Histochemistry and Cell Biology最新文献

Multimodal tumor suppression by METTL3 gene knockdown in melanoma and colon cancer cells.

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-12-25 DOI: 10.1007/s00418-024-02346-1

Arezoo Bazargani, Masoumeh Fakhr Taha, Bahram Mohammad Soltani, Arash Javeri

{"title":"Multimodal tumor suppression by METTL3 gene knockdown in melanoma and colon cancer cells.","authors":"Arezoo Bazargani, Masoumeh Fakhr Taha, Bahram Mohammad Soltani, Arash Javeri","doi":"10.1007/s00418-024-02346-1","DOIUrl":"https://doi.org/10.1007/s00418-024-02346-1","url":null,"abstract":"METTL3, an m6A methyltransferase, is integral to the regulation of messenger RNA (mRNA) biogenesis, degradation, and translation through the N6-methyladenosine (m6A) modification. Alterations in m6A homeostasis have been implicated in the development, progression, invasion, and metastasis of certain cancers. The present research aims to examine the consequences of METTL3 knockdown using short hairpin RNA (shRNA) on the proliferation and invasive capabilities of human colorectal and melanoma cancer cell lines. A specific shRNA against METTL3 mRNA was designed and inserted into an expression vector. Highly invasive colorectal cancer cell line SW480 and melanoma cell line A375 were cultured and transfected by METTL3-shRNA and scramble-control vectors and kept under culture condition for 2 weeks. The cells were harvested for analysis of gene expression by quantitative polymerase chain reaction (qPCR), invasion assay using three-dimensional (3D) spheroid assay and cell cycle and apoptosis analyses. In the METTL3-shRNA transfected cells, the expression of METTL3, VIM, SNAI1, SNAI2, ZEB1, CDH1, and TGFB1 genes were downregulated significantly compared with the scramble-control transfected cells. Expression of b-catenin, N-cadherin, vimentin, ZEB1, pro- and active MMP2, OCT4A, SOX2, and MYC proteins were also downregulated following METTL3 knockdown. Transfection by METTL3-shRNA reduced proliferation rate of the cells and increased the apoptotic rate significantly. Both migration and invasion rate of the cancer cells transfected with METTL3-shRNA were significantly decreased. These findings highlight the pro-oncogenic function of METTL3 in colorectal and melanoma cancer cells, indicating that inhibiting METTL3 could be a promising approach for tumor suppression across multiple cancer types; nonetheless, further investigation is essential to confirm these observations.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"21"},"PeriodicalIF":2.1,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ku70 silencing aggravates oxygen-glucose deprivation/reperfusion-induced injury by activation of the p53 apoptotic pathway in rat cortical astrocytes.

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-12-23 DOI: 10.1007/s00418-024-02352-3

Xiaoyun Xie, Jingli Liu

{"title":"Ku70 silencing aggravates oxygen-glucose deprivation/reperfusion-induced injury by activation of the p53 apoptotic pathway in rat cortical astrocytes.","authors":"Xiaoyun Xie, Jingli Liu","doi":"10.1007/s00418-024-02352-3","DOIUrl":"https://doi.org/10.1007/s00418-024-02352-3","url":null,"abstract":"Oxidative stress-induced DNA damage is an important mechanism that leads to the death of neuronal cells after ischemic stroke. Our previous study found that Ku70 was highly expressed in ischemic brain tissue of rats after cerebral ischemia-reperfusion injury. However, the role of Ku70 in glucose-oxygen deprivation/reperfusion (OGD/R) in astrocytes has not been reported. Therefore, we investigated the effect and mechanism of Ku70 on OGD/R-induced astrocyte injury in rats. Rat astrocytes were cultured in vitro to establish the OGD/R-induced injury model and transfected with small interfering RNA (siRNA) to disturb Ku70 expression. Real-time quantitative polymerase chain reaction (RT-qPCR), western blotting, and immunofluorescence were performed to assay the expression of mRNA and proteins. Cell viability, apoptosis, and ROS accumulation were determined by CCK-8 assay, flow cytometry, and fluorescence microscopy, respectively. Our results showed Ku70 can be expressed in both the nucleus and cytoplasm of astrocytes, although mainly in the nucleus. Ku70 expression showed a trend of first increasing and then decreasing after OGD/R, reaching its highest change at 24 h of reoxygenation. OGD/R induced ROS production and DNA damage in rat astrocytes, and Ku70 silencing further increased ROS production and DNA lesions, which aggravated astrocyte injury and apoptosis. Furthermore, the expression of p53, Bax, and caspase 3 proteins significantly increased after OGD/R in astrocytes, and downregulation of Ku70 further enhanced expression of the above proteins. These results indicate that Ku70 silencing promotes OGD/R-induced astrocyte apoptosis, which may be associated with p53 apoptotic pathway activation. Our study suggests that Ku70 may be a novel target for cerebral ischemia-reperfusion injury therapy.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"20"},"PeriodicalIF":2.1,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of bone marrow niche on hematopoietic stem cells.

