Histochemistry and Cell Biology最新文献

Immunohistochemical analysis and distribution of epithelial mast cells in the rat larynx and trachea. 大鼠喉和气管上皮肥大细胞的免疫组化分析和分布。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-10-01 Epub Date: 2024-07-20 DOI: 10.1007/s00418-024-02309-6

Sayed Sharif Abdali, Takuya Yokoyama, Yoshio Yamamoto, Keishi Narita, Masato Hirakawa, Tomoyuki Saino

{"title":"Immunohistochemical analysis and distribution of epithelial mast cells in the rat larynx and trachea.","authors":"Sayed Sharif Abdali, Takuya Yokoyama, Yoshio Yamamoto, Keishi Narita, Masato Hirakawa, Tomoyuki Saino","doi":"10.1007/s00418-024-02309-6","DOIUrl":"10.1007/s00418-024-02309-6","url":null,"abstract":"Mast cells (MCs) in rat airways have been classified into two subtypes: epithelial MCs and connective tissue MCs (CTMCs). However, the immunohistochemical characteristics, cellular morphology, and distribution of epithelial MCs in the upper airways remain unclear. The present study investigated the morphological characteristics and distribution of epithelial MCs using 5-hydroxytryptamine (5-HT) and other immunohistochemical markers in sectioned or whole-mount preparations of the rat larynx and trachea. A double immunofluorescence analysis revealed the colocalization of 5-HT immunoreactivity with c-kit, a stem cell factor receptor commonly used as a MC marker, in both epithelial MCs and CTMCs. Dopa decarboxylase, an enzyme involved in 5-HT synthesis, was detected in both subtypes, suggesting their ability to synthesize and release 5-HT. Tryptase and histidine decarboxylase (a biosynthetic enzyme of histamine), which are well-known mediators of MCs, were exclusive to CTMCs. Epithelial MCs were pleomorphic with long cytoplasmic processes, whereas CTMCs were round and lacked cytoplasmic processes. The density of epithelial MCs was significantly higher in the glottis and cranial part of the trachea than in the epiglottis and other parts of the trachea. The present results showed that the morphology and immunohistochemical characteristics of epithelial MCs were different from those of CTMCs in the rat larynx and trachea, and variform epithelial MCs were predominantly located at the entrance of the upper airways. Epithelial MCs may release 5-HT to regulate innate immune responses by modulating epithelial cell functions at the entrance gate of the upper airways.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141731120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Short and long-term 2100 MHz radiofrequency radiation causes endoplasmic reticulum stress in rat testis. 短期和长期 2100 兆赫射频辐射导致大鼠睾丸内质网应激。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-10-01 Epub Date: 2024-07-12 DOI: 10.1007/s00418-024-02308-7

Esma Kirimlioglu, Asli Okan Oflamaz, Enis Hidisoglu, Sukru Ozen, Piraye Yargicoglu, Necdet Demir

{"title":"Short and long-term 2100 MHz radiofrequency radiation causes endoplasmic reticulum stress in rat testis.","authors":"Esma Kirimlioglu, Asli Okan Oflamaz, Enis Hidisoglu, Sukru Ozen, Piraye Yargicoglu, Necdet Demir","doi":"10.1007/s00418-024-02308-7","DOIUrl":"10.1007/s00418-024-02308-7","url":null,"abstract":"Long-term radiofrequency radiation (RFR) exposure, which adversely affects organisms, deteriorates testicular functions. Misfolding or unfolding protein accumulation in the endoplasmic reticulum (ER) initiates an intracellular reaction known as ER stress (ERS), which activates the unfolded protein response (UPR) for proteostasis. Since both RFR exposure and ERS can cause male infertility, we hypothesized that RFR exposure causes ERS to adversely affect testicular functions in rats. To investigate role of ERS in mediating RFR effects on rat testis, we established five experimental groups in male rats: control, short-term 2100-megahertz (MHz) RFR (1-week), short-term sham (sham/1-week), long-term 2100-MHz RFR (10-week), and long-term sham (sham/10-week). ERS markers Grp78 and phosphorylated PERK (p-Perk) levels and ERS-related apoptosis markers Chop and caspase 12 were investigated by immunohistochemistry, immunoblotting, and quantitative real-time polymerase chain reaction (qPCR). Long-term RFR exposure increased Grp78, p-Perk, and Chop levels, while short-term RFR exposure elevated Chop and caspase 12 levels. Chop expression was not observed in spermatogonia and primary spermatocytes, which may protect spermatogonia and primary spermatocytes against RFR-induced ERS-mediated apoptosis, thereby allowing transmission of genetic material to next generations. While short and long-term RFR exposures trigger ERS and ERS-related apoptotic pathways, further functional analyses are needed to elucidate whether this RFR-induced apoptosis has long-term male infertility effects.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141599221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glyoxal-methyl-ethylene sulfonic acid fixative enhances the fixation of cytoskeletal structures for Förster resonance energy transfer measurements. 乙二醛-甲基-乙烯磺酸固定剂增强了用于福斯特共振能量转移测量的细胞骨架结构的固定。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-10-01 Epub Date: 2024-06-17 DOI: 10.1007/s00418-024-02304-x

