Histochemistry and Cell Biology最新文献_第5页

Allele-level visualization of transcription and chromatin by high-throughput imaging. 通过高通量成像技术实现转录和染色质的等位基因级可视化。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-07-01 Epub Date: 2024-05-09 DOI: 10.1007/s00418-024-02289-7

Faisal Almansour, Adib Keikhosravi, Gianluca Pegoraro, Tom Misteli

{"title":"Allele-level visualization of transcription and chromatin by high-throughput imaging.","authors":"Faisal Almansour, Adib Keikhosravi, Gianluca Pegoraro, Tom Misteli","doi":"10.1007/s00418-024-02289-7","DOIUrl":"10.1007/s00418-024-02289-7","url":null,"abstract":"The spatial arrangement of the genome within the nucleus is a pivotal aspect of cellular organization and function with implications for gene expression and regulation. While all genome organization features, such as loops, domains, and radial positioning, are nonrandom, they are characterized by a high degree of single-cell variability. Imaging approaches are ideally suited to visualize, measure, and study single-cell heterogeneity in genome organization. Here, we describe two methods for the detection of DNA and RNA of individual gene alleles by fluorescence in situ hybridization (FISH) in a high-throughput format. We have optimized combined DNA/RNA FISH approaches either using simultaneous or sequential detection of DNA and nascent RNA. These optimized DNA and RNA FISH protocols were implemented in a 384-well plate format alongside automated image and data analysis and enable accurate detection of individual gene alleles and their gene expression status across a large cell population. We successfully visualized MYC and EGFR DNA and nascent RNA with allele-level resolution in multiple cell types, and we determined the radial position of active and inactive MYC and EGFR alleles. These optimized DNA/RNA detection approaches are versatile and sensitive tools for mapping of chromatin features and gene activity at the single-allele level and at high throughput.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":" ","pages":"65-77"},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11227451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Behaviors of nucleosomes with mutant histone H4s in euchromatic domains of living human cells. 活体人体细胞中带有突变组蛋白 H4 的核小体的行为。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-07-01 Epub Date: 2024-05-14 DOI: 10.1007/s00418-024-02293-x

Adilgazy Semeigazin, Shiori Iida, Katsuhiko Minami, Sachiko Tamura, Satoru Ide, Koichi Higashi, Atsushi Toyoda, Ken Kurokawa, Kazuhiro Maeshima

{"title":"Behaviors of nucleosomes with mutant histone H4s in euchromatic domains of living human cells.","authors":"Adilgazy Semeigazin, Shiori Iida, Katsuhiko Minami, Sachiko Tamura, Satoru Ide, Koichi Higashi, Atsushi Toyoda, Ken Kurokawa, Kazuhiro Maeshima","doi":"10.1007/s00418-024-02293-x","DOIUrl":"10.1007/s00418-024-02293-x","url":null,"abstract":"Since Robert Feulgen first stained DNA in the cell, visualizing genome chromatin has been a central issue in cell biology to uncover how chromatin is organized and behaves in the cell. To approach this issue, we have developed single-molecule imaging of nucleosomes, a basic unit of chromatin, to unveil local nucleosome behavior in living cells. In this study, we investigated behaviors of nucleosomes with various histone H4 mutants in living HeLa cells to address the role of H4 tail acetylation, including H4K16Ac and others, which are generally associated with more transcriptionally active chromatin regions. We ectopically expressed wild-type (wt) or mutated H4s (H4K16 point; H4K5,8,12,16 quadruple; and H4 tail deletion) fused with HaloTag in HeLa cells. Cells that expressed wtH4-Halo, H4K16-Halo mutants, and multiple H4-Halo mutants had euchromatin-concentrated distribution. Consistently, the genomic regions of the wtH4-Halo nucleosomes corresponded to Hi-C contact domains (or topologically associating domains, TADs) with active chromatin marks (A-compartment). Utilizing single-nucleosome imaging, we found that none of the H4 deacetylation or acetylation mimicked H4 mutants altered the overall local nucleosome motion. This finding suggests that H4 mutant nucleosomes embedded in the condensed euchromatic domains with excess endogenous H4 nucleosomes cannot cause an observable change in the local motion. Interestingly, H4 with four lysine-to-arginine mutations displayed a substantial freely diffusing fraction in the nucleoplasm, whereas H4 with a truncated N-terminal tail was incorporated in heterochromatic regions as well as euchromatin. Our study indicates the power of single-nucleosome imaging to understand individual histone/nucleosome behavior reflecting chromatin environments in living cells.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":" ","pages":"23-40"},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140921350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From feulgen to modern methods: marking a century of DNA imaging advances. 从费尔根到现代方法：DNA 成像发展的一个世纪。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-07-01 Epub Date: 2024-05-16 DOI: 10.1007/s00418-024-02291-z