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-12-23 DOI: 10.1007/s00418-024-02348-z

Vahid Niazi, Soudeh Ghafouri-Fard

引用次数: 0

Protective effects of adipose-derived stem cells against testicular injury induced after ischemia-reperfusion by regulating autophagy.

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-12-21 DOI: 10.1007/s00418-024-02347-0

Ebru Alimogullari, Bahar Kartal, Hazal Demir, Mualla Pınar Elci

{"title":"Protective effects of adipose-derived stem cells against testicular injury induced after ischemia-reperfusion by regulating autophagy.","authors":"Ebru Alimogullari, Bahar Kartal, Hazal Demir, Mualla Pınar Elci","doi":"10.1007/s00418-024-02347-0","DOIUrl":"https://doi.org/10.1007/s00418-024-02347-0","url":null,"abstract":"The damaged organ may experience severe pathological alterations as a result of tissue ischemia-reperfusion (I/R). The study of stem cell-based repair therapies is actively being conducted, and the outcomes and therapeutic potential of these cells are both promising. Autophagy checks protein homeostasis by breaking down huge damaged proteins or organelles. The study's objective was to assess how ADSCs impact the autophagic process after testicular ischemia/reperfusion. In our investigation, 30 male rats were divided into five groups: control, ADSC, ischemia, I/R, and I/R + ADSC (n = 6). Hematoxylin-eosin (HE) was used to stain the testes, and histological changes were assessed. The immunoexpression of androgen receptor (AR), Beclin1, protein light chain 3B (LC3B), and p62 were examined. The seminiferous epithelium in the testis from the ischemia and I/R groups revealed significant degeneration with disorganized morphology, damaged spermatogenic cells, and interstitial space congestion, according to HE stain results. Johnsen's score were significantly better in I/R + ADSC group than in ischemia and I/R groups. We demonstrated that in rat testes from the I/R groups, immunostaining of Beclin 1 (p = 0.042) and LC3B (p = 0.011) were raised, and p62 (p = 0.047) and AR (p = 0.049) were decreased. Ischemia and I/R promoted testicular autophagy, therefore we can conclude that ADSCs prevent excessive autophagy. Additionally, these results show that the use of ADSCs cures testicular injury and dysfunction associated with I/R injury in rats even a little.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"18"},"PeriodicalIF":2.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of middle-age plasma therapy on ileum morphology, immune defense (IgA) and cell proliferation (Ki-67) of female aged rats.

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-12-17 DOI: 10.1007/s00418-024-02344-3

Ender Deniz Asmaz, Hikmet Taner Teker, Zeynep Tuğçe Sertkaya, Taha Ceylani, Aysun İnan Genç

{"title":"Effect of middle-age plasma therapy on ileum morphology, immune defense (IgA) and cell proliferation (Ki-67) of female aged rats.","authors":"Ender Deniz Asmaz, Hikmet Taner Teker, Zeynep Tuğçe Sertkaya, Taha Ceylani, Aysun İnan Genç","doi":"10.1007/s00418-024-02344-3","DOIUrl":"https://doi.org/10.1007/s00418-024-02344-3","url":null,"abstract":"Abstarct: Blood plasma therapy, a new treatment method to eliminate the damage and deterioration caused by aging in many organ systems, has attracted increasing attention. The digestive tract, which cooperates with many different systems, has strong effects on our health. In the present study, the effects of plasma therapy on the ileum of elderly rats were investigated. Wistar rats (n = 7; 12-15 months old) were given pooled plasma collected from middle-age rats (6 months, n =28) (for 30 days, 0.3 ml daily, intravenously into the tail vein). At the end of the experiment, villus height, crypt depth, total mucosal thickness and surface absorption area were evaluated. In addition, the effects of IgA, which plays a role in the digestive system's defense against microorganisms, were examined. Both the cell proliferation intensity and proliferation index were evaluated in crypt cells. An increase was determined in all morphological parameters in the experimental group. Similarly, plasma application decreased IgA expression and numbers in the experimental groups. Contrarily, cell proliferation parameters showed a significant increase in the experimental groups' crypt cells. Therefore, we found that the treatment supports the digestive system in terms of both nutrient utilization and absorption-related parameters and has a protective effect on intestinal immune system parameters.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"17"},"PeriodicalIF":2.1,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142835528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-culture of postnatal mouse spinal cord and skeletal muscle explants as an experimental model of neuromuscular interactions.

