HAYATI Journal of Biosciences最新文献_第10页

Soybean Seeds (Glycine max L.) Extract Against Cytokine Storm in ARDS Rat Model through Inhibiting Inflammation Marker 大豆种子(甘氨酸max L.)通过抑制炎症标志物抑制ARDS大鼠模型细胞因子风暴

HAYATI Journal of Biosciences Pub Date : 2023-05-12 DOI: 10.4308/hjb.30.4.779-788

D. Priyandoko, W. Widowati, Ervi Afifah, Ika Adhani Sholihah, C. Wahyuni, C. Wijayanti, R. Rizal

引用次数: 0

UHPLC-Q-Orbitrap HRMS-based Untargeted Metabolomics of Sida rhombifolia Leaves and Stem Extracts 基于UHPLC-Q-Orbitrap HRMS的菱叶和茎提取物的非靶向代谢组学

HAYATI Journal of Biosciences Pub Date : 2023-05-11 DOI: 10.4308/hjb.30.4.770-778

Alfi Hudatul Karomah, M. Rafi, D. Septaningsih, A. Ilmiawati, Utami Dyah Safitri, N. Aminah, M. Insanu, A. Rohman

{"title":"UHPLC-Q-Orbitrap HRMS-based Untargeted Metabolomics of Sida rhombifolia Leaves and Stem Extracts","authors":"Alfi Hudatul Karomah, M. Rafi, D. Septaningsih, A. Ilmiawati, Utami Dyah Safitri, N. Aminah, M. Insanu, A. Rohman","doi":"10.4308/hjb.30.4.770-778","DOIUrl":"https://doi.org/10.4308/hjb.30.4.770-778","url":null,"abstract":"Sida rhombifolia, also known as sidaguri in Indonesia, is a medicinal plant commonly used as a herbal medicine because of its metabolite and biological activities. One of the several factors that affect plant metabolite composition and concentration is the use of plant parts. In this study, the experiment aimed to identify the metabolite profile in the leaves and stem extracts of S. rhombifolia using UHPLC-Q-Orbitrap HRMS-based untargeted metabolomics. The samples were distinguished by principal component analysis (PCA). Extraction of metabolites was conducted by sonication for approximately 30 min with 70% ethanol as the extraction solvent; 28 metabolites were identified. Seven metabolites were identified only in the leaves, three were identified only in the stems, and 18 other metabolites were identified in both the leaves and stems. These metabolites were categorized as flavonoids, triterpenoids, alkaloids, coumarins, phenolic aldehydes, phenolic acids, ecdysteroids, fatty acids, and monoterpene lactones. Based on the classification results, PCA grouped the leaves and stem extracts of S. rhombifolia using the peak area variables of the identified metabolites.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47623944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Records of Male and Swollen Females of Two Species of Achlysiella (Nematoda: Pratylenchidae) 两种Achlysiella雄性和肿胀雌性的首次记录（线虫目：Pratylenchidae）

HAYATI Journal of Biosciences Pub Date : 2023-05-10 DOI: 10.4308/hjb.30.4.757-769

A. Gafur, M. Hodda

引用次数: 0

Phylogeographic Insights of Five Co-Habiting Grouper Species in The Indo-Malaya Archipelago 印度-马来亚群岛五种共栖石斑鱼的系统地理学研究

HAYATI Journal of Biosciences Pub Date : 2023-05-02 DOI: 10.4308/hjb.30.4.743-756

N. Fadli, A. Damora, Z. Muchlisin, I. Dewiyanti, M. Ramadhaniaty, N. M. Razi, J. Jamaluddin, E. Macusi, M. N. Siti-Azizah