Melike Lakadamyali

引用次数: 0

DNA choreography: correlating mobility and organization of DNA across different resolutions from loops to chromosomes. DNA 编排：从环到染色体，DNA 在不同分辨率下的移动性和组织相关性。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-07-01 Epub Date: 2024-05-17 DOI: 10.1007/s00418-024-02285-x

Maruthi K Pabba, Janis Meyer, Kerem Celikay, Lothar Schermelleh, Karl Rohr, M Cristina Cardoso

{"title":"DNA choreography: correlating mobility and organization of DNA across different resolutions from loops to chromosomes.","authors":"Maruthi K Pabba, Janis Meyer, Kerem Celikay, Lothar Schermelleh, Karl Rohr, M Cristina Cardoso","doi":"10.1007/s00418-024-02285-x","DOIUrl":"10.1007/s00418-024-02285-x","url":null,"abstract":"The dynamics of DNA in the cell nucleus plays a role in cellular processes and fates but the interplay of DNA mobility with the hierarchical levels of DNA organization is still underexplored. Here, we made use of DNA replication to directly label genomic DNA in an unbiased genome-wide manner. This was followed by live-cell time-lapse microscopy of the labeled DNA combining imaging at different resolutions levels simultaneously and allowing one to trace DNA motion across organization levels within the same cells. Quantification of the labeled DNA segments at different microscopic resolution levels revealed sizes comparable to the ones reported for DNA loops using 3D super-resolution microscopy, topologically associated domains (TAD) using 3D widefield microscopy, and also entire chromosomes. By employing advanced chromatin tracking and image registration, we discovered that DNA exhibited higher mobility at the individual loop level compared to the TAD level and even less at the chromosome level. Additionally, our findings indicate that chromatin movement, regardless of the resolution, slowed down during the S phase of the cell cycle compared to the G1/G2 phases. Furthermore, we found that a fraction of DNA loops and TADs exhibited directed movement with the majority depicting constrained movement. Our data also indicated spatial mobility differences with DNA loops and TADs at the nuclear periphery and the nuclear interior exhibiting lower velocity and radius of gyration than the intermediate locations. On the basis of these insights, we propose that there is a link between DNA mobility and its organizational structure including spatial distribution, which impacts cellular processes.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":" ","pages":"109-131"},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11227476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140957006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In focus in HCB. 重点关注 HCB。

IF 2.3 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-06-01 DOI: 10.1007/s00418-024-02299-5

Douglas J Taatjes, Jürgen Roth

引用次数: 0

Oxygen levels affect oviduct epithelium functions in air-liquid interface culture. 氧气水平影响气液界面培养中输卵管上皮细胞的功能

IF 2.3 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-06-01 Epub Date: 2024-03-26 DOI: 10.1007/s00418-024-02273-1

Jianchao Huo, Aleksandra Maria Mówińska, Ali Necmi Eren, Jennifer Schoen, Shuai Chen