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-12-02 DOI: 10.1007/s00418-024-02343-4

Mariya M Mikhailova, Olga I Klein, Timofey D Patsaev, Andrey A Panteleyev

{"title":"Co-culture of postnatal mouse spinal cord and skeletal muscle explants as an experimental model of neuromuscular interactions.","authors":"Mariya M Mikhailova, Olga I Klein, Timofey D Patsaev, Andrey A Panteleyev","doi":"10.1007/s00418-024-02343-4","DOIUrl":"https://doi.org/10.1007/s00418-024-02343-4","url":null,"abstract":"The intercommunication between nerves and muscles plays an important role in the functioning of our body, and its failure leads to severe neuromuscular disorders such as spinal muscular atrophy and amyotrophic lateral sclerosis. Understanding the cellular and molecular mechanisms underlying nerve-muscle interactions and mediating their mutual influence is an integral part of strategies aimed at curing neuromuscular diseases. Here, we propose a novel ex vivo experimental model for the spinal cord (SC) and skeletal muscle interactions which for the first time utilizes only fully formed (but not yet quite functional) postnatal tissues. The model represents an organotypic co-culture comprising a longitudinal slice of the mouse postnatal SC and an extensor digitorum longus (EDL) muscle explant placed in the \"damage zone\" of transversally dissected longitudinal slice of the SC. Using this model, we have shown that SC tissue stimulates muscle contractions and reduces the area occupied by acetylcholine receptors on muscle surface. In turn, EDL muscles stimulate the growth of SC-derived neurites. Thus, our organotypic model allows one to assess the mutual influence of neurons and muscles in a nearly natural setting which maintains the architecture and cellular composition of intact tissues. Therefore, this model may provide an effective platform for studying molecular and cellular mechanisms linked to defective neuromuscular interactions in associated pathologies.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":"163 1","pages":"15"},"PeriodicalIF":2.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142768496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel tape-free sample preparation method for human osteochondral cryosections for high throughput hyperspectral imaging.

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-12-02 DOI: 10.1007/s00418-024-02338-1

Xiwei Fan, Bogdan Donose, Michael W M Jones, Daryl Howard, Jari Torniainen, Karl Bertling, Xiao Guo, Cameron M Kewish, Kah Meng Lee, Antonia Rujia Sun, Aleksandar Rakic, Ross Crawford, Isaac O Afara, Indira Prasadam

引用次数: 0

FOXM1 requires IDH1 for late genes expression in mitotic cells. FOXM1 需要 IDH1 才能在有丝分裂细胞中表达晚期基因。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-12-01 Epub Date: 2024-07-22 DOI: 10.1007/s00418-024-02307-8

Balabhaskararao Kancharana, Hashnu Dutta, Nishant Jain

引用次数: 0

Expression of mRNA for molecules that regulate angiogenesis, endothelial cell survival, and vascular permeability is altered in endothelial cells isolated from db/db mouse hearts. 在分离自 db/db 小鼠心脏的内皮细胞中，调节血管生成、内皮细胞存活和血管通透性的分子 mRNA 的表达发生了改变。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-12-01 Epub Date: 2024-09-24 DOI: 10.1007/s00418-024-02327-4

Krzysztof Bartkowiak, Mateusz Bartkowiak, Ewa Jankowska-Steifer, Anna Ratajska, Elżbieta Czarnowska, Marek Kujawa, Olga Aniołek, Justyna Niderla-Bielińska

