Histochemistry and Cell Biology最新文献_第4页

In focus in HCB. 重点关注 HCB。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-09-01 DOI: 10.1007/s00418-024-02315-8

Douglas J Taatjes, Jürgen Roth

引用次数: 0

Effect of elevated temperature and hydrocortisone addition on the proliferation of fibroblasts. 高温和氢化可的松对成纤维细胞增殖的影响

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-09-01 Epub Date: 2024-05-27 DOI: 10.1007/s00418-024-02295-9

Zuzana Pavlikova, Oldrich Zahradnicek, Anna Jelinek Michaelidesova, Jaromir Sramek, Marie Davidkova, Maria Hovorakova

{"title":"Effect of elevated temperature and hydrocortisone addition on the proliferation of fibroblasts.","authors":"Zuzana Pavlikova, Oldrich Zahradnicek, Anna Jelinek Michaelidesova, Jaromir Sramek, Marie Davidkova, Maria Hovorakova","doi":"10.1007/s00418-024-02295-9","DOIUrl":"10.1007/s00418-024-02295-9","url":null,"abstract":"Hyperthermia along with hydrocortisone (HC) are proven teratogens that can negatively influence embryo development during early pregnancy. Proliferation of cells is one of the main developmental processes during the early embryogenesis. This study was focused on testing the effect of elevated temperature and HC addition on proliferation of cells in in vitro cultures. The V79-4 cell line was treated with HC and cultured in vitro at 37 °C or 39 °C, respectively. To reveal the effect of both factors, the proliferation of cells cultured under different conditions was evaluated using various approaches (colony formation assay, generation of growth curves, computation of doubling times, and mitotic index estimation). Our results indicate that a short-term exposure to elevated temperature slightly stimulates and a long-term exposure suppresses cell proliferation. However, HC (0.1 mg/ml) acts as a stimulator of cell proliferation. Interestingly, the interaction of HC and long-term elevated temperature (39 °C) exposure results in at least partial compensation of the negative impact of elevated temperature by HC addition and in higher proliferation if compared with cells cultured at 39 °C without addition of HC.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":" ","pages":"231-244"},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11322275/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential expression of small bowel TGFβ1 and TGFβ3 characterizes intestinal strictures in patients with fibrostenotic Crohn's disease. 小肠 TGFβ1 和 TGFβ3 的差异表达是纤维性克罗恩病患者肠道狭窄的特征。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-09-01 Epub Date: 2024-05-05 DOI: 10.1007/s00418-024-02290-0

Steven Levitte, Ibaad Khan, Violet Iyahen, James Ziai, John Gubatan, Rebecca Sheng, Sara B Glickstein, Tianhe Sun, K T Park, Jacqueline McBride, Mary Keir

{"title":"Differential expression of small bowel TGFβ1 and TGFβ3 characterizes intestinal strictures in patients with fibrostenotic Crohn's disease.","authors":"Steven Levitte, Ibaad Khan, Violet Iyahen, James Ziai, John Gubatan, Rebecca Sheng, Sara B Glickstein, Tianhe Sun, K T Park, Jacqueline McBride, Mary Keir","doi":"10.1007/s00418-024-02290-0","DOIUrl":"10.1007/s00418-024-02290-0","url":null,"abstract":"Small bowel strictures remain a debilitating consequence of Crohn's disease and contribute to poor outcomes for patients. Recently, TGFβ has been identified as an important driver of intestinal fibrosis. We studied the localization of TGFβ isoforms in ileal strictures of patients with Crohn's disease using in situ hybridization to understand TGFβ's role in stricture formation. The mucosa of strictures was characterized by higher TGFβ1 while the stricture submucosa showed higher TGFβ3 compared to normal ileum from patients without Crohn's disease (p = 0.02 and p = 0.044, respectively). We correlated these findings with single-cell transcriptomics which demonstrated that TGFβ3 transcripts overall are very rare, which may partially explain why its role in intestinal fibrosis has remained unclear to date. There were no significant differences in fibroblast or B cell TGFβ1 and/or TGFβ3 expression in inflamed vs. noninflamed ileum. We discuss the implications of these findings for therapeutic development strategies to treat patients with fibrostenotic Crohn's disease.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":" ","pages":"225-230"},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The emerging role of the semaphorin family in cartilage and osteoarthritis. semaphorin家族在软骨和骨关节炎中的新作用。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-09-01 Epub Date: 2024-06-07 DOI: 10.1007/s00418-024-02303-y

