Histochemistry and Cell Biology最新文献

Methodological framework for chromogenic mRNA detection using in situ hybridization chain reaction. 用原位杂交链式反应检测显色mRNA的方法框架。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2026-05-06 DOI: 10.1007/s00418-026-02482-w

Mitsuru Yashiro, Yousuke Tsuneoka, Yusuke Atsumi, Aki Makanae, Hiromasa Funato

{"title":"Methodological framework for chromogenic mRNA detection using in situ hybridization chain reaction.","authors":"Mitsuru Yashiro, Yousuke Tsuneoka, Yusuke Atsumi, Aki Makanae, Hiromasa Funato","doi":"10.1007/s00418-026-02482-w","DOIUrl":"10.1007/s00418-026-02482-w","url":null,"abstract":"In situ hybridization chain reaction (HCR) is a highly sensitive method for visualizing single-molecule messenger RNA (mRNA) using self-assembling hairpin DNA pairs. However, conventional in situ HCR is primarily optimized for fluorescence detection, limiting its applicability in routine pathology and in tissues with strong autofluorescence. In this study, we established a chromogenic in situ HCR protocol using short hairpin DNA that achieves sensitivity comparable to fluorescent in situ HCR. To achieve efficient amplification and avoid steric hindrance, we adopted an indirect labeling strategy in which hairpin DNA conjugated with haptens (biotin, digoxigenin, or fluorescein) is detected using enzyme-conjugated streptavidin or antibodies. We validated this approach across various mouse tissues, including the brain, kidney, and liver, and on both slide-mounted and free-floating sections. The protocol enabled the clear visualization of low-abundance transcripts such as Oxtr and Esr1. Furthermore, we demonstrated the versatility of this approach by performing duplex chromogenic staining for two mRNA targets and by combining in situ HCR with immunohistochemistry to visualize mRNA and protein simultaneously. This chromogenic in situ HCR retains the high sensitivity of HCR and adds the practical advantages of bright-field detection, thereby expanding the utility of in situ HCR in histological research.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"164 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13149615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147837305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Approaches to visualize, quantify, and manipulate phosphoinositides in cells. 细胞中磷酸肌苷可视化、量化和操作的方法。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2026-05-05 DOI: 10.1007/s00418-026-02479-5

Rabia Gönül, Agnieszka Chytła, Ana Miladinović, Ludovica Antiga, Pavel Hozák, Martin Sztacho

{"title":"Approaches to visualize, quantify, and manipulate phosphoinositides in cells.","authors":"Rabia Gönül, Agnieszka Chytła, Ana Miladinović, Ludovica Antiga, Pavel Hozák, Martin Sztacho","doi":"10.1007/s00418-026-02479-5","DOIUrl":"https://doi.org/10.1007/s00418-026-02479-5","url":null,"abstract":"Phosphoinositides are low-abundance regulatory lipids that control a broad range of cellular processes, from membrane trafficking and cytoskeletal remodeling to transcriptional regulation and RNA processing. These lipids are distributed across distinct subcellular compartments, where they carry out compartment-specific regulatory functions. Dysregulation of phosphoinositide metabolism is associated with cancer, neurodegenerative diseases, and immune dysfunction. However, their roles remain difficult to investigate owing to technical limitations in lipid detection and manipulation. This review outlines current strategies for modulating, visualizing, and quantifying phosphoinositide pools, including genetic manipulation techniques such as RNA interference, clustered regularly interspaced short palindromic repeats (CRISPR)-based approaches, and optogenetics. It also evaluates visualization tools such as fluorescent biosensors and live-cell imaging techniques, including superresolution microscopy. In parallel, quantitative methods such as thin-layer chromatography and mass spectrometry for profiling phosphoinositide species, including isomer- and acyl-specific variants, are discussed. By comparing the strengths and limitations of these approaches and highlighting how they can be combined, this review provides a practical framework for dissecting phosphoinositide function in defined subcellular contexts.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"164 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13144278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147837280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of obesity on DNA methylation and DNA methyltransferases in the reproductive system. 肥胖对生殖系统DNA甲基化和DNA甲基转移酶的影响。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2026-04-26 DOI: 10.1007/s00418-026-02481-x