{"title":"Oxygen levels affect oviduct epithelium functions in air-liquid interface culture.","authors":"Jianchao Huo, Aleksandra Maria Mówińska, Ali Necmi Eren, Jennifer Schoen, Shuai Chen","doi":"10.1007/s00418-024-02273-1","DOIUrl":"10.1007/s00418-024-02273-1","url":null,"abstract":"Key reproductive events such as fertilization and early embryonic development occur in the lumen of the oviduct. Since investigating these processes in vivo is both technically challenging and ethically sensitive, cell culture models have been established to reproduce the oviductal microenvironment. Compartmentalized culture systems, particularly air-liquid interface cultures (ALI; cells access the culture medium only from the basolateral cell side), result in highly differentiated oviduct epithelial cell cultures. The oxygen (O2) tension within the oviduct is 4-10% across species, and its reduced O2 content is presumed to be important for early reproductive processes. However, cell culture models of the oviduct are typically cultivated without O2 regulation and therefore at about 18% O2. To investigate the impact of O2 levels on oviduct epithelium functions in vitro, we cultured porcine oviduct epithelial cells (POEC) at the ALI using both physiological (5%) and supraphysiological (18%) O2 levels and two different media regimes. Epithelium architecture, barrier function, secretion of oviduct fluid surrogate (OFS), and marker gene expression were comparatively assessed. Under all culture conditions, ALI-POEC formed polarized, ciliated monolayers with appropriate barrier function. Exposure to 18% O2 accelerated epithelial differentiation and significantly increased the apical OFS volume and total protein content. Expression of oviduct genes and the abundance of OVGP1 (oviduct-specific glycoprotein 1) in the OFS were influenced by both O2 tension and medium choice. In conclusion, oviduct epithelial cells can adapt to a supraphysiological O2 environment. This adaptation, however, may alter their capability to replicate in vivo tissue characteristics.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":" ","pages":"521-537"},"PeriodicalIF":2.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11162385/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140293401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MOTS-c regulates pancreatic alpha and beta cell functions in vitro. MOTS-c 在体外调节胰腺α细胞和β细胞的功能。

IF 2.3 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-06-01 Epub Date: 2024-03-02 DOI: 10.1007/s00418-024-02274-0

Jakub Bień, Ewa Pruszyńska-Oszmałek, Paweł Kołodziejski, Natalia Leciejewska, Dawid Szczepankiewicz, Maciej Sassek

{"title":"MOTS-c regulates pancreatic alpha and beta cell functions in vitro.","authors":"Jakub Bień, Ewa Pruszyńska-Oszmałek, Paweł Kołodziejski, Natalia Leciejewska, Dawid Szczepankiewicz, Maciej Sassek","doi":"10.1007/s00418-024-02274-0","DOIUrl":"10.1007/s00418-024-02274-0","url":null,"abstract":"The aim of this study is to determine the influence of the mitochondrial open-reading-frame of the twelve S rRNA-c (MOTS-c) peptide on pancreatic cell physiology. Moreover, in this study, we examined the changes in MOTS-c secretion and expression under different conditions. Our experiments were conducted using laboratory cell line cultures, specifically the INS-1E and αTC-1 cell lines, which represent β and α pancreatic cells, respectively. As the pancreas is an endocrine organ, we also tested its hormone regulation capabilities. Furthermore, we assessed the secretion of MOTS-c after incubating the cells with glucose and free fatty acids. Additionally, we examined key cell culture parameters such as cell viability, proliferation, and apoptosis. The results obtained from this study show that MOTS-c has a significant impact on the physiology of pancreatic cells. Specifically, it lowers insulin secretion and expression in INS-1E cells and enhances glucagon secretion and expression in αTC-1 cells. Furthermore, MOTS-c affects cell viability and apoptosis. Interestingly, insulin and glucagon affect the MOTS-c secretion as well as glucose and free fatty acids. These experiments clearly show that MOTS-c is an important regulator of pancreatic metabolism, and there are numerous properties of MOTS-c yet to be discovered.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":" ","pages":"449-460"},"PeriodicalIF":2.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11162381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140012479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Wisteria floribunda agglutinin binding glycans in carcinogenesis and metastasis of cholangiocarcinoma. 紫藤花凝集素结合糖在胆管癌的癌变和转移中的作用

