HAYATI Journal of Biosciences最新文献_第3页

Growth and Development Performance of Hermetia illucens L. (Diptera: Stratiomyidae) Larvae on Fermented Palm Kernel Meal (PKM) Substrate 发酵棕榈仁粕（PKM）基质上Hermetia illucens L.（Diptera: Stratiomyidae）幼虫的生长和发育表现

HAYATI Journal of Biosciences Pub Date : 2023-11-27 DOI: 10.4308/hjb.31.2.317-327

Naomi Florenata Damanik, R E Putra, I. Kinasih, A. Permana

{"title":"Growth and Development Performance of Hermetia illucens L. (Diptera: Stratiomyidae) Larvae on Fermented Palm Kernel Meal (PKM) Substrate","authors":"Naomi Florenata Damanik, R E Putra, I. Kinasih, A. Permana","doi":"10.4308/hjb.31.2.317-327","DOIUrl":"https://doi.org/10.4308/hjb.31.2.317-327","url":null,"abstract":"Larvae of Hermetia illucens (Black Soldier Fly/BSF) are considered agents of bioconversion of organic waste, including by-products of agroindustrial waste. Palm kernel meal (PKM), a palm oil mill waste contains high lignocellulose, making it difficult for BSF larvae to digest in which pre treatment process is required. This study aims to analyze the growth and development performance of BSF larvae in fermented PKM waste using EM4 and molasses as the pretreatment process. Five (5) days old BSF larvae were reared in PKM waste, which was fermented with EM4 and molasses for 2, 3, and 4 days (F2, F3, and F4) and with water for four days (FA) while chicken feed (PA) applied as control. During this study, growth rate, average weight, developmental period, survival rate, and larval development period. Feed efficiency and feed reduction analyzed by efficiency of conversion of digested food (ECD), feed conversion ratio (FCR), waste reduction index (WRI) and substrate consumption rate (SCR). The growth and development performance of F2 group was the best among other treatment groups which is similar to larvae fed on chicken feed as a control. All treatments showed high larval survival rates (99.72-100.00%). On the other hand, the best best reduction efficiency recorded in F3. Based on these result it can be concluded that pretreatment of PKM by EM4 and molasses is applicable to be improve the quality of PKM as feeding material for BSF larvae.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139230580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topical Application of Paraboea leuserensis on Excision Wound with Angiogenesis and Vascular Endothelial Growth Factor Analysis 对切除伤口局部应用 Paraboea leuserensis 的血管生成和血管内皮生长因子分析

HAYATI Journal of Biosciences Pub Date : 2023-11-21 DOI: 10.4308/hjb.31.2.300-316

Dyah Wijiana Heryani, Fiska Maya Wardhani, Yensuari, Nadya Nazimuddin Putri, Leo Nardi, Refi Ikhtiari

{"title":"Topical Application of Paraboea leuserensis on Excision Wound with Angiogenesis and Vascular Endothelial Growth Factor Analysis","authors":"Dyah Wijiana Heryani, Fiska Maya Wardhani, Yensuari, Nadya Nazimuddin Putri, Leo Nardi, Refi Ikhtiari","doi":"10.4308/hjb.31.2.300-316","DOIUrl":"https://doi.org/10.4308/hjb.31.2.300-316","url":null,"abstract":"Gagatan harimau or Paraboea leuserensis B.L. Burtt is an endemic plant that grows in limestone areas, natively found in Leuser Mountain Area, North Sumatra Province, Indonesia. This study determines the wound healing potential of P. leuserensis gel-based ethanolic extract (PGEE) on full-thickness excision wounds based on the angiogenesis and VEGF expression. Here, we reported 67 compounds detected by LC-MS/MS from the ethanolic extract. The antibacterial activity regarding pathogenic wound infection was also investigated. The experimental study used 30 Rattus novergicus, randomly divided into five treatment groups: base gel as a negative control, Bioplacenton as a positive control, PGEE 2.5%, 5%, and 10%. Experimental data showed that PGEE indicated a significant effect on bacterial inhibition (p = 0.02), where the greatest inhibitory was found against S. epidermidis. Topical application of PGEE showed significant differences in the number of blood vessels of PGEE 2.5% and 10% (p = 0.007) and expression of VEGF (p<0.05). The correlation between the number of blood vessels and VEGF was also described with the number of macrophages, fibroblasts, collagen, and wound diameter. Overall, this study demonstrates the pharmacological potential of PGEE for wound healing applications regarding angiogenesis and VEGF expression.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139253353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prospects of Indigenous Bacillus subtilis Strain LS9.1 as a Potential Biocontrol Agent against Aedes aegypti Larvae 本地枯草芽孢杆菌 LS9.1 菌株作为潜在生物控制剂防治埃及伊蚊幼虫的前景