Gözde Şükür, Özgür Çınar, Fatma Uysal Çınar

{"title":"Effects of obesity on DNA methylation and DNA methyltransferases in the reproductive system.","authors":"Gözde Şükür, Özgür Çınar, Fatma Uysal Çınar","doi":"10.1007/s00418-026-02481-x","DOIUrl":"https://doi.org/10.1007/s00418-026-02481-x","url":null,"abstract":"Obesity is increasingly recognized as a condition with profound systemic and epigenetic consequences. However, the molecular mechanisms linking obesity to reproductive dysfunction remain incompletely understood. Accumulating evidence suggests that epigenetic regulation, particularly DNA methylation, contributes to and is modified by obesity, thereby influencing gene expression in gonadal tissues and germ cells. DNA methylation is essential for normal oogenesis and spermatogenesis and is mediated by DNA methyltransferases (DNMTs), whose expression and activity are tightly regulated throughout germ cell development. This review synthesizes current experimental and clinical evidence regarding obesity-associated alterations in DNMT expression and DNA methylation patterns across female and male reproductive systems and examines how these epigenetic disruptions contribute to infertility. Obesity is most frequently associated with a hypermethylated epigenetic landscape in female reproductive tissues, whereas the male germline more commonly exhibits global or locus-specific hypomethylation, reflecting sex-specific epigenetic responses to metabolic stress. Studies spanning ovarian tissue, oocytes, sperm, and early embryos demonstrate that obesity-induced DNMT dysregulation and DNA methylation remodeling disrupt transcriptional programs governing folliculogenesis, spermatogenesis, and embryo development.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"164 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13111531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147769724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SASP-driven vascular aging: unraveling the transcriptional nexus in endothelial senescence and cardiovascular disease. sasp驱动的血管老化：揭示内皮衰老和心血管疾病的转录联系。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2026-04-25 DOI: 10.1007/s00418-026-02475-9

Shuqing Mao, Jian Cui

{"title":"SASP-driven vascular aging: unraveling the transcriptional nexus in endothelial senescence and cardiovascular disease.","authors":"Shuqing Mao, Jian Cui","doi":"10.1007/s00418-026-02475-9","DOIUrl":"https://doi.org/10.1007/s00418-026-02475-9","url":null,"abstract":"Endothelial cell senescence represents a critical mechanistic driver in the initiation and progression of cardiovascular diseases. Senescent endothelial cells exhibit characteristic features, including cell cycle arrest-mediated primarily through the p53/p21 and p16 pathways-morphological transformations such as increased cell volume, elevated caveolin-1 expression, and loss of LaminB1, as well as activation of the senescence-associated secretory phenotype (SASP). The SASP facilitates the secretion of numerous inflammatory cytokines and chemokines, thereby fostering a state of chronic inflammation and contributing to tissue dysfunction. Key molecular regulators of endothelial senescence include transcription factors such as NF-κB and p53, along with the p38 MAPK signaling pathway, which collectively modulate inflammatory responses, cell cycle progression, and stress adaptation. This review offers a comprehensive and integrative perspective on endothelial senescence as a central element in cardiovascular pathophysiology. Its novelty stems from a systematic synthesis of classical pathways, including p53/p21 and p16, with more recently implicated players such as mammalian target of rapamycin (mTOR) signaling and associated microRNAs (miRNAs), accompanied by a focused examination of the SASP as a core pathological mechanism in chronic inflammation and vascular impairment. Moving beyond singular pathways, this work constructs a multidimensional framework that integrates cell cycle arrest, morphological changes, SASP activation, and transcriptional regulation to delineate a cohesive pathological sequence through which endothelial senescence promotes cardiovascular disease.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"164 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147770042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Localization of dendritic cells and T cells within the tumor microenvironment in different types of skin cancer. 树突状细胞和T细胞在不同类型皮肤癌肿瘤微环境中的定位。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2026-04-25 DOI: 10.1007/s00418-026-02468-8

Marina Wanner, Anna Brunner, Daniela Ortner, Christoph H Tripp, Martin Hermann, Selina Neurauter, Barbara Del-Frari, Van Anh Nguyen, Patrizia Stoitzner