Sei Kuriyama, Kuboki Thasaneeya, Go Itoh, Satoru Kidoaki, Masamitsu Tanaka

{"title":"Glyoxal-methyl-ethylene sulfonic acid fixative enhances the fixation of cytoskeletal structures for Förster resonance energy transfer measurements.","authors":"Sei Kuriyama, Kuboki Thasaneeya, Go Itoh, Satoru Kidoaki, Masamitsu Tanaka","doi":"10.1007/s00418-024-02304-x","DOIUrl":"10.1007/s00418-024-02304-x","url":null,"abstract":"Förster resonance energy transfer (FRET) serves as a tool for measuring protein-protein interactions using various sensor molecules. The tension sensor module relies on FRET technology. In our study, this module was inserted within the actinin molecule to measure the surface tension of the cells. Given that the decay curve of FRET efficiency correlates with surface tension increase, precise and accurate efficiency measurement becomes crucial. Among the methods of FRET measurements, FRET efficiency remains the most accurate if sample fixation is successful. However, when cells were fixed with 4% paraformaldehyde (PFA), the actinin-FRET sensor diffused across the cytoplasm; this prompted us to explore fixation method enhancements. Glyoxal fixative has been reported to improve cytoskeletal morphologies compared to PFA. However, it was not known whether glyoxal fits FRET measurements. Glyoxal necessitates an acetic acid solution for fixation; however, acidic conditions could compromise fluorescence stability. We observed that the pH working range of glyoxal fixative aligns closely with MES (methyl-ethylene sulfonic acid) Good's buffer. Initially, we switched the acidic solution for MES buffer and optimized the fixation procedure for in vitro and in vivo FRET imaging. By comparing FRET measurements on hydrogels with known stiffness to tumor nodules in mouse lung, we estimated in vivo stiffness. The estimated stiffness of cancerous tissue was harder than the reported stiffness of smooth muscle. This discovery shed lights on how cancer cells perceive environmental stiffness during metastasis.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141330780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in human skin composition due to intrinsic aging: a histologic and morphometric study. 内在衰老导致的人体皮肤成分变化：组织学和形态计量学研究。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-10-01 Epub Date: 2024-07-02 DOI: 10.1007/s00418-024-02305-w

Marta Arnal-Forné, Tamara Molina-García, María Ortega, Víctor Marcos-Garcés, Pilar Molina, Antonio Ferrández-Izquierdo, Pilar Sepulveda, Vicente Bodí, César Ríos-Navarro, Amparo Ruiz-Saurí

