Horticultural Plant Journal最新文献

{"title":"Integrated metabolomic and transcriptomic profiling reveals the key role of UDP-glycosyltransferase 73D1 (UGT73D1) in rose under UV-B irradiation","authors":"Haoran Ren, Yuming Liu, Muhammad Owais Shahid, Xianhan Qiu, Wenjing Yang, Patrick Choisy, Tao Xu, Dan Wang, Huijun Yan, Hao Zhang, Nan Ma, Junping Gao, Xiaofeng Zhou, Weikun Jing","doi":"10.1016/j.hpj.2025.02.009","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.02.009","url":null,"abstract":"Ultraviolet B (UV–B) light affects the accumulation of secondary metabolites, especially pharmacologically important flavonoids, in many plants. However, whether UV-B irradiation influences the metabolite composition of rose (<ce:italic>Rosa</ce:italic> spp.) remains largely unknown. To explore the effects of UV-B on rose metabolites, we compared the physiological phenotypes of two rose cultivars, <ce:italic>Rosa hybrida</ce:italic> ‘Jardin de Granville’ (JDG) and <ce:italic>Rosa damascena</ce:italic> Mill. (DMS), under UV-B irradiation. JDG was more tolerant to UV-B exposure than DMS, with lower hydrogen peroxide content and electrolyte permeability and higher soluble protein content in leaves. To elucidate the mechanisms underlying this difference, we performed metabolome and transcriptome analyses of the two cultivars. Metabolome analysis showed that UV-B irradiation influenced the biosynthetic pathways for phenylpropanoid compounds, especially the flavonoid pathway, in both cultivars, resulting in significantly altered flavonol metabolite levels. RNA sequencing indicated that UV-B irradiation influenced plant hormone signaling and secondary metabolic pathways. Combined metabolomic and transcriptomic analysis highlighted key metabolites and associated genes in the flavonoid pathway that respond to UV-B irradiation. Simultaneously, weighted gene co-expression network analysis (WGCNA) revealed that <ce:italic>UDP glycosyltransferase 73D1</ce:italic> (<ce:italic>UGT73D1</ce:italic>) is crucial for flavonoid biosynthesis in rose under UV-B irradiation. Indeed, knocking down <ce:italic>UGT73D1</ce:italic> expression compromised the tolerance of rose to UV-B irradiation. Finally, dual-luciferase assays demonstrated that the transcription factor basic leucine-zipper 44 (bZIP44) enhances <ce:italic>UGT73D1</ce:italic> expression. Together, these results reveal that UV-B irradiation promotes flavonoid biosynthesis and metabolism in rose, providing an important theoretical foundation for cultivating rose germplasm with greater value for the pharmaceutical, food, and cosmetic industries.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"18 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143932457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"StCIPK2–StCBL11 complex negatively modulates drought tolerance in potato plant by regulating oxidative resistance and ABA biosynthesis","authors":"Rui Ma, Weigang Liu, Shigui Li, Xi Zhu, Jiangwei Yang, Ning Zhang, Huaijun Si","doi":"10.1016/j.hpj.2025.02.005","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.02.005","url":null,"abstract":"Calcineurin B-like (CBL) proteins and CBL-interacting protein kinases (CIPK) play essential roles in regulating plant responses to various stresses. However, the molecular and functional properties of CBL–CIPK in drought response have been rarely characterised in potato plants. In this study, we subjected the potato ‘Atlantic’ cultivar (wild type [WT], <ce:italic>ami-</ce:italic>StCIPK2, and overexpression [OE] lines) to stress through ABA treatment, osmotic stress, and drought stress. We characterised the potato CBL-interacting protein kinase StCIPK2. We measured the relative water content, superoxide dismutase and peroxidase activities, and H<ce:inf loc=\"post\">2</ce:inf>O<ce:inf loc=\"post\">2</ce:inf>, proline, malondialdehyde, and soluble sugar levels as these are indices of drought tolerance. We estimated the stomatal movement and root growth phenotype (length of plant roots). Finally, we performed a protein interaction assay. We found that <ce:italic>StCIPK2</ce:italic> was effectively induced by multiple stressors and is highly homologous to <ce:italic>AtCIPK1</ce:italic>. Accordingly, <ce:italic>StCIPK2</ce:italic> overexpression decreased drought tolerance and ABA sensitivity in potato, whereas <ce:italic>StCIPK2</ce:italic> knock-down increased these responses. Moreover, StCIPK2 interacted with StCBL11 on the plasma membrane. Additionally, the drought tolerance indices showed that drought resistance and ABA sensitivity did not differ between <ce:italic>StCBL11</ce:italic>-OE and WT plants, and <ce:italic>StCBL11</ce:italic> overexpression in <ce:italic>StCIPK2</ce:italic> knock-down plants negated their drought resistance and ABA sensitivity. Overall, this study shows that the StCBL11–StCIPK2 module negatively regulates drought stress tolerance in potatoes, and it could be used in novel ways to improve stress regulation in potato plant.