Histochemistry and Cell Biology最新文献

{"title":"The ILK-PINCH-parvin complex: a conserved primary adhesome regulating biological processes.","authors":"Ushashi Ain, Benazir Fatma, Hena Firdaus","doi":"10.1007/s00418-025-02408-y","DOIUrl":"https://doi.org/10.1007/s00418-025-02408-y","url":null,"abstract":"<p><p>Intracellularly expressed integrin associated proteins (IAPs) play pivotal role in facilitating cellular adhesion and survival. The integrin linked kinase (ILK)-PINCH-parvin (IPP) complex regulates cell-matrix interactions crucial for tissue development and homeostasis by functioning as an adapter between integrin and actin cytoskeleton. This review provides a compiled structural and functional insight into the IPP complex and its interacting partners, highlighting its conservation and signalling pathway, addressing a cross-talk across its homologues. Finally, the study sheds light on the association of the complex members with biological processes and disease progression, thus potentiating further exploration of the IPP complex within the integrin signalling pathway.</p>","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"78"},"PeriodicalIF":2.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Excessive intake of fructose and alcohol aggravates high-fat diet-induced steatohepatitis and changes hepatic iron homeostasis.","authors":"Salamah M Alwahsh, Min Xu, Ahmad Itmezeh, Myung-Ho Kim, Giuliano Pasquale Ramadori","doi":"10.1007/s00418-025-02402-4","DOIUrl":"https://doi.org/10.1007/s00418-025-02402-4","url":null,"abstract":"<p><p>Hepatocytes play a vital role in maintaining iron homeostasis, which can be compromised by poor diet and lifestyle. This study aimed to assess the combined impact of high-fat diet (HFD), alcohol, and fructose intake on liver injury and iron metabolism. Male Sprague-Dawley rats (5‒7 weeks old, weighing 160‒180 g) were randomly assigned to three groups: regular chow, HFD, and a combined HFD with 30% ethanol and 30% fructose (HF‒EFr), and were maintained for 4 or 8 weeks. Liver injury was assessed via histopathological staining and serum aminotransferase levels. Iron homeostasis was evaluated by measuring plasma iron levels, hepatic iron deposition, and the expression of key iron-regulatory proteins. Markers of inflammation, oxidative stress, and macrophage activation, such as CD14, ED-1 (CD68), HIF1α, and STAT3 activation, were also examined. Urinary levels of ferritin-L and lipocalin-2 were measured. The HF‒EFr group exhibited severe liver injury, characterized by steatohepatitis and hepatocyte ballooning, with significantly elevated serum aminotransaminase levels and a decrease in the AST/ALT ratio from 3:1 at week 4 to 2:1 at week 8, indicative of progressive hepatocellular damage. Increased serum CD14 and hepatic ED-1 (CD68) confirmed macrophage activation. Urinary ferritin-L and lipocalin-2 levels increased significantly compared with other groups, indicating systemic inflammation and renal involvement. In the HF‒EFr group, gene expression of ferroportin and ferritin was upregulated, accompanied by plasma iron depletion and increased hepatic iron deposition. Nuclear localization of ferritin subunits in hepatocytes was observed, along with elevated HIF-1α and cytokine levels, and activation of STAT3, suggesting an oxidative stress response in the HF‒EFr group. Chronic consumption of alcohol and fructose intensifies the dysregulation of iron metabolism and liver injury induced by a high-fat diet. These findings highlight the critical role of iron homeostasis in diet-induced liver disease and suggest that urinary biomarkers such as ferritin-L and lipocalin-2 may serve as noninvasive indicators of systemic inflammation and iron dysregulation. This study underscores the need for early detection strategies and long-term dietary interventions to limit metabolic liver disease progression.</p>","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"77"},"PeriodicalIF":2.1,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differentiation to insulin-positive cells from human amnion epithelial cells using a pancreatic development mimicry protocol.","authors":"Daniel Martínez-Rodríguez, Jonathan Salazar-Alonso, Axel Castro-Abrego, Daniela Ávila-González, Omar Martínez-Alarcón, Anayansi Molina-Hernández, Alejandro Martínez-Juárez, Héctor Salvador Godoy-Morales, Diana Elizabeth Lara-Barragán, Wendy Portillo, Nestor Emmanuel Díaz-Martínez, Guadalupe García-López, Nestor Fabián Díaz","doi":"10.1007/s00418-025-02400-6","DOIUrl":"10.1007/s00418-025-02400-6","url":null,"abstract":"<p><p>Diabetes mellitus (DM) is characterized by the loss or dysfunction pancreatic β-cells. Human amniotic epithelial cells (hAEC), which retain pluripotency markers and are readily obtainable from term placentas, represent a promising alternative source of stem cells. We investigated whether hAECs can be guided through pancreatic ontogeny to generate insulin-producing β-like cells. hAEC from uncomplicated term deliveries were expanded to passage 1 and exposed to a four-stage differentiation sequence that sequentially modulated Activin/WNT, KGF/TGF-β, retinoic-acid/hedgehog, and EGF/Noggin signaling. Stage progression was monitored by end-point RT-PCR and quantitative immunofluorescence for hallmark transcription factors. After definitive endoderm induction, 64% of cells were Brachyury positive and 71% were WNT3A positive; primitive-gut specification yielded 57% HNF1B⁺ cells. Posterior foregut commitment produced 75% PDX1⁺ and 60% Sox9⁺ nuclei and the final endocrine stage generated 74% NKX2.2⁺ cells, with 71% showing cytoplasmatic insulin and 51% C-peptide staining; insulin/C-peptide co-localization was confirmed by double labelling. Thus a chemically defined, four-step protocol can convert term-derived hAEC into insulin-producing β-like cells, supporting their use as an accessible platform for diabetes modelling and future cell-replacement therapies.</p>","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"76"},"PeriodicalIF":2.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12259741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin alleviates ovarian hyperstimulation syndrome in the rat model via inhibiting the nuclear factor kappa B/hypoxia-inducible factor-1α signaling pathway.","authors":"Lilan Sun, Xiumei Zhuo, Dandan Qi, Hao Ma, Fanhua Kong, Pingping Sun, Yan Zhang, Peng Xue, Huagang Ma","doi":"10.1007/s00418-025-02403-3","DOIUrl":"https://doi.org/10.1007/s00418-025-02403-3","url":null,"abstract":"<p><p>Ovarian hyperstimulation syndrome (OHSS) is the most severe complication of ovarian stimulation during assisted reproductive procedures and resembles an acute inflammatory response. Curcumin possesses antioxidant, anti-inflammatory, and anti-angiogenic properties. However, its impact on OHSS remains unclear. This study aimed to investigate the protective effects of curcumin in a rat model of OHSS and elucidate its underlying mechanisms. A rat model of OHSS was established using gonadotropin and human chorionic gonadotropin (hCG), followed by 7 days of curcumin administration. Pathological changes in ovarian tissue were assessed via hematoxylin and eosin (H&E) staining. The expression levels of serum estradiol (E₂), vascular endothelial growth factor (VEGF), VEGF-receptor 2 (VEGFR2), and inflammatory factors were measured using enzyme-linked immunosorbent assay (ELISA). Additionally, malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) levels were assessed using commercial kits. Finally, nuclear factor kappa B (NF-κB) and hypoxia-inducible factor 1-alpha (HIF-1α) levels in ovarian tissue were analyzed using western blotting. The results showed that curcumin significantly alleviated ovarian tissue pathological damage in rats with OHSS and reduced the serum levels of E₂, VEGF, VEGFR2, interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α). Additionally, SOD and GSH-Px levels were significantly elevated, while MDA levels were significantly diminished. Moreover, curcumin significantly inhibited NF-κB activation, downregulated HIF-1α expression, and consequently reduced VEGF expression. In summary, curcumin reduced VEGF levels by inhibiting the NF-κB/HIF-1α signaling pathway, thereby alleviating symptoms of gonadotropin-induced OHSS. It may serve as a potential therapeutic agent for OHSS prevention and treatment.</p>","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"75"},"PeriodicalIF":2.1,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144617393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"June in focus in HCB.","authors":"Douglas J Taatjes, Jürgen Roth","doi":"10.1007/s00418-025-02401-5","DOIUrl":"https://doi.org/10.1007/s00418-025-02401-5","url":null,"abstract":"","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"74"},"PeriodicalIF":2.1,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144617394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uridine and uridine nucleotides as novel regulators of NUCB2/nesfatin-1 neuron activation: an immunohistochemical study.","authors":"N Hasanoğlu Akbulut, G Topal, C Koç, N Coşkan, B Salman, M Cansev, O