在乌龙茶加工过程中,UV-B和机械损伤通过CsHY5-CsMYC2协同诱导α-法脂烯生物合成

IF 6.2 1区 农林科学 Q1 HORTICULTURE
Jingjie Cao, Dandan Li, Xiaohui Wang, Xin He, Ying Sun, Xin Cheng, Xiaochun Wan, Linlin Liu
{"title":"在乌龙茶加工过程中,UV-B和机械损伤通过CsHY5-CsMYC2协同诱导α-法脂烯生物合成","authors":"Jingjie Cao, Dandan Li, Xiaohui Wang, Xin He, Ying Sun, Xin Cheng, Xiaochun Wan, Linlin Liu","doi":"10.1016/j.hpj.2025.01.015","DOIUrl":null,"url":null,"abstract":"UV-B application enhances the aroma quality of oolong tea; however, the underlying regulatory mechanism remains unclear. This study investigates the regulatory role of UV-B in the biosynthesis of <ce:italic>α</ce:italic>-farnesene, an important floral and fruity characteristic aroma. UV-B treatment significantly improved the aroma quality of ‘Foshou’ and ‘Yuquan’ oolong teas, increasing <ce:italic>α</ce:italic>-farnesene levels by 1.8- and 1.4-fold, respectively. The <ce:italic>α-farnesene synthase</ce:italic> (<ce:italic>CsAFS</ce:italic>), <ce:italic>ELONGATED HYPOCOTYL 5</ce:italic> (<ce:italic>CsHY5</ce:italic>), and <ce:italic>myelocytomatosis protein 2</ce:italic> (<ce:italic>CsMYC2</ce:italic>) exhibited a highly correlated expression pattern closely associated with <ce:italic>α</ce:italic>-farnesene accumulation. Single-factor treatment revealed that <ce:italic>CsAFS</ce:italic> expression was induced by both UV-B and mechanical wounding, with <ce:italic>CsHY5</ce:italic> predominantly responding to UV-B radiation, while <ce:italic>CsMYC2</ce:italic> primarily responded to tumbling-induced mechanical wounding signal. Transient suppression of CsHY5 in tea leaves reduced the expression of both <ce:italic>CsAFS</ce:italic> and <ce:italic>CsMYC2</ce:italic> whereas <ce:italic>CsMYC2</ce:italic> suppression decreased <ce:italic>CsAFS</ce:italic> expression. G-box motifs were identified in promoters of <ce:italic>CsMYC2</ce:italic> and <ce:italic>CsAFS</ce:italic>, and the dual-luciferase reporter assay (LUC) and electrophoretic mobility shift assays (EMSA) demonstrated direct binding functions of CsHY5 to <ce:italic>CsAFS</ce:italic> and <ce:italic>CsMYC2</ce:italic> promoters, as well as CsMYC2 to the <ce:italic>CsAFS</ce:italic> promoter<ce:italic>.</ce:italic> Based on sensory evaluation, odourant quantification, gene expression, and molecular functional analysis, we propose that UV-B radiation and tumbling-induced wounding signals synergistically regulate <ce:italic>α</ce:italic>-farnesene biosynthesis through a coordinated interaction of CsHY5 and CsMYC2 during oolong tea processing. These findings improve our understanding of flavour formation during oolong tea production and also provide novel insights into artificial light application in tea manufacturing.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"42 1","pages":""},"PeriodicalIF":6.2000,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"UV-B and mechanical wounding synergistically induce α-farnesene biosynthesis via CsHY5-CsMYC2 cooperation during oolong tea processing\",\"authors\":\"Jingjie Cao, Dandan Li, Xiaohui Wang, Xin He, Ying Sun, Xin Cheng, Xiaochun Wan, Linlin Liu\",\"doi\":\"10.1016/j.hpj.2025.01.015\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"UV-B application enhances the aroma quality of oolong tea; however, the underlying regulatory mechanism remains unclear. This study investigates the regulatory role of UV-B in the biosynthesis of <ce:italic>α</ce:italic>-farnesene, an important floral and fruity characteristic aroma. UV-B treatment significantly improved the aroma quality of ‘Foshou’ and ‘Yuquan’ oolong teas, increasing <ce:italic>α</ce:italic>-farnesene levels by 1.8- and 1.4-fold, respectively. The <ce:italic>α-farnesene synthase</ce:italic> (<ce:italic>CsAFS</ce:italic>), <ce:italic>ELONGATED HYPOCOTYL 5</ce:italic> (<ce:italic>CsHY5</ce:italic>), and <ce:italic>myelocytomatosis protein 2</ce:italic> (<ce:italic>CsMYC2</ce:italic>) exhibited a highly correlated expression pattern closely associated with <ce:italic>α</ce:italic>-farnesene accumulation. Single-factor treatment revealed that <ce:italic>CsAFS</ce:italic> expression was induced by both UV-B and mechanical wounding, with <ce:italic>CsHY5</ce:italic> predominantly responding to UV-B radiation, while <ce:italic>CsMYC2</ce:italic> primarily responded to tumbling-induced mechanical wounding signal. Transient suppression of CsHY5 in tea leaves reduced the expression of both <ce:italic>CsAFS</ce:italic> and <ce:italic>CsMYC2</ce:italic> whereas <ce:italic>CsMYC2</ce:italic> suppression decreased <ce:italic>CsAFS</ce:italic> expression. G-box motifs were identified in promoters of <ce:italic>CsMYC2</ce:italic> and <ce:italic>CsAFS</ce:italic>, and the dual-luciferase reporter assay (LUC) and electrophoretic mobility shift assays (EMSA) demonstrated direct binding functions of CsHY5 to <ce:italic>CsAFS</ce:italic> and <ce:italic>CsMYC2</ce:italic> promoters, as well as CsMYC2 to the <ce:italic>CsAFS</ce:italic> promoter<ce:italic>.</ce:italic> Based on sensory evaluation, odourant quantification, gene expression, and molecular functional analysis, we propose that UV-B radiation and tumbling-induced wounding signals synergistically regulate <ce:italic>α</ce:italic>-farnesene biosynthesis through a coordinated interaction of CsHY5 and CsMYC2 during oolong tea processing. These findings improve our understanding of flavour formation during oolong tea production and also provide novel insights into artificial light application in tea manufacturing.\",\"PeriodicalId\":13178,\"journal\":{\"name\":\"Horticultural Plant Journal\",\"volume\":\"42 1\",\"pages\":\"\"},\"PeriodicalIF\":6.2000,\"publicationDate\":\"2025-03-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Horticultural Plant Journal\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1016/j.hpj.2025.01.015\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"HORTICULTURE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Horticultural Plant Journal","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.hpj.2025.01.015","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"HORTICULTURE","Score":null,"Total":0}
引用次数: 0

