Hacettepe Journal of Biology and Chemistry最新文献

{"title":"ENHANCED ANTICANCER POTENCY OF GEMCITABINE IN COMBINATION WITH PROPOFOL IN PROSTATE CANCER","authors":"E. Karataş, K. Bayindirli","doi":"10.15671/HJBC.796377","DOIUrl":"https://doi.org/10.15671/HJBC.796377","url":null,"abstract":"I n the present study, the effects of propofol which is an intravenous anesthetic on its own and simultaneously with gemcitabine were investigated on human prostate cancer brain metastasis DU145 and bone metastasis PC3 cells at both cellular and molecular levels. In the first stage of our study, toxic doses of these agents were determined by using the CVDK-8 and lactate dehydrogenase release test. In the following phases, TAC and TOS analyzes were performed to determine the biochemical effects of these agents on cell lines, and also western blot analysis was used to show the inhibition of important oncogenic PI3K/AKT/mTOR pathway in cells treated with these agents. Propofol was found to increase the effectiveness of gemcitabine in both cells. When propofol and gemcitabine were administered simultaneously at high concentrations, they reduced cell viability and increased LDH activity. According to the results obtained from the western blot analysis, the combination of these two agents was found to lead to synergistic inhibition of the PI3K/Akt/mTOR pathway.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"15 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78173959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of smoke and heat-shock on germination in eight perennial Reseda species (Resedaceae)","authors":"Emre Çilden, Gökhan Ergan, C. Ülgen, Ş. Yildirimli, Ç. Tavşanoğlu","doi":"10.15671/HJBC.768365","DOIUrl":"https://doi.org/10.15671/HJBC.768365","url":null,"abstract":"Reseda is an understudied genus regarding germination ecology. We studied the germination response of eight perennial Reseda species to fire related cues by performing a germination experiment including two aqueous smoke treatments (at 1:5 and 1:20 concentrations), one heat shock (100°C for 5 min.) treatment, and two corresponding controls. Smoke slightly decreased germination percentage in R. aucheri ssp. rotundifolia and R. minoica, and the reduction in R. luteola was statistically significant (P < 0.01). In contrary, smoke promoted germination in R. coodei, R. globulosa, and R. orientalis slightly, and markedly in R. anatolica (from 3 to 11%; P = 0.055). Heat shock treatment did not affect the germination in three of four species tested, but significantly increased germination percentage in R. luteola in comparison to the control (P = 0.046). Consequently, there was no consistent germination response to smoke or heat shock in Reseda species studied. This study is the most comprehensive study tested fire-related germination of several Reseda species and provides the first germination results on seven Reseda species. Further studies that include more species and treatments would show a more completed figure for fire-related germination patterns in the genus Reseda.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"322 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80268074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Faunistic study on the family Meloidae (Coleoptera) of Ankara province (Turkey)","authors":"M. Demir, M. Kabalak","doi":"10.15671/HJBC.788113","DOIUrl":"https://doi.org/10.15671/HJBC.788113","url":null,"abstract":"Fabricius, 1775 is the most species-rich genus with 10 species. Cerocoma (s.str.) bernhaueri Pardo Alcaide, 1977, Alosimus luteus (Waltl, 1838), Teratolytta monticola Bologna, 2006, Hycleus polymorphus (Pallas, 1771), H. sexmaculatus (A.G. Olivier, 1811), Mylabris ( Micrabris ) unicolor Falder -mann, 1837, Meloe ( Eurymeloe ) mediterraneus (G. Muller, 1925) and Stenoria (s.str.) apicalis (Latreille, 1804) species were recorded from Central Anatolian Region of Turkey for the first time. Detailed locality records of M . ( Micrabris ) unicolor and S. (s.str.) apicalis in Turkey were given with this study for the first time. The most western distributions of Alosimus luteus and Teratolytta monticola were given with this study.