HAYATI Journal of Biosciences Pub Date : 2023-11-20 DOI: 10.4308/hjb.31.2.293-299

Salamun Salamun, Ni’matuzahroh Ni’matuzahroh, Fatimah Fatimah, T. Nurhariyati, Agus Supriyanto, Izdihar Tsana, Farah Aisyah Nafidiastri

{"title":"Prospects of Indigenous Bacillus subtilis Strain LS9.1 as a Potential Biocontrol Agent against Aedes aegypti Larvae","authors":"Salamun Salamun, Ni’matuzahroh Ni’matuzahroh, Fatimah Fatimah, T. Nurhariyati, Agus Supriyanto, Izdihar Tsana, Farah Aisyah Nafidiastri","doi":"10.4308/hjb.31.2.293-299","DOIUrl":"https://doi.org/10.4308/hjb.31.2.293-299","url":null,"abstract":"Biocontrol is one way that can be developed to overcome public health and agricultural problems. Biocontrol agents could be a microorganism, for example, from the Bacillus group. This study aims to determine the larvicidal toxicity of the Bacillus subtilis strain LS9.1 against Aedes aegypti larvae. The mode of action of the isolate caused larval death, which was detected with parasporal inclusions and secondary metabolites. Larvicidal toxicity with the bioassay method. Parasporal inclusion proteins were detected with the SDS-PAGE method. The Cry toxin coding gene with the PCR method and cell ultrastructure with transmission and scanning electron microscopy (TEM and SEM). The action of secondary metabolites was detected with hemolytic activity test. The isolate high toxicity to A. aegypti larvae. Parasporal inclusion proteins with molecular weights ranging from 72-7 kDa and the gene encoding a protein toxin in size 300 bp. The TEM and SEM results did not Cry-IV toxin. The hemolytic activity test showed a positive reaction correlated with biosurfactant production. Based on this study, the death of larvae is probably caused by secondary metabolites produced by Bacillus subtilis strain LS9.1. Thus, this isolate has the prospect of being an environmentally friendly biocontrol agent to disease vectors and insect pests.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"112 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139259542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectivity of Silver Nanoparticles-Temu Giring (Curcuma heyneana) Rhizome on Inhibiting the Growth of Bacteria Causing Nosocomial Infection 纳米银颗粒-特木吉林（莪术）根茎对抑制引起医院感染的细菌生长的效果

HAYATI Journal of Biosciences Pub Date : 2023-11-15 DOI: 10.4308/hjb.31.2.284-292

A. M. Nurjamil, Renandy Kristianlie Ekajaya, William Junino Saputro, Zaki Fahreza Sururi, Diah Kusumawaty

{"title":"Effectivity of Silver Nanoparticles-Temu Giring (Curcuma heyneana) Rhizome on Inhibiting the Growth of Bacteria Causing Nosocomial Infection","authors":"A. M. Nurjamil, Renandy Kristianlie Ekajaya, William Junino Saputro, Zaki Fahreza Sururi, Diah Kusumawaty","doi":"10.4308/hjb.31.2.284-292","DOIUrl":"https://doi.org/10.4308/hjb.31.2.284-292","url":null,"abstract":"Biofilms are a common cause of nosocomial infections that often attack hospitalized patients. The main objective of this study was to examine the efficacy of silver nanoparticles-temu giring rhizomes in combating bacteria and preventing biofilm formation. The antibacterial and antibiofilm properties of these nanoparticles were evaluated against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The research began with the extraction of temu giring rhizome, synthesis of silver nanoparticles-temu giring rhizome, disk diffusion test, biofilm formation inhibitory activity test, and characterization of silver nanoparticles-temu giring rhizome. In this research, silver nanoparticles-temu giring rhizome were utilized at concentrations of 10, 20, 40, 80, 160 μg/ml, and a control in the form of chlorhexidine. The results showed that the silver nanoparticles-temu giring rhizome produced a larger inhibition zone for bacterial growth compared to the control against the three bacteria. The IC50 value of silver nanoparticles-temu giring rhizome required to inhibit biofilm formation was 27.64 μg/ml in E. coli, 29.29 μg/ml in P. aeruginosa, and 26.21 μg/ml in S. aureus. In P. aeruginosa, E. coli, and S. aureus, the IC50 for preventing biofilm formation by silver nanoparticles-temu giring rhizome was determined to be 27.64 μg/ml, 29.29 μg/ml, and 26.21 μg/ml, respectively. Evaluation of silver nanoparticles revealed the success of temu giring rhizomes in reducing silver ions. This is shown that silver nanoparticles-temu giring rhizomes can be developed into active ingredients that inhibit the growth of bacteria that cause nosocomial infections.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"192 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139272575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Toxicity Test of Synthetic Insecticides on Tetragonula laeviceps (Apidae: Meliponini) 人工合成杀虫剂对四角蛙（鳞翅目：Meliponini）的毒性试验