摘要

UV-B的应用提高了乌龙茶的香气品质;然而,潜在的监管机制仍不清楚。本研究探讨了UV-B在α-法尼烯生物合成中的调节作用,α-法尼烯是一种重要的花果特征香气。UV-B处理显著改善了“佛寿”和“玉泉”乌龙茶的香气品质,α-法尼烯含量分别提高了1.8倍和1.4倍。α-法尼烯合成酶(CsAFS)、细长下胚轴5 (CsHY5)和髓细胞瘤病蛋白2 (CsMYC2)的表达模式与α-法尼烯积累密切相关。单因素处理表明,CsAFS表达受UV-B和机械损伤的双重诱导,CsHY5主要响应UV-B辐射,而CsMYC2主要响应翻转诱导的机械损伤信号。茶叶中CsHY5的瞬时抑制降低了CsAFS和CsMYC2的表达,而CsMYC2的抑制则降低了CsAFS的表达。在CsMYC2和CsAFS的启动子中发现了G-box基序,双荧光素酶报告试验(LUC)和电泳迁移率转移试验(EMSA)表明CsHY5与CsAFS和CsMYC2启动子以及CsMYC2与CsAFS启动子具有直接结合功能。基于感官评价、气味定量、基因表达和分子功能分析,我们提出在乌龙茶加工过程中,UV-B辐射和震荡损伤信号通过CsHY5和CsMYC2的协同作用协同调节α-法脂烯的生物合成。这些发现提高了我们对乌龙茶生产过程中风味形成的理解,也为人工光在茶叶生产中的应用提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
UV-B and mechanical wounding synergistically induce α-farnesene biosynthesis via CsHY5-CsMYC2 cooperation during oolong tea processing
UV-B application enhances the aroma quality of oolong tea; however, the underlying regulatory mechanism remains unclear. This study investigates the regulatory role of UV-B in the biosynthesis of α-farnesene, an important floral and fruity characteristic aroma. UV-B treatment significantly improved the aroma quality of ‘Foshou’ and ‘Yuquan’ oolong teas, increasing α-farnesene levels by 1.8- and 1.4-fold, respectively. The α-farnesene synthase (CsAFS), ELONGATED HYPOCOTYL 5 (CsHY5), and myelocytomatosis protein 2 (CsMYC2) exhibited a highly correlated expression pattern closely associated with α-farnesene accumulation. Single-factor treatment revealed that CsAFS expression was induced by both UV-B and mechanical wounding, with CsHY5 predominantly responding to UV-B radiation, while CsMYC2 primarily responded to tumbling-induced mechanical wounding signal. Transient suppression of CsHY5 in tea leaves reduced the expression of both CsAFS and CsMYC2 whereas CsMYC2 suppression decreased CsAFS expression. G-box motifs were identified in promoters of CsMYC2 and CsAFS, and the dual-luciferase reporter assay (LUC) and electrophoretic mobility shift assays (EMSA) demonstrated direct binding functions of CsHY5 to CsAFS and CsMYC2 promoters, as well as CsMYC2 to the CsAFS promoter. Based on sensory evaluation, odourant quantification, gene expression, and molecular functional analysis, we propose that UV-B radiation and tumbling-induced wounding signals synergistically regulate α-farnesene biosynthesis through a coordinated interaction of CsHY5 and CsMYC2 during oolong tea processing. These findings improve our understanding of flavour formation during oolong tea production and also provide novel insights into artificial light application in tea manufacturing.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Horticultural Plant Journal
Horticultural Plant Journal Environmental Science-Ecology
CiteScore
9.60
自引率
14.00%
发文量
293
审稿时长
33 weeks
期刊介绍: Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信