{"title":"Localization of dendritic cells and T cells within the tumor microenvironment in different types of skin cancer.","authors":"Marina Wanner, Anna Brunner, Daniela Ortner, Christoph H Tripp, Martin Hermann, Selina Neurauter, Barbara Del-Frari, Van Anh Nguyen, Patrizia Stoitzner","doi":"10.1007/s00418-026-02468-8","DOIUrl":"https://doi.org/10.1007/s00418-026-02468-8","url":null,"abstract":"Immunophenotyping of tumor-infiltrating immune cells is increasingly important for understanding the tumor microenvironment (TME), particularly in the diagnosis and treatment of skin cancer. CD1a+ dendritic cells (DC) initiate T cell activation by presenting tumor antigens, while T cells directly target tumor cells. Analyzing their spatial distribution in different types of skin cancer can provide insights into immune response patterns. To characterize the immune cell composition within the TME, we performed immunofluorescence staining for CD1a and CD3 on formalin-fixed, paraffin-embedded (FFPE) samples from actinic keratoses (n = 18), squamous cell carcinoma (n = 23), basal cell carcinoma (n = 19), and melanoma (n = 22), with nevi (n = 16) and healthy skin (n = 9) as controls. Immune cells were quantified across four tumor compartments: intratumoral, tumor margin, intraepidermal, and intradermal. Both CD1a+ DC and CD3+ T cells were detected across all tumor entities, displaying distinct spatial distribution patterns. DC were enriched intratumorally and within the epidermis, whereas T cells predominantly accumulated at the tumor margin (main effect of region, p < 0.001). Melanoma exhibited significantly fewer DC at the tumor margin while maintaining strong T cell infiltration. Overall, the immune architecture of skin tumors is highly compartmentalized, characterized by region-specific DC and T cell distributions. These findings underscore the relevance of spatial immune profiling for understanding immune escape mechanisms and informing immunotherapeutic strategies.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"164 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147769738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oncogenic overexpression of kallikrein-related peptidase 7 is associated with the progression of thyroid cancer. 致瘤性钾化钾素相关肽酶7的过表达与甲状腺癌的进展有关。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2026-04-22 DOI: 10.1007/s00418-026-02480-y

Shun-Yu Chi, Chi-Yu Kuo, Shih-Yuan Huang, Shih-Ping Cheng

{"title":"Oncogenic overexpression of kallikrein-related peptidase 7 is associated with the progression of thyroid cancer.","authors":"Shun-Yu Chi, Chi-Yu Kuo, Shih-Yuan Huang, Shih-Ping Cheng","doi":"10.1007/s00418-026-02480-y","DOIUrl":"https://doi.org/10.1007/s00418-026-02480-y","url":null,"abstract":"Mounting evidence suggests that the kallikrein-related peptidase family is dysregulated in thyroid cancer. However, previous analyses of kallikrein-related peptidase 7 (KLK7) expression patterns have yielded inconsistent results. In this study, KLK7 expression was examined in different histological types of thyroid cancer using immunohistochemistry. We found no detectable KLK7 immunoreactivity in the follicular epithelium of normal thyroid tissue or follicular adenomas. Weak KLK7 expression was observed in papillary (mean H-score 47) and follicular carcinomas (mean H-score 29), with moderate expression in anaplastic cancers (mean H-score 88; P for trend < 0.001). Membranous and cytoplasmic staining was validated using two independent antibodies and small interfering RNA (siRNA) knockdown controls. These observations were consistent with our bioinformatics analysis, which demonstrated that KLK7 was upregulated in less differentiated thyroid cancer and inversely correlated with thyroid differentiation scores. Knockdown of KLK7 expression through transfection with siRNA significantly impaired the viability, clonogenicity, and migratory abilities of thyroid cancer cells. This was accompanied by upregulation of E-cadherin and integrin-β1, along with elevated levels of the KLK7 substrates midkine and tenascin-C. Taken together, our results indicate that KLK7 is overexpressed in thyroid cancer and is linked to disease progression and dedifferentiation, partly by disrupting cell adhesion networks. KLK7 may therefore serve as a biomarker for aggressive thyroid cancer and a potential therapeutic target.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"164 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147769815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional suppression accompanies internal mitochondrial reorganization during cellular reprogramming. 在细胞重编程过程中，功能抑制伴随着线粒体内部重组。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2026-04-20 DOI: 10.1007/s00418-026-02472-y