Wenjing Peng, Qian Chen, Fengjuan Zheng, Li Xu, Xinyi Fang, Zuping Wu

引用次数: 0

CCAT1 lncRNA is chromatin-retained and post-transcriptionally spliced. CCAT1 lncRNA具有染色质保留和转录后剪接功能。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-07-01 Epub Date: 2024-05-19 DOI: 10.1007/s00418-024-02294-w

Chaya Bohrer, Eli Varon, Eldar Peretz, Gita Reinitz, Noa Kinor, David Halle, Aviram Nissan, Yaron Shav-Tal

{"title":"CCAT1 lncRNA is chromatin-retained and post-transcriptionally spliced.","authors":"Chaya Bohrer, Eli Varon, Eldar Peretz, Gita Reinitz, Noa Kinor, David Halle, Aviram Nissan, Yaron Shav-Tal","doi":"10.1007/s00418-024-02294-w","DOIUrl":"10.1007/s00418-024-02294-w","url":null,"abstract":"Super-enhancers are unique gene expression regulators widely involved in cancer development. Spread over large DNA segments, they tend to be found next to oncogenes. The super-enhancer c-MYC locus forms long-range chromatin looping with nearby genes, which brings the enhancer and the genes into proximity, to promote gene activation. The colon cancer-associated transcript 1 (CCAT1) gene, which is part of the MYC locus, transcribes a lncRNA that is overexpressed in colon cancer cells through activation by MYC. Comparing different types of cancer cell lines using RNA fluorescence in situ hybridization (RNA FISH), we detected very prominent CCAT1 expression in HeLa cells, observed as several large CCAT1 nuclear foci. We found that dozens of CCAT1 transcripts accumulate on the gene locus, in addition to active transcription occurring from the gene. The accumulating transcripts are released from the chromatin during cell division. Examination of CCAT1 lncRNA expression patterns on the single-RNA level showed that unspliced CCAT1 transcripts are released from the gene into the nucleoplasm. Most of these unspliced transcripts were observed in proximity to the active gene but were not associated with nuclear speckles in which unspliced RNAs usually accumulate. At larger distances from the gene, the CCAT1 transcripts appeared spliced, implying that most CCAT1 transcripts undergo post-transcriptional splicing in the zone of the active gene. Finally, we show that unspliced CCAT1 transcripts can be detected in the cytoplasm during splicing inhibition, which suggests that there are several CCAT1 variants, spliced and unspliced, that the cell can recognize as suitable for export.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":" ","pages":"91-107"},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11227459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141064993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustained inactivation of the Polycomb PRC1 complex induces DNA repair defects and genomic instability in epigenetic tumors. 多聚胞 PRC1 复合物的持续失活会诱发表观遗传肿瘤中的 DNA 修复缺陷和基因组不稳定性。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-07-01 Epub Date: 2024-06-18 DOI: 10.1007/s00418-024-02302-z

Chetan C Rawal, Vincent Loubiere, Nadejda L Butova, Juliette Gracia, Victoria Parreno, Chiara Merigliano, Anne-Marie Martinez, Giacomo Cavalli, Irene Chiolo