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83196173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Charge recombination suppressed CdSeS/CdSe/ZnS QDSSC design","authors":"Erdem Eli̇bol","doi":"10.15671/HJBC.795908","DOIUrl":"https://doi.org/10.15671/HJBC.795908","url":null,"abstract":"he interest in quantum sensitized solar cells (QDSSC), which theoretically to up to energy con version is it has theoretically high efficiency value, obtained in studies with QDSSCs is far these This shows that there are many difficulties to be solved in QDSSC technology. One of the main challenges in QDSSC technology is irradiated load recombination occurring in QDSSC. For this reason, in this study, it is about using CdSeS QDs as an alternative to the most used CdS QDs in the literature in order to supp ress the load recombination between TiO 2 surface and electrolyte and QD surfaces. In the study, while CdS and CdSeS QDs were coated on the TiO 2 surface with SILAR method, the previously synthesized CdSe QD was coated with chemical deep deposition method. Surfaces were last treated with ZnS QDs. An optimization study was carried out to determine the ideal number of CdSeS coatings for QDSSCs. As a result, the Jsc and Voc values for TiO 2 /CdSeS 4 /CdSe/ZnS QDSSCs were 8.799 mA/ cm 2 and 0.795 V, respectively, while the PCE value increased to 4.452%.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"2014 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86519995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of various inhibitors on enzymatic browning, antioxidant activity and total phenol content of fresh-cut rocket salad (Eruca sativa Mill.)","authors":"Arzu Altunkaya Dinçay","doi":"10.15671/HJBC.649050","DOIUrl":"https://doi.org/10.15671/HJBC.649050","url":null,"abstract":"antioxidant activity of rocket significantly, Rocket phenolics were protected from oxidation by the treatments of these inhibitors.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90075527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating Usage Potential of Datura stramonium L. for Phytoremediation of 2,4-Dichlorophenol","authors":"Zeynep Ceylan, M. Moharramzadeh, Ö. Atici","doi":"10.15671/HJBC.689446","DOIUrl":"https://doi.org/10.15671/HJBC.689446","url":null,"abstract":"In this work, the phytoremediation potential of 2,4-Dichlorophenol (2,4-DCP) from soil and wetlands by Datura stramonium L. (jimsonweed) was investigated. The medium of seedlings growing in a hydroponic system was adjusted to different concentrations (0.0, 75, 100, 125, 150, 175, 200, 225, 250 and 275 ppm) of 2,4-DCP. Four days later, the remediation rate of 2,4-DCP in the growth medium, and root-stem length, root-stem dry weight, lipid peroxidation (LPO), protein and photosynthetic pigment content of seedlings were evaluated. D. stramonium seedlings provided remediation of 2,4-DCP between 52-78% at all concentrations. In addition, the 2,4-DCP treatments inhibited the root-stem lengths and dry weights of seedlings compared to their controls, particularly at high doses such as 200-275 ppm, but not at low doses. The applications generally increased protein and LPO content of roots and leaves slightly, but did not affect chlorophyll. The results show that D. stramonium has a high usage potential for phytoremediation of 2,4-DCP.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90447605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis","authors":"M. Yaman, Erdinç Aladağ, H. M. Kayili, Y. Kadioglu, B. Salih","doi":"10.15671/HJBC.717600","DOIUrl":"https://doi.org/10.15671/HJBC.717600","url":null,"abstract":"Abstract Nowadays, the use of microalgae species as raw materials in biopharmaceutical production is on the agenda. The reason behind this idea is that microalgae are cell factories that are able to efficiently utilize carbon dioxide for the production of numerous biologically active compounds. However, there are several problems that remain to be solved in the production of recombinant protein from microalgaes. One of the critical requirements is to produce a bio-compatible N-glycosylation profile from the secreted recombinant proteins. However, the knowledge about the glycosylation machinery and N-glycan profiles of microalgae spices are quite limited. In the study, it was aimed to characterize N-glycan profiles of a green microalgae, Chlorella zofingiensis. To achieve this, photoautotrophically grown Chlorella zofingiensis extracts