Histochemistry and Cell Biology最新文献

Microtubule nucleation at the Golgi-related membrane organelles of mouse neurons. 小鼠神经元高尔基相关膜细胞器的微管成核。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2025-05-28 DOI: 10.1007/s00418-025-02390-5

Haruki Kasai, Kensuke Hayashi

{"title":"Microtubule nucleation at the Golgi-related membrane organelles of mouse neurons.","authors":"Haruki Kasai, Kensuke Hayashi","doi":"10.1007/s00418-025-02390-5","DOIUrl":"https://doi.org/10.1007/s00418-025-02390-5","url":null,"abstract":"Microtubules are often nucleated at non-centrosomal sites in some differentiated cell types. We previously reported that microtubules are nucleated at the cytoplasm in cultured mouse cortical neurons. It is unclear, however, what organelle is the site of such nucleation. In this study, we examined the possibility that recently discovered neuron-specific Golgi-like structures called Golgi satellites are the nucleation sites. Microtubule nucleation was tested by observing microtubule regrowth after nocodazole depolymerization. First, the spatial association between microtubule nucleation and membrane organelles was investigated. Organelle markers including GM130 (cis Golgi and Golgi outpost marker), Golgin97 (trans-Golgi network marker), transferrin receptor (recycling endosome marker), TOMM40 (mitochondria marker), and syntaxin 6 (early endosome and Golgi satellite marker) were examined. Microtubule regrowth was observed in cytoplasmic regions where TOMM40-positive and syntaxin 6-positive organelles were rich. Triple immunostaining showed that γ-tubulin at one end of regrown microtubules was attached to syntaxin 6-organelles but not to TOMM40-organelles, indicating that syntaxin 6-organelles are the microtubule nucleation sites. To address the possibility that the microtubule-nucleating syntaxin 6-organelles were Golgi satellites, we transfected neurons with plasmid vector caring FLAG-tagged Golt sequence, a marker for Golgi satellites, and subsequently performed microtubule regrowth experiments. We found regrown microtubules on FLAG-positive organelles in dendrites. This observation suggests that Golgi satellites are microtubule nucleation sites. Microtubules from the Golgi satellites might guide transport vesicles generated at rough endoplasmic reticulum in dendrites.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"61"},"PeriodicalIF":2.1,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144158361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MST1 modulates inflammatory responses by targeting the NF-κB/NLRP3 pathway in LPS-induced acute lung injury. 在lps诱导的急性肺损伤中，MST1通过靶向NF-κB/NLRP3通路调节炎症反应。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2025-05-28 DOI: 10.1007/s00418-025-02383-4

Zi-Xi Peng, Lin-Li Song, Xiao-Li Wang

{"title":"MST1 modulates inflammatory responses by targeting the NF-κB/NLRP3 pathway in LPS-induced acute lung injury.","authors":"Zi-Xi Peng, Lin-Li Song, Xiao-Li Wang","doi":"10.1007/s00418-025-02383-4","DOIUrl":"https://doi.org/10.1007/s00418-025-02383-4","url":null,"abstract":"Acute lung injury (ALI) represents a severe respiratory condition. Inflammation is a pivotal factor in the pathogenesis of ALI. Mammalian STE20-like protein kinase 1 (MST1) has emerged as a key regulator of sphingolipid metabolism and a mediator of inflammatory responses. However, the precise role and underlying mechanisms of MST1 in lipopolysaccharide (LPS)-induced ALI remain unclear. This study aimed to investigate the influence of MST1 on the inflammatory response in LPS-induced ALI. An LPS-induced ALI model was established using RAW 264.7 cells and mice. In vivo, lung histopathological changes, wet-to-dry (W/D) weight ratio, myeloperoxidase (MPO) activity, and inflammatory cytokine levels [interleukin-1β (IL-1β) and tumor necrosis factor-alpha (TNF-α)] were assessed. Oxidative stress markers, including superoxide dismutase (SOD) activity and malondialdehyde (MDA) content, were measured. In vitro, MST1 overexpression was induced in RAW 264.7 cells via lentiviral transfection. Cell viability and proliferation were evaluated using the CCK-8 assay. The expression levels of NF-κB and NLRP3 signaling pathways in vitro or in vivo were analyzed using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) and western blotting analysis. The results showed that inhibition of MST1 expression attenuated lung damage, alleviated inflammation, enhanced antioxidant capacity, and inhibited the activation of NF-κB and NLRP3 pathways in vivo. In contrast, MST1 overexpression promoted cell proliferation and inflammation in vitro, accompanied by the activation of NF-κB and NLRP3 signaling pathways. This study demonstrated that MST1 activation contributed to inflammation in LPS-induced ALI by modulating the NF-κB/NLRP3 signaling pathway. Targeting MST1 may represent a novel approach to the treatment of ALI.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"60"},"PeriodicalIF":2.1,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144158377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