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"10 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CmaGA2ox2 is associated with dwarf plant architecture controlled by the single dominant CmaDw-1 locus in winter squash","authors":"Chenggang Xiang, Ying Duan, Qin Shu, Kailiang Bo, Yiqun Weng, Xiaolei Sui, Changlin Wang","doi":"10.1016/j.hpj.2024.12.007","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.12.007","url":null,"abstract":"Dwarf or semi-dwarf plant architecture characterized by reduced vine or internode length is a desirable trait for winter squash (<ce:italic>Cucurbita maxima</ce:italic>) production, but the regulatory mechanisms and specific genes responsible for this trait remain unidentified. In this study, we conducted map-based QTL cloning and functional characterization of a major-effect QTL, underlying the dwarf phenotype (short vine length) in the inbred line Agol. A near-isogenic line, SV105 carrying the wildtype long-stem allele in Agol background was developed. Comparative histological observations in the two NILs indicated that the dwarf phenotype is due to reduced cell elongation, and thus internode length during stem elongation. Genetic analysis in NIL-derived segregating populations confirmed that a single dominant gene, <ce:italic>CmaDw-1, controlled</ce:italic> the dwarf phenotype. Bulked segregant analysis and fine genetic mapping identified <ce:italic>CmaCh03G013990</ce:italic> as the sole candidate gene for <ce:italic>CmaDw-</ce:italic>1 that encodes gibberellin 2-oxidase 2 (CmaGA2ox2), a key enzyme in gibberellin (GA) metabolism. Sequence analysis revealed multiple single nucleotide polymorphisms (SNPs) or Insert/Deletions (InDels) within the promoter and coding region of <ce:italic>CmaGA2ox2</ce:italic> that are associated with reduced stem elongation in Agol. Spatiotemporal expression analysis of <ce:italic>CmaGA2ox2</ce:italic> revealed its differential expression in the hypocotyls between NILs in the early development stage. Quantitation of endogenous GAs and exogenous phytohormone treatments in NILs and ectopic expression of <ce:italic>CmaDw-1</ce:italic> in <ce:italic>Arabidopsis</ce:italic> further support the critical role of <ce:italic>CmaGA2ox2</ce:italic> in stem elongation. This work provides the first molecular insights into the regulation of dwarf plant architecture in <ce:italic>C. maxima</ce:italic>.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"9 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influences of citrus apoplast pH on Xanthomonas citri subsp. citri invasion and canker formation","authors":"Rongchun Ye, Zhengmin Yang, Lian Liu, Jian Han, Limei Tan, Songliang Jiang, Chenxing Hao, Xianfeng Ma, Ziniu Deng","doi":"10.1016/j.hpj.2025.02.008","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.02.008","url":null,"abstract":"The pH plays a key role in the growth and colonization of plant pathogens as well as the onset and progression of the symptoms they cause within the host. Plants may quickly alter their apoplastic pH (pH<ce:inf loc=\"post\">apo</ce:inf>) to protect themselves against infection. However, pathogens can also alter the pH of their ambient environment to promote their own growth. Citrus canker is a serious plant disease caused by <ce:italic>Xanthomonas citri</ce:italic> subsp. <ce:italic>citri</ce:italic> (<ce:italic>Xcc</ce:italic>). This Gram-negative aerobic rod is usually cultured in Luria–Bertani (LB) medium at pH 7. However, little is known about the changes in pH both in this medium as <ce:italic>Xcc</ce:italic> grows and in the leaf apoplast in response to <ce:italic>Xcc</ce:italic> infection and colonization. Moreover, the differences in leaf apoplast pH between <ce:italic>Xcc</ce:italic>-resistant and <ce:italic>Xcc</ce:italic>-susceptible citrus genotypes are also unknown. Here, <ce:italic>Xcc</ce:italic> grew well in liquid LB medium at initial pH 6–8 and the pathogen altered the medium pH to 6.8 ± 0.4. <ce:italic>Xcc</ce:italic> growth declined at pH 5 and was zero at pH 3, 4, 9, and 10. In susceptible sweet orange infected with <ce:italic>Xcc</ce:italic> inoculum, canker symptoms were inhibited at pH 3, 4, and 10 but did not differ in the range of pH 