N Diak, L Sieroń, K Janelt, L Chajec, A Fus-Kujawa, A Dziedzic-Kowalska, A Trybus, K Raszczok, K Bajdak-Rusinek

{"title":"Functional suppression accompanies internal mitochondrial reorganization during cellular reprogramming.","authors":"N Diak, L Sieroń, K Janelt, L Chajec, A Fus-Kujawa, A Dziedzic-Kowalska, A Trybus, K Raszczok, K Bajdak-Rusinek","doi":"10.1007/s00418-026-02472-y","DOIUrl":"10.1007/s00418-026-02472-y","url":null,"abstract":"During the conversion of fibroblasts into induced pluripotent stem cells (iPSCs), cellular metabolism shifts from oxidative phosphorylation toward glycolysis; however, how functional and structural mitochondrial adaptations are coordinated remains incompletely understood. Here, we examined selected aspects of mitochondrial function, targeted gene expression, and ultrastructure in fibroblasts and iPSCs derived from healthy donors and patients with osteogenesis imperfecta (OI). Targeted gene expression analysis was performed in all four cell types, while functional and ultrastructural assays focused on OI-derived cells. Flow cytometry revealed reduced mitochondrial mass and reactive oxygen species (ROS) levels in iPSCs compared with fibroblasts. Mitochondrial membrane potential showed modestly reduced fluorescence depending on the probe used. Quantitative transmission electron microscopy demonstrated internal mitochondrial reorganization in iPSCs, including altered morphology and simplified cristae architecture, despite preservation of overall membrane integrity. These findings indicate that reprogramming induces coordinated functional downscaling and structural remodeling of mitochondria. Furthermore, differential expression of matrix metalloproteinases suggests that extracellular matrix remodeling accompanies metabolic adaptation during reprogramming.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"164 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147728889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fiber type-specific expression of LACTB leverages a function in oxidative metabolism. 纤维类型特异性表达的LACTB在氧化代谢中发挥作用。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2026-04-18 DOI: 10.1007/s00418-026-02476-8

Kirsi-Marja Alanen, Rabah Soliymani, Jaakko Sarparanta, Satu Kuure, Kirsi Sainio, Zydrune Polianskyte, Muhammad Yasir Asghar, Ehsan Zangene, Annunziata Cascone, Maciej Lalowski, Peter Hackman, Johan Lundin, Dan Lindholm, Ove Eriksson

{"title":"Fiber type-specific expression of LACTB leverages a function in oxidative metabolism.","authors":"Kirsi-Marja Alanen, Rabah Soliymani, Jaakko Sarparanta, Satu Kuure, Kirsi Sainio, Zydrune Polianskyte, Muhammad Yasir Asghar, Ehsan Zangene, Annunziata Cascone, Maciej Lalowski, Peter Hackman, Johan Lundin, Dan Lindholm, Ove Eriksson","doi":"10.1007/s00418-026-02476-8","DOIUrl":"10.1007/s00418-026-02476-8","url":null,"abstract":"Skeletal muscle is composed of type I and type II fibers, each characterized by specific metabolic machinery. LACTB is a conserved mitochondrial protein implicated in lipid utilization and tumorigenesis, but its precise function in the cellular metabolism remains unclear. To gain novel insight into the functional role of LACTB, we investigated skeletal muscle to determine whether LACTB is segregated by fiber type. The expression of LACTB was determined by immunohistochemistry (IHC) and immunoblotting in skeletal muscle from healthy human subjects and the laboratory rat. The specificity of the antibody was assessed using recombinant human LACTB protein and endogenous LACTB in isolated mitochondria. IHC results were validated in a cellular model of myoblast differentiation using the C2C12 and L6 cell lines. The results demonstrated that LACTB is highly enriched in adult type I muscle fibers. During development, LACTB expression commences in type I primary myotubes at the time of their formation around week 20 of gestational age. LACTB expression in myoblasts is low but increases rapidly upon the induction of myotube differentiation. We conclude that LACTB plays a distinct role in mitochondria of type I fibers, most likely acting in oxidative metabolism related to energy use from lipids. This defines LACTB as a mitochondrial marker for type I fibers.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"164 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147716914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ER stress affects proliferation and induces polyploidization of cultured human HaCaT and A431 cells. 内质网应激影响培养的人HaCaT和A431细胞的增殖并诱导其多倍体化。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2026-04-13 DOI: 10.1007/s00418-026-02474-w