April In focus in HCB. 四月是HCB的焦点。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2025-05-28 DOI: 10.1007/s00418-025-02388-z

Douglas J Taatjes, Jürgen Roth

引用次数: 0

Tissue-specific localization of the ING4 targeting subunit of the HBO1 histone acetyltransferase in the cytoplasm and nucleus of secretory cells. 分泌细胞细胞质和细胞核中HBO1组蛋白乙酰转移酶ING4靶向亚基的组织特异性定位。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2025-05-22 DOI: 10.1007/s00418-025-02385-2

Arthur Dantas, Buthaina Al Shueili, Jeongah Park, Suleyman Abdullah, Jessica Bertschmann, Hokan Krowicki, Mahbod Djamshidi, Yang Yang, Karen Blote, Subhash Thalappilly, Karl Riabowol

{"title":"Tissue-specific localization of the ING4 targeting subunit of the HBO1 histone acetyltransferase in the cytoplasm and nucleus of secretory cells.","authors":"Arthur Dantas, Buthaina Al Shueili, Jeongah Park, Suleyman Abdullah, Jessica Bertschmann, Hokan Krowicki, Mahbod Djamshidi, Yang Yang, Karen Blote, Subhash Thalappilly, Karl Riabowol","doi":"10.1007/s00418-025-02385-2","DOIUrl":"10.1007/s00418-025-02385-2","url":null,"abstract":"Members of the INhibitor of Growth protein family (ING1-5) function as epigenetic regulators by targeting different histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes to the H3K4Me3 mark of active transcription. The INGs recognize H3K4Me3 by specific interaction with their well-conserved plant homeodomains, and affinity can be increased by interactions between DNA and disordered regions within the ING proteins. They are classified as type II tumor suppressors since they are downregulated in numerous cancer types and knockout of ING family members results in tumorigenesis. ING4 targets the HBO1 HAT complex, which is known to affect acetylation of the H4 core nucleosomal histone, to affect local chromatin structure and knockout results in deficient innate immunity. Reports indicating roles in cell cycle regulation, tumor suppression, and apoptosis suggest that ING4 may be a promising target for cancer treatment by targeting pathways of innate immunity. Given the relatedness between ING4 and the closely related ING5 proteins, we have developed and characterized two mouse monoclonal antibodies to specifically recognize human and mouse ING4, but not ING5, to more accurately characterize ING4 levels by western, immunofluorescence and immunohistochemical assays. Using them, we show that ING4 differentially partitions between the nucleus and cytoplasm in different tissues and localizes largely to the cytoplasm of cells having a secretory role in different tissue types.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"56"},"PeriodicalIF":2.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098486/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dysregulation of AR and ERα caused ovarian alterations in gerbils prenatally exposed to 17α-ethinylestradiol and pequi oil. 妊娠期暴露于17α-炔雌醇和贝基油的沙鼠卵巢发生AR和ERα的失调。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2025-05-22 DOI: 10.1007/s00418-025-02389-y

Amanda M de Campos, Jordana S Dias, Gabriel F Lopes, Thaisla M Pires, Daniele C da Silva, Thalles F Rocha Ruiz, Tracy M M Martins, Ana P S Perez

{"title":"Dysregulation of AR and ERα caused ovarian alterations in gerbils prenatally exposed to 17α-ethinylestradiol and pequi oil.","authors":"Amanda M de Campos, Jordana S Dias, Gabriel F Lopes, Thaisla M Pires, Daniele C da Silva, Thalles F Rocha Ruiz, Tracy M M Martins, Ana P S Perez","doi":"10.1007/s00418-025-02389-y","DOIUrl":"https://doi.org/10.1007/s00418-025-02389-y","url":null,"abstract":"This study investigated the effects of prenatal exposure to pequi oil and 17α-ethinylestradiol (EE2) on the histomorphometry and receptor expression (androgen receptor (AR) and estrogen receptor alpha (ERα) in gerbil ovaries (Meriones unguiculatus) during aging. Experimental groups included: control, vehicle (mineral oil), EE2: 15 µg/kg/day from gestational days 18-22, EE2/Pe: EE2 from days 18-22 and 300 mg/kg of pequi oil from days 18-26, and Pe: pequi oil only, via gavage. Female offspring were euthanized at 12 months, and ovaries were collected, processed histologically, and sectioned. Histological sections were stained with hematoxylin-eosin to analyze the superficial epithelium height and the tunica albuginea thickness. Immunohistochemistry for AR and ERα was performed, and the percentage of positive nuclei for these receptors was quantified in the theca interna, granulosa cells within follicles, and the interstitial gland. Data were analyzed using analysis of variance (ANOVA), Tukey's, and Kruskal-Wallis tests. The data revealed a significant decrease (p < 0.05) in the thickness of the tunica albuginea in the EE2 group, whereas this thickness was increased in the EE2/Pe and Pe groups. Epithelial height was lower in the EE2 group and higher in the EE2/Pe group. No significant changes in AR immunoreactivity were observed. In multilaminar follicles, ERα immunostaining was elevated in granulosa cells in the Pe group and in theca cells of the EE2 group. Additionally, the interstitial gland in the Pe group showed an increase in ERα expression. Pequi oil exposure upregulated ERα more markedly than AR during folliculogenesis and in interstitial cells, suggesting endocrine-modulating potential and relevance for ovarian regulation during aging.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"57"},"PeriodicalIF":2.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HIV-associated preeclampsia: evaluation of lymphangiogenesis in placental bed samples. hiv相关的先兆子痫：评估胎盘床样本中的淋巴管生成。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2025-05-22 DOI: 10.1007/s00418-025-02359-4