{"title":"Phylogeographic Insights of Five Co-Habiting Grouper Species in The Indo-Malaya Archipelago","authors":"N. Fadli, A. Damora, Z. Muchlisin, I. Dewiyanti, M. Ramadhaniaty, N. M. Razi, J. Jamaluddin, E. Macusi, M. N. Siti-Azizah","doi":"10.4308/hjb.30.4.743-756","DOIUrl":"https://doi.org/10.4308/hjb.30.4.743-756","url":null,"abstract":"Understanding the patterns of genetic diversity of species and precise stock identification are important in fisheries conservation management. However, studies on genetic diversity, connectivity, population structure, and gene flow of groupers within the Indo-Malaya Archipelago (IMA) waters are limited. The objective of the present study was to examine the phylogeographic patterns of the family Epinephelidae in IMA waters based on a parallel study of five selected species utilizing the mitochondrial COI. The grouper species were: areolate grouper (Epinephelus areolatus), blacktip grouper (Epinephelus fasciatus), six-bar grouper (Epinephelus sexfasciatus), blue-lined hind (Cephalopholis formosa), and white-edged lyretail (Variola albimarginata). Specimens were obtained from fish landing sites and fish markets from 23 locations throughout the IMA waters. This study showed genetic structuring for two species (Epinephelus areolatus and Variola albimarginata) but genetic homogeneity for the other three investigated species across IMA. Various geological and demographic histories, local and regional oceanographic features, and biological characteristics are hypothesized to shape the present genetic pattern of each species across the IMA waters. The establishment of effective international cooperation is encouraged to manage grouper species stocks in this region.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43572762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quercetin-Containing Extract from Clove Syzygium aromaticum L. Endophytic Bacteria, Fictibacillus phosphorivorans P1U2, Exhibits Antimutagenic Activity in Yeast Saccharomyces cerevisiae 含槲皮素的丁香香菇内生细菌P1U2对酵母的抗诱变活性研究

HAYATI Journal of Biosciences Pub Date : 2023-04-17 DOI: 10.4308/hjb.30.4.734-742

Nadila Fany Ashafani, R. Astuti, S. Listiyowati, Irmanida Batubara

{"title":"Quercetin-Containing Extract from Clove Syzygium aromaticum L. Endophytic Bacteria, Fictibacillus phosphorivorans P1U2, Exhibits Antimutagenic Activity in Yeast Saccharomyces cerevisiae","authors":"Nadila Fany Ashafani, R. Astuti, S. Listiyowati, Irmanida Batubara","doi":"10.4308/hjb.30.4.734-742","DOIUrl":"https://doi.org/10.4308/hjb.30.4.734-742","url":null,"abstract":"Metabolites from clove (Syzygium aromaticum L.) have been extensively studied for various pharmacological uses, including quercetin. Endophytic bacteria isolated from plant tissues can produce similar secondary metabolites as their host plants. However, little is known about the antioxidant activity of the endophytic bacterial extract from clove leaves. Amongst the potential bacterial groups, the genera of Bacillus have been widely studied as a source of antioxidants. Thus, this study, we examined the potential antioxidant properties of Bacillus sp., isolated from clove leaves. We have successfully isolated ten endophytic bacteria, of which only one isolate (P1U2) was found non-pathogenic based on a hemolytic assay. Based on the 16S rRNA sequence, P1U2 shared the highest similarity to Fictibacillus phosphorivorans. The extract of extracellular metabolites of P1U2 contains quercetin compounds, based on LC-MS analysis. The results of the antioxidant activity test using the DPPH assay showed that the P1U2 extract had an IC50 value of 161 mg/ml. The supplementation of the extract on culture media reduced the ability of S. cerevisiae to decolorize mutagen malachite green and increased cell viability. Furthermore, the addition of 161 ppm extract was able to counteract UV mutagens effects of UV exposure and toxicity of malachite green, significantly increasing the growth of S. cerevisiae. Suggesting the bioactivity of the extract in combating mutagens. To the best of our knowledge, this is the first study to report a quercetin-rich extract derived from endophytic bacteria, F. phosphorivorans. Thus, further study is required to optimize the synthesis of quercetin from isolate P1U2.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":"4 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41299776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Extensive Coverage Anoa Distribution Modelling in Sulawesi Using Maximum Entropy 使用最大熵的苏拉威西大面积覆盖Anoa分布模型

HAYATI Journal of Biosciences Pub Date : 2023-04-17 DOI: 10.4308/hjb.30.4.716-724