Ilia I Zakharov, Polina A Veselova, Margarita A Savitskaya, Elena A Smirnova, Galina E Onishchenko

{"title":"ER stress affects proliferation and induces polyploidization of cultured human HaCaT and A431 cells.","authors":"Ilia I Zakharov, Polina A Veselova, Margarita A Savitskaya, Elena A Smirnova, Galina E Onishchenko","doi":"10.1007/s00418-026-02474-w","DOIUrl":"https://doi.org/10.1007/s00418-026-02474-w","url":null,"abstract":"Endoplasmic reticulum (ER) stress is the accumulation of misfolded or defective proteins in the ER. ER stress is capable of inducing both anti-apoptotic and pro-apoptotic cellular response, and at the same time plays an important role in metabolism and progression of many types of tumors. Current understanding of the role of ER stress in changing functional parameters of normal and tumor cells is lacking. This study investigated how ER stress inducers bortezomib, dithiothreitol, and tunicamycin influence proliferation, cell cycle, and changes in ploidy of normal and tumor cells of epidermal origin HaCaT and A431 in vitro following incubation with the agent as well as after its removal from the culture medium. Bortezomib caused a cell cycle arrest in the G2 phase in HaCaT cells, as well as polyploidization in both cell lines. Dithiothreitol induced apoptosis in HaCaT cells. Tunicamycin caused a decrease in proliferative index, cell cycle arrest, as well as apoptosis and necrosis in the A431 cells. In conclusion, induction of ER stress by different mechanisms has different effects on normal and tumor cells and can lead to both polyploidization and, presumably, cell differentiation or senescence.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"164 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147672753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Periostin in joint physiology and pathology: multiple roles from mechanisms to clinical perspectives. 骨膜素在关节生理病理中的作用：从机制到临床的多重作用。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2026-04-08 DOI: 10.1007/s00418-026-02469-7

Qiaoli Dai, Zuping Wu, Ying Wang, Na Wu, Chenyu Wang, Jiejun Shi

{"title":"Periostin in joint physiology and pathology: multiple roles from mechanisms to clinical perspectives.","authors":"Qiaoli Dai, Zuping Wu, Ying Wang, Na Wu, Chenyu Wang, Jiejun Shi","doi":"10.1007/s00418-026-02469-7","DOIUrl":"https://doi.org/10.1007/s00418-026-02469-7","url":null,"abstract":"Joint-related diseases, including osteoarthritis (OA), rheumatoid arthritis (RA), intervertebral disc degeneration (IDD), anterior cruciate ligament (ACL) injury, and shoulder instability, severely compromise human health. Periostin (POSTN), a multifunctional extracellular matrix protein enriched in connective tissues, exerts multifaceted roles in joint biology. It supports normal tissue development, but under pathological conditions, it binds to cell-surface receptors. This interaction activates downstream signaling cascades, such as Wnt/β-catenin, nuclear factor κB (NF-κB), and transforming growth factor β (TGF-β), thereby propelling disease progression. Notably, POSTN's functional specificity is dictated by its splice variant profiles and cellular localization, with the tissue-specific expression and functional divergence of these variants emerging as a pivotal research focus. Recent evidence links POSTN expression levels to the severity of joint-related diseases, highlighting its potential as a diagnostic biomarker. This review summarizes the core roles and underlying mechanisms of POSTN in joint physiology and pathology, laying a theoretical foundation for clinical translation.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"164 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147633096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0