O A Onyangunga, P Naidoo, J Moodley, T Naicker

{"title":"HIV-associated preeclampsia: evaluation of lymphangiogenesis in placental bed samples.","authors":"O A Onyangunga, P Naidoo, J Moodley, T Naicker","doi":"10.1007/s00418-025-02359-4","DOIUrl":"10.1007/s00418-025-02359-4","url":null,"abstract":"The role of angiogenesis in preeclampsia pathogenesis is widely studied; however, despite the lymphatic vessels' complementary role to the blood vascular system, studies on their morphology in the placenta and placental bed are lacking. In total, 87 placental bed specimens were utilized, which were grouped into normotensive pregnant (n = 28), early-onset preeclampsia (n = 31), and late-onset preeclampsia (n = 28), and further stratified by human immunodeficiency virus (HIV) status. Tissue was immunostained with podoplanin antibody to investigate whether HIV infection affects lymphangiogenesis. The lymphatic capillary density and luminal areas within the placental bed were morphometrically assessed. Lymphatic microvessel density and mean area/lumen in the preeclampsia group were higher and larger than in the normotensive group, respectively (p = 0.01 and p = 0.001). A correlation between blood pressure levels and lymphatic microvessel density was observed (r ≥ 0.272; p ≤ 0.032). Significant differences were observed between the mean microvessel density of normotensive HIV-uninfected and HIV-infected groups (5.9 ± 2.3 versus 7.5 ± 2.8, p = 0.01) and late-onset preeclampsia HIV-uninfected and HIV-infected groups (7.1 ± 3.9 versus 7.8 ± 2.7, p = 0.01). The mean area/lumen between normotensive HIV-uninfected and HIV-infected, and late-onset preeclampsia HIV-uninfected and HIV-infected groups were significantly different (p = 0.03 and p = 0.001). Small lymphatic capillaries were significantly abundant in late-onset preeclampsia HIV-infected (p = 0.03) and normotensive HIV-infected (p = 0.0001) groups compared with uninfected groups. Lymphatic capillary density and area/lumen upregulation was observed in the placental bed of HIV-infected women, with a positive correlation between maternal blood pressure and lymphatic microvessel density, potentially affecting birth weight in the preeclampsia group.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"55"},"PeriodicalIF":2.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subcellular localization of the P2X4 receptor in sensory hair cells of Wistar rat cochlea. Wistar大鼠耳蜗感觉毛细胞P2X4受体的亚细胞定位。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2025-05-20 DOI: 10.1007/s00418-025-02386-1

Ziyin Huang, Jacqueline M Ross, Shelly C Y Lin, Prakansha N Kumar, Kevin Roy, Srdjan M Vlajkovic, Peter R Thorne, Haruna Suzuki-Kerr