Eyigor","doi":"10.1007/s00418-025-02404-2","DOIUrl":"https://doi.org/10.1007/s00418-025-02404-2","url":null,"abstract":"<p><p>Nesfatin-1, identified as an anorexigenic neuropeptide in the hypothalamus, is activated by glutamatergic agonists and innervated by excitatory and inhibitory neurons. The uridine nucleotide uridine diphosphate was recently identified as a novel regulator of feeding-related neurons. However, the activating effects of uridine and uridine nucleotides on anorexigenic NUCB2/nesfatin-1 neurons are unknown. In this study, the activating effects of intracerebroventricularly administered uridine or uridine nucleotides (UMP, UDP, UTP) on NUCB2/nesfatin-1 neurons localized in the supraoptic (SON), paraventricular (PVN), and periventricular (PeV) nuclei were determined using c-Fos immunohistochemistry. Results were evaluated as the percentage of the ratio of c-Fos-expressing (activated) NUCB2/nesfatin-1 neurons to all NUCB2/nesfatin-1 neurons. It was determined that centrally administered uridine, UMP, and UTP activated a statistically significant number of NUCB2/nesfatin-1 neurons in the SON, PVN, and PeV, compared with the saline group (p < 0.05). At the same time, the slight increase in the neuronal activation seen following UDP application was not found to be significant. The results of this study show that NUCB2/nesfatin-1 neurons respond to uridine and uridine nucleotides in the form of neuronal activation, possibly through pyrimidinergic neurotransmission.</p>","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"73"},"PeriodicalIF":2.1,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144583705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The protective role of vitamin E against the effects of hyperthyroidism on the rat pituitary-ovary axis.","authors":"Elfide Gizem Bakırhan, Süleyman Kaplan","doi":"10.1007/s00418-025-02399-w","DOIUrl":"10.1007/s00418-025-02399-w","url":null,"abstract":"<p><p>This study investigated the effects of an experimentally induced hyperthyroidism model on the pituitary-ovarian axis and the possible protective role of vitamin E against these adverse effects. Wistar albino rats were divided into five groups of eight animals each: control (\"Cont,\" no any treatment); hyperthyroidism (\"Hypert,\" L-thyroxine at 0.3 mg/kg/day); \"Sham\" (1 ml/day corn oil); \"Hypert + vit E\" (L-thyroxine (0.3 mg/kg/day and 100 mg/kg/day/1 ml vitamin E); and \"Vit E\" (100 mg/kg/day/1 ml vitamin E) groups. At the end of the experiment, ovarian tissues were exposed to electron microscopic and stereological analyses. Thyroid stimulating hormone (TSH), free thyroxine (FT4), follicle stimulating hormone (FSH), luteinizing hormone (LH), and superoxide dismutase (SOD) levels, as well as catalase (CAT) enzyme activity, were also determined in blood serum samples. Ovarian follicle numbers and volumes; corpus luteum and cortex volume; body weights; and TSH, FSH, and SOD levels decreased significantly in the Hypert group compared with the Cont group. However, connective tissue volume, CAT enzyme activity, and FT4 levels increased in the Hypert group compared with the Cont group. Vitamin E supplementation was observed to play a protective role on antral follicle, corpus luteum, and connective tissue volumes, CAT activity, and SOD and FSH levels. Hyperthyroidism reduces the number of ovarian follicles and may cause infertility problems. The adverse effect of hyperthyroidism on the pituitary-ovarian axis can be ameliorated by means of vitamin E, since this may have a homeostatic effect on this axis, as shown by the biochemical, histopathological, and stereological analyses in this study.</p>","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"72"},"PeriodicalIF":2.1,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12226660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of endocannabinoid system receptors in the equine hoof: dysregulation as a potential therapeutic target for laminitis.","authors":"Rodrigo Zamith Cunha, Francesca Gobbo, Maria Morini, Augusta Zannoni, Carlo Mainardi, Lorenzo D'arpe, Alessandro Gramenzi, Roberto Chiocchetti","doi":"10.1007/s00418-025-02397-y","DOIUrl":"https://doi.org/10.1007/s00418-025-02397-y","url":null,"abstract":"<p><p>A growing body of evidence indicates that the endocannabinoid system (ECS) is essential for controlling the homeostasis of the skin and that the ECS is modified in the presence of skin disease. It is plausible to expect that the lamellar junction of the hoof expresses cannabinoid receptors and that their expression could be affected by lamellar disease. The goal of this study was to characterise the