{"title":"Changes in human skin composition due to intrinsic aging: a histologic and morphometric study.","authors":"Marta Arnal-Forné, Tamara Molina-García, María Ortega, Víctor Marcos-Garcés, Pilar Molina, Antonio Ferrández-Izquierdo, Pilar Sepulveda, Vicente Bodí, César Ríos-Navarro, Amparo Ruiz-Saurí","doi":"10.1007/s00418-024-02305-w","DOIUrl":"10.1007/s00418-024-02305-w","url":null,"abstract":"Skin represents the main barrier against the external environment, but also plays a role in human relations, as one of the prime determinants of beauty, resulting in a high consumer demand for skincare-related pharmaceutical products. Given the importance of skin aging in both medical and social spheres, the present research aims to characterize microscopic changes in human skin composition due to intrinsic aging (as opposed to aging influenced by external factors) via histological analysis of a photoprotected body region. Samples from 25 autopsies were taken from the periumbilical area and classified into four age groups: group 1 (0-12 years), group 2 (13-25 years), group 3 (26-54 years), and group 4 (≥ 55 years). Different traditional histological (hematoxylin-eosin, Masson's trichrome, orcein, toluidine, Alcian blue, and Feulgen reaction) and immunohistochemical (CK20, CD1a, Ki67, and CD31) stains were performed. A total of 1879 images photographed with a Leica DM3000 optical microscope were morphometrically analyzed using Image ProPlus 7.0 for further statistical analysis with GraphPad 9.0. Our results showed a reduction in epidermis thickness, interdigitation and mitotic indexes, while melanocyte count was raised. Papillary but not reticular dermis showed increased thickness with aging. Specifically, in the papillary layer mast cells and glycosaminoglycans were expanded, whereas the reticular dermis displayed a diminution in glycosaminoglycans and elastic fibers. Moreover, total cellularity and vascularization of both dermises were diminished with aging. This morphometric analysis of photoprotected areas reveals that intrinsic aging significantly influences human skin composition. This study paves the way for further research into the molecular basis underpinning these alterations, and into potential antiaging strategies.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364716/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stage-specific expression of Toll-like receptors in the seminiferous epithelium of mouse testis. 小鼠睾丸曲细精管上皮细胞中 Toll 样受体的特异性表达。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-10-01 Epub Date: 2024-07-31 DOI: 10.1007/s00418-024-02310-z

Göksel Doğan, Mustafa Sandıkçı, Levent Karagenç

{"title":"Stage-specific expression of Toll-like receptors in the seminiferous epithelium of mouse testis.","authors":"Göksel Doğan, Mustafa Sandıkçı, Levent Karagenç","doi":"10.1007/s00418-024-02310-z","DOIUrl":"10.1007/s00418-024-02310-z","url":null,"abstract":"Genes encoding Toll-like receptors (TLRs) are expressed by germ cells in the mouse testis. Nevertheless, the expression of TLRs by germ cells has only been demonstrated for TLR-3, TLR-9, and TLR-11. Furthermore, the expression of each TLR in relation to the stage of spermatogenesis remains uncertain. We aimed in the present study to examine the expression pattern of all TLRs in germ cells throughout the cycle of seminiferous epithelium in the adult mouse testis. Immunohistochemistry was used to evaluate the expression of TLRs. Results of the present study reveal the expression of TLRs by specific populations of germ cells. Expression of TLRs, except for TLR-7, at endosomal compartments, acrosomes, and/or residual bodies was another interesting and novel finding of the present study. We further demonstrate that the expression of TLR-1, -2, -3, -4, -5, -7, -11, -12, and -13 follows a distinct spatiotemporal pattern throughout the cycle of seminiferous epithelium. While TLR-1, -3, -5, -11, and -12 are expressed in all stages, TLR-4 is expressed only in early and middle stages of spermatogenic cycle. On the other hand, TLR-2, -7, and -13 are expressed only in early stage of spermatogenic cycle. Evidence demonstrating the expression of TLRs in a stage specific manner throughout spermatogenesis strengthen the hypothesis that the expression of various TLRs by germ cells is a developmentally regulated process. However, if TLRs play a role in the regulation of proliferation, growth, maturation, and differentiation of germ cells throughout the cycle of the seminiferous epithelium warrants further investigations.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364606/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studies on the effect of curcumin and quercetin in the liver of male albino rats exposed to gamma irradiation. 姜黄素和槲皮素对遭受伽马射线照射的雄性白化大鼠肝脏影响的研究。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-10-01 Epub Date: 2024-06-24 DOI: 10.1007/s00418-024-02300-1

Amr M Abd El-Hady, Rady M Azzoz, Saeed M Soliman, Ibrahim Y Abdelrahman, Wafaa M Khalil, Said A Ali