including (glyco-)proteins were enzymatically deglycosylated and labelled with 2-aminobenzoic acid tag. Released N-glycans were purified with a HILIC-based approach and analyzed by MALDI-TOF(/TOF)-MS. The results showed that C. zofingiensis included oligomannosidic type N-glycan patterns. In addition, N-glycosylation profiles of C. zofingiensis by MALDI-MS revealed that most of the oligomannosidic N-glycans were phosphorylated. Oz Gunumuzde, mikroalg turlerinin biyofarmasotik uretiminde hammadde olarak kullanimi konusu tartisilmaktadir. .Bu fikrin altinda yatan temel neden mikro alglerin, biyolojik olarak aktif cok sayida bilesenin uretimi icin karbon dioksiti etkin bir sekilde kullanabilen hucre fabrikalari olmasidir. Bununla birlikte, mikroalglerden rekombinant protein uretiminde kullanilmasi icin cozulmesi gereken birkac sorun mevcuttur. Kritik gereksinimlerden birisi salgilanan rekombinant proteinlerden biyo-uyumlu bir N-glikozilasyon profile uretmektir. Ancak mikroalg turlerinin glikozilasyon makineleri ve N-glikan profilleri hakkindaki bilgiler oldukca sinirlidir. Bu calismada, yesil bir mikroalg turu olan Chlorella zofingiensis ‘in N-glikan profilinin karakterize edilmesi amaclandi. Bu amacla (gliko-)proteinleri iceren fotoototrofik olarak yetistirilmis Chlorella zofingiensis ekstreleri enzimatik olarak deglikozile edildi ve 2-aminobenzoik asit etiketi ile etiketlendi. Serbest hale getirilen N-glikanlar HILIC bazli bir yaklasimla saflastirildi ve MALDI-TOF (/TOF)-MS ile analiz edildi. Sonuclar C. zofingiensis'in oligomannozidik turde N-glikan modellerini icerdigini gostermistir. Ek olarak, MALDI-MS tarafindan C. zofingiensis'in N-glikosilasyon profilleri, oligomannosidik N-glikanlarin neredeyse yarisinin fosforilenmis oldugunu ortaya koymustur.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89016598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenol adsorption on magnetic biochar derived from olive pomace: Equilibrium, kinetic and thermodynamics","authors":"G. Dinç, S. Yildiz, E. Yel","doi":"10.15671/hjbc.687387","DOIUrl":"https://doi.org/10.15671/hjbc.687387","url":null,"abstract":"I n this study, magnetic biochar obtained from pyrolysis of pretreated olive pomace by iron chloride was used as adsorbent to remove phenol and the adsorption capacity of phenol was revealed. Batch experiments were performed as a function of pH, contact time, adsorbent dosage, temperature, and phenol concentration. Moreover, adsorption kinetics and thermodynamics of phenol adsorption onto magnetic biochar were also evaluated in the study. The optimum conditions for maximum adsorption capacity were obtained at pH of 5.7, dosage of biochar 0.14 g and 60 minutes contact time. In this study, three adsorption isotherms, namely Langmuir, Freundlich and Temkin, were applied to fit the equilibrium data of adsorption of phenol onto magnetic biochar. Results showed that correlation coefficients (R 2 ) for three isotherm models decreased with the temperature increment from 20°C to 40°C and the most suitable isotherm model for adsorption was Freundlich. As for kinetics of the adsorption process, the best described model was found as pseudo-second order. In adsorption thermodynamics part, the negative ΔH° and ΔG° values demonstrated that adsorption was exothermic, feasible and was more spontaneous at lower temperatures.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"101 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77363920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chiral Separations by Capillary Electrophoresis and related Techniques with Different Chiral Selectors: A Review","authors":"Koray Şarkaya, Ilgım Göktürk, F. Yılmaz, Adil Denizli","doi":"10.15671/hjbc.815414","DOIUrl":"https://doi.org/10.15671/hjbc.815414","url":null,"abstract":"","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"113 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83464737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive Compound Activity Inducement of Thermophile Cyanobacterium aponinum Under Stress Conditions","authors":"N. Kiliç, G. Dönmez","doi":"10.15671/hjbc.696762","DOIUrl":"https://doi.org/10.15671/hjbc.696762","url":null,"abstract":"","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74519217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}