5–9. As expected, canker symptoms were absent at all inoculum pH in the resistant Citron C-05. For both genotypes, <ce:italic>Xcc</ce:italic> only grew well in the leaves exposed to pH 5–8 inoculums. At four days post-inoculation (4 dpi), the foliar pH<ce:inf loc=\"post\">apo</ce:inf> of resistant Citron C-05 had rapidly declined from 5.6 to 4.4. At 2 dpi, the pH<ce:inf loc=\"post\">apo</ce:inf> of susceptible sweet orange had rapidly increased from 5.6 to 6.7, <ce:italic>Xcc</ce:italic> grew quickly, and canker symptoms appeared. Plasma membrane (PM) H<ce:sup loc=\"post\">+</ce:sup>-ATPase activation with fusicoccin (FC) acidified the apoplast and upregulated the pathogenesis-related genes (<ce:italic>PRs</ce:italic>) in the sweet orange leaves. Hence, <ce:italic>Xcc</ce:italic> colonization and canker development were inhibited. The results of this study revealed that apoplastic acidification is implicated in the resistance of Citron C-05 to <ce:italic>Xcc</ce:italic> infection and provided insight into the association between pH<ce:inf loc=\"post\">apo</ce:inf> regulation and resistance to bacterial pathogen invasion in plants.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"18 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microclimatic, physiological, and structural changes of tomato seedlings during wind-based overgrowth inhibition in vegetable nursery","authors":"Peiji Yang, Ying Liu, Jie Hao, Zhiguo Li, Fideline Tchuenbou-Magaia, Jiheng Ni","doi":"10.1016/j.hpj.2025.01.016","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.01.016","url":null,"abstract":"Wind disturbance has emerged as a potential eco-friendly method for seedling cultivation. In this study, an electromechanical device was designed and built to investigate the effects of airflow on the micro-environment and physiological activities of tomato seedlings in seedbeds by controlled experiments. The results indicated that airflow could enhance CO<ce:inf loc=\"post\">2</ce:inf> concentration near the seedling canopy, accelerate water evaporation from the seedling substrate, and reduce fluctuations in the temperature and humidity in microclimate. The photosynthetic rates of leaves at the 4th, 7th, and 10th positions in seedlings subjected to airflow increased by 25.04 %, 8.23 %, and 8.47 %, respectively, whereas the transpiration rates increased by 15.59 %, 22.28 %, and 13.26 %, respectively when compared to the control group. Additionally, the strong seedling index of seedlings treated with airflow and exogenous iron element increased by 26.02 % and 31.5 %, respectively. Compared to seedlings treated with exogenous iron element, the geometric mean diameter of the pith tissue cells in the stems of seedlings subjected to airflow disturbance was reduced by approximately 18.66 %, while the elastic modulus and bending strength of the stems increased by 10.01 % and 5.89 %, respectively. Similarly, the volume of root tissue cells decreased by 19.22 %, but the elastic modulus of the roots increased by 6.46 %. This study confirms that airflow significantly enhances seedling resilience to abiotic stress, yielding similar or better outcomes than exogenous iron application. It provides both theoretical and practical support for using airflow disturbance as a green technology for cultivating robust seedlings.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"17 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of m6A in plant development, stress response, and agricultural practices","authors":"Jin Qi, Shaoxia Li, Jun Su, Yushi Lu, Wenjin Yu, Changxia Li","doi":"10.1016/j.hpj.2025.02.006","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.02.006","url":null,"abstract":"N6-methyladenosine (m<ce:sup loc=\"post\">6</ce:sup>A) modification, the most abundant internal modification in messenger RNA (mRNA) and long non-coding RNA (lncRNA), has emerged as a critical epitranscriptomic regulatory mechanism in eukaryotes. While the importance of m<ce:sup loc=\"post\">6</ce:sup>A modification in various biological processes has been recognized, a comprehensive understanding of its diverse roles in plant biology and agricultural applications remains fragmented. This review analyzes recent advances in m<ce:sup loc=\"post\">6</ce:sup>A modification's biological functions in plants. m<ce:sup loc=\"post\">6</ce:sup>A modification plays crucial roles in multiple aspects of plant life, including seed germination, organ development, and reproductive structure formation. Furthermore, m<ce:sup loc=\"post\">6</ce:sup>A has been found to significantly influence plant responses to environmental stresses, including salt, drought, temperature, and heavy metal exposure. We also uncover m<ce:sup loc=\"post\">6</ce:sup>A involvement in important agricultural traits. This review provides insights into the mechanistic understanding of m<ce:sup loc=\"post\">6</ce:sup>A modification in plants and highlights its applications in agricultural improvement, offering a foundation for future research in crop enhancement and stress resistance.