{"title":"Studies on the effect of curcumin and quercetin in the liver of male albino rats exposed to gamma irradiation.","authors":"Amr M Abd El-Hady, Rady M Azzoz, Saeed M Soliman, Ibrahim Y Abdelrahman, Wafaa M Khalil, Said A Ali","doi":"10.1007/s00418-024-02300-1","DOIUrl":"10.1007/s00418-024-02300-1","url":null,"abstract":"Ionizing radiation produces deleterious effects on living organisms. The present investigation has been carried out to study the prophylactic as well as the therapeutic effects of treated rats with quercetin (Quer) and curcumin (Cur), which are two medicinal herbs known for their antioxidant activities against damages induced by whole-body fractionated gamma irradiation. Exposure of rats to whole-body gamma irradiation induced a significant decrease in erythrocyte (RBC), leukocyte (WBCs), platelet count (Plt), hemoglobin concentration (Hb), hematocrit (Hct %), mean erythrocyte hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and mean erythrocyte volume (MCV); a high increase in plasma thiobarbituric acid reactive substances (TBARS); a nonsignificant statistical decrease in the mean value of serum glutathione (GSH); a significant increase in plasma alanine transferase (ALT), aspartate transferase (AST), alkaline phosphates (ALP), serum total protein, serum total cholesterol levels, total triglycerides levels, high-density lipoprotein (HDL), and low-density lipoprotein (LDL) levels; and with marked histological changes and structural changes measured by Fourier transform infrared (FTIR). Applying both quercetin and curcumin pre- and postexposure to gamma radiation revealed a remarkable improvement in all the studied parameters. The cellular damage by gamma radiation is greatly mitigated by the coadministration of curcumin and quercetin before radiation exposure.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Placental neutrophil reverse trans-migration and maternal serum neutrophil extracellular trap expression in HIV infection co-morbid pre-eclampsia in women of African ancestry. 非洲裔妇女感染艾滋病病毒合并先兆子痫时胎盘中性粒细胞反向迁移和母体血清中性粒细胞胞外捕获器的表达。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-10-01 Epub Date: 2024-06-24 DOI: 10.1007/s00418-024-02298-6

Merantha Moodley, Jagidesa Moodley, Thajasvarie Naicker

{"title":"Placental neutrophil reverse trans-migration and maternal serum neutrophil extracellular trap expression in HIV infection co-morbid pre-eclampsia in women of African ancestry.","authors":"Merantha Moodley, Jagidesa Moodley, Thajasvarie Naicker","doi":"10.1007/s00418-024-02298-6","DOIUrl":"10.1007/s00418-024-02298-6","url":null,"abstract":"Neutrophil extracellular traps (NETs) and placental neutrophil reverse transmigration (r-TM) are implicated in the pathogenesis of pre-eclampsia (PE). However, the role of the comorbidity of PE and human immunodeficiency virus (HIV) infection in placental neutrophil r-TM and serum NETs remains unknown. Human placental tissue (n = 160) and serum (n = 80) samples were obtained post-ethical approval and divided by pregnancy type and HIV status and across the study population. Immunohistochemistry and morphometry were performed to localize and quantify junctional adhesion molecule-C (JAM-C) expression as an inverse marker of neutrophil r-TM within placental villi. An enzyme-linked immunosorbent assay (ELISA) was performed to quantify the concentration of citrullinated histone H3 (cit-H3) as a marker of NETs. GraphPad Prism (version 8.0.2) was used to compare the results, and a p value of p < 0.05 was considered statistically significant. The localization of JAM-C was observed on the syncytiotrophoblasts (STBs) and endothelial cells of placental villi. The immunoexpression of JAM-C was elevated in PE vs. normotensive (N) placentae. In the exchange villi, JAM-C immunoexpression was higher in the N+ve vs. N-ve group. However, in PE comorbid HIV infection, JAM-C expression was lower in the PE+ve vs. PE-ve group. Citrullinated histone-H3 concentration was lower in the N+ve vs. N-ve group but elevated in early-onset PE (EOPE)+ve vs. late-onset PE (LOPE)+ve group. These results indicate that PE and HIV-infected placentae individually express elevated JAM-C, manifesting in less neutrophil r-TM. However, in exchange villi of PE comorbid with HIV infection reduced JAM-C enhances neutrophil r-TM, thus supporting the synergistic effect of PE comorbid with HIV.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and functional testing of a novel in vitro delayed scratch closure assay. 新型体外延迟划痕闭合试验的开发和功能测试。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-09-01 Epub Date: 2024-05-07 DOI: 10.1007/s00418-024-02292-y