L. M. Jaelani, Benedict, Diah Ardiani, M. P. Tambunan, M. Indrawan, Andri A. Wibowo

{"title":"An Extensive Coverage Anoa Distribution Modelling in Sulawesi Using Maximum Entropy","authors":"L. M. Jaelani, Benedict, Diah Ardiani, M. P. Tambunan, M. Indrawan, Andri A. Wibowo","doi":"10.4308/hjb.30.4.716-724","DOIUrl":"https://doi.org/10.4308/hjb.30.4.716-724","url":null,"abstract":"As an endangered species, Anoa (Bubalus depressicornis and Mountain Bubalus quarlesi) inhabiting the Sulawesi island requires proper conservation both in and out of their native habitat. The study of anoa habitat is mainly conducted through field studies based on firsthand observations of anoa appearance, footprints, or excrement or through social surveys from residents who saw it directly. The studies are carried out specifically in a particular area with a relatively narrow. However, in practice, this method has limitations, such as the research location determined based on the possibility of anoa, limited research area, and inefficient use of resources. Therefore, this study aimed to model the potential habitat of anoa in the whole of Sulawesi island. This study was based on physical and environmental independent variables such as DEM, surface slope, LST, NDVI, and access to inland water, as well as in-situ species distribution retrieved from scientific papers and reports. This study discovered the likely anoa distribution on Sulawesi island, both inside and outside of its native habitat. LST is the most important independent variable in determining habitat suitability, accounting for 80% of the total, followed by water (15.3%), NDVI (2.9%), DEM (1.6%), and slope (0.3%).","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45497658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Patterns of Restriction Fragment of Several Enzymes to Distinguish Toxoplasma gondii Isolates Virulent and Avirulent Strains using GRA1 and GRA7 Genetic Marker 利用GRA1和GRA7遗传标记区分刚地弓形虫毒株和无毒株的几种酶限制性片段模式

HAYATI Journal of Biosciences Pub Date : 2023-04-17 DOI: 10.4308/hjb.30.4.725-733

F. Ekawasti, Eko Setyo Purwanto, Farlin Nepho, D. A. Kurniawati, D. Subekti, R. Damayanti, S. Sa’diah, U. Cahyaningsih

{"title":"The Patterns of Restriction Fragment of Several Enzymes to Distinguish Toxoplasma gondii Isolates Virulent and Avirulent Strains using GRA1 and GRA7 Genetic Marker","authors":"F. Ekawasti, Eko Setyo Purwanto, Farlin Nepho, D. A. Kurniawati, D. Subekti, R. Damayanti, S. Sa’diah, U. Cahyaningsih","doi":"10.4308/hjb.30.4.725-733","DOIUrl":"https://doi.org/10.4308/hjb.30.4.725-733","url":null,"abstract":"Toxoplasma gondii pathogenicity depends on the type derived from a clonal population. A genetic analysis of the locus has been carried out to determine the different genotypes of T. gondii (strain types I, II, and III) that are associated with human toxoplasmosis. The several genotypes of T. gondii (strain types I, II, and III) that are linked to human toxoplasmosis have been identified through genetic study of the locus. In this investigation, PCR-RFLP was found to be a useful, and simple method genotypic characterization. The objective of this study was to genotyping characterize T. gondii RH and BEV strains isolates by PCR-RFLP using several restriction enzymes. T. gondii tachyzoite DNA was extracted and amplified by PCR using dense granule genetic markers (GRA1 and GRA7) designed with Primer3plus. The amplification were digested using the restriction enzymes. The PCR-RFLP amplified dense granule products was used to classify strains into two genotypes of T. gondii (virulent and avirulent). The results demonstrated that the RFLP patterns of the GRA1 and GRA7 gene area digested by DdeI, MvaI, HinfI, RsaI, and Sau96I enzymes can be used to identify virulent or avirulent strains of T. gondii. Toxoplasma gondii RH and BEV strain produced different digestion product which can be used to distinguished the strains.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46252736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Combination of Anadara nodifera Shell and Milkfish Thorns Powder Effectively Promote Springiness Index, Serum Testosterone, and Breast Muscle Testosterone in Bangkok Rooster Anadara nodifera Shell和Milkfish Thorns Powder的组合有效促进曼谷公鸡的弹性指数、血清睾酮和胸肌睾酮