{"title":"Subcellular localization of the P2X4 receptor in sensory hair cells of Wistar rat cochlea.","authors":"Ziyin Huang, Jacqueline M Ross, Shelly C Y Lin, Prakansha N Kumar, Kevin Roy, Srdjan M Vlajkovic, Peter R Thorne, Haruna Suzuki-Kerr","doi":"10.1007/s00418-025-02386-1","DOIUrl":"10.1007/s00418-025-02386-1","url":null,"abstract":"Our sense of hearing starts in the inner ear organ, the cochlea, which contains two types of auditory hair cells for signal transduction. Earlier research showed that the complex cochlear physiology is regulated in part by purinergic signalling through activation of purine-mediated P2X, P2Y and adenosine receptors expressed in the cochlea. This study aims to extend our knowledge of purinergic signalling in the cochlea by comprehensively characterising the expression of the P2X4 receptor subtype. Wistar rat cochlea (embryonic day 20.5-6 weeks, both sexes) were collected, and the P2X4 expression was examined by immunohistochemistry. Robust P2X4 expression was found in the organ of Corti (OoC) in the inner hair cells (IHCs) and outer hair cells (OHCs), confirmed by double-labelling with hair cells (HCs) marker myosin VIIa. In IHCs, a robust cytoplasmic P2X4 expression occurred throughout the cell body, with the most intense signal at the medial side. In OHCs, P2X4 formed puncta near the apical and basal ends of the cell body. Using markers for subcellular organelles, P2X4 immunoreactivity was predominately associated with the trans-Golgi network apparatus and early endosomes in IHC and with early endosomes and lysosomes in OHC in the mature cochlea. In both cell types, some co-localisation of P2X4 with presynaptic marker was also observed. Taken together, these observations suggest unique roles for P2X4 in mature IHCs and OHCs as a purinergic receptor subtype responsible for the homeostatic regulation of hair cells and auditory sensory transduction.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"54"},"PeriodicalIF":2.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12092529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunocytochemical detection of proteins within cellular structures inaccessible to specific antibodies. 免疫细胞化学检测在特定抗体无法进入的细胞结构内的蛋白质。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2025-05-17 DOI: 10.1007/s00418-025-02387-0

Maria A Tikhomirova, Anastasia A Zharikova, Yana R Musinova, Eugene V Sheval

引用次数: 0

Inhibition of hsa_circ_0003314 contributes to trophoblast cell migration and invasion and inhibits pyroptosis in preeclampsia. 抑制hsa_circ_0003314有助于滋养细胞迁移和侵袭，并抑制子痫前期的焦亡。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2025-05-16 DOI: 10.1007/s00418-025-02384-3

Yun Zhou, Yuqing Chen, Lihua Li, Lizhen Lin

{"title":"Inhibition of hsa_circ_0003314 contributes to trophoblast cell migration and invasion and inhibits pyroptosis in preeclampsia.","authors":"Yun Zhou, Yuqing Chen, Lihua Li, Lizhen Lin","doi":"10.1007/s00418-025-02384-3","DOIUrl":"https://doi.org/10.1007/s00418-025-02384-3","url":null,"abstract":"Inflammation is a key contributor to the development of preeclampsia. Recent studies suggest that circular RNAs (circRNAs) may serve as potential therapeutic targets for this disease, though their specific functions remain incompletely understood. In this study, we investigated the role of hsa_circ_0003314 in preeclampsia pathogenesis. The interaction between hsa_circ_0003314 and microRNA (miR)-1827 was validated using RNA pull-down and luciferase reporter assays, while the binding of miR-1827 to the 3'-UTR of caspase-5 was confirmed by RNA immunoprecipitation and luciferase reporter assays. Pyroptotic cells were quantified by flow cytometry based on the percentage of caspase-1/propidium iodide (PI) double-positive cells. Enzyme-linked immunosorbent assay (ELISA) was performed to measure interleukin (IL)-1β concentrations in the culture supernatant. The migration and invasion abilities of HTR-8/SVneo cells were evaluated using Transwell assays. We found that hsa_circ_0003314 expression was upregulated in HTR-8/SVneo cells subjected to hypoxia/reoxygenation (H/R) treatment. Silencing hsa_circ_0003314 enhanced cell migration, invasion, and epithelial-mesenchymal transition (EMT), while reducing the expression of pyroptosis-related proteins, GSDMD-N and HMGB1. The proportion of pyroptotic cells was significantly decreased upon hsa_circ_0003314 knockdown in H/R-treated cells. Mechanistically, hsa_circ_0003314 functions as a molecular sponge for miR-1827, thereby regulating caspase-5 expression. Notably, caspase-5 overexpression rescued the effects of hsa_circ_0003314 knockdown, restoring pyroptosis markers and suppressing the enhanced migratory and invasive behavior of HTR-8/SVneo cells. In conclusion, silencing hsa_circ_0003314 promotes migration, invasion, and EMT in H/R-treated HTR-8/SVneo cells by inhibiting caspase-5-mediated pyroptosis through the sequestration of miR-1827. These findings identify hsa_circ_0003314 as a promising therapeutic target in the treatment of preeclampsia.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"52"},"PeriodicalIF":2.1,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: The amelioration effects of ankaferd blood stopper, platelet gel, and Momordica charantia on peripheral nerve injury in the rats: a stereological and ultrastructural study. 校正：红血球止血剂、血小板凝胶、苦瓜对大鼠周围神经损伤的改善作用：体视学和超微结构研究。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2025-05-15 DOI: 10.1007/s00418-025-02371-8

Gamze Altun, Mehmet Emin Önger, Stefano Geuna, Abubaker El Elhaj, Stefania Raimondo, Ömür Gülsüm Deniz, Suleyman Kaplan

引用次数: 0