{"title":"Sustained inactivation of the Polycomb PRC1 complex induces DNA repair defects and genomic instability in epigenetic tumors.","authors":"Chetan C Rawal, Vincent Loubiere, Nadejda L Butova, Juliette Gracia, Victoria Parreno, Chiara Merigliano, Anne-Marie Martinez, Giacomo Cavalli, Irene Chiolo","doi":"10.1007/s00418-024-02302-z","DOIUrl":"10.1007/s00418-024-02302-z","url":null,"abstract":"Cancer initiation and progression are typically associated with the accumulation of driver mutations and genomic instability. However, recent studies demonstrated that cancer can also be driven purely by epigenetic alterations, without driver mutations. Specifically, a 24-h transient downregulation of polyhomeotic (ph-KD), a core component of the Polycomb complex PRC1, is sufficient to induce epigenetically initiated cancers (EICs) in Drosophila, which are proficient in DNA repair and characterized by a stable genome. Whether genomic instability eventually occurs when PRC1 downregulation is performed for extended periods of time remains unclear. Here, we show that prolonged depletion of PH, which mimics cancer initiating events, results in broad dysregulation of DNA replication and repair genes, along with the accumulation of DNA breaks, defective repair, and widespread genomic instability in the cancer tissue. A broad misregulation of H2AK118 ubiquitylation and to a lesser extent of H3K27 trimethylation also occurs and might contribute to these phenotypes. Together, this study supports a model where DNA repair and replication defects accumulate during the tumorigenic transformation epigenetically induced by PRC1 loss, resulting in genomic instability and cancer progression.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":" ","pages":"133-147"},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11227471/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141418698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visualizing histone H4K20me1 in knock-in mice expressing the mCherry-tagged modification-specific intracellular antibody. 在表达 mCherry 标记修饰特异性细胞内抗体的基因敲入小鼠中观察组蛋白 H4K20me1。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-07-01 Epub Date: 2024-05-19 DOI: 10.1007/s00418-024-02296-8

Yuko Sato, Maoko Takenoshita, Miku Ueoka, Jun Ueda, Kazuo Yamagata, Hiroshi Kimura

{"title":"Visualizing histone H4K20me1 in knock-in mice expressing the mCherry-tagged modification-specific intracellular antibody.","authors":"Yuko Sato, Maoko Takenoshita, Miku Ueoka, Jun Ueda, Kazuo Yamagata, Hiroshi Kimura","doi":"10.1007/s00418-024-02296-8","DOIUrl":"10.1007/s00418-024-02296-8","url":null,"abstract":"During development and differentiation, histone modifications dynamically change locally and globally, associated with transcriptional regulation, DNA replication and repair, and chromosome condensation. The level of histone H4 Lys20 monomethylation (H4K20me1) increases during the G2 to M phases of the cell cycle and is enriched in facultative heterochromatin, such as inactive X chromosomes in cycling cells. To track the dynamic changes of H4K20me1 in living cells, we have developed a genetically encoded modification-specific intracellular antibody (mintbody) probe that specifically binds to the modification. Here, we report the generation of knock-in mice in which the coding sequence of the mCherry-tagged version of the H4K20me1-mintbody is inserted into the Rosa26 locus. The knock-in mice, which ubiquitously expressed the H4K20me1-mintbody, developed normally and were fertile, indicating that the expression of the probe does not disturb the cell growth, development, or differentiation. Various tissues isolated from the knock-in mice exhibited nuclear fluorescence without the need for fixation. The H4K20me1-mintbody was enriched in inactive X chromosomes in developing embryos and in XY bodies during spermatogenesis. The knock-in mice will be useful for the histochemical analysis of H4K20me1 in any cell types.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":" ","pages":"41-52"},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11227479/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140957074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seeing genomes. 看到基因组

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-07-01 DOI: 10.1007/s00418-024-02301-0

Tom Misteli

引用次数: 0

Crossing boundaries of light microscopy resolution discerns novel assemblies in the nucleolus. 跨越光镜分辨率的界限，发现核仁中的新型组合。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-07-01 Epub Date: 2024-05-17 DOI: 10.1007/s00418-024-02297-7