IF 2.3 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-05-01 Epub Date: 2024-02-23 DOI: 10.1007/s00418-024-02270-4

Winunya Phuyathip, Siyaporn Putthisen, Orasa Panawan, Prasertsri Ma-In, Karuntarat Teeravirote, Phisit Sintusen, Sirintra Udomkitkosol, Marutpong Detarya, Sukanya Luang, Panupong Mahalapbutr, Takashi Sato, Atsushi Kuno, Sriwipa Chuangchaiya, Atit Silsirivanit

{"title":"Role of Wisteria floribunda agglutinin binding glycans in carcinogenesis and metastasis of cholangiocarcinoma.","authors":"Winunya Phuyathip, Siyaporn Putthisen, Orasa Panawan, Prasertsri Ma-In, Karuntarat Teeravirote, Phisit Sintusen, Sirintra Udomkitkosol, Marutpong Detarya, Sukanya Luang, Panupong Mahalapbutr, Takashi Sato, Atsushi Kuno, Sriwipa Chuangchaiya, Atit Silsirivanit","doi":"10.1007/s00418-024-02270-4","DOIUrl":"10.1007/s00418-024-02270-4","url":null,"abstract":"Aberrant glycosylation is an important factor in facilitating tumor progression and therapeutic resistance. In this study, using Wisteria floribunda agglutinin (WFA), we examined the expression of WFA-binding glycans (WFAG) in cholangiocarcinoma (CCA). The results showed that WFAG was highly detected in precancerous and cancerous lesions of human CCA tissues, although it was rarely detected in normal bile ducts. The positive signal of WFAG in the cancerous lesion accounted for 96.2% (50/52) of the cases. Overexpression of WFAG was significantly associated with lymph node and distant metastasis (P < 0.05). The study using the CCA hamster model showed that WFAG is elevated in preneoplastic and neoplastic bile ducts as early as 1 month after being infected with liver fluke and exposed to N-nitrosodimethylamine. Functional analysis was performed to reveal the role of WFAG in CCA. The CCA cell lines KKU-213A and KKU-213B were treated with WFA, followed by migration assay. Our data suggested that WFAG facilitates the migration of CCA cells via the activation of the Akt and ERK signaling pathways. In conclusion, we have demonstrated the association of WFAG with carcinogenesis and metastasis of CCA, suggesting its potential as a target for the treatment of the disease.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":" ","pages":"423-434"},"PeriodicalIF":2.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139930936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Addressing radiotherapy-induced fibrosis: the potential of platelet-rich plasma and infliximab for improved breast cancer management. 应对放疗诱发的纤维化：富血小板血浆和英夫利昔单抗改善乳腺癌治疗的潜力。

IF 2.3 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-05-01 Epub Date: 2024-01-28 DOI: 10.1007/s00418-024-02267-z

Serhat Binici, Mustafa Güven, Abdulselam Özdemir, Zehra Akman İlik, Birhan Demirhan, Serhat Uygur, Ümit Haluk İliklerden