{"title":"Expression of mRNA for molecules that regulate angiogenesis, endothelial cell survival, and vascular permeability is altered in endothelial cells isolated from db/db mouse hearts.","authors":"Krzysztof Bartkowiak, Mateusz Bartkowiak, Ewa Jankowska-Steifer, Anna Ratajska, Elżbieta Czarnowska, Marek Kujawa, Olga Aniołek, Justyna Niderla-Bielińska","doi":"10.1007/s00418-024-02327-4","DOIUrl":"10.1007/s00418-024-02327-4","url":null,"abstract":"Metabolic syndrome (MetS) is a condition that includes symptoms, such as obesity, hyperglycemia, and hypertension, which elevate cardiovascular risk. An impaired angiogenic response of endothelial cells (ECs) in heart and peripheral organs has been proposed in MetS, but the mechanisms of this phenomenon have not been thoroughly explored. Results obtained from evaluating the whole myocardium are inconsistent, since different types of cells react differently to MetS environment and a variety of molecular pathways are involved in the angiogenic response. Therefore, the aim of this paper was to study one selected pathway-the VEGF/VEGFR pathway, which regulates the angiogenic response and microvascular permeability in ECs isolated from db/db mouse hearts. The expression of mRNAs for VEGF/VEGFR axis proteins was assessed with RT-PCR in ECs isolated from control and db/db mouse myocardium. The density of CD31-, VEGFR2-, and VE-cadherin-positive cells was examined with confocal microscopy, and the ultrastructure of ECs was analyzed with transmission electron microscopy. The aortic ring assay was used to assess the capacity of ECs to respond to angiogenic stimuli. Our results showed a decreased number of microvessels, diminished expression of VE-cadherin and VEGFR2 and widened gaps between the ECs of microcapillaries. The aortic ring assay showed a diminished number of sprouts in db/db mice. These results may indicate that ECs in MetS enhance the production of mRNA for VEGF/VRGFR axis proteins, yet sprout formation and vascular barrier maintenance are limited. These novel data may provide a foundation for further studies on ECs dysfunction in MetS.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":" ","pages":"523-539"},"PeriodicalIF":2.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11455669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142345759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MEIKIN expression and its C-terminal phosphorylation by PLK1 is closely related the metaphase-anaphase transition by affecting cyclin B1 and Securin stabilization in meiotic oocyte. 通过影响减数分裂卵母细胞中细胞周期蛋白B1和Securin的稳定，MEIKIN的表达及其C端被PLK1磷酸化与分裂期-无丝分裂过渡期密切相关。

IF 2.1 4区生物学

Histochemistry and Cell Biology Pub Date : 2024-12-01 Epub Date: 2024-08-02 DOI: 10.1007/s00418-024-02316-7

Li-Hua Fan, Shu-Tao Qi, Zhen-Bo Wang, Ying-Chun Ouyang, Wen-Long Lei, Yue Wang, Ang Li, Feng Wang, Jian Li, Li Li, Yuan-Yuan Li, Yi Hou, Heide Schatten, Wei-Hua Wang, Qing-Yuan Sun, Xiang-Hong Ou

{"title":"MEIKIN expression and its C-terminal phosphorylation by PLK1 is closely related the metaphase-anaphase transition by affecting cyclin B1 and Securin stabilization in meiotic oocyte.","authors":"Li-Hua Fan, Shu-Tao Qi, Zhen-Bo Wang, Ying-Chun Ouyang, Wen-Long Lei, Yue Wang, Ang Li, Feng Wang, Jian Li, Li Li, Yuan-Yuan Li, Yi Hou, Heide Schatten, Wei-Hua Wang, Qing-Yuan Sun, Xiang-Hong Ou","doi":"10.1007/s00418-024-02316-7","DOIUrl":"10.1007/s00418-024-02316-7","url":null,"abstract":"Oocyte meiotic maturation failure and chromosome abnormality is one of the main causes of infertility, abortion, and diseases. The mono-orientation of sister chromatids during the first meiosis is important for ensuring accurate chromosome segregation in oocytes. MEIKIN is a germ cell-specific protein that can regulate the mono-orientation of sister chromatids and the protection of the centromeric cohesin complex during meiosis I. Here we found that MEIKIN is a maternal protein that was highly expressed in mouse oocytes before the metaphase I (MI) stage, but became degraded by the MII stage and dramatically reduced after fertilization. Strikingly, MEIKIN underwent phosphorylation modification after germinal vesicle breakdown (GVBD), indicating its possible function in subsequent cellular event regulation. We further showed that MEIKIN phosphorylation was mediated by PLK1 at its carboxyl terminal region and its C-terminus was its key functional domain. To clarify the biological significance of meikin degradation during later stages of oocyte maturation, exogenous expression of MEIKIN was employed, which showed that suppression of MEIKIN degradation resulted in chromosome misalignment, cyclin B1 and Securin degradation failure, and MI arrest through a spindle assembly checkpoint (SAC)-independent mechanism. Exogenous expression of MEIKIN also inhibited metaphase II (MII) exit and early embryo development. These results indicate that proper MEIKIN expression level and its C-terminal phosphorylation by PLK1 are critical for regulating the metaphase-anaphase transition in meiotic oocyte. The findings of this study are important for understanding the regulation of chromosome segregation and the prevention meiotic abnormality.","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":" ","pages":"447-464"},"PeriodicalIF":2.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141874677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0