Yi Bing Aw, Sixun Chen, Aimin Yeo, John A Dangerfield, Pamela Mok

{"title":"Development and functional testing of a novel in vitro delayed scratch closure assay.","authors":"Yi Bing Aw, Sixun Chen, Aimin Yeo, John A Dangerfield, Pamela Mok","doi":"10.1007/s00418-024-02292-y","DOIUrl":"10.1007/s00418-024-02292-y","url":null,"abstract":"As the development of chronic wound therapeutics continues to expand, the demand for advanced assay systems mimicking the inflammatory wound microenvironment in vivo increases. Currently, this is performed in animal models or in in vitro cell-based models such as cell culture scratch assays that more closely resemble acute wounds. Here, we describe for the first time a delayed scratch closure model that mimics some features of a chronic wound in vitro. Chronic wounds such as those suffered by later stage diabetic patients are characterised by degrees of slowness to heal caused by a combination of continued localised physical trauma and pro-inflammatory signalling at the wound. To recreate this in a cell-based assay, a defined physical scratch was created and stimulated by combinations of pro-inflammatory factors, namely interferon, the phorbol ester PMA, and lipopolysaccharide, to delay scratch closure. The concentrations of these factors were characterised for commonly used human keratinocyte (HaCaT) and dermal fibroblast (HDF) cell lines. These models were then tested for scratch closure responsiveness to a proprietary healing secretome derived from human Wharton's jelly mesenchymal stem cells (MSCs) previously validated and shown to be highly effective on closure of acute wound models both in vitro and in vivo. The chronically open scratches from HaCaT cells showed closure after exposure to the MSC secretome product. We propose this delayed scratch closure model for academic and industrial researchers studying chronic wounds looking for responsiveness to drugs or biological treatments prior to testing on explanted patient material or in vivo.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11322216/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140848096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In focus in HCB. 重点关注 HCB。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-09-01 DOI: 10.1007/s00418-024-02315-8

Douglas J Taatjes, Jürgen Roth

引用次数: 0

Differential expression of small bowel TGFβ1 and TGFβ3 characterizes intestinal strictures in patients with fibrostenotic Crohn's disease. 小肠 TGFβ1 和 TGFβ3 的差异表达是纤维性克罗恩病患者肠道狭窄的特征。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-09-01 Epub Date: 2024-05-05 DOI: 10.1007/s00418-024-02290-0

Steven Levitte, Ibaad Khan, Violet Iyahen, James Ziai, John Gubatan, Rebecca Sheng, Sara B Glickstein, Tianhe Sun, K T Park, Jacqueline McBride, Mary Keir

{"title":"Differential expression of small bowel TGFβ1 and TGFβ3 characterizes intestinal strictures in patients with fibrostenotic Crohn's disease.","authors":"Steven Levitte, Ibaad Khan, Violet Iyahen, James Ziai, John Gubatan, Rebecca Sheng, Sara B Glickstein, Tianhe Sun, K T Park, Jacqueline McBride, Mary Keir","doi":"10.1007/s00418-024-02290-0","DOIUrl":"10.1007/s00418-024-02290-0","url":null,"abstract":"Small bowel strictures remain a debilitating consequence of Crohn's disease and contribute to poor outcomes for patients. Recently, TGFβ has been identified as an important driver of intestinal fibrosis. We studied the localization of TGFβ isoforms in ileal strictures of patients with Crohn's disease using in situ hybridization to understand TGFβ's role in stricture formation. The mucosa of strictures was characterized by higher TGFβ1 while the stricture submucosa showed higher TGFβ3 compared to normal ileum from patients without Crohn's disease (p = 0.02 and p = 0.044, respectively). We correlated these findings with single-cell transcriptomics which demonstrated that TGFβ3 transcripts overall are very rare, which may partially explain why its role in intestinal fibrosis has remained unclear to date. There were no significant differences in fibroblast or B cell TGFβ1 and/or TGFβ3 expression in inflamed vs. noninflamed ileum. We discuss the implications of these findings for therapeutic development strategies to treat patients with fibrostenotic Crohn's disease.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0