly increased and decreased the malate content. In addition, shading significantly increased the expression of <ce:italic>CsPH8</ce:italic> but reduced the expression of <ce:italic>CsCit</ce:italic> and <ce:italic>CsALMT9-like</ce:italic> and significantly increased the citrate content but decreased the malate content, further confirming that <ce:italic>CsCit</ce:italic> and <ce:italic>CsALMT9-like</ce:italic> regulate the accumulation of citrate and malate in ‘Haruka’ fruit, respectively. Taken together, these data indicate that the relatively low citrate content of ‘Haruka’ is mainly due to increases in <ce:italic>CsCit</ce:italic> expression and that the relatively high malate content of ‘Haruka’ is mainly due to increases in <ce:italic>CsALMT9</ce:italic>-<ce:italic>like</ce:italic> expression. This study provides insight into the mechanisms that influence malate content in citrus fruit.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"1 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic and morpho-physiological attributes of drought resistance in Capsicum accessions","authors":"Kyu Kyu Thin, Soobin Lee, Je Min Lee","doi":"10.1016/j.hpj.2024.11.008","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.11.008","url":null,"abstract":"Drought stress is one of the factors limiting pepper production in water-stressed regions. It affects growth and development by inducing morphological, biochemical, and physiological changes. Breeding drought-resistant varieties is a sustainable strategy to mitigate drought, therefore, reliable evaluation systems are essential to identify drought-resistant pepper accessions. In this study, 100 pepper accessions were screened for drought resistance under highly controlled conditions at the vegetative stage. Selected accessions exhibited a significantly higher recovery rate after water-deficiency and showed durable resistance under greenhouse conditions. Correlation analysis between drought resistance with morphological and physiological traits showed that the recovery rate was positively correlated with root length and relative water content, and negatively correlated with plant height and leaf area. Gene expression analysis showed that the drought-resistant accession exhibited higher expression levels of drought-responsive genes under drought stress. Among the accessions, anthocyanin-accumulating peppers showed more significant drought resistance compared to other accessions. When the MYB transcription factor <ce:italic>An2</ce:italic>, the genetic determinant of anthocyanin accumulation, was silenced, drought resistance was significantly reduced. Drought-resistant accessions with favorable adaptive traits identified in this study will be valuable in various breeding programs to generate new pepper cultivars to cope with climate change.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"29 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol efficiency and the functional mechanisms of Bacillus velezensis RT-30 against kiwifruit soft rot","authors":"Wenli Yue, Junru Xu, Zitao Lv, Chen Zhou, Shunyuan Wu, Yunliu Zeng, Pu Liu","doi":"10.1016/j.hpj.2024.09.009","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.09.009","url":null,"abstract":"Soft rot is a severe postharvest disease affecting kiwifruit production. The management of this disease primarily relies on the use of chemical fungicides. However, biological control using antagonistic microorganisms presents a promising alternative to fungicides. In the present study, whole-genome sequencing identified and characterized <ce:italic>Bacillus velezensis</ce:italic> RT-30, as a biocontrol strain. The antiSMASH (antibiotics &amp; Secondary Metabolite Analysis Shell) analysis was employed to predict the possible secondary metabolites produced by strain RT-30. <ce:italic>In vitro</ce:italic> tests demonstrated that RT-30 inhibited the growth of both <ce:italic>Botryosphaeria dothidea</ce:italic> and <ce:italic>Diaporthe phragmitis</ce:italic>, while <ce:italic>in vivo</ce:italic> tests using <ce:italic>Actinidia chinensis</ce:italic> var. <ce:italic>deliciosa</ce:italic> ʻXuxiangʼ and ʻCuixiangʼ varieties also revealed a significant control effect. Molecular sequence analysis and Ultra-high pressure liquid chromatography-mass spectrometry (UPLC-MS) analysis of the fermentation filtrate of RT-30 indicated that the biocontrol strain primarily secreted three types of antimicrobial substances: macrolactin, bacillaene, and mycosubtilin. Furthermore, we explored the cultivation conditions of RT-30, recognizing the potential of this versatile bacterium for applications beyond antimicrobial production. This study provides a valuable biological tool for the management of kiwifruit soft rot and deepens our understanding of the biocontrol mechanism of <ce:italic>B</ce:italic>. <ce:italic>velezensis</ce:italic>.