HAYATI Journal of Biosciences Pub Date : 2023-11-14 DOI: 10.4308/hjb.31.2.271-283

Ina Rubiatul Hasanah, N. Mubin, Dewi Sartiami, W. Priawandiputra, Dadang

{"title":"The Toxicity Test of Synthetic Insecticides on Tetragonula laeviceps (Apidae: Meliponini)","authors":"Ina Rubiatul Hasanah, N. Mubin, Dewi Sartiami, W. Priawandiputra, Dadang","doi":"10.4308/hjb.31.2.271-283","DOIUrl":"https://doi.org/10.4308/hjb.31.2.271-283","url":null,"abstract":"Intense insecticide application is thought to cause a decline in bee colonies worldwide. Bees are effective pollinators in increasing the production of agricultural commodities. The stingless bee Tetragonula laeviceps (Apidae: Meliponini) is widely cultivated and found around plantations. The insecticides used in the toxicity test were imidacloprid 200 g/L, fipronil 50 g/L, lambda-cyhalothrin 25 g/L, profenofos 500 g/L, and chlorantraniliprole 50 g/L followed by a semi-field test using imidacloprid, fipronil, and profenofos insecticides on the cucumber plant. The LC50 value showed that exposure to imidacloprid, fipronil, and profenofos insecticides caused toxic effects on T. laeviceps by contact and orally. Lambda-cyhalothrin was found harmful on contact exposure, in contrast, chlorantraniliprole was harmful through oral. Classification of insecticide toxicity based on LD50 contact for imidacloprid, fipronil, lambda-cyhalothrin, and profenofos were very toxic and needed a risk assessment. However, chlorantraniliprole was classified as moderately toxic and low risk. In semi-field test results, imidacloprid and fipronil insecticides caused a significant decrease in the leave-return and bee-visiting activity on cucumber flowers. These insecticides also produced a low average yield of fruit weight. Insecticide application can affect the role of T. laeviceps as the pollinator which impacts the production of agricultural commodities.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139276412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Salt and Heat Stress Trigger Morpho-Physiological Changes, Antioxidant Enzyme and Secondary Metabolites Gene Expression in Rice (Oryza sativa L.) 盐胁迫和热胁迫引发水稻（Oryza sativa L.）的形态生理变化、抗氧化酶和次生代谢物基因表达

HAYATI Journal of Biosciences Pub Date : 2023-11-14 DOI: 10.4308/hjb.31.2.256-270

Mohammad Ubaidillah, Noor Rozzita, Mitha Aprilia Mufadilah, Nurhaliza Thamrin, Agung Nugroho Puspito, Kyung Min Kim

{"title":"Salt and Heat Stress Trigger Morpho-Physiological Changes, Antioxidant Enzyme and Secondary Metabolites Gene Expression in Rice (Oryza sativa L.)","authors":"Mohammad Ubaidillah, Noor Rozzita, Mitha Aprilia Mufadilah, Nurhaliza Thamrin, Agung Nugroho Puspito, Kyung Min Kim","doi":"10.4308/hjb.31.2.256-270","DOIUrl":"https://doi.org/10.4308/hjb.31.2.256-270","url":null,"abstract":"Climate change significantly increases salt and heat stress in rice plants. This condition causes plants to activate antioxidant enzymes and produce secondary metabolites. This study aimed to determine the morpho-physiological changes and gene expression profiles of antioxidant enzymes and secondary metabolites. This study used a completely randomized design factorial. The first factor was local rice varieties (IR64, Silaun, and Cigeulis), and the second factor was stress treatments (control, NaCl 150 mM, 40°C, and NaCl 150 mM + 40°C). The results showed that multiple stress significantly affected the plant height, stem length, stem diameter, leaf area, root length, total main root, plant biomass, necrotic length, chlorophyll content, relative water content, and plant ROS production. Multiple stress could up-regulate the gene expression of antioxidant enzymes (Mn-SOD, Cu/Zn SOD, Cytosolic APX, OsAPX1, CAT, OsCATA, and GPOD) in rice after stress combination treatments and increase the secondary metabolites gene expression (P5CS and GABA-T) in all rice varieties. Still, the OsNOMT gene was only active in the Cigeulis variety.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139276918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning and Extracellular Expression of Glargine in Pichia pastoris 格拉尔金在 Pichia pastoris 中的克隆和细胞外表达

