抗炎和神经胶质反应可维持三甲基锡处理过的大鼠的正常结肠功能。

IF 2.1 4区 生物学 Q4 CELL BIOLOGY
Histochemistry and Cell Biology Pub Date : 2024-12-01 Epub Date: 2024-08-22 DOI:10.1007/s00418-024-02320-x
Dian Eurike Septyaningtrias, Nur Salisa Siddik Muliyantoro, Yustina Andwi Ari Sumiwi, Rina Susilowati
{"title":"抗炎和神经胶质反应可维持三甲基锡处理过的大鼠的正常结肠功能。","authors":"Dian Eurike Septyaningtrias, Nur Salisa Siddik Muliyantoro, Yustina Andwi Ari Sumiwi, Rina Susilowati","doi":"10.1007/s00418-024-02320-x","DOIUrl":null,"url":null,"abstract":"<p><p>Studies on the contribution of enteric neuropathy and intestinal homeostasis to central nervous system degeneration using animal models have reported varying results. Recently, colonic myenteric plexus degeneration was observed in trimethyltin-treated rats. Further characterization of this animal model is necessary to determine its potential for investigating the relationship between the enteric nervous system and central nervous system degeneration. In this study, trimethyltin-treated rats (8 mg/kg body weight, i.p.) were used to measure colonic function, structure, and possible colon abnormalities. The colonic function was assessed by measuring fecal pellet output and transit time. Hematoxylin and eosin staining and immunohistochemistry were performed to evaluate inflammatory profiles and intestinal epithelial cell homeostasis. The expression of mRNA encoding tight junction proteins was quantified with quantitative PCR to determine colon permeability. Histological examination of the colon revealed mucosal immune cell infiltration, crypt damage, and high iNOS and arginase-1 expression in the mucosal layer of trimethyltin-treated rats. At the same time, trimethyltin induced high expression of iNOS, arginase-1, and GFAP and increased cell death in the colonic myenteric plexus. The low cell proliferation and low goblet cell distribution suggested altered intestinal epithelial cell homeostasis in trimethyltin-treated rats. Trimethyltin also upregulated claudin 1 expression. However, normal colon function was preserved. In conclusion, the results show that trimethyltin induces colon inflammation and cell death in the colonic myenteric plexus, and disrupts intestinal epithelial cell homeostasis. However, the balance between anti-inflammatory and pro-inflammatory responses maintains normal colon function in trimethyltin-treated rats.</p>","PeriodicalId":13107,"journal":{"name":"Histochemistry and Cell Biology","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Anti-inflammatory and glial response maintain normal colon function in trimethyltin-treated rats.\",\"authors\":\"Dian Eurike Septyaningtrias, Nur Salisa Siddik Muliyantoro, Yustina Andwi Ari Sumiwi, Rina Susilowati\",\"doi\":\"10.1007/s00418-024-02320-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Studies on the contribution of enteric neuropathy and intestinal homeostasis to central nervous system degeneration using animal models have reported varying results. Recently, colonic myenteric plexus degeneration was observed in trimethyltin-treated rats. Further characterization of this animal model is necessary to determine its potential for investigating the relationship between the enteric nervous system and central nervous system degeneration. In this study, trimethyltin-treated rats (8 mg/kg body weight, i.p.) were used to measure colonic function, structure, and possible colon abnormalities. The colonic function was assessed by measuring fecal pellet output and transit time. Hematoxylin and eosin staining and immunohistochemistry were performed to evaluate inflammatory profiles and intestinal epithelial cell homeostasis. The expression of mRNA encoding tight junction proteins was quantified with quantitative PCR to determine colon permeability. Histological examination of the colon revealed mucosal immune cell infiltration, crypt damage, and high iNOS and arginase-1 expression in the mucosal layer of trimethyltin-treated rats. At the same time, trimethyltin induced high expression of iNOS, arginase-1, and GFAP and increased cell death in the colonic myenteric plexus. The low cell proliferation and low goblet cell distribution suggested altered intestinal epithelial cell homeostasis in trimethyltin-treated rats. Trimethyltin also upregulated claudin 1 expression. However, normal colon function was preserved. In conclusion, the results show that trimethyltin induces colon inflammation and cell death in the colonic myenteric plexus, and disrupts intestinal epithelial cell homeostasis. However, the balance between anti-inflammatory and pro-inflammatory responses maintains normal colon function in trimethyltin-treated rats.</p>\",\"PeriodicalId\":13107,\"journal\":{\"name\":\"Histochemistry and Cell Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Histochemistry and Cell Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00418-024-02320-x\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/8/22 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Histochemistry and Cell Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00418-024-02320-x","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/22 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