Carl C Correll, Udo Rudloff, Jeremy D Schmit, David A Ball, Tatiana S Karpova, Eric Balzer, Miroslav Dundr

{"title":"Crossing boundaries of light microscopy resolution discerns novel assemblies in the nucleolus.","authors":"Carl C Correll, Udo Rudloff, Jeremy D Schmit, David A Ball, Tatiana S Karpova, Eric Balzer, Miroslav Dundr","doi":"10.1007/s00418-024-02297-7","DOIUrl":"10.1007/s00418-024-02297-7","url":null,"abstract":"The nucleolus is the largest membraneless organelle and nuclear body in mammalian cells. It is primarily involved in the biogenesis of ribosomes, essential macromolecular machines responsible for synthesizing all proteins required by the cell. The assembly of ribosomes is evolutionarily conserved and accounts for the most energy-consuming cellular process needed for cell growth, proliferation, and homeostasis. Despite the significance of this process, the substructural mechanistic principles of the nucleolar function in preribosome biogenesis have only recently begun to emerge. Here, we provide a new perspective using advanced super-resolution microscopy and single-molecule MINFLUX nanoscopy on the mechanistic principles governing ribosomal RNA-seeded nucleolar formation and the resulting tripartite suborganization of the nucleolus driven, in part, by liquid-liquid phase separation. With recent advances in the cryogenic electron microscopy (cryoEM) structural analysis of ribosome biogenesis intermediates, we highlight the current understanding of the step-wise assembly of preribosomal subunits in the nucleolus. Finally, we address how novel anticancer drug candidates target early steps in ribosome biogenesis to exploit these essential dependencies for growth arrest and tumor control.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":" ","pages":"161-183"},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11330670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140957002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic chromosome association with nuclear organelles in living cells. 活细胞中染色体与核细胞器的动态结合。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-07-01 Epub Date: 2024-05-30 DOI: 10.1007/s00418-024-02288-8

Lam Minh Uyen Phan, Wei-Hong Yeo, Hao F Zhang, Sui Huang

{"title":"Dynamic chromosome association with nuclear organelles in living cells.","authors":"Lam Minh Uyen Phan, Wei-Hong Yeo, Hao F Zhang, Sui Huang","doi":"10.1007/s00418-024-02288-8","DOIUrl":"10.1007/s00418-024-02288-8","url":null,"abstract":"The development of progressively sophisticated tools complemented by the integration of live cell imaging enhances our understanding of the four-dimensional (4D) nucleome, revealing elaborate molecular interactions and chromatin states. Yet, the dynamics of chromosomes in relation to nuclear organelles or to each other across cell cycle in living cells are underexplored. We have developed photoconvertible GFP H3-Dendra2 stably expressing in PC3M cells. The nuclear lamina and perinucleolar associated heterochromatin or diffuse chromosome regions were photoconverted through a single-point activation using a confocal microscope. The results demonstrated a dynamic nature for both types of chromosomes in the same cell cycle and across mitosis. While some chromosome domains were heritably associated with either nuclear lamina or nucleoli, others changed alliance to different nuclear organelles postmitotically. In addition, co-photoconverted chromosome domains often do not stay together within the same cell cycle and across mitosis, suggesting a transient nature of chromosome neighborhoods. Long-range spreading and movement of chromosomes were also observed. Interestingly, when cells were treated with a low concentration of actinomycin D that inhibits Pol I transcription through intercalating GC-rich DNA, chromosome movement was significantly blocked. Treatment with another Pol I inhibitor, metarrestin, which does not impact DNA, had little effect on the movement, suggesting that the DNA structure itself plays a role in chromosome dynamics. Furthermore, inhibition of Pol II transcription with α-amanitin also reduced the chromosome movement, demonstrating that Pol II, but not Pol I transcription, is important for chromosome dynamics in the nucleus.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":" ","pages":"149-159"},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141175210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0