{"title":"Addressing radiotherapy-induced fibrosis: the potential of platelet-rich plasma and infliximab for improved breast cancer management.","authors":"Serhat Binici, Mustafa Güven, Abdulselam Özdemir, Zehra Akman İlik, Birhan Demirhan, Serhat Uygur, Ümit Haluk İliklerden","doi":"10.1007/s00418-024-02267-z","DOIUrl":"10.1007/s00418-024-02267-z","url":null,"abstract":"Breast cancer treatment encompasses various therapeutic modalities, including surgery, radiotherapy, and chemotherapy. Breast-conserving surgery has been an integral part of breast cancer management. However, radiotherapy, an important component of breast cancer management, can lead to complications, particularly fibrosis, affecting reconstructive surgery outcomes. We conducted an in vivo study using 48 female Wistar Albino rats, employing segmental mastectomy and radiotherapy to simulate post-mastectomy conditions. The rats were divided into six groups: control, mastectomy, mastectomy + radiotherapy, mastectomy + platelet-rich plasma (PRP) + radiotherapy, mastectomy + infliximab + radiotherapy, and mastectomy + infliximab + PRP + radiotherapy. Edema, hyperemia, inflammation, and fibrosis were assessed as indicators of tissue response. Histopathological analysis revealed that mastectomy + infliximab and mastectomy + infliximab + PRP groups showed significant reductions in fibrosis compared to other groups. Edema, hyperemia, and inflammation were also less severe in these groups compared to the control group. Radiotherapy-induced fibrosis is a major concern in breast reconstruction. Our study suggests that local PRP application and systemic infliximab administration, either alone or in combination, could mitigate the adverse effects of radiotherapy. This approach has the potential to improve reconstructive outcomes in patients undergoing or having the possibility to undergo radiotherapy. This is the first study showing the effectiveness of infliximab and PRP combination on wound healing. The provided experimental rat model might offer guidance for further research. This study provides insights into optimizing outcomes in reconstructive breast surgery, paving the way for further research and clinical studies.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":" ","pages":"381-389"},"PeriodicalIF":2.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139570232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of ANKRD1 in rhabdomyosarcoma cell lines: expression, localization, and proteasomal degradation. 横纹肌肉瘤细胞系中 ANKRD1 的分子特征：表达、定位和蛋白酶体降解。

IF 2.3 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-05-01 Epub Date: 2024-02-23 DOI: 10.1007/s00418-024-02272-2

Emilija Milosevic, Mirjana Novkovic, Vittoria Cenni, Alberto Bavelloni, Snezana Kojic, Jovana Jasnic

{"title":"Molecular characterization of ANKRD1 in rhabdomyosarcoma cell lines: expression, localization, and proteasomal degradation.","authors":"Emilija Milosevic, Mirjana Novkovic, Vittoria Cenni, Alberto Bavelloni, Snezana Kojic, Jovana Jasnic","doi":"10.1007/s00418-024-02272-2","DOIUrl":"10.1007/s00418-024-02272-2","url":null,"abstract":"Rhabdomyosarcoma (RMS) is the most common soft tissue malignancy in children and adolescents. Respecting the age of the patients and the tumor aggressiveness, investigation of the molecular mechanisms of RMS tumorigenesis is directed toward the identification of novel therapeutic targets. To contribute to a better understanding of the molecular pathology of RMS, we investigated ankyrin repeat domain 1 (ANKRD1), designated as a potential marker for differential diagnostics. In this study, we used three RMS cell lines (SJRH30, RD, and HS-729) to assess its expression profile, intracellular localization, and turnover. They express wild-type ANKRD1, as judged by the sequencing of the open reading frame. Each cell line expressed a different amount of ANKRD1 protein, although the transcript level was similar. According to western blot analysis, ANKRD1 protein was expressed at detectable levels in the SJRH30 and RD cells (SJRH30 > RD), but not in the HS-729, even after immunoprecipitation. Immunocytochemistry revealed nuclear and cytoplasmic localization of ANKRD1 in all examined cell lines. Moreover, the punctate pattern of ANKRD1 staining in the nuclei of RD and HS-729 cells overlapped with coilin, indicating its association with Cajal bodies. We have shown that RMS cells are not able to overexpress ANKRD1 protein, which can be attributed to its proteasomal degradation. The unsuccessful attempt to overexpress ANKRD1 in RMS cells indicates the possibility that its overexpression may have detrimental effects for RMS cells and opens a window for further research into its role in RMS pathogenesis and for potential therapeutic targeting.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":" ","pages":"435-444"},"PeriodicalIF":2.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139939947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0