HAYATI Journal of Biosciences Pub Date : 2023-11-13 DOI: 10.4308/hjb.31.2.248-255

D. Hardianto, E. Martius, T. Rostinawati, A. Safarrida, J. I. Royani, F. Assyifa, D. Laziba

{"title":"Cloning and Extracellular Expression of Glargine in Pichia pastoris","authors":"D. Hardianto, E. Martius, T. Rostinawati, A. Safarrida, J. I. Royani, F. Assyifa, D. Laziba","doi":"10.4308/hjb.31.2.248-255","DOIUrl":"https://doi.org/10.4308/hjb.31.2.248-255","url":null,"abstract":"Patients with diabetes mellitus increase significantly every year. The increasing number of people with diabetes mellitus results in increased insulin requirements. There are two types of insulin used for diabetes mellitus treatment: human insulin and insulin analogues. Escherichia coli, Pichia pastoris, Saccharomyces cerevisiae, or Hansenula polymorpaha has been used to produce human insulin and insulin analogues. Pichia pastoris can produce glargine in large quantities, and the insulin protein produced will be secreted outside the cell to facilitate the purification process. The advantage of glargine has a long working time of up to 24 hours. Hence, glargine is more effective because patients with diabetes receive glargine injections only once daily. The research started with cloning the glargine gene, transforming pPICZαA-G plasmid into Pichia pastoris, and testing glargine production. 20 recombinant Pichia pastoris colonies were selected and regenerated. Eight recombinant Pichia pastoris colonies were tested for glargine production, and six colonies were detected producing glargine by electrophoresis SDS-PAGE gel stained with Coomassie blue. This study aims to produce glargine using Pichia pastoris as an expression system capable of producing glargine extracellularly, thus simplifying the purification process.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"37 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139278125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical, Antioxidant and Antimicrobial Activities of Hevea brasiliensis Leaves Extract 巴西红豆杉叶提取物的植物化学、抗氧化和抗菌活性

HAYATI Journal of Biosciences Pub Date : 2023-11-13 DOI: 10.4308/hjb.31.2.241-247

Kusumarn Noipha, Piyanuch Suwannarat, Supattra Prom-in, Titpawan Nakpheng

{"title":"Phytochemical, Antioxidant and Antimicrobial Activities of Hevea brasiliensis Leaves Extract","authors":"Kusumarn Noipha, Piyanuch Suwannarat, Supattra Prom-in, Titpawan Nakpheng","doi":"10.4308/hjb.31.2.241-247","DOIUrl":"https://doi.org/10.4308/hjb.31.2.241-247","url":null,"abstract":"Belonging to the Euphorbiaceae family, the Para rubber tree is formally referred to as Hevea brasiliensis in scientific terms. It is commonly known as an important economic commodity in Thailand because the natural rubber primarily originates from the milky latex obtained from the tree. However, the available research on the phytochemicals found in different parts of the rubber tree and their biological effects is quite restricted. This study aimed to determine the phytochemical constituents, antioxidant and antibacterial activity studies on the crude dry leaf extracts of H. brasiliensis. The results indicated the presence of alkaloids, anthraquinones, cardiac glycosides, coumarin, flavonoids, saponin, steroids, tannins, and terpenoids. The total phenolic content was 63.95±4.31 mgGAE/g in the ethanolic leaf extract. The ethanolic extract displayed notable effectiveness in scavenging free radicals (71.2±0.17%) at 500 μg/ml concentration and antioxidant capacity (the lowest IC50 value 42.57±0.91 μg/ml). The ethanol extract of the leaf of H. brasiliensis showed inhibition zone on all of the selected bacteria (gram-positive; Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, and gram-negative; Escherichia coli, Pseudomonas aeruginosa) at 200 mg/ml. In conclusion, the dried leaves of H. brasiliensis compose phytochemicals that exhibit antioxidant and antibacterial activities and possesses the potential to act as a reservoir of plant-derived antibiotics and natural antioxidants.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"15 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139278290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic and Genotypic Antimicrobial Resistance Profile of Salmonella spp. Isolated from Kampung Chicken Carcasses 从甘榜鸡屠体中分离出的沙门氏菌属的表型和基因型抗菌药耐药性概况