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"5 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatiotemporal and subgenome expression dynamics and regulatory divergence of the glucosinolate gene homologs in the allopolyploid Brassica juncea","authors":"Jinze Zhang, Shaomin Guo, Xu Yang, Lijing Xiao, Qingjing Ouyang, Hairun Jin, Xu Long, Zhongbin Yan, Entang Tian","doi":"10.1016/j.hpj.2025.02.007","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.02.007","url":null,"abstract":"Plant glucosinolates (GSLs) have attracted considerable attention due to their significant roles in plant defense mechanisms and cancer prevention. However, the gene expression characteristics and mechanisms underlying phenotypic variations in <ce:italic>Brassica juncea</ce:italic>, particularly in key vegetable and oilseed varieties, remain largely unexplored. This study examined the retention and the spatiotemporal expression and subgenome expression dynamics of duplicated genes predicted to regulate GSL metabolism in the allopolyploid <ce:italic>B. juncea</ce:italic>. Our findings reveal that homologs of <ce:italic>Arabidopsis</ce:italic> GSL genes (HAGG) are preferentially retained in the <ce:italic>B. juncea</ce:italic> genome compared to other genes. Most HAGG genes (76.5 %) were expressed across all tissues, while the remainders exhibited tissue-specific expression patterns. A majority of homoeologous pairs (54.09 %) demonstrated balanced expression levels, whereas the rest showed a pronounced A-genome dominance in leaves and stems. Notably, two <ce:italic>GRT2</ce:italic> homologs, <ce:italic>Bju.GTR2.A03</ce:italic> and <ce:italic>Bju.GTR2.B06</ce:italic>, which exhibit regulatory divergence in GSL accumulation, were identified. Co-dominant allele-specific markers developed for <ce:italic>Bju.GTR2.A03</ce:italic> and <ce:italic>Bju.GTR2.B06</ce:italic> colocalized with their respective quantitative trait loci (QTLs) and co-segregated with GSL content, corroborating the results of QTL mapping. Furthermore, GSL content was significantly correlated with resistance to <ce:italic>Sclerotinia sclerotiorum</ce:italic>. Predictions regarding the functions of HAGG genes further validated their functional divergence. This study provides a foundation for enhancing GSL content in whole plants or specific tissues, facilitating future applications in agriculture and cancer prevention in <ce:italic>B. juncea</ce:italic>.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"136 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome and metabolome analysis reveals regulatory network associated with light-independent anthocyanin biosynthesis in blueberry fruit","authors":"Xuyan Li, Shouwen Wang, Hongxue Li, Fangyun Qian, Haiyang Wang, Jingying Wang, Lulu Zhai, Baosheng Shi, Junwei Huo, Shaomin Bian","doi":"10.1016/j.hpj.2025.02.004","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.02.004","url":null,"abstract":"Anthocyanins are vital secondary metabolites contributing to fruit pigmentation and antioxidative properties. While light is a well-known regulator of anthocyanin biosynthesis, the molecular basis of light-independent anthocyanin accumulation remains underexplored. In this study, integrated analysis of metabolome and transcriptome showed that the anthocyanin content in blueberry (<ce:italic>Vaccinium corymbosum</ce:italic> ‘Bluetta’) fruit was slightly decreased by light-impermeable bagging treatment, while anthocyanin biosynthetic genes were transcriptionally inhibited to different levels, suggesting a slight influence of the bagging treatment on anthocyanin accumulation. Further observation showed that fruit bagging did not alter ethylene production but decreased ABA content. Noticeably, two <ce:italic>VcMYBA/MYB1</ce:italic>s were not transcriptionally altered by the light-impermeable bagging treatment. Consistently, histochemical GUS analysis and pharmacological manipulation suggested light-independent and ethylene-inducible expression of <ce:italic>VcMYBA/MYB1</ce:italic>. Moreover, WGCNA analysis revealed 3759 genes positively associated with <ce:italic>MYBA/MYB1</ce:italic> such as ethylene-associated genes, etc. Additionally, <ce:italic>VcbZIP55</ce:italic>s and <ce:italic>VcCOP1</ce:italic>s were activated and inactivated by the bagging treatment, respectively. These findings provided a framework of light-independent anthocyanin biosynthesis in blueberry fruit.