利用动物模型对肠道神经病变和肠稳态对中枢神经系统变性的影响进行研究的结果各不相同。最近,在三甲基锡处理的大鼠体内观察到结肠肠肌丛变性。为了确定该动物模型在研究肠道神经系统与中枢神经系统变性之间关系的潜力,有必要对该动物模型进行进一步的特征描述。在本研究中,使用三甲基锡处理的大鼠(8 毫克/千克体重,静脉注射)来测量结肠功能、结构和可能的结肠异常。通过测量粪便排出量和转运时间来评估结肠功能。进行了苏木精和伊红染色以及免疫组化,以评估炎症概况和肠上皮细胞稳态。通过定量 PCR 对编码紧密连接蛋白的 mRNA 的表达进行定量,以确定结肠的通透性。结肠组织学检查显示,三甲基锡处理的大鼠粘膜免疫细胞浸润、隐窝损伤、粘膜层中 iNOS 和精氨酸酶-1 高表达。同时,三甲基锡诱导 iNOS、精氨酸酶-1 和 GFAP 的高表达,并增加结肠肠肌丛的细胞死亡。低细胞增殖率和低鹅口疮细胞分布表明,三甲基锡处理的大鼠肠上皮细胞平衡发生了改变。三甲基锡还会上调 claudin 1 的表达。然而,正常的结肠功能得以保留。总之,研究结果表明,三甲基锡会诱发结肠炎症和结肠肠肌丛细胞死亡,并破坏肠上皮细胞的平衡。然而,抗炎和促炎反应之间的平衡可维持三甲基锡处理大鼠的正常结肠功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Anti-inflammatory and glial response maintain normal colon function in trimethyltin-treated rats.

Anti-inflammatory and glial response maintain normal colon function in trimethyltin-treated rats.

Studies on the contribution of enteric neuropathy and intestinal homeostasis to central nervous system degeneration using animal models have reported varying results. Recently, colonic myenteric plexus degeneration was observed in trimethyltin-treated rats. Further characterization of this animal model is necessary to determine its potential for investigating the relationship between the enteric nervous system and central nervous system degeneration. In this study, trimethyltin-treated rats (8 mg/kg body weight, i.p.) were used to measure colonic function, structure, and possible colon abnormalities. The colonic function was assessed by measuring fecal pellet output and transit time. Hematoxylin and eosin staining and immunohistochemistry were performed to evaluate inflammatory profiles and intestinal epithelial cell homeostasis. The expression of mRNA encoding tight junction proteins was quantified with quantitative PCR to determine colon permeability. Histological examination of the colon revealed mucosal immune cell infiltration, crypt damage, and high iNOS and arginase-1 expression in the mucosal layer of trimethyltin-treated rats. At the same time, trimethyltin induced high expression of iNOS, arginase-1, and GFAP and increased cell death in the colonic myenteric plexus. The low cell proliferation and low goblet cell distribution suggested altered intestinal epithelial cell homeostasis in trimethyltin-treated rats. Trimethyltin also upregulated claudin 1 expression. However, normal colon function was preserved. In conclusion, the results show that trimethyltin induces colon inflammation and cell death in the colonic myenteric plexus, and disrupts intestinal epithelial cell homeostasis. However, the balance between anti-inflammatory and pro-inflammatory responses maintains normal colon function in trimethyltin-treated rats.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Histochemistry and Cell Biology
Histochemistry and Cell Biology 生物-细胞生物学
CiteScore
4.90
自引率
8.70%
发文量
112
审稿时长
1 months
期刊介绍: Histochemistry and Cell Biology is devoted to the field of molecular histology and cell biology, publishing original articles dealing with the localization and identification of molecular components, metabolic activities and cell biological aspects of cells and tissues. Coverage extends to the development, application, and/or evaluation of methods and probes that can be used in the entire area of histochemistry and cell biology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信