HAYATI Journal of Biosciences Pub Date : 2023-11-13 DOI: 10.4308/hjb.31.2.229-240

S. Nurjanah, W. Rahayu, Stephen Sanjaya

{"title":"Phenotypic and Genotypic Antimicrobial Resistance Profile of Salmonella spp. Isolated from Kampung Chicken Carcasses","authors":"S. Nurjanah, W. Rahayu, Stephen Sanjaya","doi":"10.4308/hjb.31.2.229-240","DOIUrl":"https://doi.org/10.4308/hjb.31.2.229-240","url":null,"abstract":"Kampung chicken meats have been widely consumed in Indonesia as well as broiler chicken. However, the extensive rearing allowed multidrug-resistant (MDR) bacteria exposure to kampung chicken, including through horizontal gene transfer. This study aimed to observe the correlation between the phenotypic and genotypic antimicrobial resistance profile of Salmonella spp. isolated from kampung chicken carcasses. Phenotypic antimicrobial resistance was evaluated by Kirby-Bauer's disk diffusion method, while the detection of drug resistance genes in seventeen isolates of Salmonella was carried out by PCR. All (17/17) isolates were susceptible to ampicillin and chloramphenicol. Most isolates of Salmonella were resistant to erythromycin (82%; 14/17), while the decreased susceptibility (intermediate category) most occurred in oxytetracycline (82%; 14/17). Salmonella Typhimurium showed a resistance pattern to more antimicrobial groups than S. Newport and S. Weltevreden. Several antimicrobial resistance genes (blaTEM, tetG, cmlA, gyrA) were present in all (17/17) isolates of Salmonella spp. Resistance to antimicrobial agents and the presence of resistance genes were not always related. This study could provide beneficial information regarding the transmission of antimicrobial resistance among Salmonella spp. from kampung chickens.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"34 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139278211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioethanol Production from Non-Conventional Yeasts Wickerhamomyces anomalus (Pichia anomala) and Detection of ADH1 Gene 用非常规酵母 Wickerhamomyces anomalus (Pichia anomala) 生产生物乙醇并检测 ADH1 基因

HAYATI Journal of Biosciences Pub Date : 2023-11-11 DOI: 10.4308/hjb.31.2.221-228

Muhammad Fadhil Fathiah, Faisal Diniamal Hartono, R. Astuti, Sri Listiyowati, A. Meryandini

{"title":"Bioethanol Production from Non-Conventional Yeasts Wickerhamomyces anomalus (Pichia anomala) and Detection of ADH1 Gene","authors":"Muhammad Fadhil Fathiah, Faisal Diniamal Hartono, R. Astuti, Sri Listiyowati, A. Meryandini","doi":"10.4308/hjb.31.2.221-228","DOIUrl":"https://doi.org/10.4308/hjb.31.2.221-228","url":null,"abstract":"Bioethanol is an organic compound resulted from the fermentation of sugar substrates by microorganisms which is used as alternative energy sources. During bioethanol fermentation yeast are exposed to various fermentation stresses, including temperature, osmotic, and oxidative stresess. Such conditions may decrease ethanol production. We previously isolated fermentation-stress tolerance yeast isolates from traditional Balinese beverages, identified as Wickerhamomyces anomalus BT2, BT5, and BT6. However no data available regarding the bioethanol production of those isolates. Our study indicates that these strains could utilize various sugar substrates (glucose, xylose, maltose, sucrose) in oxidative fermentative media. The highest value of substrate utilization efficiency following 48 hours fermentation was shown by BT6 on glucose (61.02%), BT 2 on xylose (55.44%) and maltose (60.90%). Measurement of ethanol production by Gas Chromatography showed that the strains were able to produce higher ethanol on the glucose substrate than other substrates. For instance, BT6 could produce the highest ethanol production (5.00 g/L) amongst strains tested by using glucose as substrate. Yet, the particular strains could only produce 0.30 g/L and 0.65 g/L by using xylose and maltose, respectively. For further genetic engineering purposes, we detected ADH1 gene from all three isolates, with high homology to the alcohol dehydrogenase from Saccharomyces cerevisiae, Geobacillus stearothermophilus and Pseudomonas aeruginosa. Further strain development can be carried out targeting the ADH1 gene, important for ethanol fermentation.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"31 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139280026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0