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"24 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein post-translational modification in plants: Regulation and beyond","authors":"Zhenxiang Li, Liqian Qin, Dongjiao Wang, Tianzhen Cui, Yanlan Liang, Shoujian Zang, Tingting Sun, Wanying Zhao, Qibin Wu, Youxiong Que","doi":"10.1016/j.hpj.2025.02.003","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.02.003","url":null,"abstract":"Post-translational modification (PTM) of proteins is a crucial regulatory mechanism in plant cells, enabling rapid and purposeful regulation of their functions. Modified proteins play various roles in signaling pathways, including plant growth and development, plant metabolism, and the response to adversity stress. In recent years, there has been an increase in the number of studies focusing on plant PTM maps and functional analysis. Here we aim to review the PTM types in plants, especially in horticultural plants and tropical crops, the interactions between and among PTMs, and more importantly, the underlying pathways and functions. Additionally, the potential application of PTMs in breeding is briefly discussed. This paper focuses on plant PTMs and provides a foundation for further investigation into the functions and regulatory mechanisms of PTMs in plant proteins as well as for crop improvement.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"23 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide analyses of RWP–RK reveal a potential role for a key gene, VvNLP1.1, in the grapevine response to nitrate","authors":"Xin Yang, Jingwen Li, Guipeng Liu, Lu Bian, Mingxin Feng, Yujia Liu, Kai Li, Jiayin Shang, Yulin Fang, Tengfei Xu, Jiangfei Meng","doi":"10.1016/j.hpj.2025.02.002","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.02.002","url":null,"abstract":"Nitrate is the primary nitrogen source for plants and is a signaling molecule regulating various plant developmental processes. Despite its significance, limited information is available on nitrate signaling in <ce:italic>Vitis vinifera</ce:italic>. We identified nine <ce:italic>VvRWP–RK</ce:italic> genes distributed across eight chromosomes using genome–wide identification and evolutionary analyses. Among these, <ce:italic>VvNLP1–4</ce:italic> and <ce:italic>VvRKD1–5</ce:italic> are associated with nitrate signaling and reproductive growth, respectively. To investigate their potential functions, structures, <ce:italic>cis</ce:italic>–acting promoter elements, functional structural domains, phylogenetic trees, spatiotemporal expression levels in different tissues at different developmental stages, potential protein–protein interaction networks, synteny (gene content), collinearity (gene order), and three–dimensional protein structure prediction were explored. We found that long–term nitrate application dramatically promoted grapevine plantlet development, including primary root length and leaf growth, and <ce:italic>VvNLP1.1</ce:italic>, <ce:italic>VvNLP1.2</ce:italic>, and <ce:italic>VvNLP2</ce:italic> were highly expressed in ‘Thompson Seedless' root tissues under nitrate–enriched conditions. To clarify the critical role of nitrate in grapevine growth, we observed that nuclear localization of VvNLP1.1 increased significantly following nitrate treatment. VvNLP1.1 was found to bind to the promoter of the primary nitrate response gene <ce:italic>VvNRT1.</ce:italic>1, driving its transcriptional activity. These findings indicate that <ce:italic>VvNLP1.1</ce:italic> is a core transcription factor of the nitrate signaling pathway in grapevine. Nitrate molecular docking analysis revealed that VvNLP1.1 directly binds to nitrate ions, indicating its potential role as a nitrate sensor capable of directly perceiving nitrate concentration. We also discovered that short–term nitrate starvation impacts <ce:italic>VvNLP1.1</ce:italic> promoter activity, linked to the abscisic acid–binding element (ABRE) motif in its promoter region. Our results thus provide new insights into the molecular mechanisms underlying various physiological processes in grapevine, particularly the nitrate signaling pathway, and provide a theoretical basis for improving nitrogen use efficiency (NUE) in grapevine.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"75 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}