cannabinoid receptor type 1 (CB1R) and type 2 (CB2R) and the G protein-coupled receptor 55 (GPR55) within the dermo-epidermal junction of the hooves of healthy and laminitic horses. The expression of the CB1R, CB2R, and GPR55 within the dermo-epidermal lamellar junction of six healthy and 12 laminitic hooves was studied using polymerase chain reaction (PCR) and immunofluorescence. Both the mRNA and protein expression of the CB1R, CB2R, and GPR55 were found in the dermo-epidermal lamellar junction of horse hooves. The immunolabelling was expressed by the epithelial cells of the primary and secondary laminae of healthy hooves (CB2R > GPR55 > CB1R). The presence of CB1R, CB2R, and GPR55 immunoreactivity in the healthy laminar epithelial cells, coupled with increased protein expression in pathological epithelial cells, provided strong motivation for future investigation. These findings suggest that cannabinoid compounds which interact with these receptors may influence lamellar healing and mitigate inflammation in hoof diseases, particularly laminitis.</p>","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"71"},"PeriodicalIF":2.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review of histone modifications during mammalian oogenesis and early embryo development.","authors":"Nazlican Bozdemir, Tuba Kablan, Efe Biyikli, Ozgur Cinar, Fatma Uysal","doi":"10.1007/s00418-025-02398-x","DOIUrl":"10.1007/s00418-025-02398-x","url":null,"abstract":"<p><p>The success of both oogenesis and early embryo development relies heavily on dynamic epigenetic regulation in which gene activity changes without affecting the underlying DNA sequence. Epigenetics works through two main mechanisms: DNA methylation and histone modifications. DNA methylation typically leads to gene silencing, while histone modifications can either activate or repress genes depending on the specific modification, histone type, and targeted amino acid residue. Histone modifications affect important DNA regulatory processes in which the histone core area as well as the N-terminal tails that extend from the core region are vulnerable to a variety of posttranslational modifications (PTMs), including methylation, citrullination (deimination), acetylation, phosphorylation, ubiquitination, SUMOylation, ribosylation, and lactylation. This review article focuses on what is known about changes in the histone modifications and how these modifications and their responsible enzymes operate throughout mammalian oocyte maturation and early embryo development, highlighting their crucial roles in these processes.</p>","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"70"},"PeriodicalIF":2.1,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12206194/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Citric acid as a safe alternative to oxalic acid in the Gomori reticulin technique: a comparative study.","authors":"Ana Bento, Ana Nascimento, Sofia Nobre, Teresa Ferreira, Amadeu Borges-Ferro, Ana Marques-Ramos","doi":"10.1007/s00418-025-02392-3","DOIUrl":"10.1007/s00418-025-02392-3","url":null,"abstract":"<p><p>The use of oxalic acid is common in metallic impregnation techniques, where it serves as a reducing and bleaching agent for potassium permanganate. Given that oxalic acid is harmful to health, its replacement with another reagent is advantageous for healthcare professionals handling it in laboratory settings. Considering that citric acid can also reduce potassium permanganate, the aim of this study was to determine whether oxalic acid in the Gomori reticulin technique could be replaced by citric acid, a compound substantially less hazardous to health. To this end, 1%, 5%, and 10% citric acid solutions were tested on porcine liver and kidney samples, and the intensity of reticulin fiber staining, contrast, and overall morphological preservation were assessed in comparison with 1% oxalic acid. The results showed no statistically significant differences between the positive control (oxalic acid) and the citric acid protocols, with the 5% citric acid concentration proving most favorable in terms of required incubation time and the evaluated morphological parameters. It was therefore concluded that citric acid can be used as a bleaching agent for potassium permanganate in the Gomori technique, effectively replacing oxalic acid.</p>","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"69"},"PeriodicalIF":2.1,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187824/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144474994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}