HAYATI Journal of Biosciences Pub Date : 2023-04-12 DOI: 10.4308/hjb.30.4.701-710

Akhmad Abror As Sidiqi, C. M. Airin, Sarmin Sarmin, P. Astuti

{"title":"A Combination of Anadara nodifera Shell and Milkfish Thorns Powder Effectively Promote Springiness Index, Serum Testosterone, and Breast Muscle Testosterone in Bangkok Rooster","authors":"Akhmad Abror As Sidiqi, C. M. Airin, Sarmin Sarmin, P. Astuti","doi":"10.4308/hjb.30.4.701-710","DOIUrl":"https://doi.org/10.4308/hjb.30.4.701-710","url":null,"abstract":"Aromatase blocker effectively promotes testosterone, which improves muscle performance and protein synthesis. Considerable muscle strength in Bangkok rooster is the outcome of testosterone activity. Clamshell contains aromatase blocker substances, while fishbone possesses a high amount of protein. This research aimed to evaluate the clamshell and fishbone combination potency towards testosterone levels and muscle performance in Bangkok roosters. This study employed 18 Bangkok roosters under 35 days of treatment. The rooster groups consisted of control (P0, n = 6); clamshell-fishbone (P1, n = 6): clamshell (6.6 gram/day) + fishbone (3.3 gram/day); fishbone (P2, n = 6): fishbone (3.3 gram/day). The blood was collected once every seven days. Enzyme immunoassay showed the highest serum and muscle testosterone levels in P1 (p<0.05). Immunohistochemistry presented the most and broadest myofiber and the highest proliferation activity in P1 (p<0.05). The texture analysis showed the topmost springiness index in P1 (p<0.05). Aromatase blockers in clamshell and protein in fishbone improve testosterone and muscle performance in Bangkok roosters.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43390494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological Alterations of Fish Erythrocytes as Their Response to Environmental Conditions 鱼类红细胞对环境条件的形态学改变

HAYATI Journal of Biosciences Pub Date : 2023-04-12 DOI: 10.4308/hjb.30.4.711-715

D. H. Quyet, P. T. Dung

引用次数: 0

Bird Sounds Psychological Restorative Effect on The Visitors of Bogor Botanical Garden 鸟叫声对茂物植物园游客的心理修复作用

HAYATI Journal of Biosciences Pub Date : 2023-04-11 DOI: 10.4308/hjb.30.4.692-700

Yuriko Asahiro, A. Sunkar, Jarwadi Budi Hernowo

{"title":"Bird Sounds Psychological Restorative Effect on The Visitors of Bogor Botanical Garden","authors":"Yuriko Asahiro, A. Sunkar, Jarwadi Budi Hernowo","doi":"10.4308/hjb.30.4.692-700","DOIUrl":"https://doi.org/10.4308/hjb.30.4.692-700","url":null,"abstract":"Stress can be defined as psychological and physical tension caused by physical, emotional, social, economic, and occupational, events, or experiences that are difficult to manage or endure. Bird sounds can be a potential solution to reduce stress levels. This study aimed to analyze the effect of bird sounds as a stress restorative on Bogor Botanical Garden visitors. A survey of 100 visitors of Bogor Botanical Garden was conducted based on the Perceived Stress Scale and Perceived Restorativeness Soundscape Scale. Data on respondent characteristics were analyzed using descriptive analysis, the relationship between stress levels and perceived stress restorative effect of bird sound was analyzed using Pearson correlation analysis. The analysis showed that 78% of respondents had moderate to high perceived stress levels and 98% of respondents rated the degree of restoration from bird sounds to be moderate to high. The correlation analysis revealed a negative relationship between the stress level and bird sounds' perceived restorative effect. The older the respondents and the more frequently they visited Bogor Botanical Garden, the higher the perceived stress restorative effect from bird sound.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44259165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0