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"1 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143477817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Auxin promotes cucumber resistance to high ambient temperature by enhancing photosystem and activating DNA repair pathways","authors":"Bingwei Yu, Yujia Lin, Yuwei Gan, Chenyu Yang, Yonggui Liang, Qiteng Qin, Liping Chen, Renjian Liu, Hongbo Zhao, Zhengkun Qiu, Bihao Cao, Shuangshuang Yan","doi":"10.1016/j.hpj.2024.12.001","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.12.001","url":null,"abstract":"Global warming poses a huge challenge for plants to withstand increasing ambient temperatures. Cucumber is a kind of worldwide vegetable crop which is very sensitive to high temperature (HT). The mechanism of auxin affecting plant resistance to high temperature is still unclear. This study showed the relationship between auxin and heat resistance of cucumber. Here, we found that exogenous auxin improved cucumber heat tolerance by promoting root activity and mitigating the accumulation of reactive oxygen species (ROS) at the cotyledon stage. Additionally, exogenous auxin delayed internode shortening and leaf senescence under HT at three true-leaf stage. Exogenous auxin maintains photosystem Ⅱ stability in the thermotolerant cucumber plants. Transcriptome results indicated that photosynthesis and DNA repair processes were activated by exogenous 2,4-dichlorophenoxyacetic acid (2,4-D). Moreover, glutathione metabolism and carotenoid biosynthesis process were involved in ROS scavenging after the 2,4-D treatment under HT. In addition, overexpression of auxin biosynthesis gene CsYUC10b elevated cucumber resistance to high temperatures and enhanced the photosystem activity and DNA repair ability. Thus, auxin promoted heat resistance in cucumber by enhancing photosystem II and activating DNA repair pathways.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"32 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FRUITFULL2 controls tomato fertility through style length and pollen quality","authors":"Xiaowei Wang, Monica Lanzoni Rossi, Adriana Pinheiro Martinelli, Gerco C. Angenent, Ruud A. de Maagd","doi":"10.1016/j.hpj.2025.01.002","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.002","url":null,"abstract":"Tomato reproductive success and yield are particularly vulnerable to the negative effect of heat stress leading to stigma exsertion (protrusion) and lower pollen viability, both interfering with fertilization. Thus, understanding the regulation of these two traits in tomato is crucial for the yield and quality of the crop. Here, we found that knocking out the tomato MADS-domain transcription factor <ce:italic>FRUITFULL2 (FUL2)</ce:italic> function leads to a higher incidence of parthenocarpy in tomato. This phenotype was primarily due to impeded self-pollination as a consequence of the higher frequency of stigma exsertion and lower fertilization rates due to reduced pollen quality. Stigma exsertion in <ce:italic>ful2</ce:italic> mutants, in contrast to heat stress-induced exsertion, was caused by style elongation, particularly in the younger flowers of a truss. Interestingly, Quantitative Trait Loci for style elongation, stigma exsertion, and pollen viability map close to the position of <ce:italic>FUL2</ce:italic> on chromosome 3, making it a candidate gene underlying these QTLs. At the molecular level, <ce:italic>ful2</ce:italic> mutant styles have higher expression of <ce:italic>Style2.1</ce:italic> and <ce:italic>SE3.1</ce:italic>, which are known as positive regulators of style length. In addition, after reducing the impact of style exsertion and low pollen quality by manual pollination with wild-type pollen, <ce:italic>ful2</ce:italic> mutants exhibited reduced fruit size independent of seed number. This study reveals the contributions of flower number, style length, and pollen quality, as affected by FUL2, to tomato fertility and fruit size.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"52 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143477815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, identification and pathogenicity of two root rot pathogens Fusarium solani in Citrus","authors":"Tao Zhu, Xuzhao Luo, Chenxing Hao, Zhimei Zhu, Lian Liu, Ziniu Deng, Yunlin Cao, Xianfeng Ma","doi":"10.1016/j.hpj.2024.09.008","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.09.008","url":null,"abstract":"Root rot is a prevalent soil-borne fungal disease in citrus. Citron C-05 (<ce:italic>Citrus medica</ce:italic>) stands out as a germplasm within <ce:italic>Citrus</ce:italic> spp. due to its complete resistance to citrus canker and favorable characteristics such as single embryo and easy rooting. However, Citron C-05 was found to be highly susceptible to root rot during cultivation, with the specific pathogens previously unknown. In this study, four candidate fungal species were isolated from Citron C-05 roots. Sequence analysis of ITS, <ce:italic>EF-1α</ce:italic>, <ce:italic>RPB1</ce:italic>, and <ce:italic>RPB2</ce:italic> identified two <ce:italic>Fusarium solani</ce:italic> strains, Rr-2 and Rr-4, as the candidates causing root rot in Citron C-05. Resistance tests showed these two pathogens increased root damage rate from 10.30 % to 35.69 % in Citron C-05, sour orange (<ce:italic>Citrus aurantium</ce:italic>), sweet orange (<ce:italic>Citrus sinensis</ce:italic>) and pummelo (<ce:italic>Citrus grandis</ce:italic>). <ce:italic>F. solani</ce:italic> exhibited the weak pathogenicity towards trifoliate orange (<ce:italic>Poncirus trifoliata</ce:italic>). DAB staining revealed none of reddish-brown precipitation in the four susceptible citrus germplasm after infection with <ce:italic>F. solani</ce:italic>, while trifoliate orange exhibited significant H<ce:inf loc=\"post\">2</ce:inf>O<ce:inf loc=\"post\">2</ce:inf> accumulation. Trypan blue staining indicated increased cell death in the four susceptible citrus germplasm following infection with these two pathogens but not in trifoliate orange. These findings provide a comprehensive understanding of citrus root rot and support future research on the mechanisms of root rot resistance in citrus.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"19 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143477816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}